scholarly journals MiRNAs Regulating Oxidative Stress: A Correlation with Doppler Sonography of Uteroplacental Complex and Clinical State Assessments of Newborns in Fetal Growth Restriction

2020 ◽  
Vol 9 (10) ◽  
pp. 3227
Author(s):  
Vladislava Gusar ◽  
Mariya Ganichkina ◽  
Vitaliy Chagovets ◽  
Nataliya Kan ◽  
Gennadiy Sukhikh
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Fetal Growth ◽  
Umbilical Cord Blood ◽  
Fetal Growth Restriction ◽  
Early Onset ◽  
Umbilical Artery ◽  
Late Onset ◽  
Perinatal Outcomes

Overproduction of reactive oxygen species (ROS) and, as a result, uncontrolled oxidative stress (OS) can play a central role in disorders of fetal hemodynamics and subsequent development of adverse perinatal outcomes in newborns with fetal growth restriction (FGR). Given the epigenetic nature of such disorders, the aim of our study was to evaluate the expression of miRNAs associated with OS and endothelial dysfunction (miR-27a-3p, miR-30b-5p, miR-125b-5p, miR-221-3p, miR-451a and miR-574-3p) in umbilical cord blood using real-time quantitative RT-PCR. ΜiRNA expression was evaluated in patients with FGR delivery before (n = 9 pregnant) and after 34 weeks of gestation (n = 13 pregnant), and the control groups corresponding to the main groups by gestational age (13 pregnant women in each group, respectively). A significant increase in miR-451a expression was detected in late-onset FGR and correlations with fetoplacental and cerebral circulation were established (increase of resistance in the umbilical artery (pulsatility index, PI UA (umbilical artery): r = −0.59, p = 0.001) and a decrease in cerebral blood flow (CPR: r = 0.48, p = 0.009)). The change in miR-125b-5p expression in the placenta is associated with reduced Doppler of cerebral hemodynamics (CPR: r = 0.73, p = 0.003; PI MCA (middle cerebral artery): r = 0.79, p = 0.0007), and newborn weight (r = 0.56, p = 0.04) in early-onset FGR. In addition, significant changes in miR-125b-5p and miR-451a expression in umbilical cord blood plasma were found in newborns with neonatal respiratory distress syndrome (NRDS) (in early-onset FGR) and very low birth weight (VLBW) (in late-onset FGR). A number of key signaling pathways have been identified in which the regulation of the studied miRNAs is involved, including angiogenesis, neurotrophin signaling pathway and oxidative stress response. In general, our study showed that changes of the redox homeostasis in the mother-placenta-fetus system in FGR and subsequent perinatal outcomes may be due to differential expression of oxidative stress-associated miRNAs.

Prognostic value of the aortic isthmus Doppler assessment on late onset fetal growth restriction

2019 ◽  
Vol 47 (2) ◽  
pp. 212-217
Author(s):  
Cecilia Villalaín ◽  
Ignacio Herraiz ◽  
Maria S. Quezada ◽  
Paula I. Gómez-Arriaga ◽  
Elisa Simón ◽  
...  
Keyword(s):  
Vaginal Delivery ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Umbilical Artery ◽  
Late Onset ◽  
Perinatal Outcomes ◽  
Labor Induction ◽  
Growth Restriction ◽  
Reversed Flow ◽  
Aortic Isthmus

AbstractBackgroundAs conflicting results have been reported about the association of reversed flow on the aortic isthmus (AoI) and adverse perinatal results in fetal growth restriction (FGR), we aim to compare perinatal outcomes (including tolerance to labor induction) of late-onset FGR between those with anterograde and reversed AoI flow.MethodsThis was an observational retrospective cohort study on 148 singleton gestations diagnosed with late-onset FGR (diagnosis ≥32+0 weeks), with an estimated fetal weight (EFW) <10thcentile and mild fetal Doppler alteration: umbilical artery (UA) pulsatility index (PI) >95thcentile, middle cerebral artery (MCA)-PI <5thcentile or cerebral-placental ratio <5thcentile. Anterograde AoI flow was present in n=79 and reversed AoI flow in n=69. Delivery was recommended from 37 weeks in both groups. Perinatal results were compared between the groups.ResultsThe global percentage of vaginal delivery of fetuses with anterograde and reversed blood flow was 55.7% vs. 66.7% (P=0.18) and the percentage of cesarean section (C-section) for non-reassuring fetal status was 12.7% vs. 15.9% (P=0.29), respectively. When evaluating those that underwent labor induction, the vaginal delivery rate was 67.9% vs. 77.2% (P=0.17), respectively. There were no significant differences regarding any other perinatal variables and there were no cases of severe morbidity or mortality.ConclusionWe observed that the presence of reversed AoI flow does not worsen perinatal outcomes on fetuses with late-onset growth restriction with mild Doppler alterations. Attempt of labor induction is feasible in these fetuses regardless of the direction of AoI flow.

Cord blood oxidative stress markers correlate with umbilical artery pulsatility in fetal growth restriction

2012 ◽  
Vol 26 (6) ◽  
pp. 576-580 ◽  
Author(s):  
Emine Seda Guvendag Guven ◽  
Deniz Karcaaltincaba ◽  
Omer Kandemir ◽  
Sadiman Kiykac ◽  
Ahmet Mentese
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Umbilical Artery ◽  
Growth Restriction ◽  
Oxidative Stress Markers ◽  
Stress Markers

Maternal Serum and Umbilical Cord Blood Leptin Concentrations With Fetal Growth Restriction

2003 ◽  
Vol 102 (3) ◽  
pp. 535-543
Author(s):  
Marcella Pighetti ◽  
Giovanni A. Tommaselli ◽  
Antonio DʼElia ◽  
Costantino Di Carlo ◽  
Angela Mariano ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Fetal Growth ◽  
Umbilical Cord Blood ◽  
Fetal Growth Restriction ◽  
Maternal Serum ◽  
Growth Restriction

scholarly journals Differential expression of markers of oxidative stress and apoptosis in relation to serum ferritin levels in patients with pre-eclampsia

BioMedica ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 1-8
Author(s):  
Jianying Yan ◽  
Jie Dong ◽  
Xiaoqian Lin ◽  
Lichun Chen ◽  
Zhuanji Fang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Serum Ferritin ◽  
Umbilical Cord Blood ◽  
Cell Apoptosis ◽  
Late Onset ◽  
Placental Tissue ◽  
Control Group ◽  
Pregnant Females

<p><strong>Background and Objective:</strong> Pre-eclampsia (PE) is a hypertensive gestational disease appearing during second trimester of pregnancy. Free radicals are released by the placenta in this condition that may cause oxidative damage. This study was designed to determine the serum ferritin (SF) levels in maternal blood, fetal umbilical cord blood and placenta and the changes associated with oxidative stress as well as cell apoptosis to understand the pathogenesis of PE.</p> <p><strong>Methods:</strong> This cross-sectional analytical study recruited 60 pregnant females with severe PE and assigned into early and late onset PE groups. Another n = 60 cases of normal pregnant females with similar gestational weeks were selected in the control group. Maternal serum and fetal umbilical cord blood ferritin levels were determined by automatic biochemical immunoassay system. Reverse transcription real-time fluorescence, Western blot and colorimetry were used to determine mRNA expression levels of ferritin and ferritin heavy chain, relative expression of ferritin and superoxide dismutase, malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) levels, respectively.</p> <p><strong>Results:</strong> Mean age was 30.89 &plusmn; 5.65 and 31.79 &plusmn; 5.06 years in early and late onset PE groups respectively. Serum uric acid and creatinine levels of both PE groups were significantly higher than the normal pregnant females. SF levels were also higher in the participants of PE groups. However, the mRNA and ferritin protein levels in placental tissue were significantly lower in PE groups while comparing to controls. The cleaved caspase-3 protein, GSH-Px and MDA levels were significantly higher in both PE groups (p &lt; 0.05).</p> <p><strong>Conclusion:</strong> The alterations in factors related to oxidative stress and cell apoptosis in placental tissue may be helpful to understand the pathogenesis of PE and may provide potential biomarkers for the diagnosis of PE.</p>

Iron metabolism disorder and oxidative damage levels in placenta are involved in preeclampsia

2021 ◽  
Author(s):  
Jianying Yan ◽  
Jie Dong ◽  
Xiaoqian Lin ◽  
Lichun Chen ◽  
Zhuanji Fang ◽  
...  
Keyword(s):  
Cord Blood ◽  
Pregnant Women ◽  
Blood Sample ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Early Onset ◽  
Late Onset ◽  
Control Group ◽  
Cord Blood Sample ◽  
Stress Injury

IntroductionTo explore the role of ferritin in placenta, serum and umbilical cord blood of pregnant women and the changes of oxidative stress injury as well as cell apoptosis in placenta in the pathogenesis of preeclampsia (PE).Material and methodsSixty pregnant women with severe PE were assigned into early-onset and late-onset PE group. Another 60 cases of normal late pregnant women with similar gestational weeks were divided into early-onset and late-onset control group. Maternal serum and fetal umbilical cord blood ferritin content was determined by automatic biochemical immunoassay system; mRNA expression levels of ferritin and ferritin heavy chain (FTH) were detected by reverse transcription real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). Western Blot was used to detect the relative expression level of ferritin and apoptosis; the contents of total superoxide dismutase (T-SOD) and malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were detected by colorimetry.ResultsSerum uric acid (UA) and creatinine (Cr) levels of PE groups were significantly higher when compared to the controls. The serum ferritin levels in blood sample and umbilical cord blood sample were significantly higher relative to the controls. However, the mRNA and protein levels of ferritin levels in placenta samples were significantly lower compared with the controls. The placental cleaved caspase-3, Bcl-2 levels were significantly lower than the early onset PE group. The levels of GSH-Px and MDA in placenta were significantly higher.ConclusionsThese results may assist understanding the pathogenesis of PE and provide potential biomarkers for diagnosis of PE.

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