Zygotorulaspora dagestanica sp. nov., a novel ascomycetous yeast species associated with the Georgian honeysuckle (Lonicera iberica M. Bieb.)

During an investigation of the yeast communities associated with wild fruit shrubs in Dagestan (Caucasus, Russia), four fermenting ascospore-producing yeast strains were isolated from leaves of the Georgian honeysuckle (Lonicera iberica M. Bieb.) and from soil underneath this plant. Phylogenetic analyses based on concatenated sequences of the ITS region and D1/D2 domains of the large subunit rRNA gene and concatenated sequences of the ribosomal DNA cystron, RPB2 and TEF1 genes showed that the isolated strains represented a new species of the genus Zygotorulaspora. The new species was placed in the basal position to other species of the clade and close to Zygotorulaspora mrakii. Based on the results of phylogenetic analyses and the phenotypic characteristics of the four studied strains, a novel species is described, for which the name Zygotorulaspora dagestanica sp. nov. is proposed. The holotype is KBP Y-4591T, three metabolically inactive cryopreserved isotype cultures are DSM 100088, VKM Y-3060 and VKPM Y-4318. The MycoBank number is MB 838285.

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Nakazawaea Odontotermitis f.a., sp. nov., a Novel Yeast Isolated from the Gut of Odontotermes Horni in India.

10.21203/rs.3.rs-1113741/v1 ◽

2021 ◽

Author(s):

Snigdha Tiwari ◽

Bhaskar C. Behera ◽

Abhishek Baghela

Keyword(s):

New Species ◽

Type Strain ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Its Region ◽

Small Subunit ◽

Rrna Gene ◽

Nucleotide Substitutions ◽

Ascomycetous Yeast ◽

Lsu Rrna Gene

Abstract Three strains SMT1.3, SMT1.10, and SMT2.2, representing a novel asexual ascomycetous yeast species, were isolated from the gut of a termite Odontotermes horni in Maharashtra, India. Phylogenetic analyses of the LSU, ITS and SSU sequences revealed that they belonged to the genus Nakazawaea, with N. siamensis as the closest relative. The new species differed from the type strain of N. siamensis (DMKU-RK467T) by 1.93% nucleotide substitutions in the D1/D2 region of the large subunit (LSU) rRNA gene, 0.53% nucleotide substitutions in the small subunit (SSU) rRNA gene and 12.6% nucleotide substitutions in the internal transcribed spacer (ITS) region. Notable physiological differences were also observed between N. siamensis and the new species. Hence, the species Nakazawaea odontotermitis f.a., sp. nov. is proposed. The type strain is SMT1.3T (MTCC 13105 = NFCCI 5011). The GenBank accession numbers of the LSU and ITS and SSU sequences of Nakazawaea odontotermitis f.a., sp. nov. are MZ234240, MZ234239 and OK384663. The MycoBank number is MB 841926.

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Moelleriella sinensis sp. nov. (Clavicipitaceae, Ascomycota) from southeast China

Phytotaxa ◽

10.11646/phytotaxa.429.4.5 ◽

2020 ◽

Vol 429 (4) ◽

pp. 289-296

Author(s):

YU-XI CHEN ◽

QING-LIN XUE ◽

YONG-XIAO XIE ◽

NEMAT O. KEYHANI ◽

XIA-YU GUAN ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characterization ◽

Rrna Gene ◽

Distinct Lineage ◽

Spore Mass ◽

Large Subunit Rrna ◽

A New Species

Moelleriella sinensis, a fungal pathogen infecting whitefly nymphs (Hemiptera), is described and illustrated as a new species from Wu Yi Mountain in Fujian province, southeastern China. This species is characterized by producing a pale brown spore mass around whitish to pale yellow pulvinate stroma. In surveys of entomopathogenic fungal diversity, only the anamorphic state was found in collected stroma. Phylogenetic analyses using the nucleotide sequences derived from the nuclear ribosomal large subunit rRNA gene, RNA polymerase subunit 1, and translation elongation factor 1α, coupled to morphological characterization supports the placement of the isolate as a new species in a distinct lineage within Moelleriella.

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An Updated Global Species Diversity and Phylogeny in the Genus Wickerhamomyces with Addition of Two New Species from Thailand

Journal of Fungi ◽

10.3390/jof7110957 ◽

2021 ◽

Vol 7 (11) ◽

pp. 957

Author(s):

Supakorn Nundaeng ◽

Nakarin Suwannarach ◽

Savitree Limtong ◽

Surapong Khuna ◽

Jaturong Kumla ◽

...

Keyword(s):

New Species ◽

Yeast Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Its Region ◽

New Combination ◽

Full Description ◽

Ascomycetous Yeast ◽

Assam Tea ◽

Opportunistic Human Pathogen

Ascomycetous yeast species in the genus Wickerhamomyces (Saccharomycetales, Wickerhamomycetaceae) are isolated from various habitats and distributed throughout the world. Prior to this study, 35 species had been validly published and accepted into this genus. Beneficially, Wickerhamomyces species have been used in a number of biotechnologically applications of environment, food, beverage industries, biofuel, medicine and agriculture. However, in some studies, Wickerhamomyces species have been identified as an opportunistic human pathogen. Through an overview of diversity, taxonomy and recently published literature, we have updated a brief review of Wickerhamomyces. Moreover, two new Wickerhamomyces species were isolated from the soil samples of Assam tea (Camellia sinensis var. assamica) that were collected from plantations in northern Thailand. Herein, we have identified these species as W. lannaensis and W. nanensis. The identification of these species was based on phenotypic (morphological, biochemical and physiological characteristics) and molecular analyses. Phylogenetic analyses of a combination of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) of ribosomal DNA genes support that W. lannaensis and W. nanensis are distinct from other species within the genus Wickerhamomyces. A full description, illustrations and a phylogenetic tree showing the position of both new species have been provided. Accordingly, a new combination species, W. myanmarensis has been proposed based on the phylogenetic results. A new key for species identification is provided.

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Steinernema borjomiense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Georgia

Nematology ◽

10.1163/15685411-00003167 ◽

2018 ◽

Vol 20 (7) ◽

pp. 653-669 ◽

Cited By ~ 4

Author(s):

Oleg Gorgadze ◽

Elena Fanelli ◽

Manana Lortkhipanidze ◽

Alberto Troccoli ◽

Medea Burjanadze ◽

...

Keyword(s):

New Species ◽

18S Rrna ◽

Entomopathogenic Nematode ◽

Phylogenetic Analyses ◽

Tail Length ◽

Morphological Characters ◽

18S Rrna Gene ◽

The Body ◽

Rrna Gene ◽

A New Species

Summary A new species of entomopathogenic nematode, Steinernema borjomiense n. sp., was isolated from the body of the host insect, Oryctes nasicornis (Coleoptera: Scarabaeidae), in Georgia, in the territory of Borjomi-Kharagauli. Morphological characters indicate that the new species is closely related to species of the feltiae-group. The infective juveniles are characterised by the following morphological characters: body length of 879 (777-989) μm, distance between the head and excretory pore = 72 (62-80) μm, pharynx length = 132 (122-142) μm, tail length = 70 (60-80) μm, ratio a = 26.3 (23.0-29.3), H% = 45 (40-51), D% = 54 (47-59), E% = 102 (95-115), and lateral fields consisting of seven ridges (eight incisures) at mid-body. Steinernema borjomiense n. sp. was molecularly characterised by sequencing three ribosomal regions (the ITS, the D2-D3 expansion domains and the 18S rRNA gene) and the mitochondrial COI gene. Phylogenetic analyses revealed that S. borjomiense n. sp. differs from all other known species of Steinernema and is a member of the monticolum-group.

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A new species of Chlorociboria (Helotiales, Ascomycota) on herbaceous stems from China

Phytotaxa ◽

10.11646/phytotaxa.312.1.9 ◽

2017 ◽

Vol 312 (1) ◽

pp. 111

Author(s):

HUAN-DI ZHENG ◽

WEN-YING ZHUANG

Keyword(s):

New Species ◽

Dna Sequences ◽

Internal Transcribed Spacer ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Central China ◽

Nuclear Ribosomal Dna ◽

Light Blue ◽

Ectal Excipulum ◽

A New Species

A new species, namely Chlorociboria herbicola, is discovered on herbaceous stems in central China. Morphologically, the new fungus is distinctive by the combination of light blue-green apothecia, rectangular cells in ectal excipulum, and elongate-ellipsoidal ascospores with rounded ends. Phylogenetic analyses of the internal transcribed spacer and large subunit of nuclear ribosomal DNA sequences confirm its ascription in Chlorociboria and distinction from the known species of the genus.

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Morphological and molecular identification of a new species of Atraporiella (Polyporales, Basidiomycota) in China

Phytotaxa ◽

10.11646/phytotaxa.332.1.3 ◽

2017 ◽

Vol 332 (1) ◽

pp. 31 ◽

Cited By ~ 1

Author(s):

ZI-QIANG WU ◽

SHAN SHEN ◽

KAI-YUE LUO ◽

ZHENG-HUI WANG ◽

CHANG-LIN ZHAO

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Strong Support ◽

Molecular Data ◽

Fungal Species ◽

Monophyletic Lineage ◽

Inference Methods ◽

A New Species ◽

Molecular Characters

A new poroid wood-inhabiting fungal species, Atraporiella yunnanensis sp. nov., is proposed based on morphological and molecular characters. The species is characterized by cream pore surface when dry, which is easy to separate from substrate and very rapidly stained dark brown to black when bruised; hyphal system monomitic with generative hyphae hyaline to pale brown, thin-walled, unbranched, interwoven; slightly allantoid basidiospores, 2.2–3 × 0.8–1.5 µm. The internal transcribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA gene sequences of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analysis based on molecular data of ITS+nLSU sequences showed that Atraporiella yunnanensis belonged to the residual polyporoid clade, formed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and was closely related to A. neotropica, and then grouped with other related genera: Antrodiella, Pouzaroporia, Steccherinum and Xanthoporus. Both morphological and molecular characters confirmed the placement of the new species in Atraporiella.

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Description of a new toad of Megophrys Kuhl & Van Hasselt, 1822 (Amphibia: Anura: Megophryidae) from western Yunnan Province, China

Zootaxa ◽

10.11646/zootaxa.4942.3.3 ◽

2021 ◽

Vol 4942 (3) ◽

pp. 351-381

Author(s):

SHENGCHAO SHI ◽

DONGHUI LI ◽

WENBO ZHU ◽

WEN JIANG ◽

JIANPING JIANG ◽

...

Keyword(s):

Mitochondrial Dna ◽

New Species ◽

Yunnan Province ◽

Nuclear Dna ◽

Phylogenetic Analyses ◽

Rrna Gene ◽

Dorsal Skin ◽

Upper Eyelid ◽

Western Yunnan ◽

A New Species

A new species of genus Megophrys from Gaoligong Mountains, Yunnan Province, China is described. Phylogenetic analyses based on mitochondrial DNA and nuclear DNA all clustered the new species as an independent clade nested into the subgenus Panophrys. The smallest genetic distance based on 16S rRNA gene between the new species and its congeners was 3.0%. The new species could be identified from its congeners by a combination of following characters: moderate body size (SVL 31.0–34.8 mm in males); vomerine ridge weak, vomerine teeth absent; dorsal skin relatively smooth; tongue slightly notched behind; tympanum rounded and relatively large, 0.54 times of eye length; a horn-like tubercle on edge of each upper eyelid small; tibio-tarsal articulation reaches middle eye when leg stretched forward; finger tips rounded, not expanded to small pad; toes with narrow fringes and rudimentary webbing; ventral hindlimbs semitransparent purplish with greyish white pigments; ventral body scattered with distinct dark patches in the middle.

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Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

Parasites & Vectors ◽

10.1186/s13071-020-04430-6 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Yi-Fan Cao ◽

Hui-Xia Chen ◽

Yang Li ◽

Dang-Wei Zhou ◽

Shi-Long Chen ◽

...

Keyword(s):

New Species ◽

Ribosomal Dna ◽

Dna Sequences ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Small Subunit ◽

Sister Relationship ◽

Tibetan Antelope ◽

A New Species

Abstract Background The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites of the Tibetan antelope remains limited. Methods Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences, i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1), were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes. Results Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum n. sp. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions A new species of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum n. sp. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

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Lactarius guangdongensis sp. nov. (Russulaceae, Russulales), a species of Lactarius sect. Deliciosi growing with a vulnerable five-needle pine, Pinus kwangtungensis

Phytotaxa ◽

10.11646/phytotaxa.393.3.4 ◽

2019 ◽

Vol 393 (3) ◽

pp. 278

Author(s):

YING HAN ◽

CUN-ZHU LIANG ◽

PEI-GUI LIU ◽

JORINDE NUYTINCK ◽

XIANG-HUA WANG

Keyword(s):

New Species ◽

South China ◽

Ex Situ Conservation ◽

Phylogenetic Analyses ◽

Its Region ◽

Morphological Characters ◽

Ex Situ ◽

Dehydrogenase Gene ◽

A New Species

Lactarius sect. Deliciosi is a group of ectomycorrhizal mushrooms showing high host specificity with trees of Pinaceae and more rarely Fagales. A new species of this section, L. guangdongensis, is described from South China. This new species has the smallest basidiocarps (pileus 1–3 cm) in the section. The pure orange basidiocarps with faint greenish discoloration are similar to those of Abies-associated L. abieticola and L. laeticolor and Pinus-associated L. vividus. The subdistant lamellae and absence of pleuromacrocystidia can be used as valid characters for this species. It grows with Chinese national protected plant Pinus kwangtungensis and might be one of the very few species that form symbiosis with five-needle pines. Phylogenetic analyses of the ITS region and glyceraldehyde-3-phosphate dehydrogenase gene (gpd) show that L. guangdongensis clearly diversified from its relatives and look-alikes and probably represents an early diverging lineage in the section. Molecular and morphological characters provided in this study will be useful for in situ and ex situ conservation of P. kwangtungensis in the future.

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Morphological and phylogenetic analyses reveal a new species of Fistulina (Fistulinaceae, Agaricales) from Australia

Phytotaxa ◽

10.11646/phytotaxa.420.3.3 ◽

2019 ◽

Vol 420 (3) ◽

pp. 233-240

Author(s):

YI-FEI SUN ◽

SHUN LIU ◽

BAO-KAI CUI

Keyword(s):

New Species ◽

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Pore Surface ◽

Ribosomal Rna Gene ◽

Hyphal System ◽

Cotton Blue ◽

A New Species

A new species of Fistulina, F. tasmanica sp. nov., is described and illustrated from Tasmania in Australia. Fistulina tasmanica is distinguished by its pileate and fleshy basidiomata with rose pore surface, fibrous context, separated tubes with small pores (6–7 per mm), a monomitic hyphal system with only clamped generative hyphae, subellipsoid to ellipsoid basidiospores (5.4–6 × 3.4–4 μm) which are negative in Melzer’s reagent and cotton blue. Phylogenetic analyses inferred from the internal transcribed spacer regions (ITS) and the large subunit of nuclear ribosomal RNA gene (nLSU) sequences also confirmed that F. tasmanica is a new member within Fistulina.

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