Adherence in vitro of Candida albicans to plasma treated acrylic resin. Effect of plasma parameters, surface roughness and salivary pellicle

Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.

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In Vitro Antifungal Evaluation of Seven Different Disinfectants on Acrylic Resins

BioMed Research International ◽

10.1155/2014/519098 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

A. Z. Yildirim-Bicer ◽

I. Peker ◽

G. Akca ◽

I. Celik

Keyword(s):

Surface Roughness ◽

Candida Albicans ◽

Uv Light ◽

Acrylic Resin ◽

Cost Effective ◽

Alternative Methods ◽

Acrylic Resins ◽

Significant Difference ◽

White Vinegar

Objective. The aim of this study was to evaluate alternative methods for the disinfection of denture-based materials.Material and Methods. Two different denture-based materials were included in the study. Before microbial test, the surface roughness of the acrylic resins was evaluated. Then, the specimens were divided into 8 experimental groups(n=10), according to microorganism considered and disinfection methods used. The specimens were contaminated in vitro by standardized suspensions ofCandida albicansATCC#90028 andCandida albicansoral isolate. The following test agents were tested: sodium hypochlorite (NaOCl 1%), microwave (MW) energy, ultraviolet (UV) light, mouthwash containing propolis (MCP), Corega Tabs, 50% and 100% white vinegar. After the disinfection procedure, the number of remaining microbial cells was evaluated in CFU/mL. Kruskal-Wallis, ANOVA, and Dunn’s test were used for multiple comparisons. Mann WhitneyUtest was used to compare the surface roughness.Results. Statistically significant difference(P<0.05)was found between autopolymerised and heat-cured acrylic resins. The autopolymerised acrylic resin surfaces were rougher than surfaces of heat-cured acrylic resin. The most effective disinfection method was 100% white vinegar for tested microorganisms and both acrylic resins.Conclusion. This study showed that white vinegar 100% was the most effective method for tested microorganisms. This agent is cost-effective and easy to access and thus may be appropriate for household use.

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In Vitro Adherence of Candida albicans to Acrylic Resin with Different Surface Status

Interface Oral Health Science 2011 ◽

10.1007/978-4-431-54070-0_36 ◽

2012 ◽

pp. 145-146

Author(s):

Taro Nomura ◽

Tetsuya Suzuki ◽

Junichi Furuya ◽

Yu Shimoyama ◽

Minoru Sasaki ◽

...

Keyword(s):

Candida Albicans ◽

Acrylic Resin

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In vitro evaluation of microbial adhesion on the different surface roughness of acrylic resin specific for ocular prosthesis

European Journal of Dentistry ◽

10.4103/ejd.ejd_50_18 ◽

2018 ◽

Vol 12 (02) ◽

pp. 176-183 ◽

Cited By ~ 5

Author(s):

Agda Marobo Andreotti ◽

Cecília Alves De Sousa ◽

Marcelo Coelho Goiato ◽

Emily Vivianne Freitas da Silva ◽

Cristiane Duque ◽

...

Keyword(s):

Surface Roughness ◽

Staphylococcus Epidermidis ◽

Microbial Growth ◽

Acrylic Resin ◽

Microbial Adhesion ◽

Force Microscopy ◽

Atomic Force ◽

Colony Forming Units ◽

Ocular Prostheses

ABSTRACT Objective: The purpose of this study was to evaluate the influence of surface roughness in biofilm formation of four microorganisms (Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis, and Candida albicans) on acrylic resin surface of ocular prostheses. Materials and Methods: Acrylic resin samples were divided into six groups according to polishing: Group 1200S (1200 grit + silica solution); Group 1200; Group 800; Group 400; Group 120 and Group unpolished. Surface roughness was measured using a profilometer and surface images obtained with atomic force microscopy. Microbial growth was evaluated after 4, 24, and 48 hours of incubation by counting colony-forming units. Statistical Analysis Used: For roughness, it was performed 1-way ANOVA and parametric Tukey test α5% (P ≤ 0.05). For CFU data found, it was applied Kruskal-Wallis and Mann-Whitney tests. Results: Group 120 and 400 presented the highest roughness values. For S. epidermidis and S. aureus, Group 1200S presented the lowest values of microbial growth. For E. faecalis at 4 hour, microbial growth was not observed. C. albicans did not adhere to the acrylic resin. Except for Group 1200S, different surface roughnesses did not statistically interfere with microbial adhesion and growth on acrylic surfaces of ocular prostheses. Conclusions: The roughness did not interfere with the microbial adhesion of the microorganisms evaluated. The use of silica decreases significantly microbial growth.

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Effect of human whole saliva on the in vitro adhesion of Candida albicans to a denture base acrylic resin: a focus on collection and preparation of saliva samples

Journal of Investigative and Clinical Dentistry ◽

10.1111/jicd.12006 ◽

2012 ◽

Vol 4 (4) ◽

pp. 225-228 ◽

Cited By ~ 4

Author(s):

Camila A. Zamperini ◽

Patricia C. S. Schiavinato ◽

Ana C. Pavarina ◽

Eunice T. Giampaolo ◽

Carlos E. Vergani ◽

...

Keyword(s):

Candida Albicans ◽

Acrylic Resin ◽

Denture Base ◽

Whole Saliva ◽

In Vitro Adhesion

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Antifungal Effect of Silver Nitrate on Prosthodontic Dentures

Balkan Journal of Dental Medicine ◽

10.2478/bjdm-2020-0016 ◽

2020 ◽

Vol 24 (2) ◽

pp. 96-101

Author(s):

Mahmut Sertac Ozdogan ◽

Mustafa Gumusok ◽

Mihriban Yucel ◽

Bedia Dinc ◽

Muzaffer Mizrak ◽

...

Keyword(s):

Candida Albicans ◽

Silver Nitrate ◽

Sodium Hypochlorite ◽

Acrylic Resin ◽

Chlorhexidine Digluconate ◽

Promising Alternative ◽

Antifungal Effect ◽

Colony Forming Unit ◽

Colony Counting

SummaryBackground/Aim: Although there are studies about the antimicrobial activity of silver, there is no study evaluating it as a denture disinfectant. The purpose of this study was to explore the effectiveness of 6 disinfectant solutions (50% vinegar, 100% vinegar, 1% silver nitrate, 2% silver nitrate, %1 sodium hypochlorite, 0,12% chlorhexidine digluconate) in the disinfection of acrylic resin specimens contaminated in vitro by Candida albicans, as measured by residual colony-forming unit (CFU).Material and Methods: 66 pieces of 10mmx2mm acrylic resin disc samples were prepared and incubated in 1x106 cell/ml suspension of C. albicans ATCC 18804 for 24 h (one of them as a control, n=11/group). The specimens were then transferred into tubes containing 10 ml of the tested disinfectants and kept for 10 minutes in the disinfectant. After washing with saline, the specimens were vortexed to remove the microorganisms adhered to the surfaces. Colony counting of the collected microorganisms was performed on Sabouroud dextrose medium using 10−1, 10−2 and 10−3 dilutions. The results were analysed using Kruskal-Wallis and Mann-Whitney U tests (p<0,05).Results: The results showed that 1% sodium hypochlorite, %1 silver nitrate and 2% silver nitrate were the most effective against Candida Albicans (p<0,05), followed by 100% vinegar, 0,12% chlorhexidine digluconate and 50% vinegar (p<0,05).Conclusions: Within the boundaries of this study, we conclude that 1% silver nitrate is a promising alternative disinfectant to 1% sodium hypochlorite and performs better compared to 0,12 % chlorhexidine gluconate, 50% and 100% vinegar.

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Antimicrobial and mechanical acrylic resin properties with silver particles obtained from Fusarium oxysporum

Brazilian Dental Science ◽

10.14295/bds.2018.v21i1.1534 ◽

2018 ◽

Vol 21 (1) ◽

pp. 96 ◽

Cited By ~ 1

Author(s):

Tabata Prado Sato ◽

Celio Ilidio Conjo ◽

Rodnei Dennis Rossoni ◽

Juliana Campos Junqueira ◽

Renata Marques de Melo ◽

...

Keyword(s):

Surface Roughness ◽

Fusarium Oxysporum ◽

Biofilm Formation ◽

Antimicrobial Agents ◽

Acrylic Resin ◽

Control Group ◽

Significant Difference ◽

Color Alteration ◽

Cie Lab

Objective: This study evaluated the effects of the incorporation of silver nanoparticles (AgNPs) obtained from Fusarium oxysporum on heat-activated acrylic resin (HAAR) and their influence on resin’s surface roughness, hardness, color alteration and antimicrobial capacity against Candida albicans. Material and Methods: For this, 50 discs of HAAR (2x5 mm) were produced and divided into three groups, Control: HAAR; Ag1: HAAR plus 0.539 mg of AgNPs; and Ag2: HAAR plus 1.1 mg of AgNPs. Knopp hardness (HK), surface roughness (Ra and Rz) and color alteration according to the CIE Lab were measured. Specimens were then evaluated in vitro with regard to C. albicans biofilm formation through formed colony count (CFU/mL). Scanning Electron Microscopy (SEM) and Atomic force microscopy (AFM) analyses were performed. Results: The addition of AgNPs of both concentrations changed Ra, Rz and HK significantly. There was statistically significant difference for L (p=0.00); a*(p=0.00) and b*(p=0.00) parameters. There were no differences between Ag1 and Ag2 biofilm formation, but the comparison of both with the control group presented a significant reduction (p=0.0091) on biofilm formation. SEM and AFM images showed no signs of NPs clustering. Conclusion: It can be concluded tha AgNPs incorporation in HAAR was effective in reducing C. albicans activity, with a slight change in color and hardness of the material, being effective therefore, in regions such as the dental prostheses palate, which have lesser aesthetic appeal.KeywordsAcrylic resins; Silver; Antimicrobial agents.

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Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability

Brazilian Dental Journal ◽

10.1590/s0103-64402012000500013 ◽

2012 ◽

Vol 23 (5) ◽

pp. 547-554 ◽

Cited By ~ 16

Author(s):

Delise Pellizzaro ◽

Gregory Polyzois ◽

Ana Lucia Machado ◽

Eunice Teresinha Giampaolo ◽

Paula Volpato Sanitá ◽

...

Keyword(s):

Candida Albicans ◽

Biofilm Formation ◽

Reduction Method ◽

Acrylic Resin ◽

Chlorhexidine Gluconate ◽

Common Disease ◽

Combined Method ◽

Denture Stomatitis ◽

Positive Controls

The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (10(7) cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions.

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Effect of Different Dentifrices on the Surface Roughness of Acrylic Resins: An in vitro Study

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-2106 ◽

2017 ◽

Vol 18 (8) ◽

pp. 679-682

Author(s):

Nitin Gautam ◽

Aditi Sharma ◽

Unjum Bashir ◽

Sisir Rapolu ◽

Nallamilli Mamatha ◽

...

Keyword(s):

Surface Roughness ◽

In Vitro Study ◽

Positive Association ◽

Acrylic Resin ◽

Control Group ◽

Distilled Water ◽

Acrylic Resins ◽

Type Size ◽

Utmost Care

ABSTRACT Aim This study was undertaken to study the effect of three most commonly used dentifrices for denture cleaning and to find out any positive association between number of strokes by toothbrush and surface roughness of acrylic resin. Materials and methods About 40 acrylic rectangular blocks with high finish were polished until surface roughness was calculated as 0 µm. The materials tested were distilled water as a control group, Patanjali Dant Kanti, Colgate Total Cream, and Cleansodent as dentifrices. Stroking was done with dentifrices, and surface roughness readings were recorded at 1,000, 9,000, and 18,000 strokes. Results Patanjali Dant Kanti was found to have maximum abrasive potential followed by Colgate Total Cream, followed by Cleansodent and least by distilled water. Conclusion Surface roughness was found to be directly proportional to the type, size, and fineness of the abrasive component used; the professional denture cleanser (Cleansodent) has less abrasive potential than the regular toothpastes. Among those tested, the highest abrasive potential was found in Patanjali Dant Kanti and lowest by Cleansodent (denture cleanser). Clinical significance The patient as well as dentist should choose the dentifrice with utmost care noting the size and type of abrasives used. Washing the dentures normally with water without toothbrush daily and cleaning of dentures once in 10 to 15 days with a soft toothbrush and professional denture cleanser should be the line of maintenance of dentures. How to cite this article Gautam N, Sharma A, Bashir U, Rapolu S, Mamatha N, Sravani S. Effect of Different Dentifrices on the Surface Roughness of Acrylic Resins: An in vitro Study. J Contemp Dent Pract 2017;18(8):679-682.

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