Iron metabolism disorder and oxidative damage levels in placenta are involved in preeclampsia

IntroductionTo explore the role of ferritin in placenta, serum and umbilical cord blood of pregnant women and the changes of oxidative stress injury as well as cell apoptosis in placenta in the pathogenesis of preeclampsia (PE).Material and methodsSixty pregnant women with severe PE were assigned into early-onset and late-onset PE group. Another 60 cases of normal late pregnant women with similar gestational weeks were divided into early-onset and late-onset control group. Maternal serum and fetal umbilical cord blood ferritin content was determined by automatic biochemical immunoassay system; mRNA expression levels of ferritin and ferritin heavy chain (FTH) were detected by reverse transcription real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). Western Blot was used to detect the relative expression level of ferritin and apoptosis; the contents of total superoxide dismutase (T-SOD) and malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were detected by colorimetry.ResultsSerum uric acid (UA) and creatinine (Cr) levels of PE groups were significantly higher when compared to the controls. The serum ferritin levels in blood sample and umbilical cord blood sample were significantly higher relative to the controls. However, the mRNA and protein levels of ferritin levels in placenta samples were significantly lower compared with the controls. The placental cleaved caspase-3, Bcl-2 levels were significantly lower than the early onset PE group. The levels of GSH-Px and MDA in placenta were significantly higher.ConclusionsThese results may assist understanding the pathogenesis of PE and provide potential biomarkers for diagnosis of PE.

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Discovery of a novel HLA class I allele, HLA‐B*38:75 , in an Indian umbilical cord blood sample

HLA ◽

10.1111/tan.13660 ◽

2019 ◽

Vol 94 (5) ◽

pp. 442-443 ◽

Cited By ~ 2

Author(s):

Satyen Y. Sanghavi ◽

Tripti U. Gaunkar ◽

Vinayak V. Kedage

Keyword(s):

Cord Blood ◽

Blood Sample ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Hla Class I ◽

Class I ◽

Cord Blood Sample ◽

Umbilical Cord Blood Sample

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Higher Levels of Triglyceride, Fatty Acid Translocase, and Toll-Like Receptor 4 and Lower Level of HDL-C in Pregnant Women with GDM and Their Close Correlation with Neonatal Weight

Gynecologic and Obstetric Investigation ◽

10.1159/000510032 ◽

2021 ◽

pp. 1-7

Author(s):

Jianli Zhou ◽

Jie Bai ◽

Yanjuan Guo ◽

Lijun Fu ◽

Jun Xing

Keyword(s):

Cord Blood ◽

Pregnant Women ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Venous Blood ◽

Density Lipoprotein ◽

Maternal Blood ◽

Control Group ◽

Case Group ◽

Toll Like Receptor 4

Objectives: In this study, we aimed to compare the levels of maternal blood lipids, placental and venous blood lipid transporters, and inflammatory factor receptors in pregnant women with and without gestational diabetes mellitus (GDM). We also aimed to figure out the relationship between these values and neonatal weight. Methods: Fifty pregnant women with GDM under blood glucose control belong to the case group, and 50 pregnant women with normal glucose tolerance in concurrent delivery belong to the control group. Fasting venous blood of these pregnant women was taken 2 weeks before delivery, and umbilical cord blood was collected after delivery. The levels of triglyceride (TG), serum total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol (HDL-C) in maternal blood and umbilical cord blood were tested in the laboratory department of our hospital. The level of toll-like receptor 4 (TLR4) in serum of umbilical veins was detected by the double-antibody sandwich ELISA. Western blot and RT-PCR were used to detect the protein and mRNA expressions of TLR4, LPL, and FAT/CD36 in the placenta. Results: The level of TG in maternal blood in the case group was remarkably higher than that in the control group, which was opposite to the level of HDL-C. In the umbilical cord blood of women with GDM, the expression of TLR4 increased and was closely correlated with neonatal weight. In the placenta of women with GDM, the expressions of FAT/CD36 and TLR4 increased, and both of them were closely correlated with neonatal weight. Besides, TLR4 in umbilical cord blood increased and was closely correlated with neonatal weight. Although the expression of LPL in the placenta decreased, it had no obvious correlation with neonatal weight. Conclusions: TG in maternal blood, TLR4 in the placenta and umbilical cord blood, and FAT/CD36 in the placenta were positively correlated with neonatal weight. However, HDL-C in maternal blood was negatively correlated with neonatal weight. Although the expression of LPL in the placenta reduced due to GDM, it had no correlation with neonatal weight.

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Differential expression of markers of oxidative stress and apoptosis in relation to serum ferritin levels in patients with pre-eclampsia

BioMedica ◽

10.51441/biomedica/5-512 ◽

2021 ◽

Vol 37 (3) ◽

pp. 1-8

Author(s):

Jianying Yan ◽

Jie Dong ◽

Xiaoqian Lin ◽

Lichun Chen ◽

Zhuanji Fang ◽

...

Keyword(s):

Oxidative Stress ◽

Cord Blood ◽

Umbilical Cord ◽

Serum Ferritin ◽

Umbilical Cord Blood ◽

Cell Apoptosis ◽

Late Onset ◽

Placental Tissue ◽

Control Group ◽

Pregnant Females

Background and Objective: Pre-eclampsia (PE) is a hypertensive gestational disease appearing during second trimester of pregnancy. Free radicals are released by the placenta in this condition that may cause oxidative damage. This study was designed to determine the serum ferritin (SF) levels in maternal blood, fetal umbilical cord blood and placenta and the changes associated with oxidative stress as well as cell apoptosis to understand the pathogenesis of PE. Methods: This cross-sectional analytical study recruited 60 pregnant females with severe PE and assigned into early and late onset PE groups. Another n = 60 cases of normal pregnant females with similar gestational weeks were selected in the control group. Maternal serum and fetal umbilical cord blood ferritin levels were determined by automatic biochemical immunoassay system. Reverse transcription real-time fluorescence, Western blot and colorimetry were used to determine mRNA expression levels of ferritin and ferritin heavy chain, relative expression of ferritin and superoxide dismutase, malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) levels, respectively. Results: Mean age was 30.89 ± 5.65 and 31.79 ± 5.06 years in early and late onset PE groups respectively. Serum uric acid and creatinine levels of both PE groups were significantly higher than the normal pregnant females. SF levels were also higher in the participants of PE groups. However, the mRNA and ferritin protein levels in placental tissue were significantly lower in PE groups while comparing to controls. The cleaved caspase-3 protein, GSH-Px and MDA levels were significantly higher in both PE groups (p < 0.05). Conclusion: The alterations in factors related to oxidative stress and cell apoptosis in placental tissue may be helpful to understand the pathogenesis of PE and may provide potential biomarkers for the diagnosis of PE.

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The Correlations between Cord Blood Leptin and Leptin Level at Six Months with Infant Growth

Journal of Biomedicine and Translational Research ◽

10.14710/jbtr.v7i2.11821 ◽

2021 ◽

Vol 7 (2) ◽

pp. 74-78

Author(s):

Ika Rara Rosita ◽

Agustini Utari ◽

Maria Mexitalia

Keyword(s):

Cord Blood ◽

Blood Sample ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Nutrient Intake ◽

Infant Growth ◽

Cord Blood Sample ◽

Elisa Method ◽

The Mean ◽

The Relationship

Background: Leptin plays an important role in regulating body weight, metabolism, and reproductive functions. Leptin affects metabolism by reducing nutrient intake and increasing energy expenditure which eventually also plays a role in infant growth.Objective: This study aims to determine the relationship between leptin levels and infant growth age 0-6 months.Methods: A prospective cohort study was done for six months on 38 infants, age 0-6 months, from breastfeeding mothers with normal pregnancies. The samples were taken twice, firstly when the infant was born using an umbilical cord blood sample, and secondly at the age of six months, using a vein blood sample. Serum leptin levels were measured using the ELISA method. Infant growth was assessed using WHO 2005’s z-scores.Results: A total of 50 babies were included in the study, 38 of them had been studied completely. Significant correlations were found between the mean of the umbilical cord and six months of age leptin levels (p <0.001), between delta leptin with WHZ and delta leptin with WAZ at six months of age (p = 0.002 and p = 0.003, respectively), and between leptin levels with WHZ (p<0.001) and leptin levels with WAZ (p = 0.004) at six months of age. Leptin levels at the age of six months are lower than umbilical cord blood leptin. Conclusion: The greater decrease of leptin level in the first six months is associated with better infant growth.

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MiRNAs Regulating Oxidative Stress: A Correlation with Doppler Sonography of Uteroplacental Complex and Clinical State Assessments of Newborns in Fetal Growth Restriction

Journal of Clinical Medicine ◽

10.3390/jcm9103227 ◽

2020 ◽

Vol 9 (10) ◽

pp. 3227

Author(s):

Vladislava Gusar ◽

Mariya Ganichkina ◽

Vitaliy Chagovets ◽

Nataliya Kan ◽

Gennadiy Sukhikh

Keyword(s):

Oxidative Stress ◽

Cord Blood ◽

Umbilical Cord ◽

Fetal Growth ◽

Umbilical Cord Blood ◽

Fetal Growth Restriction ◽

Early Onset ◽

Umbilical Artery ◽

Late Onset ◽

Perinatal Outcomes

Overproduction of reactive oxygen species (ROS) and, as a result, uncontrolled oxidative stress (OS) can play a central role in disorders of fetal hemodynamics and subsequent development of adverse perinatal outcomes in newborns with fetal growth restriction (FGR). Given the epigenetic nature of such disorders, the aim of our study was to evaluate the expression of miRNAs associated with OS and endothelial dysfunction (miR-27a-3p, miR-30b-5p, miR-125b-5p, miR-221-3p, miR-451a and miR-574-3p) in umbilical cord blood using real-time quantitative RT-PCR. ΜiRNA expression was evaluated in patients with FGR delivery before (n = 9 pregnant) and after 34 weeks of gestation (n = 13 pregnant), and the control groups corresponding to the main groups by gestational age (13 pregnant women in each group, respectively). A significant increase in miR-451a expression was detected in late-onset FGR and correlations with fetoplacental and cerebral circulation were established (increase of resistance in the umbilical artery (pulsatility index, PI UA (umbilical artery): r = −0.59, p = 0.001) and a decrease in cerebral blood flow (CPR: r = 0.48, p = 0.009)). The change in miR-125b-5p expression in the placenta is associated with reduced Doppler of cerebral hemodynamics (CPR: r = 0.73, p = 0.003; PI MCA (middle cerebral artery): r = 0.79, p = 0.0007), and newborn weight (r = 0.56, p = 0.04) in early-onset FGR. In addition, significant changes in miR-125b-5p and miR-451a expression in umbilical cord blood plasma were found in newborns with neonatal respiratory distress syndrome (NRDS) (in early-onset FGR) and very low birth weight (VLBW) (in late-onset FGR). A number of key signaling pathways have been identified in which the regulation of the studied miRNAs is involved, including angiogenesis, neurotrophin signaling pathway and oxidative stress response. In general, our study showed that changes of the redox homeostasis in the mother-placenta-fetus system in FGR and subsequent perinatal outcomes may be due to differential expression of oxidative stress-associated miRNAs.

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The Correlation of Antibacterial Peptides Concentration in Umbilical Cord Blood and Early-Onset Sepsis in Preterm Infants

10.21203/rs.3.rs-233772/v1 ◽

2021 ◽

Author(s):

Jiayu Miao ◽

Ying Liu ◽

Zengqing Li ◽

Zhuxiao Ren ◽

Zhicheng Zhong ◽

...

Keyword(s):

Cord Blood ◽

Preterm Infants ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Early Onset ◽

Sepsis Group ◽

Control Group ◽

Early Onset Sepsis ◽

Prediction Probability

Abstract Background: The role of serum LL37 in systemic inflammation has been confirmed, and the influence of it in umbilical cord blood to early-onset sepsis in preterm infants is currently being investigated.Results: The level of LL37 of sepsis group was higher than those of in control group (362.13±46.71 vs 248.13±83.30 ng/ml), the levels of CRP, WBC and MPV in sepsis group were higher than those of in control group (6.25±4.19 vs 2.89±2.77 mg/L; 17.60±12.35 vs 8.24±3.55×109/L; 11.10±1.11 vs 8.93±0.68 fL), the level of PLT was lower than those of in control group (175.20±38.51 vs 245.75±49.85×109/L) (P < 0.05). The expression of LL37 was negatively correlated with PLT (r = -0.9347, P < 0.0001), and positively correlated with MPV ((r =0.9463 , P < 0.0001), the expression of PLT was negatively correlated with MPV (r = -0.9641, P < 0.0001). The area under curve of LL37 for diagnosis of early-onset sepsis was 0.875, the prediction probability was 0.7, the sensitivity was 90.0% and the specificity was 80.0%.Conclusions：The higher level of LL37 in umbilical cord blood was associated with the development of early-onset sepsis in preterm infants.

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Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

The Tohoku Journal of Experimental Medicine ◽

10.1620/tjem.215.363 ◽

2008 ◽

Vol 215 (4) ◽

pp. 363-371 ◽

Cited By ~ 5

Author(s):

Darko Grujicic ◽

Olivera Miloševic-Djordjevic ◽

Slobodan Arsenijevic ◽

Dragoslav Marinkovic

Keyword(s):

Cord Blood ◽

Pregnant Women ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Blood Lymphocytes ◽

Cord Blood Lymphocytes

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Correlation between maternal and umbilical cord blood in pregnant women of Pokhara Valley: a cross sectional study

BMC Pregnancy and Childbirth ◽

10.1186/s12884-018-1697-1 ◽

2018 ◽

Vol 18 (1) ◽

Cited By ~ 2

Author(s):

Sameer Timilsina ◽

Sirisa Karki ◽

Aajeevan Gautam ◽

Pujan Bhusal ◽

Gita Paudel ◽

...

Keyword(s):

Cord Blood ◽

Pregnant Women ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Pokhara Valley

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Correlation between Intrapartum Cardiotocogram findings and cord blood pH in term and preterm labors

Current Women s Health Reviews ◽

10.2174/1573404817666210811124304 ◽

2021 ◽

Vol 17 ◽

Author(s):

Shubha Rao ◽

Himanshi Jain ◽

Anjali Suneel ◽

Roopa Padavagodu Shivananda ◽

Akhila Vasudeva

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Preterm Labor ◽

Fetal Monitoring ◽

Cord Blood Sample ◽

Arterial Blood ◽

Blood Ph ◽

Preterm Group ◽

Fetal Acidosis

Background: The purpose of intrapartum fetal monitoring by cardiotocograph (CTG) is to identify early signs of developing hypoxia so that appropriate action can be taken to improve the perinatal outcome. Although CTG findings are well known to monitor the progress of the labor due to the paucity of recommendations, there has always been a clinical dilemma as the term fetuses respond differently than a preterm fetus. However, umbilical cord blood pH can distinguish the infant at high risk for asphyxia and related sequel. Therefore, because of differences in fetal physiology in term and preterm fetuses, CTG findings vary, and hence the validity of CTG to determine fetal acidosis should be different. Aims and Objectives: This study aimed to correlate abnormal intrapartum CTG findings with umbilical cord blood pH in term and preterm labor and thus evaluate the success of CTG in predicting fetal acidosis during labor. Methods: The present study included 210 women in labor (70 preterm and 140 term) with abnormal intrapartum CTG that was classified as per 2015 revised International Federation of Gynecologists and Obstetrician (FIGO) guidelines. Immediately after delivery 2 ml Umbilical artery cord blood sample was taken in a pre-heparinized syringe for analysis, pH <=7.2 was taken as acidosis and pH >7.2 was taken as normal. The measured data were maternal general characteristics which included gravida status, associated comorbidities, method of induction and character of liquor, the intrapartum CTG tracings recorded the cord arterial blood pH and the neonatal characteristics such as APGAR score and neonatal outcome. Results: Data from 70 preterm labor was compared with 140 term labor. In this study, 20.9 % of the babies had acidosis. Suspicious CTG due to decreased variability were more common in the preterm group than in the term group (21.4% vs. 8.6% p<0.05). Positive predictive value (PPV) of abnormal CTG for fetal acidosis in the preterm group was found to be higher than that in term group, PPV of pathological CTG being even higher than suspicious CTG. Women with suspicious CTG had 82 % less risk of fetal acidosis as compared to pathological CTG. Women with Bradycardia had 5.9 times the risk of fetal acidosis as compared with normal and tachycardia. Conclusion: Abnormal CTG should be managed appropriately without any delay to prevent acidosis and cord blood pH should be done in all labors with abnormal CTG. However, our findings of a higher incidence of lower cord blood pH and suspicious CTG due to decreased variability alone, highlight the limitation of criteria currently used for interpretation of CTG in preterm labors.

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