Uji Sitotoksisitas Seng Oksida Ekstrak Allium Sativum Linn. terhadap Sel Fibroblas Gingiva Manusia

The use of zinc oxide eugenol in open pulps is controversial because of the cytotoxic effects of eugenol. Previous research has proven that zinc oxide Allium sativum Linn. extract can be an alternative for pulp capping material because it has antibacterial effect. The materials that applied to the oral cavity must be non-cytotoxic and biocompatible, therefore cytotoxicity testing is required of zinc oxide Allium sativum Linn. extract on human gingival fibroblast cell. To determine the cytotoxicity of zinc oxide Allium sativum Linn. extract on human gingival fibroblast cell. Cytotoxicity test of a mixture of zinc oxide with Allium sativum Linn. extract using ratio 1:1 (group A) and 2:1 (group B), zinc oxide eugenol 1:2 (control group C). Each material is exposed to the human gingival fibroblast cells. The cytotoxicity test was tested using an MTT essay test. The density of optic formazan indicated the number of living cell. Cytotoxicity parameters using IC50. Data were analyzed using one-way Anova test and Tukey HSD test. The number of living cells in group A= 40,400 %, group B= 50,613 % and group C= 51,247 %. There was no significant difference between zinc oxide Allium sativum Linn at a ratio of 2:1 (group B) with zinc oxide eugenol 2:1 (group C). Zinc oxide Allium sativum Linn. extract with a ratio 2:1 were not cytotoxic toward human gingival fibroblast cell.

Pengaruh Lama Perendaman E-Glass Fiber Reinforced Composite dalam Obat Kumur Terhadap Kekuatan Fleksural

Fiber reinforced composite (FRC) is a material consisting of a composite resin (matrix), fiber (reinforced) and silanes (to improve the adhesion strength). FRC can be used as a crown framework. More people used mouthwash for oral hygient. The objective of this study was to know the effect of the duration of E-glass FRC immersion into the mouthwash against the flexural strength. The research materials were E-glass (Fiber Splint Polydentia Multi-Layer, Switzerland), composite resin (Master Flow Biodynamic, Brazil), silane (Monobond Ivoclar Vivadent) and mouthwash (Listerine® Multi Protect). Twelve the beam-shaped specimens (25x2x2) mm The specimens were divided into 3 groups: K1 control group (was not immersed) K2 and K3 treatment groups (was immersed for 24 and 48 hours). The flexural strength value was measured using a universal testing machine (Pearson, Texas). The data were analyzed using one-way ANOVA followed-up by LSD. The results showed decrease of the average flexural strengths of mouthwash K1, K2 and K3. The one-way ANOVA test results showed that there was a significant P>0.05 in the duration of E-glass FRC immersion into the mouthwash against the flexural strength. The LSD test results showed significant differences between all treatment groups. The conclusion of this study is that the duration of E-glass FRC immersion into mouthwash will lower the strength of flexural E-glass FRC.

Perbandingan Aktivitas Antibakteri Ekstrak Curcuma zedoaria dan Bahan Irigasi Natrium Hipoklorit 2.5% terhadap Enterococcus faecalis

Sodium hypochlorite (NaOCl) is a powerful disinfectant used in root canal therapy, yet it has some disadvantages. There are growing interests of using extracts of natural materials containing antibacterial secondary metabolite compounds as an alternative of irrigation solution. Aim, to compare the antibacterial activity of white turmeric extracts and 2,5% sodium hypochlorite irrigant against Enterococcus faecalis. Methods, this was a quasi experimental study. White turmeric extracts at the concentrations of 75%, 50%, 25% and 10% were used as experimental groups, while 2,5% NaOCl and distilled water were the control groups. Suspensions of Enterococcus faecalis were prepared to a 0.5 Mc Farland standard and applied onto petri plates containing Muller Hinton Agar. Six wells were created in each plate, and filled with 20 mL of the test solution. Plates were incubated at 37 ° C for 24 hours. The antibacterial activity was evaluated by measuring the clear zone around the well. Data were analyzed by Kruskall Wallis and continued by Mann U Whitney. Results, the largest growth inhibition zones were produced when the test bacteria were in contact with 2,5% NaOCl (15,0233 mm). White turmeric extract 75% concentration had weak antibacterial effect, while white turmeric extract 50%, 25%, 10% concentration, and distilled water did not has antibacterial properties against Enterococcus faecalis. Conclusion, white turmeric extract 75% concentration can inhibit the growth of Enterococcus faecalis with weak antibacterial activity.

Pengaruh Nanofilled Resin Coating terhadap Kekasaran Permukaan Semen Ionomer Kaca

One of the restorative dental materials that is continuously developed is Glass Ionomer Cement (GIC). The aim of this research is to know whether there is influence of nanofilled resin coating application on GIC to reduce surface roughness. In this study GIC type II (EQUIA Forte, GC, Japan) was used to be mechanically manipulated and inserted into a mold which produced a sample of 6.0±0.3 mm in diameter and 3.0±0.2 mm in height. GIC samples were divided into 2 groups namely 10 GIC samples were not coated with nanofilled resin coating as control group and 10 GIC samples were coated with nanofilled resin coating as treatment group. GIC samples were tested immediately and then immersed in sterile distilled water in a 37°C incubator. After 24 hours, GIC samples were surface roughness tested using a Surface Roughness Tester. Then all samples were brushed using a soft bristle mechanical tooth brush for 1 hour. After brushing, the sample is tested for final surface roughness. The results showed an increase in surface roughness in non-coated group/control (0.005 ± 0,328mm) and coated group (0.015±0.240 mm). Statistical data analysis using unpaired t test showed that there was no significant difference (p>0.05) in the increasing of surface roughness between control group and treatment group. It can be concluded that application of nanofilled resin coating to GIC cannot reduce the roughness of GIC surface.

Pengaruh Desinfeksi Energi Microwave Terhadap Kekuatan Transversal Resin Akrilik Polimerisasi Panas

Disinfectant by microwave energy is highly recommended as a solution to solve the problem of chemical disinfection on denture bases. One of the denture base materials is heat cured acrylic resin. Aim, to evaluate the effect of disinfectant by microwave energy with different time on the transversal strength of heat cured acrylic resin. 30 plates (n = 6) of heat cured acrylic resin with size 65x10x2,5 mm were divided into 5 groups (control; and microwave energy disinfected for 2, 4 , 6, and 8 minutes with 650 Watts of power). Transversal strength test was carried out using Torsee's Electronic System Universal Testing Machine with a test speed of 0.1 mm / second, and a load cell of 50 kgf. Data analyzed using one-way ANOVA and LSD pos hoc. Results, there were significant differences in the transverse strength among all groups (p <0.05). Mean values of all groups were 103.59 ± 0.66 MPa; 112.02 ± 2.10 MPa; 117.48 ± 1.73 MPa; 125.46 ± 2.25 MPa; and 135.89 ± 1.56 MPa respectively. Conclusion, the longer it is disinfected by microwave energy, the higher the transversal strength of heat cured acrylic resin.

Sitotoksisitas Kombinasi Seng-oksida dan Ekstrak Curcuma longa Terhadap Fibroblas Gingiva Manusia

The use of zinc oxide - eugenol as periodontal dressing is controversial due to the toxic nature of eugenol and Curcuma longa Linn as a safe natural herbal, is expected to replace eugenol function. Previous research has proven that the combination of zinc oxide with Curcuma liquid extract has an antibacterial properties and the use of those as wound dressing through in vivo studies proved to accelerate wound healing. The ingredients applied to the oral cavity must benon-toxic and biocompatible, therefore cytotoxicity testing 1 is required. To determine the cytotoxicity of zinc oxide and Curcuma longa extract combination on human gingival fibroblast cells. Cytotoxicity test of combination of zinc oxide powder with Curcuma liquid extract was done at ratio of 1: 1 (group A) and 2: 1 (group B), Curcuma liquid extract (group C) and zinc oxide powder (group D), which performed on human gingival fibroblast culture-cells (3-5x103 densities) in 96 wells microplate. MTT essay was used to analyzed with IC50 parameters. Statistical analysis using oneway Anova and LSD. The number of living cells in group A = 64.819%, group B = 59.452%, group C = 52.859% and group D = 60.502%. There was no significant difference between the groups of A, B, D but those groups were significantly different to group C. Combination of zinc oxide with Curcuma extract ratio of 1: 1 and 2: 1, zinc oxide powder and Curcuma liquid extract are not toxic to human gingival fibroblast at IC50.

Perubahan Warna Permukaan Resin Komposit Nanohybird Pasca Perendaman Dalam Cuko Pempek

The development of science, technology and patient demands regarding restorative materials that are colored with teeth is currently higher. Dental-colored restoration materials are widely used, one of which is nanohybrid composite resin. The nanohybrid composite resin has a small and fine particle size of 0.04 um. Nanohybrid composite resins have many advantages, but they also have the disadvantage of liquid absorption properties which can cause discoloration. One of the liquids that cause discoloration is cuko pempek. Objective: To find out the surface color changes of nanohybrid composite resin after immersion in cuko pempek. Methods, an experimental laboratory study with a pre and post test only group design study design. Sample 16 pieces of nanohybrid composite resin measuring 10 x 2 mm. The sample was immersed using pempek cuko as much as 5 ml with a temperature of 37oC for 7 days. Color change is measured with a spechtrophotometer (UV-2401 PC). Results, there was a change in the surface color of nanohybrid composite resin soaked with pempek cuko which marked an average increase in dE*ab before immersion (18.5925) and after immersion (23.3169) with a difference of 4.7844. The paired t test results also showed a significant color change that is p = 0,000 (p <0.05). Conclusion, there is a change in the surface color of the post-immersion nanohybrid composite resin in pempek cuko.

Evaluasi Nilai Kekerasan Resin Komposit Bulkfill dengan Variasi Waktu Penyinaran Sinar LED

Bulkfill composite resin can be applied in “bulk” up to 4mm and it can be cures with LED curing lights in 10 seconds. Time exposure affects the hardness of the composite resin material. This experiment aims to determine differences in exposure time with LED on the hardness of bulkfill composite resin. This experiment is a true experiment. The samples are bulkfill composite resin with diameter of 6 mm and thickness of 4 mm. There were 3 groups of samples (n=5) and cured with LED with intensity 1000mW/cm2. Group 1 was exposed for 10 seconds, group 2 for 20 seconds, and group 3 for 40 seconds. The samples were soaked in distilled water 37oC for 24 hours in waterbath. Microvickers hardness with the load of 100 grams in 15 seconds applied to the samples on the lower and the upper surface. The result found that the ratio of hardness both 10 seconds and 20 seconds, and 20 seconds and 40 seconds are no significant. The ratio hardness of 10 seconds and 40 seconds had significant difference. Based on the research irradiation time effect resin bulkfill composite hardness. It means that the more monomer polymerized the more higher hardness could be achived. The longer exposure time can make increases hardness level.

The Evaluation of Flow Property of Alginate Impression Material Mixed with Gargle Solutions

The effects of antiseptic and gargle solutions mixed with the irreversible hydrocolloid alginate impression material to anti-bacterial activity and several properties had been elucidated. The flow property of the such impression has been not elucidated yet. The aim of the study was to evaluate the flow property of alginate impression mixed with gargle solution. Aroma Fine Plus alginate impression material was used and mixed with aquadest as control and Minosep, Betadine and Total Care gargle solutions. Ratio between alginate and each solutions were performed by following the producer’s guidance as 8.4gr alginate and 20ml solution. The every alginate mixtures were made in triplicate (n=3). Measurement of flow capability was performed by using 0.5ml of alginate mix laid on glass plate covered with cellophane strip and loaded with 1kg metal for 1 minute. The major and minor diameters were measured by using digital caliper. Data were analyzed by using one way ANOVA (α= 0.05). Aroma Fine mixed with aquadest showed the flow capability was about 26.18mm. Mixed with Minosep showed about 27.85mm, with Betadine 29.19mm and Total Care 27.11mm respectively. Statistically show a significant different flow capability between aquadest and Betadine mixed impression (p=0.01). Minosep and Total Care gargle solutions showed insignificant different with aquadest mixed impression (p>0.05). It can be concluded that the utilizing of Minosep and Total care gargle solution as solutions mixed with alginate impression material have no effect to flow property of the impression, except by using Betadine gargle solution.

Pengaruh Lama Perendaman dalam Obat Kumur Terhadap Kekerasan Polyethylene Fiber-Reinforced Composites

Fiber-reinforced composites (FRC) is a resin base material that strengthen with fiber. In dentistry, these materials interacts with various fluid, such as mouthwash. Mouthwash that contains alcohol affects the physical and mechanical properties of the material. The aim of this research is to determine the effect of immersion duration in mouthwash towards the hardness of polyethylene fiber reinforced composites. The materials used in this research are polyethylene (Fiber UHMWPE, Vactrise), composite resin (MasterFlow, Brazil), and mouthwash (Original Listerine® Antiseptic). Strip-shaped specimens (5x2x2)mm is light cured for 20 seconds. The specimens is divided into K1 (control group), K2 (immersed for 24 hours) and K3 (immersed for 48 hours). Hardness values is measured using Vickers Hardness Test (Buehler, Germany). The data obtained is analyzed using one way anova and followed by LSD test. The results showed the mean of hardness of groups immersed with mouthwash (0, 24, and 48 hours) are 19±0.82 VHN, 14.33±0.25 VHN, and 8.67±0.84 VHN respectively. One way anova test showed there is a significant effect (p<0,05) of immersion duration in mouthwash towards the hardness of polyethylene FRC. LSD test showed significant differences between all groups. The conclusion of this research is prolonged immersion in mouthwashes decreases the hardness of polyethylene FRC.