Biocontrol of Soft Rot Caused by Pectobacterium odoriferum with Bacteriophage phiPccP-1 in Kimchi Cabbage

Pectobacterium odoriferum has recently emerged as a widely infective and destructive pathogen causing soft-rot disease in various vegetables. Bacteriophage phiPccP-1 isolated from Pyeongchang, South Korea, showed lytic activity against P. odoriferum Pco14 and two other Pectobacterium species. The transmission electron microscopy and genome phylograms revealed that phiPccP-1 belongs to the Unyawovirus genus, Studiervirinae subfamily of the Autographivirinae family. Genome comparison showed that its 40,487 bp double-stranded DNA genome shares significant similarity with Pectobacterium phage DU_PP_II with the identity reaching 98% of the genome. The phiPccP-1 application significantly inhibited the development of soft-rot disease in the mature leaves of the harvested Kimchi cabbage up to 48 h after Pco14 inoculation compared to the untreated leaves, suggesting that phiPccP-1 can protect Kimchi cabbage from soft-rot disease after harvest. Remarkably, bioassays with phiPccP-1 in Kimchi cabbage seedlings grown in the growth chamber successfully demonstrated its prophylactic and therapeutic potential in the control of bacterial soft-rot disease in Kimchi cabbage. These results indicate that bacteriophage phiPccP-1 can be used as a potential biological agent for controlling soft rot disease in Kimchi cabbage.

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Screening, Identification and Efficacy Evaluation of Antagonistic Bacteria for Biocontrol of Soft Rot Disease Caused by Dickeya zeae

Microorganisms ◽

10.3390/microorganisms8050697 ◽

2020 ◽

Vol 8 (5) ◽

pp. 697 ◽

Cited By ~ 1

Author(s):

Jieling Li ◽

Ming Hu ◽

Yang Xue ◽

Xia Chen ◽

Guangtao Lu ◽

...

Keyword(s):

Antimicrobial Activity ◽

Prevention And Control ◽

Pathogenic Bacteria ◽

Soft Rot ◽

Antagonistic Bacteria ◽

Bacterial Soft Rot ◽

Soft Rot Disease ◽

Wide Range ◽

Rot Disease ◽

And Control

Dickeya zeae is the causal agent of bacterial soft rot disease, with a wide range of hosts all over the world. At present, chemical agents, especially agricultural antibiotics, are commonly used in the prevention and control of bacterial soft rot, causing the emergence of resistant pathogens and therefore increasing the difficulty of disease prevention and control. This study aims to provide a safer and more effective biocontrol method for soft rot disease caused by D. zeae. The spot-on-lawn assay was used to screen antagonistic bacteria, and three strains including SC3, SC11 and 3-10 revealed strong antagonistic effects and were identified as Pseudomonas fluorescens, P. parafulva and Bacillus velezensis, respectively, using multi-locus sequence analysis (MLSA) based on the sequences of 16S rRNA and other housekeeping genes. In vitro antimicrobial activity showed that two Pseudomonas strains SC3 and SC11 were only antagonistic to some pathogenic bacteria, while strain 3-10 had broad-spectrum antimicrobial activity on both pathogenic bacteria and fungi. Evaluation of control efficacy in greenhouse trials showed that they all restrained the occurrence and development of soft rot disease caused by D. zeae MS2 or EC1. Among them, strain SC3 had the most impressive biocontrol efficacy on alleviating the soft rot symptoms on both monocotyledonous and dicotyledonous hosts, and strain 3-10 additionally reduced the occurrence of banana wilt disease caused by Fusarium oxysporum f. sp. cubensis. This is the first report of P. fluorescens, P. parafulva and B. velezensis as potential bio-reagents on controlling soft rot disease caused by D. zeae.

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Plant ferredoxin-like protein enhances resistance to bacterial soft rot disease through PAMP-triggered immunity in Arabidopsis thaliana

European Journal of Plant Pathology ◽

10.1007/s10658-014-0471-0 ◽

2014 ◽

Vol 140 (2) ◽

pp. 377-384 ◽

Cited By ~ 8

Author(s):

Yu-Han Su ◽

Chuan-Yu Hong ◽

Yi-Hsien Lin

Keyword(s):

Arabidopsis Thaliana ◽

Soft Rot ◽

Bacterial Soft Rot ◽

Soft Rot Disease ◽

Rot Disease

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First Report of Bacterial Soft Rot Disease on Mammillaria mystax Caused by Enterobacter cloacae subsp. dissolvens in Mexico

Plant Disease ◽

10.1094/pdis-08-19-1662-pdn ◽

2020 ◽

Vol 104 (5) ◽

pp. 1536-1536

Author(s):

Guadalupe Reyes-García ◽

Santo Ángel Ortega-Acosta ◽

Francisco Palemón-Alberto ◽

Yanet Romero Ramírez ◽

Jeiry Toribio-Jiménez ◽

...

Keyword(s):

Enterobacter Cloacae ◽

Soft Rot ◽

Bacterial Soft Rot ◽

First Report ◽

Soft Rot Disease ◽

Rot Disease

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Characterization of Dickeya fangzhongdai causing bacterial soft rot disease on Dendrobium nobile in India

European Journal of Plant Pathology ◽

10.1007/s10658-020-02094-7 ◽

2020 ◽

Vol 158 (3) ◽

pp. 773-780

Author(s):

A. Balamurugan ◽

A. Kumar ◽

K. Sakthivel ◽

M. Ashajyothi ◽

Kuleshwar Prasad Sahu ◽

...

Keyword(s):

Soft Rot ◽

Bacterial Soft Rot ◽

Dendrobium Nobile ◽

Soft Rot Disease ◽

Rot Disease

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001 Establishment of Methods in Evaluating the Susceptibility of Chinese Cabbage (Brassica campestris spp.) to Soft Rot Disease by Erwinia

HortScience ◽

10.21273/hortsci.34.3.440 ◽

1999 ◽

Vol 34 (3) ◽

pp. 440-441

Author(s):

Won Jun ◽

Soo-Seong Lee ◽

Jongkee Kim

Keyword(s):

Chinese Cabbage ◽

Large Scale ◽

Erwinia Carotovora ◽

Soft Rot ◽

Experimental Plant ◽

Bacterial Soft Rot ◽

Breeding Lines ◽

Inoculation Methods ◽

Soft Rot Disease ◽

Rot Disease

Three inoculation methods, including cutting of a leaf, drenching, and point inoculation, were compared in an effort to screen the susceptibility of Chinese cabbage to soft rot disease caused by Erwinia carotovora subsp. carotovora. Three- to 4-week-old seedlings from 10 lines of cabbage with 16-h-old bacterial culture were routinely used. Inoculated seedlings were kept at 25.0 ± 10.0 °C for 48 h with saturated water vapor using a plastic tunnel in a greenhouse. Sixty-day-old mature plants were produced and inoculated in a greenhouse. Severity of symptoms, which were observed from percentage of plant infected was scored as from 1 to 9, representing resistant to susceptible, respectively. The correlation between seedlings and mature plants from ten lines was evaluated among the three different inoculation methods. Point inoculation gave the most significant correlation (r = 0.843, P < 0.05) between seedlings and mature plants. A good correlation was also observed between point inoculation of seedlings and drenching of mature plants (r = 0.609, P < 0.05). Cutting of a leaf of seedlings was also correlated with point inoculation of mature plants (r = 0.609, P < 0.05). This method provides the advantage of being able to keep the experimental plant alive, as only one leaf is detached. The point inoculation method is simple and relatively sensitive, so it could be used for large-scale screening for this bacterial soft rot disease. From three different evaluation assays, it was concluded that the breeding lines, C3-28, C3-29 from Cornell Univ. (Geneva, N.Y.) and the cultivar Kweonsim319 were relatively resistant to bacterial soft rot, while the Cornell breeding line CC-25 and the `Rang-no' cultivar were relatively susceptible.

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Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests

Bulletin of the National Research Centre ◽

10.1186/s42269-021-00491-4 ◽

2021 ◽

Vol 45 (1) ◽

Author(s):

Hassan Abd-El-Khair ◽

Tarek G. Abdel-Gaied ◽

Maurice S. Mikhail ◽

Ahmed I. Abdel-Alim ◽

Hamdy I. Seif El-Nasr

Keyword(s):

Potato Tuber ◽

Soft Rot ◽

Pectobacterium Carotovorum ◽

Bacterial Soft Rot ◽

Potato Tubers ◽

Soft Rot Disease ◽

In Vivo Tests ◽

Rot Disease

Abstract Background Several chemical bactericides were applied for controlling soft rot bacteria, Pectobacterium carotovorum subsp. carotovorum, which causes the destructive soft rot disease to many economically important vegetables, but because of their toxic hazards on human and environment became limit. The biocontrol was applied to control many plant pathogens. Therefore, this work is aimed to study the antagonistic activity of bacterial agents, i.e. Bacillus subtilis, Bacillus pumilus, Bacillus megaterium and Pseudomonas fluorescens, and fugal agents, i.e. Trichoderma harzianum, Trichoderma viride and Trichoderma virens, to control bacterial soft rot disease under in vitro and in vivo tests. Results The tested treatments could protect the potato tubers against the development of soft rot. T. viride and T. virens were highly effective in reducing soft rot symptoms on inoculated potato tuber slices, when applied at the same time or 2 h before pathogen inoculation, while B. megaterium and T. harzianum were highly effective when applied at the same time or 2 h after pathogen inoculation. In whole potato tubers technique, B.pumilus highly protected the stored potato tuber under artificially infection conditions, than P. fluorescens, T. harzianum, B. subtilis, T. viride, T. virens and B. megaterium, respectively. Conclusion Application of fungal agents or specify the bacterial species can play an important role in controlling bacterial soft rot disease in vegetables and increase the stored periods of potato tubers under storage conditions without any toxic effects.

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