Occurrence and Morpho-Molecular Identification of Botryosphaeriales Species from Guizhou Province, China

Botryosphaeriales is an important order of diverse fungal pathogens, saprobes, and endophytes distributed worldwide. Recent studies of Botryosphaeriales in China have discovered a broad range of species, some of which have not been formerly described. In this study, 60 saprobic isolates were obtained from decaying woody hosts in southwestern China. The isolates were compared with other species using morphological characteristics, and available DNA sequence data was used to infer phylogenetic analyses based on the internal transcribed spacer (ITS), large subunit rRNA gene (LSU), and translation elongation factor 1-α (tef) loci. Three novel species were illustrated and described as Botryobambusa guizhouensis, Sardiniella elliptica, and Sphaeropsis guizhouensis, which belong to rarely identified genera within Botryosphaeriaceae. Botryobambusa guizhouensis is the second species identified from the respective monotypic genus. The previously known species were identified as Aplosporella hesperidica, Barriopsis tectonae, Botryosphaeria dothidea, Diplodia mutila, Di. neojuniperi, Di. pseudoseriata, Di. sapinea, Di. seriata, Dothiorella sarmentorum, Do. yunnana, Lasiodiplodia pseudotheobromae, Neofusicoccum parvum, Sardiniella celtidis, Sa. guizhouensis, and Sphaeropsis citrigena. The results of this study indicate that numerous species of Botryosphaeriales are yet to be revealed in southwestern China.

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Four new species in the Tremella fibulifera complex (Tremellales, Basidiomycota)

MycoKeys ◽

10.3897/mycokeys.82.63241 ◽

2021 ◽

Vol 82 ◽

pp. 33-56

Author(s):

Long-Fei Fan ◽

Renato Lúcio Mendes Alvarenga ◽

Tatiana Baptista Gibertoni ◽

Fang Wu ◽

Yu-Cheng Dai

Keyword(s):

New Species ◽

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Molecular Data ◽

Small Subunit ◽

Molecular Evidence ◽

Rrna Gene

Samples of species close to Tremella fibulifera from China and Brazil are studied, and T. fibulifera is confirmed as a species complex including nine species. Five known species (T. cheejenii, T. fibulifera s.s., T. “neofibulifera”, T. lloydiae-candidae and T. olens) and four new species (T. australe, T. guangxiensis, T. latispora and T. subfibulifera) in the complex are recognized based on morphological characteristics, molecular evidence, and geographic distribution. Sequences of eight species of the complex were included in the phylogenetic analyses because T. olens lacks molecular data. The phylogenetic analyses were performed by a combined sequence dataset of the internal transcribed spacer (ITS) and the partial nuclear large subunit rDNA (nLSU), and a combined sequence dataset of the ITS, partial nLSU, the small subunit mitochondrial rRNA gene (mtSSU), the translation elongation factor 1-α (TEF1), the largest and second largest subunits of RNA polymerase II (RPB1 and RPB2). The eight species formed eight independent lineages with robust support in phylogenies based on both datasets. Illustrated description of the six species including Tremella fibulifera s.s., T. “neofibulifera” and four new species, and discussions with their related species, are provided. A table of the comparison of the important characteristics of nine species in the T. fibulifera complex and a key to the whitish species in Tremella s.s. are provided.

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Dothidea kunmingensis, a novel asexual species of Dothideaceae on Jasminum nudiflorum (winter jasmine) from Southwestern China

Phytotaxa ◽

10.11646/phytotaxa.529.1.3 ◽

2021 ◽

Vol 529 (1) ◽

pp. 43-56

Author(s):

YING GAO ◽

JUTAMART MONKAI ◽

ELENI GENTEKAKI ◽

GUANG-CONG REN ◽

DHANUSHKA N. WANASINGHE ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Small Subunit ◽

Morphological Characterization ◽

Small Subunit Rrna ◽

Sister Relationship ◽

Large Subunit Rrna

During a survey of saprobic microfungi in Southwest China, a coelomycetous fungus was found on dead twigs of Jasminum nudiflorum in Kunming, Yunnan Province. Based on a detailed morphological characterization coupled with multi-locus phylogenetic analyses, the fungus was identified as a new species in the genus Dothidea. Phylogenetic analyses using a combined matrix consisting of internal transcribed spacer (ITS), large subunit rRNA (LSU), small subunit rRNA (SSU), beta tubulin (tub2) and translation elongation factor-1 alpha (tef1-α) confirmed its placement in Dothideaceae and revealed a sister relationship to Dothidea eucalypti. The new species is characterized by pycnidial conidiomata, ampulliform or doliiform conidiogenous cells as well as aseptate, subglobose to ovoid, hyaline to pale-brown conidia. Comprehensive descriptions and illustrations are provided. Morphological characteristics of asexual morph taxa in Dothideaceae are also summarized and discussed.

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Moelleriella sinensis sp. nov. (Clavicipitaceae, Ascomycota) from southeast China

Phytotaxa ◽

10.11646/phytotaxa.429.4.5 ◽

2020 ◽

Vol 429 (4) ◽

pp. 289-296

Author(s):

YU-XI CHEN ◽

QING-LIN XUE ◽

YONG-XIAO XIE ◽

NEMAT O. KEYHANI ◽

XIA-YU GUAN ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characterization ◽

Rrna Gene ◽

Distinct Lineage ◽

Spore Mass ◽

Large Subunit Rrna ◽

A New Species

Moelleriella sinensis, a fungal pathogen infecting whitefly nymphs (Hemiptera), is described and illustrated as a new species from Wu Yi Mountain in Fujian province, southeastern China. This species is characterized by producing a pale brown spore mass around whitish to pale yellow pulvinate stroma. In surveys of entomopathogenic fungal diversity, only the anamorphic state was found in collected stroma. Phylogenetic analyses using the nucleotide sequences derived from the nuclear ribosomal large subunit rRNA gene, RNA polymerase subunit 1, and translation elongation factor 1α, coupled to morphological characterization supports the placement of the isolate as a new species in a distinct lineage within Moelleriella.

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Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control

Microorganisms ◽

10.3390/microorganisms8050689 ◽

2020 ◽

Vol 8 (5) ◽

pp. 689 ◽

Cited By ~ 3

Author(s):

Wenjun Li ◽

Ming Hu ◽

Yang Xue ◽

Zhijun Li ◽

Yanfei Zhang ◽

...

Keyword(s):

Ribosomal Rna ◽

Fungal Pathogens ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Its Region ◽

Small Subunit ◽

Field Application ◽

Financial Loss ◽

Twig Blight

Bayberry (Myrica rubra) is a commercial fruit in China. For the past seven years, twig blight disease has been attacking bayberry plantations in Shantou City, Guangdong Province, China, leading to destructive damage and financial loss. In this study, five fungal species associated with twig dieback and stem blight were identified based on morphological characteristics combined with multilocus sequence analysis (MLSA) on the internal transcribed spacer (ITS) region, partial sequences of β-tubulin (tub2), translation elongation factor 1-α (tef1-α), large subunit ribosomal RNA (LSU) and small subunit ribosomal RNA (SSU) genes, which are Epicoccum sorghinum, Neofusicoccum parvum, Lasiodiplodia theobromae, Nigrospora oryzae and a Pestalotiopsis new species P. myricae. P. myricae is the chief pathogen in fields, based on its high isolation rate and fast disease progression after inoculation. To our knowledge, this is the first study reporting the above five fungi as the pathogens responsible for bayberry twig blight. Indoor screening of fungicides indicates that Prochloraz (copper salt) is the most promising fungicide for field application, followed by Pyraclostrobin, 15% Difenoconazole + 15% Propiconazole, Difenoconazole and Myclobutanil. Additionally, Bacillus velezensis strain 3–10 and zeamines from Dickeya zeae strain EC1 could be used as potential ecofriendly alternatives to control the disease.

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Schistonchus hirtus n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus hirta in China

Nematology ◽

10.1163/138855409x12571623969727 ◽

2010 ◽

Vol 12 (4) ◽

pp. 543-556 ◽

Cited By ~ 7

Author(s):

Yongsan Zeng ◽

Weimin Ye ◽

Robin M. Giblin-Davis ◽

Changhui Li ◽

Zhijian Du ◽

...

Keyword(s):

Sequence Data ◽

Large Subunit ◽

Morphological Characters ◽

Small Subunit ◽

Rrna Gene ◽

Small Subunit Rrna ◽

Wasp Species ◽

Dna Sequence Data ◽

Ficus Hirta ◽

Large Subunit Rrna

Abstract A nematode recovered from syconia of Ficus hirta from Guangzhou, P. R. China, during a survey of nematode biodiversity from 2007 to 2009, is described herein as Schistonchus hirtus n. sp. and is differentiated by a combination of morphological characters, including excretory pore (EP) located near the metacorpus, a short post-uterine sac (PUS) (0.5 vulval body diam. (VBD) long), rose thorn-shaped spicules, amoeboid sperm, absence of gubernaculum, three pairs of subventral papillae on the male tail, host-Ficus and host-wasp species and DNA sequence data. Morphologically, S. hirtus n. sp. is close to S. centerae, S. altermacrophylla, S. aureus, S. laevigatus and S. virens based upon the length of the PUS (about 0.5 VBD long). However, the relative position of the EP in S. hirtus n. sp. is very different from these species (near metacorpus vs near head). With regard to the EP character, S. hirtus n. sp. is very similar to S. macrophylla, S. guangzhouensis and S. caprifici where the EP is at metacorpus level. However, S. hirtus n. sp. differs from S. macrophylla and S. guangzhouensis by possessing a shorter PUS and smaller spicules, and differs from S. caprifici by a shorter female stylet and smaller spicules. Schistonchus hirtus n. sp. was easily differentiated from other sequenced species by the proportion of parsimony informative changes in the partial small subunit rRNA gene (SSU) and D2/D3 expansion segments of the large subunit rRNA gene (LSU). Phylogenetic analysis with SSU sequences suggests that S. hirtus n. sp. is in a highly supported monophyletic clade with Aphelenchoides and Laimaphelenchus and is polyphyletic to other sequenced Schistonchus species. With LSU sequence data, it forms a clade with S. caprifici and they appear polyphyletic relative to S. guangzhouensis, S. centerae, S. aureus, S. laevigatus and S. virens.

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Molecular and morphological evidence indicates that Pseudorhabdosynochus lantauensis (Monogenea: Diplectanidae) represents two species

Parasitology ◽

10.1017/s0031182004007152 ◽

2005 ◽

Vol 130 (6) ◽

pp. 669-677 ◽

Cited By ~ 25

Author(s):

X. Y. WU ◽

N. B. CHILTON ◽

X. Q. ZHU ◽

M. Q. XIE ◽

A. X. LI

Keyword(s):

Sequence Data ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Strong Support ◽

Morphological Characteristics ◽

Sufficient Evidence ◽

Bootstrap Support ◽

Morphological Evidence ◽

Lsu Rdna ◽

Epinephelus Coioides

Sequences of the first internal transcribed spacer (ITS-1) and the D1-D3 domains of the large subunit (LSU) of the ribosomal DNA (rDNA) were determined for multiple specimens of 4 operational taxonomic units (OTUs) of the monogenean, Pseudorhabdosynochus lantauensis. OTUs were defined based on their collecting localities, host and/or morphological characteristics. All P. lantauensis specimens of one group (OTUs 1 and 3) differed in their sequences of the ITS-1 and partial LSU rDNA when compared with specimens of a second group (OTUs 2 and 4) by 12% and 2%, respectively. Results of the phylogenetic analyses of the LSU rDNA sequence data showed total (100%) bootstrap support for the separation of P. lantauensis into 2 distinct clades. At least 11 of the 18 nucleotide differences in the LSU sequence between the two P. lantauensis clades were derived (i.e. autapomorphic) characters when the morphologically distinct species, P. epinepheli and P. coioidesis, were used as outgroups. Furthermore, there were several autapomorphic character states for each P. lantauensis clade. This provides sufficient evidence to reject the null hypothesis that P. lantauensis represents a single species. Morphological and morphometric differences between these two clades provided additional strong support for the separation of P. lantauensis into two species. These two parasite species were found to co-exist on one of the two species of serranid fish (i.e. Epinephelus coioides) examined in the South China Sea (Guangdong Province, China).

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Zygotorulaspora dagestanica sp. nov., a novel ascomycetous yeast species associated with the Georgian honeysuckle (Lonicera iberica M. Bieb.)

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijsem.0.004785 ◽

2021 ◽

Vol 71 (4) ◽

Author(s):

Aleksey V. Kachalkin ◽

Dinakhanum A. Abdullabekova ◽

Elena S. Magomedova ◽

Andrey M. Yurkov

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Its Region ◽

Rrna Gene ◽

Ascomycetous Yeast ◽

Wild Fruit ◽

Large Subunit Rrna ◽

A New Species ◽

Basal Position

During an investigation of the yeast communities associated with wild fruit shrubs in Dagestan (Caucasus, Russia), four fermenting ascospore-producing yeast strains were isolated from leaves of the Georgian honeysuckle (Lonicera iberica M. Bieb.) and from soil underneath this plant. Phylogenetic analyses based on concatenated sequences of the ITS region and D1/D2 domains of the large subunit rRNA gene and concatenated sequences of the ribosomal DNA cystron, RPB2 and TEF1 genes showed that the isolated strains represented a new species of the genus Zygotorulaspora. The new species was placed in the basal position to other species of the clade and close to Zygotorulaspora mrakii. Based on the results of phylogenetic analyses and the phenotypic characteristics of the four studied strains, a novel species is described, for which the name Zygotorulaspora dagestanica sp. nov. is proposed. The holotype is KBP Y-4591T, three metabolically inactive cryopreserved isotype cultures are DSM 100088, VKM Y-3060 and VKPM Y-4318. The MycoBank number is MB 838285.

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Morphology and phylogeny of Pseudorobillarda eucalypti sp. nov., from Thailand

Phytotaxa ◽

10.11646/phytotaxa.176.1.24 ◽

2014 ◽

Vol 176 (1) ◽

pp. 251 ◽

Cited By ~ 3

Author(s):

NARUMON TANGTHIRASUNUN ◽

PHILIPPE SILAR ◽

DARBHE JAYARAMA BHAT ◽

EKACHAI CHUKEATIROTE ◽

D.N. NALIN WIJAYAWARDENE ◽

...

Keyword(s):

Rna Polymerase Ii ◽

Ribosomal Dna ◽

Phylogenetic Trees ◽

Sequence Data ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Distinct Species ◽

Small Subunit ◽

Family Status

Pseudorobillarda is a coelomycete genus of Dothideomycetes with appendaged conidia and 15 species epithets. In this study, we isolated four strains of Pseudorobillarda from dead leaves in Thailand. DNA sequence data generated from the large subunit (28S) ribosomal DNA (nuLSU) gene was used in phylogenetic studies. The phylogenetic trees generated indicate that Pseudorobillarda form a distinct lineage in Dothideomycetes that may eventually require separate family status. The Pseudorobillarda strains comprised two distinct species and this is also supported by morphological characteristics. In this paper we introduce a new species of Pseudorobillarda, P. eucalypti and compare it with other species of the genus. We also deposit data from the internal transcribed spacer (ITS), small subunit (18S) ribosomal DNA (nuSSU),the RNA polymerase II 2nd largest subunit genes (RPB2), Translation Elongation Factor 1-α (TEF1-α) and β-Tubulin (Bt) genes from each strain in GenBank for future studies.

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Kurtzmanomyces shapotouensis sp. nov., an anamorphic, basidiomycetous yeast isolated from desert soil crusts

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.053058-0 ◽

2013 ◽

Vol 63 (Pt_10) ◽

pp. 3892-3895 ◽

Cited By ~ 8

Author(s):

Tao Zhang ◽

Rong-Liang Jia ◽

Yu-Qin Zhang ◽

Hong-Yu Liu ◽

Jing Su ◽

...

Keyword(s):

Novel Species ◽

Large Subunit ◽

Morphological Characteristics ◽

Western China ◽

Rrna Gene ◽

The Novel ◽

Internal Transcribed Spacer Region ◽

Soil Crusts ◽

D2 Domain ◽

Large Subunit Rrna

Two yeast strains isolated from soil crusts in the Shapotou region of Tengger Desert (north-western China) were grouped in the genus Kurtzmanomyces based on morphological characteristics. rRNA gene sequence analyses (including the D1/D2 domain of the large subunit rRNA gene and internal transcribed spacer region) indicated that these two strains represented a novel species of the genus Kurtzmanomyces, for which the name Kurtzmanomyces shapotouensis sp. nov. is proposed (type strain: CPCC 300020T = DSM 26579T = CBS 12707T). The MycoBank number of the novel species is MB 804959.

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Genetic Diversity and Aggressiveness of Fusarium spp. Isolated from Canola in Alberta, Canada

Plant Disease ◽

10.1094/pdis-01-13-0061-re ◽

2014 ◽

Vol 98 (6) ◽

pp. 727-738 ◽

Cited By ~ 11

Author(s):

Yongyan Chen ◽

Qixing Zhou ◽

Stephen E. Strelkov ◽

Sheau-Fang Hwang

Keyword(s):

Sequence Data ◽

Phylogenetic Analyses ◽

Geographic Origin ◽

Elongation Factor ◽

Morphological Characteristics ◽

Seedling Blight ◽

Fusarium Spp ◽

Translation Elongation ◽

Fusarium Seedling Blight ◽

Pathogenicity Tests

Canola (Brassica napus) is one of the most economically important oilseed crops in Canada. Fusarium seedling blight is a root disease with the potential to cause severe yield reductions in canola. Fusarium spp. are commonly isolated root pathogens from fields in Alberta. Fusarium infection can also cause root rot in adult plants. In this study, 128 isolates identified as Fusarium spp. were recovered from field soils in central Alberta and from the roots of diseased canola plants with typical Fusarium seedling blight symptoms. Six species of Fusarium were identified, with Fusarium acuminatum as the predominant species (57 of 128 isolates, 44.5%). Phylogenetic analyses based on the translation elongation factor 1-α and the internal transcribed spacer sequence data were used for evaluation of genetic variations, and also used for Fusarium spp. identification in combination with morphological characteristics and polymerase chain reaction-based analyses. Based on disease ratings in pathogenicity tests, six isolates of F. avenaceum showed high aggressiveness on canola. Also, the aggressiveness varied within all Fusarium spp. No correlation was observed between aggressiveness and the geographic origin of the isolates.

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