scholarly journals Research Progress of Metabolomics in Asthma

Metabolites ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 567
Author(s):  
Chao Wang ◽  
Shengyu Jiang ◽  
Siyu Zhang ◽  
Zhuoer Ouyang ◽  
Guoqiang Wang ◽  
...  
Keyword(s):  
Immune Response ◽  
Metabolic Regulation ◽  
Exhaled Breath Condensate ◽  
State Regulation ◽  
Research Progress ◽  
Exhaled Breath ◽  
Inflammatory Immune Response ◽  
The Difference ◽  
The Relationship ◽  
Breath Condensate

Asthma is a highly heterogeneous disease, but the pathogenesis of asthma is still unclear. It is well known that the airway inflammatory immune response is the pathological basis of asthma. Metabolomics is a systems biology method to analyze the difference of low molecular weight metabolites (<1.5 kDa) and explore the relationship between metabolic small molecules and pathophysiological changes of the organisms. The functional interdependence between immune response and metabolic regulation is one of the cores of the body’s steady-state regulation, and its dysfunction will lead to a series of metabolic disorders. The signal transduction effect of specific metabolites may affect the occurrence of the airway inflammatory immune response, which may be closely related to the pathogenesis of asthma. Emerging metabolomic analysis may provide insights into the pathogenesis and diagnosis of asthma. The review aims to analyze the changes of metabolites in blood/serum/plasma, urine, lung tissue, and exhaled breath condensate (EBC) samples, and further reveals the potential pathogenesis of asthma according to the disordered metabolic pathways.

scholarly journals Investigating the relationship between breath aerosol size and exhaled breath condensate (EBC) metabolomic content

2020 ◽  
Vol 14 (4) ◽  
pp. 047104
Author(s):  
Alexander J Schmidt ◽  
Eva Borras ◽  
Nicholas J Kenyon ◽  
Cristina E Davis
Keyword(s):  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
The Relationship ◽  
Breath Condensate

scholarly journals Investigating the relationship between particulate matter and inflammatory biomarkers of exhaled breath condensate and blood in healthy young adults

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Morteza Seifi ◽  
Noushin Rastkari ◽  
Mohammad Sadegh Hassanvand ◽  
Kazem Naddafi ◽  
Ramin Nabizadeh ◽  
...  
Keyword(s):  
Young Adults ◽  
Particulate Matter ◽  
Interleukin 6 ◽  
Exhaled Breath Condensate ◽  
Inflammatory Biomarkers ◽  
P Value ◽  
Exhaled Breath ◽  
Exhaled Air ◽  
The Relationship ◽  
Breath Condensate

AbstractInflammatory biomarkers in exhaled breath condensate (EBC) are measured to estimate the effects of air pollution on humans. The present study was conducted to investigate the relationship between particulate matter and inflammatory biomarkers in blood plasma and exhaled air in young adults. The obtained results were compared in two periods; i.e., winter and summer. GRIMM Dust Monitors were used to measure PM10, PM2.5, and PM1 in indoor and outdoor air. A total of 40 healthy young adults exhaling air condensate were collected. Then, biomarkers of interleukin-6 (IL-6), Nitrosothiols (RS-NOs), and Tumor necrosis factor-soluble receptor-II (sTNFRII) were measured by 96 wells method ELISA and commercial kits (HS600B R&D Kit and ALX-850–037-KI01) in EBC while interleukin-6 (IL-6), sTNFRII and White Blood Cell (WBC) were measured in blood plasma in two periods of February 2013 (winter) and May 2013 (summer). Significant association was found between particulate matter and the white blood cell count (p < 0.001), as well as plasma sTNFRII levels (p-value = 0.001). No significant relationship was found between particulate matter with RS-NOs (p = 0.128), EBC RSNOs (p-value = 0.128), and plasma IL-6 (p-value = 0.167). In addition, there was no significant relationship between interleukin-6 of exhaled air with interleukin-6 of plasma (p-value < 0.792 in the first period and < 0.890 in the second period). sTNFRII was not detected in EBC. Considering the direct effect between increasing some biomarkers in blood and EBC and particulate matter, it is concluded that air pollution causes this increasing.

scholarly journals MiRNA Expression Profile in the Airways Is Altered during Pulmonary Exacerbation in Children with Cystic Fibrosis—A Preliminary Report

2020 ◽  
Vol 9 (6) ◽  
pp. 1887
Author(s):  
Zuzanna Stachowiak ◽  
Irena Wojsyk-Banaszak ◽  
Katarzyna Jończyk-Potoczna ◽  
Beata Narożna ◽  
Wojciech Langwiński ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Immune Response ◽  
Expression Profile ◽  
Mirna Expression ◽  
Pediatric Patients ◽  
Exhaled Breath Condensate ◽  
Mirna Expression Profile ◽  
Exhaled Breath ◽  
Pulmonary Exacerbation ◽  
Breath Condensate

MicroRNAs are small non-coding RNAs that regulate immune response and inflammation. We assumed that miRNAs may be involved in the immune response during cystic fibrosis pulmonary exacerbations (CFPE) and that altered expression profile in the airways and blood may underlie clinical outcomes in CF pediatric patients. Methods: We included 30 pediatric patients diagnosed with cystic fibrosis. The biologic material (blood, sputum, exhaled breath condensate) was collected during pulmonary exacerbation and in stable condition. The miRNA expression profile from blood and sputum (n = 6) was done using the next-generation sequencing. For validation, selected four miRNAs were analyzed by qPCR in exosomes from sputum supernatant and exhaled breath condensate (n = 24). NGS analysis was done in Base Space, correlations of gene expression with clinical data were done in Statistica. Results: The miRNA profiling showed that four miRNAs (miR-223, miR-451a, miR-27b-3p, miR-486-5p) were significantly altered during pulmonary exacerbation in CF patients in sputum but did not differ significantly in blood. MiRNA differently expressed in exhaled breath condensate (EBC) and sputum showed correlation with clinical parameters in CFPE. Conclusion: MiRNA expression profile changes in the airways during pulmonary exacerbation in CF pediatric patients. We suggest that miRNA alterations during CFPE are restricted to the airways and strongly correlate with clinical outcome.

scholarly journals Variability in Measures of Exhaled Breath Na+, Influence of pulmonary Blood Flow and salivary Na+

2010 ◽  
Vol 4 ◽  
pp. CCRPM.S4718 ◽  
Author(s):  
Courtney M. Wheatley ◽  
Nicholas A. Cassuto ◽  
William T. Foxx-Lupo ◽  
Eric M. Snyder
Keyword(s):  
Blood Flow ◽  
Pulmonary Blood Flow ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Female Age ◽  
Marked Variability ◽  
Coefficients Of Variation ◽  
Within Subjects ◽  
The Relationship ◽  
Breath Condensate

The assessment of inflammatory markers and ions in exhaled breath condensate (EBC) is being utilized more frequently in diseases such as asthma and cystic fibrosis with marked variability in EBC measures, including those of exhaled Na+. We sought to determine if variability in exhaled Na+ was due to differences in pulmonary blood flow (PBF) or Na+ in the mouth (salivary Na+). We measured exhaled Na+ three times with coinciding sampling of salivary Na+ and assessment of PBF (using acetylene rebreathing) in 13 healthy subjects (54% female, age = 27 ± 7 yrs., ht. = 172 ± 10 cm, wt. = 70 ± 21 kg, BMI = 22 ± 7 kg/m2 mean ± SD). Exhaled Na+ averaged 2.7 ± 1.2 mmol/l, and salivary Na+ averaged 5.51 ± 4.58 mmol/l. The coefficients of variation across all three measures in all 13 subjects averaged 30% for exhaled Na+ and 83% for salivary Na+, within subjects the variability across the three measures averaged 30% for exhaled Na+ and 38% for salivary Na+. Across all three measures in all 13 subjects the relationship between PBF and exhaled Na+ averaged 0.027 ( P = 0.87), and the relationship between salivary Na+ and exhaled Na+ concentrations averaged 0.59 ( P = 0.001). Also, we sought to determine the relationship between exhaled Na+ and serum Na+ in an addition 20 subjects. There was a moderate and significant relationship between serum Na+ and exhaled Na+ (r = 0.37, P = 0.04). These findings suggest there that the variability in exhaled Na+ is caused, at least in part, by droplet formation from within the mouth as turbulent air passes through and that there is a flux of ions from the pulmonary blood into the airways.

scholarly journals Clinical value of serum and exhaled breath condensate inflammatory factor IL-11 levels in non-small cell lung cancer: Clinical value of IL-11 in non-small cell lung cancer

2021 ◽  
pp. 172460082110235
Author(s):  
Jinnan Wu ◽  
Jinliang Chen ◽  
Xuedong Lv ◽  
Qichang Yang ◽  
Sumei Yao ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Carcinoembryonic Antigen ◽  
Exhaled Breath Condensate ◽  
Small Cell ◽  
Exhaled Breath ◽  
Small Cell Lung ◽  
Clinical Value ◽  
The Relationship ◽  
Breath Condensate

Objective: Our study aimed to observe and evaluate the clinical value of interleukin (IL)-11 in the serum and exhaled breath condensate of patients with non-small cell lung cancer (NSCLC). Methods: A total of 91 patients with NSCLC and 72 healthy volunteers were included in this study. IL-11 concentration was determined by ELISA, and the relationship between IL-11 expression in serum and exhaled breath condensate specimens, and the clinicopathological characteristics of patients with NSCLC were analyzed. The relationship between serum IL-11 expression and traditional tumor markers and inflammation indicators of NSCLC was also analyzed. The correlation between serum IL-11 and exhaled breath condensate IL-11 level was determined. The receiver operating characteristic curve was used to evaluate the diagnostic value of IL-11 and carcinoembryonic antigen single and combined detection for NSCLC. The published data from online databases were used to analyze the relationship between the expression of IL-11 and the prognosis of NSCLC. Results: IL-11 concentration in serum and exhaled breath condensate specimens of patients with NSCLC were significantly increased. IL-11 expression was positively correlated with lymph node metastasis, distant metastasis, tumor node metastasis stage, and tumor differentiation degree of NSCLC. The expression of IL-11 in serum was positively correlated with that in exhaled breath condensate specimens. IL-11 expression was closely related to that of neutrophil-to-lymphocyte ratio and carcinoembryonic antigen. The combination of serum IL-11 with exhaled breath condensate IL-11 and carcinoembryonic antigen showed significantly higher diagnostic value than any one marker alone. Besides, the high IL-11 expression was closely related to the poor prognosis of NSCLC. Conclusion: IL-11 can be used as a potential diagnostic and prognostic biomarker for NSCLC.

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