scholarly journals Differential expression of markers of oxidative stress and apoptosis in relation to serum ferritin levels in patients with pre-eclampsia

BioMedica ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 1-8
Author(s):  
Jianying Yan ◽  
Jie Dong ◽  
Xiaoqian Lin ◽  
Lichun Chen ◽  
Zhuanji Fang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Serum Ferritin ◽  
Umbilical Cord Blood ◽  
Cell Apoptosis ◽  
Late Onset ◽  
Placental Tissue ◽  
Control Group ◽  
Pregnant Females

<p><strong>Background and Objective:</strong> Pre-eclampsia (PE) is a hypertensive gestational disease appearing during second trimester of pregnancy. Free radicals are released by the placenta in this condition that may cause oxidative damage. This study was designed to determine the serum ferritin (SF) levels in maternal blood, fetal umbilical cord blood and placenta and the changes associated with oxidative stress as well as cell apoptosis to understand the pathogenesis of PE.</p> <p><strong>Methods:</strong> This cross-sectional analytical study recruited 60 pregnant females with severe PE and assigned into early and late onset PE groups. Another n = 60 cases of normal pregnant females with similar gestational weeks were selected in the control group. Maternal serum and fetal umbilical cord blood ferritin levels were determined by automatic biochemical immunoassay system. Reverse transcription real-time fluorescence, Western blot and colorimetry were used to determine mRNA expression levels of ferritin and ferritin heavy chain, relative expression of ferritin and superoxide dismutase, malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) levels, respectively.</p> <p><strong>Results:</strong> Mean age was 30.89 &plusmn; 5.65 and 31.79 &plusmn; 5.06 years in early and late onset PE groups respectively. Serum uric acid and creatinine levels of both PE groups were significantly higher than the normal pregnant females. SF levels were also higher in the participants of PE groups. However, the mRNA and ferritin protein levels in placental tissue were significantly lower in PE groups while comparing to controls. The cleaved caspase-3 protein, GSH-Px and MDA levels were significantly higher in both PE groups (p &lt; 0.05).</p> <p><strong>Conclusion:</strong> The alterations in factors related to oxidative stress and cell apoptosis in placental tissue may be helpful to understand the pathogenesis of PE and may provide potential biomarkers for the diagnosis of PE.</p>

scholarly journals MiRNAs Regulating Oxidative Stress: A Correlation with Doppler Sonography of Uteroplacental Complex and Clinical State Assessments of Newborns in Fetal Growth Restriction

2020 ◽  
Vol 9 (10) ◽  
pp. 3227
Author(s):  
Vladislava Gusar ◽  
Mariya Ganichkina ◽  
Vitaliy Chagovets ◽  
Nataliya Kan ◽  
Gennadiy Sukhikh
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Fetal Growth ◽  
Umbilical Cord Blood ◽  
Fetal Growth Restriction ◽  
Early Onset ◽  
Umbilical Artery ◽  
Late Onset ◽  
Perinatal Outcomes

Overproduction of reactive oxygen species (ROS) and, as a result, uncontrolled oxidative stress (OS) can play a central role in disorders of fetal hemodynamics and subsequent development of adverse perinatal outcomes in newborns with fetal growth restriction (FGR). Given the epigenetic nature of such disorders, the aim of our study was to evaluate the expression of miRNAs associated with OS and endothelial dysfunction (miR-27a-3p, miR-30b-5p, miR-125b-5p, miR-221-3p, miR-451a and miR-574-3p) in umbilical cord blood using real-time quantitative RT-PCR. ΜiRNA expression was evaluated in patients with FGR delivery before (n = 9 pregnant) and after 34 weeks of gestation (n = 13 pregnant), and the control groups corresponding to the main groups by gestational age (13 pregnant women in each group, respectively). A significant increase in miR-451a expression was detected in late-onset FGR and correlations with fetoplacental and cerebral circulation were established (increase of resistance in the umbilical artery (pulsatility index, PI UA (umbilical artery): r = −0.59, p = 0.001) and a decrease in cerebral blood flow (CPR: r = 0.48, p = 0.009)). The change in miR-125b-5p expression in the placenta is associated with reduced Doppler of cerebral hemodynamics (CPR: r = 0.73, p = 0.003; PI MCA (middle cerebral artery): r = 0.79, p = 0.0007), and newborn weight (r = 0.56, p = 0.04) in early-onset FGR. In addition, significant changes in miR-125b-5p and miR-451a expression in umbilical cord blood plasma were found in newborns with neonatal respiratory distress syndrome (NRDS) (in early-onset FGR) and very low birth weight (VLBW) (in late-onset FGR). A number of key signaling pathways have been identified in which the regulation of the studied miRNAs is involved, including angiogenesis, neurotrophin signaling pathway and oxidative stress response. In general, our study showed that changes of the redox homeostasis in the mother-placenta-fetus system in FGR and subsequent perinatal outcomes may be due to differential expression of oxidative stress-associated miRNAs.

Iron metabolism disorder and oxidative damage levels in placenta are involved in preeclampsia

2021 ◽  
Author(s):  
Jianying Yan ◽  
Jie Dong ◽  
Xiaoqian Lin ◽  
Lichun Chen ◽  
Zhuanji Fang ◽  
...  
Keyword(s):  
Cord Blood ◽  
Pregnant Women ◽  
Blood Sample ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Early Onset ◽  
Late Onset ◽  
Control Group ◽  
Cord Blood Sample ◽  
Stress Injury

IntroductionTo explore the role of ferritin in placenta, serum and umbilical cord blood of pregnant women and the changes of oxidative stress injury as well as cell apoptosis in placenta in the pathogenesis of preeclampsia (PE).Material and methodsSixty pregnant women with severe PE were assigned into early-onset and late-onset PE group. Another 60 cases of normal late pregnant women with similar gestational weeks were divided into early-onset and late-onset control group. Maternal serum and fetal umbilical cord blood ferritin content was determined by automatic biochemical immunoassay system; mRNA expression levels of ferritin and ferritin heavy chain (FTH) were detected by reverse transcription real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). Western Blot was used to detect the relative expression level of ferritin and apoptosis; the contents of total superoxide dismutase (T-SOD) and malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were detected by colorimetry.ResultsSerum uric acid (UA) and creatinine (Cr) levels of PE groups were significantly higher when compared to the controls. The serum ferritin levels in blood sample and umbilical cord blood sample were significantly higher relative to the controls. However, the mRNA and protein levels of ferritin levels in placenta samples were significantly lower compared with the controls. The placental cleaved caspase-3, Bcl-2 levels were significantly lower than the early onset PE group. The levels of GSH-Px and MDA in placenta were significantly higher.ConclusionsThese results may assist understanding the pathogenesis of PE and provide potential biomarkers for diagnosis of PE.

scholarly journals Umbilical Cord Serum Ferritin Concentration is Inversely Associated with Umbilical Cord Hemoglobin in Neonates Born to Adolescents Carrying Singletons and Women Carrying Multiples

2019 ◽  
Vol 149 (3) ◽  
pp. 406-415 ◽  
Author(s):  
Katherine M Delaney ◽  
Ronnie Guillet ◽  
Robert E Fleming ◽  
Yuan Ru ◽  
Eva K Pressman ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Serum Ferritin ◽  
Umbilical Cord Blood ◽  
Serum Iron ◽  
Iron Status ◽  
Inverse Association ◽  
Ferritin Concentration ◽  
Mediation Analyses ◽  
Significant Inverse Association

ABSTRACT Background It has been proposed that the fetus prioritizes iron for hemoglobin production over delivery to tissues. However, few studies have evaluated the interrelations between hemoglobin and multiple iron status biomarkers in umbilical cord blood. A full understanding is needed of how these parameters influence each other within cord blood to fully interpret iron and hematologic status at birth. Objectives We evaluated the determinants of neonatal hemoglobin and assessed the interrelations between hemoglobin, serum iron status indicators, and serum iron regulatory hormones in healthy neonates. Methods This was an observational study that assessed umbilical cord hemoglobin (Hb), serum ferritin (SF), erythropoietin (EPO), soluble transferrin receptor (sTfR), serum iron, hepcidin, vitamin B-12, folate, IL-6, and CRP measured in 234 neonates born to adolescents or to women carrying multiples. Correlations between these indicators were evaluated and mediation models consistent with the observed significant determinants of cord Hb concentrations were developed. Results A highly significant inverse association was found between cord SF and Hb concentrations that was not attributable to neonatal or maternal inflammation (as measured by IL-6 and CRP). The inverse association was present in the combined cohort, as well as in the adolescent and multiples cohorts independently. Mediation analyses found that EPO and hepcidin had significant indirect effects on cord Hb, associations that are explicable by mediation through SF and sTfR. Conclusion In contrast to observations made in older infants, a highly significant inverse association between Hb and SF, as well positive associations between Hb and both sTfR and EPO, were observed in umbilical cord blood from neonates born to adolescents or women carrying multiples. These findings, combined with review of the published literature, indicate a need for analysis of the relations between multiple parameters to assess iron and hematologic status at birth. These clinical trials were registered at clinicaltrials.gov as NCT01582802 (https://clinicaltrials.gov/ct2/show/NCT01582802) and NCT01019902 (https://clinicaltrials.gov/ct2/show/NCT01019902)

Comparison of angiogenic and anti-angiogenic factors in maternal and umbilical cord blood in early- and late-onset pre-eclampsia

2012 ◽  
Vol 28 (8) ◽  
pp. 628-632 ◽  
Author(s):  
Selda Demircan Sezer ◽  
Mert Küçük ◽  
Çiğdem Yenisey ◽  
Hasan Yüksel ◽  
Ali Rıza Odabaşı ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Late Onset ◽  
Angiogenic Factors

Umbilical cord blood markers of oxidative stress in pregnancies complicated by preterm prelabor rupture of membranes

2015 ◽  
Vol 29 (12) ◽  
pp. 1900-1910 ◽  
Author(s):  
Ivana Musilova ◽  
Lubomira Tothova ◽  
Ramkumar Menon ◽  
Barbora Vlkova ◽  
Peter Celec ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Markers Of Oxidative Stress ◽  
Prelabor Rupture Of Membranes

Correlation between circulating biomarkers of oxidative stress of maternal and umbilical cord blood at birth

2006 ◽  
Vol 40 (6) ◽  
pp. 565-570 ◽  
Author(s):  
Sandro Argüelles ◽  
Maria José Machado ◽  
Antonio Ayala ◽  
Alberto Machado ◽  
Blas Hervías
Keyword(s):  
Oxidative Stress ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Circulating Biomarkers ◽  
Biomarkers Of Oxidative Stress

scholarly journals The content of gas transmitters in umbilical cord blood and blood of newborns born to mothers with preeclampsia

2021 ◽  
Vol 66 (4) ◽  
pp. 53-57
Author(s):  
I. G. Popova ◽  
O. G. Sitnikova ◽  
S. B. Nazarov ◽  
G. N. Kuzmenko ◽  
M. M. Klychevа ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Life Support ◽  
Venous Blood ◽  
The Body ◽  
Control Group ◽  
Control Groups ◽  
And Control

To assess endothelial function in the newborns born to mothers with preeclampsia and without preeclampsia the scientists assessed the content of nitricoxide and hydrogen sulfide in the umbilical and venous blood of these children. The main group consisted of 80 women with moderate preeclampsia and their80 newborns; the control group included 50 children born to women without preeclampsia. The authors studied the content of nitric oxide by determining the totalnitrates and nitrites (NOx), hydrogen sulfide in mixed cord blood collected within 30 minutes after delivery, and venous blood taken from newborns on the 1-3rd day of life. Umbilical cord blood demonstrated no significant differences in the content of NO xand hydrogen sulfide in newborns of the main and control groups. However, in general, the study of umbilical cord blood revealed a relationship between the concentration of hydrogen sulfide and the possibility of developing intraventricular hemorrhagein a newborn of the main and control groups. In the venous blood there was a significant increase in the content of NOx and hydrogen sulfide in newborns born to mothers with preeclampsia. It may cause vasodilation asa compensatory reaction of the body during the period of postnatal adaptation of the child’s body, aimed at life support.

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