scholarly journals Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders

Materials ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 447
Author(s):  
Asaf Olshinka ◽  
Dean Ad-El ◽  
Elena Didkovski ◽  
Shirel Weiss ◽  
Rinat Ankri ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
False Positive Rate ◽  
False Negative ◽  
False Negative Rate ◽  
Residual Tumor ◽  
Cutaneous Squamous Cell Carcinoma ◽  
Diffusion Reflection

Diffusion reflectance spectroscopy measurements targeted with gold nanoparticles (GNPs) can identify residual cutaneous squamous cell carcinoma (SCC) in excision borders. Human SCC specimens were stained with hematoxylin and eosin to identify tumor borders, and reflected onto an unstained deparaffinized section. Diffusion reflection of three sites (normal and SCC) were measured before and after GNPs targeting. Hyperspectral imaging showed a mean of 2.5 sites with tumor per specimen and 1.2 tumor-free (p < 0.05, t-test). GNPs were detected in 25/30 tumor sites (sensitivity 83.3%, false-negative rate 16.6%) and 12/30 non-tumor sites (specificity 60%, false-positive rate 40%). This study verifies the use of nanotechnology in identifying SCC tumor margins. Diffusion reflection scanning has high sensitivity for detecting the residual tumor.

Dynamic sentinel lymph node biopsy in patients with invasive squamous cell carcinoma of the penis: A prospective study of the outcome of 500 inguinal basins assessed in a single institution.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. 4640-4640
Author(s):  
Wayne Lam ◽  
Hussain M Alnajjar ◽  
Susannah La-Touche ◽  
Matthew Perry ◽  
Catherine M Corbishley ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
False Negative ◽  
False Negative Rate ◽  
Lymph Node Biopsy ◽  
Invasive Squamous Cell Carcinoma ◽  
Term Outcome ◽  
Long Term Outcome ◽  
Node Biopsy

4640 Background: Dynamic sentinel node biopsy (DSNB) in combination with ultrasound scan (USS) has been the technique of choice at our centre since 2004 for the assessment of non palpable inguinal lymph nodes in patients with squamous cell carcinoma of the penis (SCCp). Sensitivity/false-negative rates may vary depending on whether results are reported per patient or per node basin and with and without USS. The purpose of this study was to determine the long-term outcome of DSNB and ultrasound-guided fine needle aspiration cytology (FNAC) in our cohort of newly diagnosed patients and to analyse any variation in sensitivity of the procedure. Methods: A prospective cohort study over 6 years (2004 to 2010). Inclusion criteria: New diagnosis SCCp, T1G2 or greater definitive histology, non-palpable nodes in inguinal basin. Exclusion: patient with persistent/untreated local disease. Sensitivity of the procedure was calculated, per node basin, per patient, DSNB alone, USS/DSNB combined. Minimum follow up 12 months. Results: 500 inguinal basins in 264 patients underwent USS+/-FNAC and DSNB. 70 (14%) positive inguinal basins in 57(22%) patients were identified. 9 (2%) inguinal basins had no tracer uptake. 2 inguinal basins were confirmed false negative at 8 and 12 months. 2 inguinal basins had positive USS+FNAC and negative DSNB. Overall sensitivity of the technique is reported in the table. Conclusions: DSNB in combination with USS has excellent performance characteristics to stage patients with clinically node-negative penile cancer with a 3% false negative rate. USS improves performance by 4% over DSNB alone. There is no difference in performance of the combined technique if it is reported per node basin or per patient. [Table: see text]

PD-L1 Expression in Esophageal Squamous Cell Carcinoma: A Comparative Analysis of 3 Different Antibodies

2020 ◽  
Author(s):  
Lei Guo ◽  
Ying Ji ◽  
Wei Guo ◽  
Huayu He ◽  
Peng Song ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Esophageal Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
False Negative ◽  
False Negative Rate ◽  
High Sensitivity ◽  
Tissue Microarrays ◽  
Programmed Cell Death 1 ◽  
High Concordance

Abstract Background: Several studies have assessed the comparability of various immunohistochemical assays for programmed cell death 1 ligand 1 (PD-L1) expression in different kinds of tumours. However, there is a lack of relevant research on the detection efficacy of different PD-L1 clones in esophageal squamous cell carcinoma (ESCC). This study was performed to compare the PD-L1 expression results of three PD-L1 antibodies to assess their effectiveness.Methods: Three hundred and twenty-four cases of ESCC tissues in our medical institution were used to prepare tissue microarrays (TMAs). TMAs were stained with three validated assays (Ventana’s SP263 and Dako’s 28-8 and 22C3). Then, pathologists scored each tissue according to PD-L1 staining.Results: We found very high concordance when comparing 28-8 with SP263 at both the 1% (95.7%) and 50% (99.1%) cutoffs. Low overall concordance was found between 28-8 and 22C3 at the 1% cutoff (85.5%). Moreover, sensitivities of 91.2% and 87.5% were found for 28-8 compared with SP263 at the 1% and 50% cutoffs, respectively. Sensitivities of only 75.6% and 50% were found for 28-8 compared with 22C3 at the 1% and 50% cutoffs, respectively, which may result in more false negatives.Conclusions: We demonstrate that the PD-L1 SP263 shows high levels of overall agreement with 28-8 and relatively high sensitivity, with a low false negative rate. Thus, SP263 seems to be a reliable assay for PD-L1 testing in ESCC when considering pembrolizumab as immunotherapy.

Two-Center Evaluation of Dynamic Sentinel Node Biopsy for Squamous Cell Carcinoma of the Penis

2009 ◽  
Vol 27 (20) ◽  
pp. 3325-3329 ◽  
Author(s):  
Joost A.P. Leijte ◽  
Ben Hughes ◽  
Niels M. Graafland ◽  
Bin K. Kroon ◽  
Renato A. Valdés Olmos ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Learning Curve ◽  
Sentinel Node ◽  
Cell Carcinoma ◽  
Sentinel Node Biopsy ◽  
Squamous Cell ◽  
False Negative ◽  
False Negative Rate ◽  
Node Negative ◽  
Node Biopsy

Purpose Sentinel node biopsy is used to evaluate the nodal status of patients with clinically node-negative penile carcinoma. Its use is not widespread, and the majority of patients with clinically node-negative disease undergo an elective inguinal lymph node dissection. Reservations about the use of sentinel node biopsy include the fact that most current results come from one institution and the supposedly long learning curve associated with the procedure. The purpose of this study was to address these issues by analyzing results from two centers and by evaluating the learning curve. Patients and Methods All patients undergoing sentinel node biopsy for penile carcinoma at two centers were included. The sentinel node identification rate, false-negative rate, and morbidity of the procedure were calculated. Results from the first 30 procedures were assessed for a potential learning curve. Results A total of 323 patients with penile squamous cell carcinoma, which included 611 clinically node-negative groins, were scheduled for sentinel node biopsy. A sentinel node was found in 572 of the 592 groins (97%) that proceeded to sentinel node biopsy. In 79 groins, a sentinel node was positive for tumor. Six inguinal node recurrences occurred after a negative sentinel node procedure, all within 15 months after sentinel node biopsy. The combined false-negative rate was 7%. Complications occurred in 4.7% of explored groins. None of the false-negative procedures occurred in the initial 30 procedures. Conclusion Sentinel node biopsy is a suitable procedure to stage clinically node-negative penile cancer, and it has a low complication rate. No learning curve was demonstrated in this study.

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