scholarly journals Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization

Marine Drugs ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 209 ◽  
Author(s):  
Dina Rodrigues ◽  
Ana R. Costa-Pinto ◽  
Sérgio Sousa ◽  
Marta W. Vasconcelos ◽  
Manuela M. Pintado ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Chemical Characterization ◽  
Sargassum Muticum ◽  
Sulfated Polysaccharides ◽  
Human Consumption ◽  
Molar Ratios ◽  
Assisted Extraction ◽  
Biological Compounds ◽  
Micro Elements

Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts. Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts. Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2–27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4–2.7 g/100gprotein) characterized both extracts. FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida. No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts. The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.

scholarly journals Chemical Characterization of the Lichen-symbiont Microalga Asterochloris erici and Study of Its Cytostatic Effect on the L929 Murine Fibrosarcoma Cell Line

2021 ◽  
Author(s):  
M. Concepción Matesanz ◽  
Mercedes Villa-Carvajal ◽  
Javier Linares ◽  
Sonia Morante-Zarcero ◽  
Isabel Sierra ◽  
...  
Keyword(s):  
Antitumor Agents ◽  
Chemical Characterization ◽  
Human Consumption ◽  
Cytostatic Effect ◽  
In Vitro Biocompatibility ◽  
Murine Fibrosarcoma ◽  
Terrestrial Lichen

New resources of food, pharmaceuticals or biotechnological products are needed. The huge biodiversity of aero-terrestrial lichen-symbiont microalgae remains unexplored. Viability of these for human consumption demands the demonstration of the absence of toxic effects. In vitro biocompatibility of crude homogenates of axenic microalga Asterochloris erici, symbiotic in the lichen Cladonia cristatella, was analyzed after treatment of cultured L929 fibroblasts with different doses of microalgal homogenates. The results show that crude homogenates of A. erici do not induce fibroblast cytotoxicity but seem to have some cytostatic effect inducing slight cell cycle alterations and intracellular reactive oxygen species (ROS) increase at the highest dose. Carotenoid analysis demonstrates high content of lutein, a xanthophyll with antioxidant and cytostatic properties in vivo. These findings confirm that Asterochloris erici can be considered suitable for the development of alimentary or pharmaceutical applications. The cytostatic effects should be further investigated for antitumor agents.

scholarly journals Agronomic and chemical characterization of soybean genotypes for human consumption

2002 ◽  
Vol 2 (4) ◽  
pp. 599-608 ◽  
Author(s):  
D. Destro ◽  
Z.F.S. Miranda ◽  
A.L. Martins ◽  
J.M.G. Mandarino ◽  
L.A. Miranda ◽  
...  
Keyword(s):  
Chemical Characterization ◽  
Human Consumption ◽  
Soybean Genotypes
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