scholarly journals Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro

Materials ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 221
Author(s):  
Avi Meirowitz ◽  
Arkadi Rahmanov ◽  
Eti Shlomo ◽  
Helena Zelikman ◽  
Eran Dolev ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Candida Albicans ◽  
3D Printing ◽  
Oral Candidiasis ◽  
Acrylic Resin ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Heat Curing ◽  
Vitro Effect

Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.

scholarly journals Surface Characteristics of Milled and 3D Printed Denture Base Materials Following Polishing and Coating: An In-Vitro Study

Materials ◽  
2020 ◽  
Vol 13 (15) ◽  
pp. 3305
Author(s):  
Pablo Kraemer Fernandez ◽  
Alexey Unkovskiy ◽  
Viola Benkendorff ◽  
Andrea Klink ◽  
Sebastian Spintzyk
Keyword(s):  
Surface Roughness ◽  
3D Printing ◽  
In Vitro Study ◽  
Surface Characteristics ◽  
Denture Base ◽  
Liquid Resin ◽  
Base Materials ◽  
Superior Surface ◽  
3D Printed

(1) Background: To date, no information on the polishability of milled and 3D-printed complete denture bases has been provided, which is relevant in terms of plaque accumulation. (2) Methods: three groups (n = 30) were manufactured using the cold-polymerization polymethilmethacrilate, milling (SM) and 3D printing (AM). 10 specimens of each group were left untreated (reference). 10 more specimens were pre-polished (intermediate polishing) and 10 final specimens were highgloss polished. An additional 20 specimens were 3D printed and coated with the liquid resin (coated), 10 of which were additionally polished (coated + polished). For each group Ra and Rz values, gloss value and REM images were obtained. (3). The “highgloss-polished” specimens showed statistically lower Ra and Rz values in the SM, followed by AM and conventional groups. In the AM group statistically lower surfaces roughness was revealed for highgloss-polished, “coated + polished”, and “coated” specimens, respectively. (4) Conclusions: The milled specimens demonstrated superiors surface characteristics than 3D printed and conventionally produced after polishing. The polished specimens demonstrated superior surface characteristics over coated specimens. However, the surface roughness by both polished and coated specimens was within the clinically relevant threshold of 0.2 µm.

Effect of human whole saliva on the in vitro adhesion of Candida albicans to a denture base acrylic resin: a focus on collection and preparation of saliva samples

2012 ◽  
Vol 4 (4) ◽  
pp. 225-228 ◽  
Author(s):  
Camila A. Zamperini ◽  
Patricia C. S. Schiavinato ◽  
Ana C. Pavarina ◽  
Eunice T. Giampaolo ◽  
Carlos E. Vergani ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Acrylic Resin ◽  
Denture Base ◽  
Whole Saliva ◽  
In Vitro Adhesion

Surface Adherence of Candida albicans to Different Polymethyl Methacrylate Resin Denture Base Materials

2011 ◽  
Vol 2 (3) ◽  
pp. 237-242
Author(s):  
Achut R Devarhubli ◽  
VK Subbarao ◽  
NP Patil
Keyword(s):  
Candida Albicans ◽  
Polymethyl Methacrylate ◽  
Cobalt Alloy ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Base Materials ◽  
Normal Individuals ◽  
Cobalt Chrome Alloy ◽  
Cobalt Chrome

ABSTRACT Over the years researchers have reported on the frequency and distribution of yeast in the oropharynx of apparently normal individuals and those with systemic or mycotic diseases wearing dentures. Few workers have taken into consideration the role played by the legion of denture base materials on the occurrence and progression of denture stomatitis. Hence this study was undertaken with the objective of evaluating the in vitro adherence of Candida albicans to different denture base materials namely reinforced polymethyl methacrylate, Nonreinforced polymethyl methacrylate and Cobalt chrome alloy of which chrome cobalt alloy showed less adherence to candidal cells compared to the two polymethyl methacrylate resins.

scholarly journals Adherence in vitro of Candida albicans to plasma treated acrylic resin. Effect of plasma parameters, surface roughness and salivary pellicle

2010 ◽  
Vol 55 (10) ◽  
pp. 763-770 ◽  
Author(s):  
Camila Andrade Zamperini ◽  
Ana Lucia Machado ◽  
Carlos Eduardo Vergani ◽  
Ana Claudia Pavarina ◽  
Eunice Terezinha Giampaolo ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Candida Albicans ◽  
Acrylic Resin ◽  
Plasma Parameters ◽  
Salivary Pellicle

scholarly journals Chitosan 2% Effect on Prohibiting the Growth of Candida Albicans on Heatcured Acrylic Resin

2017 ◽  
Vol 6 (2) ◽  
pp. 17
Author(s):  
Angela Evelyna ◽  
Dahlia Sutanto ◽  
Elizabeth Tiffany
Keyword(s):  
Candida Albicans ◽  
Statistical Analysis ◽  
Natural Compound ◽  
Agar Medium ◽  
Acrylic Resin ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Base Materials ◽  
Oral Tissue ◽  
The Difference

Maintaining acrylic denture hygiene is a necessary to prohibit the growth of several oral fungi such as Candida albicans. Candida albicans are the main etiological microorganism of denture stomatitis. One of the ways to clean denture is by immersion on synthetic denture cleaner liquid such as oxygenizing denture cleaners. However, synthetic denture cleaner have several limitation due to its biocompatibility on human body especially oral tissue. Chitosan is a derivate of chitin, a natural compound of sea creature exosceleton. Chitosan have antifungi and antibacterial nature. The objective of this study is to evaluate the antifungi efectifity of chitosan 2% on acrylic heatcured and compare it with oxygenizing denture cleaner. Aquades is used as a control. The sample consists of 15 acrylic heatcured bars (10x10x2 mm) prepared by contamination on Candida albicans suspensions at temperature 37°C for 24 hours and immersed on 3 different treatments wich are chitosan 2%, oxygenizing denture cleanser, and aquades as control and then, the solutions are being vibrate and put into sabouraud dextrose agar medium. The colony of Candida albicans counted manually. Result shows, highest mean of Candida albicans colony on aquades group (365,66 CFU/plate), followed by oxygenizing denture cleanser treatment (178,33 CFU/plate). The smallest number colony of Candida albicans are found at chitosan 2% treatment (45,33 CFU/plate). Statistical analysis using ANOVA one way shows F=9.826 and p=0, this means, there is minimal one pair of groups with very significant differential (p<0,01) which are chitosan 2% and oxygenizing denture cleanser compared to aquades as control. The difference between those two treatments also significant. Conclusions of this study is that chitosan 2% solution have a sifnificant effect on prohibiting the growth of Candida albicans colony on acrylic heatcured denture base materials. The effectivity of this solution is significantly higher than oxygenizing denture cleanser treatment.

scholarly journals Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability

2012 ◽  
Vol 23 (5) ◽  
pp. 547-554 ◽  
Author(s):  
Delise Pellizzaro ◽  
Gregory Polyzois ◽  
Ana Lucia Machado ◽  
Eunice Teresinha Giampaolo ◽  
Paula Volpato Sanitá ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Reduction Method ◽  
Acrylic Resin ◽  
Chlorhexidine Gluconate ◽  
Common Disease ◽  
Combined Method ◽  
Denture Stomatitis ◽  
Positive Controls

The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (10(7) cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions.

scholarly journals Kinetics of antifungal activity of home-generated ozonated Water on Candida albicans

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Amirtaher Mirmortazavi ◽  
Hamidreza Rajati Haghi ◽  
Abdolmajid Fata ◽  
Hossein Zarrinfar ◽  
Hossein Bagheri ◽  
...  
Keyword(s):  
Oral Candidiasis ◽  
Test Group ◽  
Acrylic Resin ◽  
Positive Control ◽  
Negative Control ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Acrylic Resins ◽  
Colony Forming Units ◽  
Ozonated Water

Background and Purpose: Candida-associated denture stomatitis is one of the most common forms of oral candidiasis among denture wearers. Regarding this, the aim of the present study was to evaluate the antifungal effects of home-generated ozonated water on the adhesion of the C. albicans attached to the surface of the denture base acrylic resins. Materials and Methods: For the purpose of the study, different concentrations of C. albicans were added to the tubes containing acrylic resin blocks, and then incubated for 2 h at 35°C. The samples were assigned into three groups, each of which contained 42 samples, including normal saline (NS) solution as the negative control, nystatin (N) solution as the positive control, and ozonated water as the test group. The samples were washed and placed in an ultrasonic bath. Subsequently, the saline solution was cultured on Sabouraud dextrose agar. The concentrations of Candida were evaluated during the contact times. Results: The test group (i.e., ozonated water) with 114 colony-forming units (CFU) showed a significant reduction of Candida colonies, compared to the NS group with 2,172 CFU. The 120- and 1-minute incubation with ozonated water showed the highest and lowest effects on the viability of Candida adhered to the acrylic resin, respectively. Conclusion: Based on the findings, home-generated ozonated water can be applied to remove the Candida attached to the surface of the denture plates. Keywords: Antifungal, Candida, Denture, Ozonated water, Stomatitis

scholarly journals Antibiofilm and Protein-Repellent Polymethylmethacrylate Denture Base Acrylic Resin for Treatment of Denture Stomatitis

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1067
Author(s):  
Salwa O. Bajunaid ◽  
Bashayer H. Baras ◽  
Abdulrahman A. Balhaddad ◽  
Michael D. Weir ◽  
Hockin H. K. Xu
Keyword(s):  
Surface Roughness ◽  
Acrylic Resin ◽  
Control Group ◽  
Solution Ph ◽  
Biofilm Growth ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Colony Forming Unit ◽  
Microbial Infections ◽  
Unit Reduction

Candida albicans (C. albicans) biofilm is a common etiological factor in denture stomatitis. The purpose of this study was to investigate the effects of incorporating 2-methacryloyloxyethyl phosphorylcholine (MPC) as a protein repellent into a new high-impact denture acrylic (HIPA) resin on the surface roughness, solution pH, and C. albicans biofilm adhesion to the denture base. The new acrylic denture resin base was formulated by mixing MPC into HIPA resin at mass fractions of 1.5%, 3%, and 4.5%. Surface roughness was measured using a Mitutoyo surface roughness tester. C. albicans biofilm growth and viability were assessed via colony forming unit counts. The pH of the biofilm growth medium was measured using a digital pH meter. Adding MPC to the HIPA resin at percentages of 1.5% and 3% increased the roughness values significantly (p < 0.05), while adding 4.5% MPC resulted in no difference in roughness values to that of the control group (p > 0.05). All experimental groups demonstrated neutral pH values (pH ≅ 7) and were not significantly different from each other (p > 0.05). Incorporating 2-methacryloyloxyethyl phosphorylcholine at 4.5% resulted in a significant (≅1 log) colony-forming unit reduction compared with the control group with 0% MPC (p < 0.05). A fungal-retarding denture acrylic resin was developed through the incorporation of MPC for its protein-repelling properties. This newly developed denture acrylic material has the potential to prevent oral microbial infections, such as denture stomatitis.

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