Thyroid-Stimulating Hormone Stimulation Tests in the Bottlenose Dolphin (Tursiops truncatus)

Stimulation of the thyroid with thyroid-stimulating hormone (TSH) is a potentially useful diagnostic of thyroid dysfunction, but little is known about the response of the thyroid to TSH stimulation in bottlenose dolphins (Tursiops truncatus). To better characterize the response of the dolphin thyroid to TSH stimulation, five adult dolphins participated in a TSH stimulation study. Dolphins voluntarily beached onto a padded mat and were given a 1.5 mg intramuscular injection of human recombinant TSH. Blood samples collected the day prior, at multiple intervals the day of, and daily for three days after the injection were analyzed via radioimmunoassay for free and total triiodothyronine (fT3 and tT3), and free and total thyroxine (fT4 and tT4). Significant increases in circulating fT3, fT4, and tT4 were observed with peaks occurring for all hormones the day after the TSH injection; maximal increases were 44%, 47%, and 23% for each hormone, respectively. Temporal patterns in the hormones potentially reflected feedback mechanisms countering the surge in fT3 following stimulation. Though recombinant human TSH was effective at stimulating hormone release, it is likely that use of dolphin or dolphin-derived TSH would enhance the clinical utility of the stimulation test, as would the development of antibodies specific to dolphin TSH.

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Establishment of reference intervals for serum thyroid-stimulating hormone, free and total thyroxine, and free and total triiodothyronine for the Beckman Coulter DxI-800 analyzers by indirect method using data obtained from Chinese population in Zhejiang

Journal of Clinical Laboratory Analysis ◽

10.1002/jcla.22069 ◽

2016 ◽

Vol 31 (4) ◽

pp. e22069 ◽

Cited By ~ 6

Author(s):

Yan Wang ◽

Yu-xia Zhang ◽

Yong-lie Zhou ◽

Jun Xia

Keyword(s):

Chinese Population ◽

Indirect Method ◽

Reference Intervals ◽

Thyroid Stimulating Hormone ◽

Total Triiodothyronine ◽

Total Thyroxine ◽

Using Data ◽

Stimulating Hormone

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Diffuse Nontoxic Goiter in Children and Its Impact on Dental Pathology

Galician Medical Journal ◽

10.21802/gmj.2018.1.7 ◽

2018 ◽

Vol 25 (1) ◽

Author(s):

Oksana Hodovanets ◽

Tetiana Kitsak ◽

Oleksandr Vitkovskyj

Keyword(s):

Thyroid Stimulating Hormone ◽

World Health ◽

Thyroid Status ◽

Dental Tissues ◽

Periodontal Tissues ◽

Nontoxic Goiter ◽

Dental Pathology ◽

Total Triiodothyronine ◽

Total Thyroxine ◽

Stimulating Hormone

The objective of the research was to assess the thyroid status of children with diffuse nontoxic goiter and its effect on dental pathology depending on age.Materials and methods. Clinical observation of 226 children at the age of 12-15 years was conducted. To analyze their thyroid status, serum levels of total thyroxine, free thyroxine, total triiodothyronine, and thyroid stimulating hormone were determined using enzyme immunoassay. The following thyroid indices were calculated for the integral estimation of the functional state of the pituitary-thyroid system: the peripheral inversion index (total triiodothyronine/total thyroxine), the integral index (total triiodothyronine + total thyroxine/thyroid stimulating hormone) and the indices of thyroid stimulating hormone/total triiodothyronine and thyroid stimulating hormone/total thyroxine. Their dental status was determined by means of standard indices recommended by the World Health Organization.Conclusions. In children with euthyroid enlargement of the thyroid gland, there were detected changes in the thyroid status within the reference range. According to the direction of changes in the most indices, dysthyroidism is characterized by the reduced thyroid function that can affect metabolic processes in the body, including the dentofacial system, as evidenced by significantly worse indices of the intensity of damage to hard dental tissues and periodontal tissues in children with diffuse nontoxic goiter.

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Abnormal endocrine tests in a hemodialysis patient.

Journal of the American Society of Nephrology ◽

10.1681/asn.v4101754 ◽

1994 ◽

Vol 4 (10) ◽

pp. 1754-1759

Author(s):

L A Hochstetler ◽

M J Flanigan ◽

V S Lim

Keyword(s):

Hemodialysis Patient ◽

Thyroid Stimulating Hormone ◽

Thyrotropin Releasing Hormone ◽

Serum Prolactin ◽

Thyroid Function Tests ◽

Releasing Hormone ◽

Total Triiodothyronine ◽

Total Thyroxine ◽

Prolactin Response ◽

Stimulating Hormone

A home hemodialysis patient with abnormal thyroid function tests and hyperprolactinemia is presented. Abnormal thyroid tests included low total triiodothyronine and low total thyroxine and free thyroxine; the latter was documented by the use of both RIA and direct dialysis equilibrium techniques. Serum thyroid-stimulating hormone was normal, and the thyroid-stimulating hormone response to thyrotropin-releasing hormone stimulation was in the low-normal range. Serum prolactin was elevated to more than twice the normal level, and the prolactin response to thyrotropin-releasing hormone was also blunted. The interpretation, pathogenesis, physiologic significance, and management of these abnormalities are discussed.

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The relationships between blood lead levels and serum thyroid stimulating hormone and total thyroxine in the third National Health and Nutrition Examination Survey

Journal of Trace Elements in Medicine and Biology ◽

10.1016/j.jtemb.2018.10.010 ◽

2019 ◽

Vol 51 ◽

pp. 130-137 ◽

Cited By ~ 1

Author(s):

Edward F. Krieg

Keyword(s):

National Health ◽

Blood Lead ◽

Thyroid Stimulating Hormone ◽

Blood Lead Levels ◽

Nutrition Examination Survey ◽

The Third ◽

Health And Nutrition ◽

Total Thyroxine ◽

Lead Levels ◽

Stimulating Hormone

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Inappropriate thyroid-stimulating hormone release in the elderly?

La Ricerca in Clinica e in Laboratorio ◽

10.1007/bf03029838 ◽

1985 ◽

Vol 15 (2) ◽

Author(s):

Antonio E. Pontiroli ◽

Miriam Alberetto ◽

Guido Pozza

Keyword(s):

The Elderly ◽

Thyroid Stimulating Hormone ◽

Hormone Release ◽

Stimulating Hormone

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Lacking evidence for release of thyroid hormones from circulating thyroglobulin during subtotal thyroidectomy

Acta Endocrinologica ◽

10.1530/acta.0.1170219 ◽

1988 ◽

Vol 117 (2) ◽

pp. 219-224 ◽

Cited By ~ 1

Author(s):

J. Date ◽

M. Blichert-Toft ◽

U. Feldt-Rasmussen ◽

V. Haas

Keyword(s):

Thyroid Hormones ◽

Mean Value ◽

Thyroid Stimulating Hormone ◽

Mean Values ◽

Thyroid Resection ◽

Free Thyroxine Index ◽

Total Triiodothyronine ◽

Total Thyroxine ◽

The Mean ◽

Peak Value

Abstract. The effect of subtotal thyroid resection for thyrotoxicosis on concentrations of serum thyroid hormones and thyroglobulin (Tg), was determined in 10 patients during operation and the subsequent 18 days. Mean serum Tg responded drastically, increasing from a pre-operative value of 0.30 nmol/l to a peak value of approximately 26 nmol/l during operation followed by a gradual decline to levels lower than before surgery on day 18. Mean serum total thyroxine was 114 nmol/l pre-operatively and free thyroxine index (FT4I) 105 units. Both fluctuated only slightly during operation. Postsurgically, the mean values decreased to below 50% of the pre-operative level. Mean serum total triiodothyronine (TT3) was 1.46 nmol/l pre-operatively. It decreased during operation, reaching a nadir of 0.55 nmol/l on day 2, whereafter the concentration increased slightly. Mean serum reverse T3 (rT3) was 0.45 nmol/l pre-operatively, increased 62% during surgery, and decreased postsurgically. The mean value of serum thyroid stimulating hormone (TSH) was 0.61 mU/l pre-operatively and remained below 1 mU/l during and after operation, but from day 10 concentration began to rise steadily. It is concluded that the vast release of Tg during thyroid resection did not contribute to the concentration of serum T4 to an extent of clinical relevance.

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SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5Kγ Activity through Deacetylation of Specific Lysine Residues in Mammals

PLoS ONE ◽

10.1371/journal.pone.0011755 ◽

2010 ◽

Vol 5 (7) ◽

pp. e11755 ◽

Cited By ~ 28

Author(s):

Sayaka Akieda-Asai ◽

Nobuhiro Zaima ◽

Koji Ikegami ◽

Tomoaki Kahyo ◽

Ikuko Yao ◽

...

Keyword(s):

Thyroid Stimulating Hormone ◽

Hormone Release ◽

Lysine Residues ◽

Stimulating Hormone

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636: Isolated hypothyroxinemia in early gestation reflects low total thyroxine levels, while isolated high thyroid-stimulating hormone (TSH) does not

American Journal of Obstetrics and Gynecology ◽

10.1016/j.ajog.2008.09.666 ◽

2008 ◽

Vol 199 (6) ◽

pp. S183

Author(s):

Fionnuala Breathnach ◽

Cathy Rooney ◽

Achim Treumann ◽

Jennifer Donnelly ◽

Sharon Cooley ◽

...

Keyword(s):

Thyroid Stimulating Hormone ◽

Early Gestation ◽

Total Thyroxine ◽

Stimulating Hormone

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Marked Biological Variance in Endocrine and Biochemical Markers in Childhood: Establishment of Pediatric Reference Intervals Using Healthy Community Children from the CALIPER Cohort

Clinical Chemistry ◽

10.1373/clinchem.2013.204222 ◽

2013 ◽

Vol 59 (9) ◽

pp. 1393-1405 ◽

Cited By ~ 80

Author(s):

Dana Bailey ◽

David Colantonio ◽

Lianna Kyriakopoulou ◽

Ashley H Cohen ◽

Man Khun Chan ◽

...

Keyword(s):

Reference Values ◽

Biochemical Markers ◽

Troponin I ◽

Reference Intervals ◽

Thyroid Stimulating Hormone ◽

Healthy Children ◽

Free Triiodothyronine ◽

Laboratory Test Results ◽

Total Triiodothyronine ◽

Total Thyroxine

BACKGROUND Reference intervals are indispensable in evaluating laboratory test results; however, appropriately partitioned pediatric reference values are not readily available. The Canadian Laboratory Initiative for Pediatric Reference Intervals (CALIPER) program is aimed at establishing the influence of age, sex, ethnicity, and body mass index on biochemical markers and developing a comprehensive database of pediatric reference intervals using an a posteriori approach. METHODS A total of 1482 samples were collected from ethnically diverse healthy children ages 2 days to 18 years and analyzed on the Abbott ARCHITECT i2000. Following the CLSI C28-A3 guidelines, age- and sex-specific partitioning was determined for each analyte. Nonparametric and robust methods were used to establish the 2.5th and 97.5th percentiles for the reference intervals as well as the 90% CIs. RESULTS New pediatric reference intervals were generated for 14 biomarkers, including α-fetoprotein, cobalamin (vitamin B12), folate, homocysteine, ferritin, cortisol, troponin I, 25(OH)-vitamin D [25(OH)D], intact parathyroid hormone (iPTH), thyroid-stimulating hormone, total thyroxine (TT4), total triiodothyronine (TT3), free thyroxine (FT4), and free triiodothyronine. The influence of ethnicity on reference values was also examined, and statistically significant differences were found between ethnic groups for FT4, TT3, TT4, cobalamin, ferritin, iPTH, and 25(OH)D. CONCLUSIONS This study establishes comprehensive pediatric reference intervals for several common endocrine and immunochemical biomarkers obtained in a large cohort of healthy children. The new database will be of global benefit, ensuring appropriate interpretation of pediatric disease biomarkers, but will need further validation for specific immunoassay platforms and in local populations as recommended by the CLSI.

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