scholarly journals Estimation of the Uncertainties Related to the Measurement of the Size and Quantities of Individual Silver Nanoparticles in Confectionery

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2677 ◽  
Author(s):  
Nadia Waegeneers ◽  
Sandra De Vos ◽  
Eveline Verleysen ◽  
Ann Ruttens ◽  
Jan Mast
Keyword(s):  
Silver Nanoparticles ◽  
Sample Preparation ◽  
Measurement Uncertainty ◽  
Inductively Coupled Plasma ◽  
Food Additives ◽  
Data Interpretation ◽  
Background Concentration ◽  
Inductively Coupled ◽  
Validation Parameters ◽  
Plasma Mass Spectrometry

E174 (silver) is a food additive that may contain silver nanoparticles (AgNP). Validated methods are needed to size and quantify these particles in a regulatory context. However, no validations have yet been performed with food additives or real samples containing food additives requiring a sample preparation step prior to analysis. A single-particle inductively coupled plasma mass spectrometry (spICP-MS) method was developed and validated for sizing and quantifying the fraction of AgNP in E174 and in products containing E174, and associated uncertainties related to sample preparation, analysis and data interpretation were unraveled. The expanded measurement uncertainty for AgNP sizing was calculated to be 16% in E174-containing food products and increased up to 23% in E174 itself. The E174 food additives showed a large silver background concentration combined with a relatively low number of nanoparticles, making data interpretation more challenging than in the products. The standard uncertainties related to sample preparation, analysis, and challenging data interpretation were respectively 4.7%, 6.5%, and 6.0% for triplicate performances. For a single replicate sample, the uncertainty related to sample preparation increased to 6.8%. The expanded measurement uncertainty related to the concentration determination was 25–45% in these complex samples, without a clear distinction between additives and products. Overall, the validation parameters obtained for spICP-MS seem to be fit for the purpose of characterizing AgNP in E174 or E174-containing products.

scholarly journals Quantification of silver nanoparticles taken up by single cells using inductively coupled plasma mass spectrometry in the single cell measurement mode

2018 ◽  
Vol 33 (7) ◽  
pp. 1256-1263 ◽  
Author(s):  
Ana López-Serrano Oliver ◽  
Sabine Baumgart ◽  
Wolfram Bremser ◽  
Sabine Flemig ◽  
Doreen Wittke ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Silver Nanoparticles ◽  
Cell Surface ◽  
Inductively Coupled Plasma ◽  
Single Cells ◽  
Analytical Methodology ◽  
Inductively Coupled ◽  
Cell Measurement ◽  
Measurement Mode ◽  
Plasma Mass Spectrometry

A promising analytical methodology is proposed to study nanoparticle-cell interactions providing information of the number of NPs internalized by cells or externally bound to the cell surface.

scholarly journals Puparial Cases as Toxicological Indicators: Bioaccumulation of Cadmium and Thallium in the Forensically Important Blowfly Lucilia sericata

2020 ◽  
Vol 8 ◽  
Author(s):  
Julita Malejko ◽  
Krzysztof Deoniziak ◽  
Marlena Tomczuk ◽  
Joanna Długokencka ◽  
Beata Godlewska-Żyłkiewicz
Keyword(s):  
Inductively Coupled Plasma ◽  
Certified Reference Materials ◽  
Larval Growth ◽  
Lucilia Sericata ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Validation Parameters ◽  
Negative Effect ◽  
Plasma Mass Spectrometry ◽  
Feeding Substrate

In this study, we present entomotoxicological data on the accumulation of cadmium and thallium in a forensically important blowfly, Lucilia sericata, and evaluate the reliability and utility of such information as toxicological evidence for poisoning as a cause of death. We observed that Cd and Tl content in different growing stages of L. sericata (larvae, puparial cases, and adults) was increasing with increasing metal concentration in the feeding substrate, namely metal-enriched liver. However, patterns of accumulation differed between the two metals investigated, showing a linear relationship for Cd and a saturable pattern for Tl. For cadmium, the highest bioaccumulation factor (BAF) was found in the larval stage (in the range of 0.20–0.25), while for thallium, puparial cases accumulated more metal than the other stages tested (BAF in the range of 0.24–0.42). Thallium was also observed to have a negative effect on larval growth, resulting in lower weight and smaller puparial size. With this study, we update the information on the bioaccumulation of cadmium in forensically important blowflies and provide the first report on the bioaccumulation of thallium as well as its developmental impact in blowflies. Specifically, our results suggest that analysis of puparial cases could yield useful information for entomotoxicological investigations. The content of Cd and Tl in larvae, puparial cases, and adults of L. sericata was determined by inductively coupled plasma mass spectrometry (ICP-MS). The validation parameters of the method such as sensitivity, detection limits, quantification limits, precision, and accuracy were evaluated. The method detection limit (MDL) for all types of samples was in the range of 1.6–3.4 ng g−1 for Cd and 0.034–0.15 ng g−1 for Tl, and the accuracy of the method was confirmed by a high recovery of metals from certified reference materials (91.3% for Cd and 94.3% for Tl).

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