scholarly journals Fabrication of Bioprobe Self-Assembled on Au–Te Nanoworm Structure for SERS Biosensor

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3234
Author(s):  
Soo Min Kim ◽  
Taek Lee ◽  
Yeong-Gyu Gil ◽  
Ga Hyeon Kim ◽  
Chulhwan Park ◽  
...  
Keyword(s):  
Surface Enhanced Raman Spectroscopy ◽  
Galvanic Replacement ◽  
Ambient Conditions ◽  
Force Microscopy ◽  
Atomic Force ◽  
Surface Enhanced ◽  
Immunodeficiency Virus ◽  
Surface Enhanced Raman ◽  
Immobilized Substrate ◽  
Interconnected Structure

In the present study, we propose a novel biosensor platform using a gold-tellurium (Au–Te) nanoworm structure through surface-enhanced Raman spectroscopy (SERS). Au–Tenanoworm was synthesized by spontaneous galvanic replacement of sacrificial Te nanorods templated with Au (III) cations under ambient conditions. The fabricated Au–Te nanoworm exhibited an interconnected structure of small spherical nanoparticles and was found to be effective at enhancing Raman scattering. The Au–Te nanoworm-immobilized substrate exhibited the ability to detect thyroxine using an aptamer-tagged DNA three-way junction (3WJ) and glycoprotein 120 (GP120) human immunodeficiency virus (HIV) using an antibody. The modified substrates were investigated by scanning electron microscopy and atomic force microscopy (AFM). The optimal Au–Te nanoworm concentration and immobilization time for the thyroxine biosensor platform were further determined by SERS experimentation. Thus, the present study showed that the Au–Te nanoworm structure could be applied to various biosensor platforms.

Surface-Enhanced Raman Scattering and Atomic Force Microscopy of Brass Electrodes in Sulfuric Acid Solution Containing Benzotriazole and Chloride Ion

1993 ◽  
Vol 47 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Joel C. Rubim ◽  
Jae-Ho Kim ◽  
Eric Henderson ◽  
Therese M. Cotton
Keyword(s):  
Atomic Force Microscopy ◽  
Sulfuric Acid ◽  
Raman Scattering ◽  
Acid Solution ◽  
Chloride Ion ◽  
Force Microscopy ◽  
Atomic Force ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Three different methods were used to roughen brass (Cu/Zn = 67/33) electrodes in 0.5 M H2SO4 containing 1.0 mM benzotriazole (BTAH): (1) polarization at +0.05 V vs. saturated calomel for 5 min; (2) immersion in the above solution for six hours; and (3) oxidation-reduction cycling in the presence of chloride ion. The surfaces prepared by the first two methods exhibited surface-enhanced Raman scattering (SERS) spectra of the polymeric complex [Cu(I)BTA] n. The SERS spectrum obtained from electrodes prepared by the third method is very similar to that of [CU(I)C1BTAH]4. Examination of the electrodes by atomic force microscopy (AFM) showed that a large number of grain boundary sites are formed by the roughening processes. This effect is attributed to the loss of zinc, which occurs during corrosion of the mirror-like, polished brass electrode surface in the sulfuric acid solution.

scholarly journals Raman spectroscopy methods for detecting and imaging supported lipid bilayers

2010 ◽  
Vol 24 (1-2) ◽  
pp. 113-117 ◽  
Author(s):  
Claire S. Sweetenham ◽  
Ioan Notingher
Keyword(s):  
Raman Spectroscopy ◽  
Lipid Bilayers ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Microspectroscopy ◽  
Supported Lipid Bilayers ◽  
Chemical Information ◽  
Spatial Features ◽  
Atomic Force ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

We have developed a Raman microspectroscopy system optimised for studying supported lipid bilayers (SLB). This system combines the benefits of Raman spectroscopy with the high spatial resolution of confocal microscopy. Furthermore, the additional incorporation of an atomic force microscope (AFM) makes it possible to directly correlate chemical information with spatial features of samples at the nanoscale. We focus on the limits of this system for detecting a single SLB and imaging its microdomains, and employ surface-enhanced Raman spectroscopy (SERS) to improve the sensitivity achieved with Raman microspectroscopy.

scholarly journals Micro- and Nanoscale Spectroscopic Investigations of Threonine Influence on the Corrosion Process of the Modified Fe Surface by Cu Nanoparticles

Materials ◽  
2020 ◽  
Vol 13 (20) ◽  
pp. 4482
Author(s):  
Dominika Święch ◽  
Czesława Paluszkiewicz ◽  
Natalia Piergies ◽  
Ewa Pięta ◽  
Kamila Kollbek ◽  
...  
Keyword(s):  
Metal Surface ◽  
Vibrational Analysis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Cu Nanoparticles ◽  
Force Microscopy ◽  
Carboxylate Groups ◽  
Protonated Amine ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Adsorption Geometry

The work presents a comprehensive vibrational analysis of the process of adsorption of threonine (Thr) onto an Fe surface with deposited Cu nanoparticles (NPs) (of about 4–5 nm in size) in a corrosive environment. The application of surface-enhanced Raman spectroscopy (SERS) and surface-enhanced infrared absorption spectroscopy (SEIRA) provides the opportunity for detailed description of adsorption geometry of amino acid onto a metal surface. The combination of conventional infrared spectroscopy (IR) with atomic force microscopy (AFM) resulted in a nano-SEIRA technique which made it possible to provide a precise description of adsorbate binding to the metal surface. The studies presented confirmed that there is a very good correlation between the spectra recorded by the SERS, SEIRA, and nano-SEIRA techniques. Threonine significantly influenced the process of corrosion of the investigated surface due to the existing strong interaction between the protonated amine and carboxylate groups and the CuNPs deposited onto the Fe surface. In addition, the application of two polarization modulations (s and p) in nano-SEIRA allows subtle changes to be observed in the molecule geometry upon adsorption, with the carboxylate group of Thr being almost horizontally oriented onto the metal surface; whereas the amine group that contains nitrogen is oriented perpendicular to this surface.

Sign in / Sign up

Export Citation Format

Share Document