scholarly journals A Novel Self-Activated Mechanism for Stable Liquid Transportation Capable of Continuous-Flow and Real-time Microfluidic PCRs

Micromachines ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 350 ◽  
Author(s):  
Di Wu ◽  
Bing Shi ◽  
Bin Li ◽  
Wenming Wu
Keyword(s):  
Mathematical Modeling ◽  
Real Time ◽  
Continuous Flow ◽  
Transport Mechanism ◽  
The Self ◽  
Amplification Efficiency ◽  
Passive Transport ◽  
Flow Rates ◽  
Quartz Capillary ◽  
Passive Pumping

The self-activated micropump capable of velocity-stable transport for both single-phased plug and double-phased droplet through long flow distance inside 3D microchannel is one dream of microfluidic scientists. While several types of passive micropumps have been developed based on different actuation mechanisms, until today, it is still one bottleneck to realize such a satisfied self-activated micropump for the stable delivery of both single and double-phased liquid inside long microchannel (e.g., several meters), due to the lack of innovative mechanism in previous methods. To solve this problem, in this article, we propose a new self-activated pumping mechanism. Herein, an end-opened gas-impermeable quartz capillary is utilized for passive transport. Mechanism of this micropump is systemically studied by both the mathematical modeling and the experimental verifications. Based on the flow assays, it totally confirmed a different pumping principle in this paper, as compared with our previous works. The R 2 value of the overall flow rates inside the 3D microchannel is confirmed as high as 0.999, which is much more homogeneous than other passive pumping formats. Finally, this novel micropump is applied to continuous-flow real-time PCRs (both plug-type and microdroplet-type), with the amplification efficiency reaching 91.5% of the commercial PCR cycler instrument.

scholarly journals A New Self-Activated Micropumping Mechanism Capable of Continuous-Flow and Real-Time PCR Amplification Inside 3D Spiral Microreactor

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 685
Author(s):  
Kangning Wang ◽  
Di Wu ◽  
Wenming Wu
Keyword(s):  
Real Time ◽  
Continuous Flow ◽  
Capillary Tube ◽  
Pcr Amplification ◽  
Amplification Efficiency ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Fluid Resistance ◽  
Long Time ◽  
Quartz Capillary

A self-activated micropump which is capable of stable velocity transport for a liquid to flow a given distance inside a 3D microchannel has been a dream of microfluidic scientists for a long time. A new self-activated pumping mechanism has been proposed in this paper. It is different from the authors’ previous research which relied on the fluid resistance of a quartz capillary tube or end-blocked gas-permeable silicone or a polydimethylsiloxane (PDMS) wall to automate the flow. In this research, an end-open stretched Teflon tube was utilized for passive transport for the first time. A new fluid transmission mode was adopted with the assistance of a cheaper easily accessible oil mixture to achieve stable continuous flow. Finally, this novel micropump has been applied to real-time continuous-flow polymerase chain reactions (PCRs), with an amplification efficiency similar to that of a commercial PCR cycler instrument.

A novel mechanism for user-friendly and self-activated microdroplet generation capable of programmable control

The Analyst ◽  
2018 ◽  
Vol 143 (16) ◽  
pp. 3798-3807 ◽  
Author(s):  
Yangyang Jiang ◽  
Lin Du ◽  
Yuanming Li ◽  
Quanquan Mu ◽  
Zhongxu Cui ◽  
...  
Keyword(s):  
Real Time ◽  
Continuous Flow ◽  
Transport Mechanism ◽  
The Real ◽  
Spiral Microchannel ◽  
User Friendly

The real-time continuous-flow PCR inside a 3D spiral microchannel is realized by a novel self-activated microdroplet generation/transport mechanism.

Wearable microfluidics: fabric-based digital droplet flowmetry for perspiration analysis

Lab on a Chip ◽  
2017 ◽  
Vol 17 (5) ◽  
pp. 926-935 ◽  
Author(s):  
Yahui Yang ◽  
Siyuan Xing ◽  
Zecong Fang ◽  
Ruya Li ◽  
Helen Koo ◽  
...  
Keyword(s):  
Real Time ◽  
Continuous Flow ◽  
Time Measurement ◽  
Flow Rates ◽  
Microfluidic Platform ◽  
Textile Materials ◽  
Real Time Measurement ◽  
First Time ◽  
Electronic Counting

Here we show for the first time a wearable microfluidic platform, named as digital droplet flowmetry (DDF), implemented on all-textile materials for real-time measurement of perspiration rates, via converting continuous flow into discrete droplets with identical volumes and assessing the flow rates by an electronic counting circuitry.

scholarly journals A Continuous-Flow Gas Interface of a Thermal/Optical Analyzer With 14C AMS for Source Apportionment of Atmospheric Aerosols

Radiocarbon ◽  
2016 ◽  
Vol 59 (3) ◽  
pp. 921-932 ◽  
Author(s):  
Konstantinos Agrios ◽  
Gary Salazar ◽  
Sönke Szidat
Keyword(s):  
Real Time ◽  
Mass Flow ◽  
Atmospheric Aerosols ◽  
Continuous Flow ◽  
Ion Source ◽  
Flow Rates ◽  
Real Time Analysis ◽  
Accelerator Mass Spectrometer ◽  
Carbon Mass ◽  
Mass Flow Rates

AbstractThis article reports on the performance of a continuous-flow interface for CO2 gas feeding into the ion source of a 200-kV accelerator mass spectrometer (AMS) in splitless mode. Distinct CO2 fractions and subfractions produced by a commercial Sunset OC/EC (organic carbon/elemental carbon) analyzer from ambient atmospheric aerosols filters are injected into the source and then analyzed by their 14C/12C ratio in real time. New features are revealed from organic aerosol subfractions, which thermally desorb close to each other and are only visible by real-time analysis. An optimized setup for this purpose is presented for the measurement of CO2 amounts from 3 to 45 μg C. Efficiencies of 4.5–8.0% for the formation of C- ions from CO2 are obtained for sample masses of 5–10 μg C and carbon mass flow rates below 2 µg min–1. However, this ionization efficiency is substantially suppressed at high carbon mass flow rates. The potential of this method for a refined apportionment of aerosol sources is demonstrated with ambient filter samples.

scholarly journals Advanced Real‐Time Process Analytics for Multistep Synthesis in Continuous Flow**

2021 ◽  
Author(s):  
Peter Sagmeister ◽  
René Lebl ◽  
Ismael Castillo ◽  
Jakob Rehrl ◽  
Julia Kruisz ◽  
...  
Keyword(s):  
Real Time ◽  
Continuous Flow ◽  
Time Process ◽  
Process Analytics ◽  
Multistep Synthesis

scholarly journals Discriminating the eight genotypes of the porcine circovirus type 2 with TaqMan-based real-time PCR

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Ellen Kathrin Link ◽  
Matthias Eddicks ◽  
Liangliang Nan ◽  
Mathias Ritzmann ◽  
Gerd Sutter ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Diagnostic Sensitivity ◽  
Locked Nucleic Acid ◽  
Porcine Circovirus ◽  
Amplification Efficiency ◽  
Pcr Assays

Abstract Background The porcine circovirus type 2 (PCV2) is divided into eight genotypes including the previously described genotypes PCV2a to PCV2f and the two new genotypes PCV2g and PCV2h. PCV2 genotyping has become an important task in molecular epidemiology and to advance research on the prophylaxis and pathogenesis of PCV2 associated diseases. Standard genotyping of PCV2 is based on the sequencing of the viral genome or at least of the open reading frame 2. Although, the circovirus genome is small, classical sequencing is time consuming, expensive, less sensitive and less compatible with mass testing compared with modern real-time PCR assays. Here we report about a new PCV2 genotyping method using qPCR. Methods Based on the analysis of several hundred PCV2 full genome sequences, we identified PCV2 genotype specific sequences or single-nucleotide polymorphisms. We designed six TaqMan PCR assays that are specific for single genotypes PCV2a to PCV2f and two qPCRs targeting two genotypes simultaneously (PCV2g/PCV2d and PCV2h/PCV2c). To improve specific binding of oligonucleotide primers and TaqMan probes, we used locked nucleic acid technology. We evaluated amplification efficiency, diagnostic sensitivity and tested assay specificity for the respective genotypes. Results All eight PCV2 genotype specific qPCRs demonstrated appropriate amplification efficiencies between 91 and 97%. Testing samples from an epidemiological field study demonstrated a diagnostic sensitivity of the respective genotype specific qPCR that was comparable to a highly sensitive pan-PCV2 qPCR system. Genotype specificity of most qPCRs was excellent. Limited unspecific signals were obtained when a high viral load of PCV2b was tested with qPCRs targeting PCV2d or PCV2g. The same was true for the PCV2a specific qPCR when high copy numbers of PCV2d were tested. The qPCR targeting PCV2h/PCV2c showed some minor cross-reaction with PCV2d, PCV2f and PCV2g. Conclusion Genotyping of PCV2 is important for routine diagnosis as well as for epidemiological studies. The introduced genotyping qPCR system is ideal for mass testing and should be a valuable complement to PCV2 sequencing, especially in the case of simultaneous infections with multiple PCV2 genotypes, subclinically infected animals or research studies that require large sample numbers.

scholarly journals Advanced Real‐Time Process Analytics for Multistep Synthesis in Continuous Flow**

2021 ◽  
Author(s):  
Peter Sagmeister ◽  
René Lebl ◽  
Ismael Castillo ◽  
Jakob Rehrl ◽  
Julia Kruisz ◽  
...  
Keyword(s):  
Real Time ◽  
Continuous Flow ◽  
Time Process ◽  
Process Analytics ◽  
Multistep Synthesis

scholarly journals Ferro-self-assembly: magnetic and electrochemical adaptation of a multiresponsive zwitterionic metalloamphiphile showing a shape-hysteresis effect

2021 ◽  
Vol 12 (1) ◽  
pp. 270-281
Author(s):  
Stefan Bitter ◽  
Moritz Schlötter ◽  
Markus Schilling ◽  
Marina Krumova ◽  
Sebastian Polarz ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Real Time ◽  
Self Assembly ◽  
Hysteresis Effect ◽  
The Self ◽  
Self Organization ◽  
Stimuli Responsive ◽  
Optical Birefringence

The self-organization properties of a stimuli responsive amphiphile can be altered by subjecting the paramagnetic oxidized form to a magnetic field of 0.8 T and monitored in real time by coupling optical birefringence with dynamic light scattering.

scholarly journals Computation of transient flow rates in passive pumping micro-fluidic systems

Lab on a Chip ◽  
2009 ◽  
Vol 9 (1) ◽  
pp. 107-114 ◽  
Author(s):  
I-Jane Chen ◽  
Eugene C. Eckstein ◽  
Ernő Lindner
Keyword(s):  
Transient Flow ◽  
Flow Rates ◽  
Passive Pumping
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