scholarly journals Mycobacterium microti at the Environment and Wildlife Interface

2021 ◽  
Vol 9 (10) ◽  
pp. 2084
Author(s):  
Valentina Tagliapietra ◽  
Maria Beatrice Boniotti ◽  
Anna Mangeli ◽  
Iyad Karaman ◽  
Giovanni Alborali ◽  
...  
Keyword(s):  
Wild Boar ◽  
Mycobacterium Tuberculosis Complex ◽  
Northern Italy ◽  
Genetic Profile ◽  
Apodemus Flavicollis ◽  
Wild Rodents ◽  
Tuberculosis Complex ◽  
Animal Aggregation ◽  
Water And Soil Samples ◽  
Mycobacterium Microti

An unexpected high presence of Mycobacterium microti in wild boar in Northern Italy (Garda Lake) has been reported since 2003, but the factors contributing to the maintenance of this pathogen are still unclear. In this study, we investigated the presence of M. microti in wild rodents and in water and soil samples collected at wild boar aggregation areas, such as watering holes, with the aim of clarifying their role in M. microti transmission. In total, 8 out of 120 captured animals tested positive for the Mycobacterium tuberculosis complex (MTBC) as assessed by real-time PCR, and six samples were confirmed to be M. microti. A strain with a genetic profile similar to those previously isolated in wild boars in the same area was isolated from one sample. Of the 20 water and 19 mud samples, 3 and 1, respectively, tested positive for the presence of MTBC, and spacer oligotype SB0118 (vole type) was detected in one sample. Our study suggests that wild rodents, in particular Apodemus sylvaticus, Microtus sp. and Apodemus flavicollis, play roles in the maintenance of M. microti infections in wild boar through ingestion or by contact with either infected excreta or a contaminated environment, such as at animal aggregation sites.

scholarly journals Mycobacterium microti Interferes with Bovine Tuberculosis Surveillance

2020 ◽  
Vol 8 (12) ◽  
pp. 1850
Author(s):  
Lorraine Michelet ◽  
Krystel de Cruz ◽  
Jennifer Tambosco ◽  
Sylvie Hénault ◽  
Maria Laura Boschiroli
Keyword(s):  
Bovine Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Skin Testing ◽  
Surveillance Program ◽  
Molecular Tools ◽  
Wild Rodents ◽  
Tuberculosis Complex ◽  
Molecular Tests ◽  
Ifn Γ ◽  
Mycobacterium Microti

Mycobacterium microti, a member of the Mycobacterium tuberculosis complex, was originally described as the cause of tuberculosis in wild rodents. However, in the last few years, an increasing number of cases have been reported in wildlife (wild boars and badgers) and livestock (goat and cattle) in the frame of bovine tuberculosis (bTB) surveillance program, demonstrating the risk of interference with bTB diagnosis in France. In 2019, we detected four cattle infected with M.microti, from three different herds in three different distant regions. For all these cases, ante-mortem diagnosis by the skin test (single intradermal comparative cervical tuberculin (SICCT)) was positive. Confirmation of M.microti infection was based on molecular tests, i.e., specific real-time PCR and spoligotyping. These results highlight a non-negligible risk of interference in the bTB diagnosis system and raise concern about the reliability of diagnostic tests used for bTB surveillance. The use of highly specific tests, like the interferon gamma test (IFN-γ) employed in France or new synthetic specific tuberculins for skin testing could alternatively be used to accurately identify M.bovis (or Mycobacterium caprae) infection at ante-mortem examination. At post-mortem diagnosis, the use of specific molecular tools should be considered to accurately distinguish pathogens within the MTBC and to avoid misleading bTB diagnosis.

Prevalence of serum antibodies against the Mycobacterium tuberculosis complex in wild boar in Campania region, Italy.

2020 ◽  
Vol 66 (1) ◽  
Author(s):  
Valentina Iovane ◽  
Gianmarco Ferrara ◽  
Angela Petruccelli ◽  
Vincenzo Veneziano ◽  
Nicola D’Alessio ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Wild Boar ◽  
Mycobacterium Tuberculosis Complex ◽  
Serum Antibodies ◽  
Tuberculosis Complex ◽  
Campania Region

scholarly journals Exposure of Wild Boar to Mycobacterium tuberculosis Complex in France since 2000 Is Consistent with the Distribution of Bovine Tuberculosis Outbreaks in Cattle

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77842 ◽  
Author(s):  
Céline Richomme ◽  
Mariana Boadella ◽  
Aurélie Courcoul ◽  
Benoît Durand ◽  
Antoine Drapeau ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Wild Boar ◽  
Bovine Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex

scholarly journals Evaluation of Volatile Organic Compounds Obtained from Breath and Feces to Detect Mycobacterium tuberculosis Complex in Wild Boar (Sus scrofa) in Doñana National Park, Spain

Pathogens ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 346 ◽  
Author(s):  
Pauline Nol ◽  
Radu Ionescu ◽  
Tesfalem Geremariam Welearegay ◽  
Jose Angel Barasona ◽  
Joaquin Vicente ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Volatile Organic Compounds ◽  
Wild Boar ◽  
Organic Compounds ◽  
Sus Scrofa ◽  
National Park ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex ◽  
Doñana National Park ◽  
Volatile Organic

The presence of Mycobacterium tuberculosis complex (MTBC) in wild swine, such as in wild boar (Sus scrofa) in Eurasia, is cause for serious concern. Development of accurate, efficient, and noninvasive methods to detect MTBC in wild swine would be highly beneficial to surveillance and disease management efforts in affected populations. Here, we describe the first report of identification of volatile organic compounds (VOC) obtained from the breath and feces of wild boar to distinguish between MTBC-positive and MTBC-negative boar. We analyzed breath and fecal VOC collected from 15 MTBC-positive and 18 MTBC-negative wild boar in Donaña National Park in Southeast Spain. Analyses were divided into three age classes, namely, adults (>2 years), sub-adults (12–24 months), and juveniles (<12 months). We identified significant compounds by applying the two-tailed statistical t-test for two samples assuming unequal variance, with an α value of 0.05. One statistically significant VOC was identified in breath samples from adult wild boar and 14 were identified in breath samples from juvenile wild boar. One statistically significant VOC was identified in fecal samples collected from sub-adult wild boar and three were identified in fecal samples from juvenile wild boar. In addition, discriminant function analysis (DFA) was used to build classification models for MTBC prediction in juvenile animals. Using DFA, we were able to distinguish between MTBC-positive juvenile wild boar and MTBC-negative juvenile wild boar using breath VOC or fecal VOC. Based on our results, further research is warranted and should be performed using larger sample sizes, as well as wild boar from various geographic locations, to verify these compounds as biomarkers for MTBC infection in this species. This new approach to detect MTBC infection in free-ranging wild boar potentially comprises a reliable and efficient screening tool for surveillance in animal populations.

scholarly journals Detection and Molecular Characterization of Mycobacterium microti Isolates in Wild Boar from Northern Italy

2014 ◽  
Vol 52 (8) ◽  
pp. 2834-2843 ◽  
Author(s):  
M. B. Boniotti ◽  
A. Gaffuri ◽  
D. Gelmetti ◽  
S. Tagliabue ◽  
M. Chiari ◽  
...  
Keyword(s):  
Wild Boar ◽  
Molecular Characterization ◽  
Northern Italy ◽  
Mycobacterium Microti

Spatio-Temporal Trends of Iberian Wild Boar Contact with Mycobacterium tuberculosis Complex Detected by ELISA

EcoHealth ◽  
2011 ◽  
Vol 8 (4) ◽  
pp. 478-484 ◽  
Author(s):  
Mariana Boadella ◽  
Pelayo Acevedo ◽  
Joaquín Vicente ◽  
Gregorio Mentaberre ◽  
Ana Balseiro ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Wild Boar ◽  
Mycobacterium Tuberculosis Complex ◽  
Temporal Trends ◽  
Tuberculosis Complex ◽  
Spatio Temporal

scholarly journals Genome structure in the vole bacillus, Mycobacterium microti, a member of the Mycobacterium tuberculosis complex with a low virulence for humans

Microbiology ◽  
2004 ◽  
Vol 150 (5) ◽  
pp. 1519-1527 ◽  
Author(s):  
Cristiane C. Frota ◽  
Debbie M. Hunt ◽  
Roger S. Buxton ◽  
Lisa Rickman ◽  
Jason Hinds ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Genome Structure ◽  
Biochemical Properties ◽  
Extensive Study ◽  
Tuberculosis Complex ◽  
Almost All ◽  
Mycobacterium Microti ◽  
Different Levels ◽  
Immunocompetent Patients

Mycobacterium microti, a member of the Mycobacterium tuberculosis complex, is phylogenetically closely related to M. tuberculosis, differing in a few biochemical properties. However, these species have different levels of virulence in different hosts; most notably M. microti shows lower virulence for humans than M. tuberculosis. This report presents genomic comparisons using DNA microarray analysis for an extensive study of the diversity of M. microti strains. Compared to M. tuberculosis H37Rv, 13 deletions were identified in 12 strains of M. microti, including the regions RD1 to RD10, which are also missing in Mycobacterium bovis BCG. In addition, four new deleted regions, named MiD1, RD1β, MiD2 and MiD3, were identified. DNA sequencing was used to define the extent of most of the deletions in one strain. Although RD1 of M. bovis BCG and M. microti is thought to be crucial for attenuation, in this study, three of the four M. microti strains that were isolated from immunocompetent patients had the RD1 deletion. In fact, only the RD3 deletion was present in all of the strains examined, although deletions RD7, RD8 and MiD1 were found in almost all the M. microti strains. These deletions might therefore have some relation to the different host range of M. microti. It was also noticeable that of the 12 strains studied, only three were identical; these strains were all isolated from immunocompetent humans, suggesting that they could have arisen from a single source. Thus, this study shows that it is difficult to ascribe virulence to any particular pattern of deletion in M. microti.

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