In situ biomarker discovery and label-free molecular histopathological diagnosis of lung cancer by ambient mass spectrometry imaging

Ambient mass spectrometry imaging (AMSI) has attracted much attention in recent years. As a kind of unlabeled molecular imaging technique, AMSI can enable in situ visualization of a large number of compounds in biological tissue sections in ambient conditions. In this review, the developments of various AMSI techniques are discussed according to one-step and two-step ionization strategies. In addition, recent applications of AMSI for lipid and metabolite analysis (from 2016 to 2021) in disease diagnosis, animal model research, plant science, drug metabolism and toxicology research, etc., are summarized. Finally, further perspectives of AMSI in spatial resolution, sensitivity, quantitative ability, convenience and software development are proposed.

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In situ characterisation of phytohormones from wounded Arabidopsis leaves using desorption electrospray ionisation mass spectrometry imaging

The Analyst ◽

10.1039/d0an02118k ◽

2021 ◽

Author(s):

Chao Zhang ◽

Asta Žukauskaitė ◽

Ivan Petřík ◽

Aleš Pěnčík ◽

Martin Hönig ◽

...

Keyword(s):

Mass Spectrometry ◽

Plant Development ◽

Plant Hormones ◽

Stress Responses ◽

Mass Spectrometry Imaging ◽

Signalling Molecules ◽

Ionisation Mass Spectrometry ◽

Endogenous Regulators ◽

Ionisation Mass

Phytohormones (plant hormones) are a group of small signalling molecules that act as important endogenous regulators in the plant development and stress responses. Previous research has identified phytohormone species, jasmonates,...

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Mass spectrometry imaging reveals in situ behaviors of multiple components in aerosol particles

Angewandte Chemie International Edition ◽

10.1002/anie.202103874 ◽

2021 ◽

Author(s):

Zongxiu Nie ◽

Yuming Jiang ◽

Jie Sun ◽

Caiqiao Xiong ◽

Huihui Liu ◽

...

Keyword(s):

Mass Spectrometry ◽

Mass Spectrometry Imaging ◽

Aerosol Particles ◽

Multiple Components

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Exemplifying the Screening Power of Mass Spectrometry Imaging over Label-Based Technologies for Simultaneous Monitoring of Drug and Metabolite Distributions in Tissue Sections

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057115623740 ◽

2015 ◽

Vol 21 (2) ◽

pp. 187-193 ◽

Cited By ~ 20

Author(s):

Richard J. A. Goodwin ◽

Anna Nilsson ◽

C. Logan Mackay ◽

John G. Swales ◽

Maria K. Johansson ◽

...

Keyword(s):

Mass Spectrometry ◽

Mass Spectrometry Imaging ◽

Cyclic Peptide ◽

Analysis Data ◽

Resolving Power ◽

High Mass ◽

Whole Body ◽

Ion Cyclotron Resonance ◽

Label Free ◽

Tissue Sections

Mass spectrometry imaging (MSI) provides pharmaceutical researchers with a suite of technologies to screen and assess compound distributions and relative abundances directly from tissue sections and offer insight into drug discovery–applicable queries such as blood-brain barrier access, tumor penetration/retention, and compound toxicity related to drug retention in specific organs/cell types. Label-free MSI offers advantages over label-based assays, such as quantitative whole-body autoradiography (QWBA), in the ability to simultaneously differentiate and monitor both drug and drug metabolites. Such discrimination is not possible by label-based assays if a drug metabolite still contains the radiolabel. Here, we present data exemplifying the advantages of MSI analysis. Data of the distribution of AZD2820, a therapeutic cyclic peptide, are related to corresponding QWBA data. Distribution of AZD2820 and two metabolites is achieved by MSI, which [14C]AZD2820 QWBA fails to differentiate. Furthermore, the high mass-resolving power of Fourier transform ion cyclotron resonance MS is used to separate closely associated ions.

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