scholarly journals Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis

Metabolites ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 780
Author(s):  
Keke Qi ◽  
Liutian Wu ◽  
Chengyuan Liu ◽  
Yang Pan
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Disease Diagnosis ◽  
Ambient Mass Spectrometry ◽  
Ambient Conditions ◽  
Metabolite Analysis ◽  
Tissue Sections ◽  
Model Research ◽  
One Step

Ambient mass spectrometry imaging (AMSI) has attracted much attention in recent years. As a kind of unlabeled molecular imaging technique, AMSI can enable in situ visualization of a large number of compounds in biological tissue sections in ambient conditions. In this review, the developments of various AMSI techniques are discussed according to one-step and two-step ionization strategies. In addition, recent applications of AMSI for lipid and metabolite analysis (from 2016 to 2021) in disease diagnosis, animal model research, plant science, drug metabolism and toxicology research, etc., are summarized. Finally, further perspectives of AMSI in spatial resolution, sensitivity, quantitative ability, convenience and software development are proposed.

In Situ Hydrogel Conditioning of Tissue Samples To Enhance the Drug’s Sensitivity in Ambient Mass Spectrometry Imaging

2017 ◽  
Vol 89 (12) ◽  
pp. 6318-6323 ◽  
Author(s):  
Xiaowei Song ◽  
Zhigang Luo ◽  
Xin Li ◽  
Tiegang Li ◽  
Zhonghua Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Ambient Mass Spectrometry ◽  
Tissue Samples ◽  
In Situ Hydrogel

scholarly journals In situ characterisation of phytohormones from wounded Arabidopsis leaves using desorption electrospray ionisation mass spectrometry imaging

The Analyst ◽  
2021 ◽  
Author(s):  
Chao Zhang ◽  
Asta Žukauskaitė ◽  
Ivan Petřík ◽  
Aleš Pěnčík ◽  
Martin Hönig ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Plant Development ◽  
Plant Hormones ◽  
Stress Responses ◽  
Mass Spectrometry Imaging ◽  
Signalling Molecules ◽  
Ionisation Mass Spectrometry ◽  
Endogenous Regulators ◽  
Ionisation Mass

Phytohormones (plant hormones) are a group of small signalling molecules that act as important endogenous regulators in the plant development and stress responses. Previous research has identified phytohormone species, jasmonates,...

scholarly journals Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Tyler J. Mason ◽  
Harmonie M. Bettenhausen ◽  
Jacqueline M. Chaparro ◽  
Mark E. Uchanski ◽  
Jessica E. Prenni
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Direct Analysis ◽  
Quality Characteristics ◽  
Ambient Mass Spectrometry ◽  
Postharvest Quality ◽  
Ionization Mass ◽  
Ambient Conditions ◽  
Assess Quality

AbstractHorticulturists are interested in evaluating how cultivar, environment, or production system inputs can affect postharvest quality. Ambient mass spectrometry approaches enable analysis of minimally processed samples under ambient conditions and offer an attractive high-throughput alternative for assessing quality characteristics in plant products. Here, we evaluate direct analysis in real time (DART-MS) mass spectrometry and rapid evaporative ionization-mass spectrometry (REIMS) to assess quality characteristics in various pepper (Capsicum annuum L.) cultivars. DART-MS exhibited the ability to discriminate between pod colors and pungency based on chemical fingerprints, while REIMS could distinguish pepper market class (e.g., bell, lunchbox, and popper). Furthermore, DART-MS analysis resulted in the putative detection of important bioactive compounds in human diet such as vitamin C, p-coumaric acid, and capsaicin. The results of this study demonstrate the potential for these approaches as accessible and reliable tools for high throughput screening of pepper quality.

scholarly journals Exemplifying the Screening Power of Mass Spectrometry Imaging over Label-Based Technologies for Simultaneous Monitoring of Drug and Metabolite Distributions in Tissue Sections

2015 ◽  
Vol 21 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Richard J. A. Goodwin ◽  
Anna Nilsson ◽  
C. Logan Mackay ◽  
John G. Swales ◽  
Maria K. Johansson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Cyclic Peptide ◽  
Analysis Data ◽  
Resolving Power ◽  
High Mass ◽  
Whole Body ◽  
Ion Cyclotron Resonance ◽  
Label Free ◽  
Tissue Sections

Mass spectrometry imaging (MSI) provides pharmaceutical researchers with a suite of technologies to screen and assess compound distributions and relative abundances directly from tissue sections and offer insight into drug discovery–applicable queries such as blood-brain barrier access, tumor penetration/retention, and compound toxicity related to drug retention in specific organs/cell types. Label-free MSI offers advantages over label-based assays, such as quantitative whole-body autoradiography (QWBA), in the ability to simultaneously differentiate and monitor both drug and drug metabolites. Such discrimination is not possible by label-based assays if a drug metabolite still contains the radiolabel. Here, we present data exemplifying the advantages of MSI analysis. Data of the distribution of AZD2820, a therapeutic cyclic peptide, are related to corresponding QWBA data. Distribution of AZD2820 and two metabolites is achieved by MSI, which [14C]AZD2820 QWBA fails to differentiate. Furthermore, the high mass-resolving power of Fourier transform ion cyclotron resonance MS is used to separate closely associated ions.

Mass spectrometry imaging of pharmacological compounds in tissue sections

Bioanalysis ◽  
2010 ◽  
Vol 2 (2) ◽  
pp. 279-293 ◽  
Author(s):  
Richard JA Goodwin ◽  
Andrew R Pitt
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Tissue Sections
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