scholarly journals Novel Bioactive Peptides from Meretrix meretrix Protect Caenorhabditis elegans against Free Radical-Induced Oxidative Stress through the Stress Response Factor DAF-16/FOXO

Marine Drugs ◽  
2018 ◽  
Vol 16 (11) ◽  
pp. 444 ◽  
Author(s):  
Weizhang Jia ◽  
Qiong Peng ◽  
Linnan Su ◽  
Xuesong Yu ◽  
Chung Ma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Stress Response ◽  
Antioxidant Enzyme ◽  
Random Coil ◽  
Enzyme Analysis ◽  
Hard Clam ◽  
Response Factor ◽  
Meretrix Meretrix ◽  
Antioxidant Peptides

The hard clam Meretrix meretrix, which has been traditionally used as medicine and seafood, was used in this study to isolate antioxidant peptides. First, a peptide-rich extract was tested for its protective effect against paraquat-induced oxidative stress using the nematode model Caenorhabditis elegans. Then, three novel antioxidant peptides; MmP4 (LSDRLEETGGASS), MmP11 (KEGCREPETEKGHR) and MmP19 (IVTNWDDMEK), were identified and were found to increase the resistance of nematodes against paraquat. Circular dichroism spectroscopy revealed that MmP4 was predominantly in beta-sheet conformation, while MmP11 and MmP19 were primarily in random coil conformation. Using transgenic nematode models, the peptides were shown to promote nuclear translocation of the DAF-16/FOXO transcription factor, a pivotal regulator of stress response and lifespan, and induce the expression of superoxide dismutase 3 (SOD-3), an antioxidant enzyme. Analysis of DAF-16 target genes by real-time PCR reveals that sod-3 was up-regulated by MmP4, MmP11 and MmP19 while ctl-1 and ctl-2 were also up-regulated by MmP4. Further examination of daf-16 using RNA interference suggests that the peptide-increased resistance of C. elegans to oxidative stress was DAF-16 dependent. Taken together, these data demonstrate the antioxidant activity of M. meretrix peptides, which are associated with activation of the stress response factor DAF-16 and regulation of the antioxidant enzyme genes.

scholarly journals Antioxidant Peptides from Sepia esculenta Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in Caenorhabditis elegans

Marine Drugs ◽  
2020 ◽  
Vol 18 (10) ◽  
pp. 490
Author(s):  
Xuesong Yu ◽  
Qina Su ◽  
Tianqi Shen ◽  
Qiong Chen ◽  
Ying Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liquid Chromatography ◽  
Caenorhabditis Elegans ◽  
High Performance ◽  
Gel Filtration ◽  
Antioxidant Peptides ◽  
Sod Activity ◽  
Fat Accumulation ◽  
C Elegans ◽  
Sepia Esculenta

The hydrolysate of golden cuttlefish (Sepia esculenta) was prepared by using papain, and then, it was further separated by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). The peptide components of the active fraction were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and then two novel peptides, SeP2 (DVEDLEAGLAK, 1159.27 Da) and SeP5 (EITSLAPSTM, 1049.22 Da), were obtained and displayed significant alleviation effects on oxidative stress in Caenorhabditis elegans. Studies indicated that S. esculenta antioxidant peptides (SePs) increase superoxide dismutase (SOD) activity but reduce reactive oxygen species (ROS) and malondialdehyde (MDA) levelsin oxidation-damaged nematodes. Using transgenic CF1553 nematodes, the sod-3p::GFP expression in the worms treated with SePs was significantly higher than that of the control nematodes. Real-time PCR also demonstrated that the expression of stress-related genes such as sod-3 is up-regulated by SePs. Furthermore, studies showed that SePs could obviously decrease fat accumulation as well as reduce the elevated ROS and MDA levels in high-fat nematodes. Taken together, these results indicated that SePs are capable of the activation of antioxidant defense and the inhibition of free radicals and lipid peroxidation, play important roles in attenuating oxidative stress and fat accumulation in C. elegans, and might have the potential to be used in nutraceutical and functional foods.

scholarly journals Two novel antioxidant peptides derived from Arca subcrenata against oxidative stress and extend lifespan in Caenorhabditis elegans

2021 ◽  
Vol 81 ◽  
pp. 104462
Author(s):  
Hui Shi ◽  
Xuqiao Hu ◽  
Hang Zheng ◽  
Chunlei Li ◽  
Lianli Sun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Antioxidant Peptides

scholarly journals The Caenorhabditis elegans Oxidative Stress Response Requires the NHR-49 Transcription Factor

2018 ◽  
pp. g3.200727.2018 ◽  
Author(s):  
Queenie Hu ◽  
Dayana R. D'Amora ◽  
Lesley T. MacNeil ◽  
Albertha J. M. Walhout ◽  
Terrance J. Kubiseski
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Caenorhabditis Elegans ◽  
Stress Response ◽  
Oxidative Stress Response

Proteasomal dysfunction activates the transcription factor SKN-1 and produces a selective oxidative-stress response in Caenorhabditis elegans

2007 ◽  
Vol 409 (1) ◽  
pp. 205-213 ◽  
Author(s):  
Nate W. Kahn ◽  
Shane L. Rea ◽  
Sarah Moyle ◽  
Alison Kell ◽  
Thomas E. Johnson
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Stress Response ◽  
Nuclear Localization ◽  
Glutathione Transferase ◽  
Fluorescent Protein ◽  
Oxidative Stress Response ◽  
Specific Gene ◽  
Related Factor ◽  
Green Fluorescent

SKN-1 in the nematode worm Caenorhabditis elegans is functionally orthologous to mammalian NRF2 [NF-E2 (nuclear factor-E2)-related factor 2], a protein regulating response to oxidative stress. We have examined both the expression and activity of SKN-1 in response to a variety of oxidative stressors and to down-regulation of specific gene targets by RNAi (RNA interference). We used an SKN-1–GFP (green fluorescent protein) translational fusion to record changes in both skn-1 expression and SKN-1 nuclear localization, and a gst-4–GFP transcriptional fusion to measure SKN-1 transcriptional activity. GST-4 (glutathione transferase-4) is involved in the Phase II oxidative stress response and its expression is lost in an skn-1(zu67) mutant. In the present study, we show that the regulation of skn-1 is tied to the protein-degradation machinery of the cell. RNAi-targeted removal of most proteasome subunits in C. elegans caused nuclear localization of SKN-1 and, in some cases, induced transcription of gst-4. Most intriguingly, RNAi knockdown of proteasome core subunits caused nuclear localization of SKN-1 and induced gst-4, whereas RNAi knockdown of proteasome regulatory subunits resulted in nuclear localization of SKN-1 but did not induce gst-4. RNAi knockdown of ubiquitin-specific hydrolases and chaperonin components also caused nuclear localization of SKN-1 and, in some cases, also induced gst-4 transcription. skn-1 activation by proteasome dysfunction could be occurring by one or several mechanisms: (i) the reduced processivity of dysfunctional proteasomes may allow oxidatively damaged by-products to build up, which, in turn, activate the skn-1 stress response; (ii) dysfunctional proteasomes may activate the skn-1 stress response by blocking the constitutive turnover of SKN-1; and (iii) dysfunctional proteasomes may activate an unidentified signalling pathway that feeds back to control the skn-1 stress response.

Oxidative stress response, epigenetic and behavioral alterations in Caenorhabditis elegans exposed to organophosphorus pesticide quinalphos

2019 ◽  
Vol 17 ◽  
pp. 702-709 ◽  
Author(s):  
Duraipandian Govindarajan ◽  
Chitral Chatterjee ◽  
Ganeshan Shakambari ◽  
Perumal Varalakshmi ◽  
Kannan Jayakumar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Stress Response ◽  
Organophosphorus Pesticide ◽  
Oxidative Stress Response ◽  
Behavioral Alterations

scholarly journals Oxidative stress resistance and fitness-compensatory response in vancomycin-intermediate Staphylococcus aureus (VISA)

2018 ◽  
Author(s):  
Xin-Ee Tan ◽  
Hui-min Neoh ◽  
Longzhu Cui ◽  
Keiichi Hiramatsu ◽  
Rahman Jamal
Keyword(s):  
Oxidative Stress ◽  
Staphylococcus Aureus ◽  
Caenorhabditis Elegans ◽  
Stress Response ◽  
Stress Resistance ◽  
Oxidative Stress Response ◽  
Compensatory Response ◽  
Lower Survival ◽  
Physiological Adaptations ◽  
Altered Regulation

AbstractIn this study, VISA cells carrying vraS and/or graR mutations were shown to be more resistant to oxidative stress. Caenorhabditis elegans infected with these strains in turn demonstrated lower survival. Altered regulation in oxidative stress response and virulence appears to be physiological adaptations associated with VISA phenotype in the Mu50 lineage.

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