scholarly journals 2-Methylisoborneol (2-MIB) Excretion by Pseudanabaena yagii under Low Temperature

2021 ◽  
Vol 9 (12) ◽  
pp. 2486
Author(s):  
Ju-Yong Jeong ◽  
Sang-Hoon Lee ◽  
Mi-Ra Yun ◽  
Seung-Eun Oh ◽  
Kyong-Hee Lee ◽  
...  
Keyword(s):  
Low Temperature ◽  
Distribution System ◽  
Water Distribution ◽  
Sequence Similarity ◽  
Freshwater Environment ◽  
Phylogenetic Characterization ◽  
Han River ◽  
The North ◽  
Molecular Phylogenetic ◽  
Freshwater System

Outbreaks of 2-methylisoborneol (2-MIB) contamination in drinking water sources cause inconvenient odor issues in the water distribution system. In this study, microscopy-based isolation with physiological and molecular phylogenetic characterization were performed to investigate and characterize the 2-MIB odor producers that caused an odor problem in the freshwater system of the North Han River in the autumn of 2018. A benthic cyanobacterium was isolated from 2-MIB odor-issue freshwater samples and was found to be phylogenetically affiliated with Pseudanabaena yagii (99.66% sequence similarity), which was recorded in South Korea for the first time. The 2-MIB synthesis gene sequences from the odor-issue freshwater samples showed 100% similarity with those in the P. yagii strains. Protein sequences of 2-MIB synthase observed in the genome of the isolated strain showed structural and functional characteristics similar to those observed in other Pseudanabaena species. The 2-MIB production rate increased slowly during mat formation on the vessel wall; however, it rapidly increased after the temperature dropped. The 2-MIB gene was continuously expressed regardless of the temperature changes. These results suggest that the 2-MIB odor issue in the North Han River might be caused by the release of 2-MIB from the mat-forming P. yagii species in a low-temperature freshwater environment.

An Outbreak of Giardiasis and Amoebiasis at a Ski Resort in Sweden

1989 ◽  
Vol 21 (3) ◽  
pp. 143-146 ◽  
Author(s):  
Y. Andersson ◽  
B. de Jong
Keyword(s):  
Drinking Water ◽  
Distribution System ◽  
Giardia Lamblia ◽  
Water Distribution ◽  
Water Reservoir ◽  
Polluted Water ◽  
Double Infection ◽  
Ski Resort ◽  
The North ◽  
Drinking Water Reservoir

A large number of tourists contracted gastroenteritis at the end of December 1986 at a ski resort in the north of Sweden. The outbreak was due to the intrusion of sewage in the drinking water reservoir through a spillway overflow connected to the sewer. Sewage polluted the drinking water. One to three days after drinking the polluted water, at least 3,600 persons of 4,000 at risk contracted gastroenteritis. The attack rate was 90%. Giardia lamblia was isolated from 1,480 persons and Entamoeba histolytica from 106. At least 50 persons contracted a double infection with G. lamblia and E. histolytica. G. lamblia cysts were isolated from two points in the water distribution system.

scholarly journals Chryseobacterium hispanicum sp. nov., isolated from the drinking water distribution system of Sevilla, Spain

2006 ◽  
Vol 56 (7) ◽  
pp. 1589-1592 ◽  
Author(s):  
Virginia Gallego ◽  
Maria Teresa García ◽  
Antonio Ventosa
Keyword(s):  
Drinking Water ◽  
Distribution System ◽  
Water Distribution ◽  
Bacterial Population ◽  
Water Distribution System ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Drinking Water Distribution ◽  
Type Strains ◽  
The 16S Rrna Gene

Strain VP48T was isolated from drinking water during a screening programme to monitor the bacterial population present in the water distribution system of Sevilla (Spain). A polyphasic taxonomic study of the isolate resulted in its identification as a member of the genus Chryseobacterium, members of which are widely distributed in soil, water and clinical sources. However, the 16S rRNA gene sequence similarity values of strain VP48T to the type strains of Chryseobacterium species were 96 % or lower. Furthermore, phenotypic characteristics clearly indicated that the isolate represents a novel Chryseobacterium species, for which the name Chryseobacterium hispanicum sp. nov. is proposed; strain VP48T (=CECT 7129T=CCM 7359T=JCM 13554T) is the type strain. The DNA G+C content of this strain is 34.3 mol%.

scholarly journals Pedobacter aquatilis sp. nov., isolated from drinking water, and emended description of the genus Pedobacter

2006 ◽  
Vol 56 (8) ◽  
pp. 1853-1858 ◽  
Author(s):  
Virginia Gallego ◽  
Maria Teresa García ◽  
Antonio Ventosa
Keyword(s):  
Drinking Water ◽  
Distribution System ◽  
Type Strain ◽  
Water Distribution ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Sequence Comparisons ◽  
Drinking Water Distribution ◽  
Bacterium Strain ◽  
Type Strains

A Gram-negative, rod-shaped, non-spore-forming bacterium (strain AR107T) was isolated from the drinking water distribution system of Seville (Spain). A polyphasic taxonomic study of the isolate resulted in its identification as a member of the genus Pedobacter. On the basis of 16S rRNA gene sequence comparisons, strain AR107T was shown to belong to the phylum Bacteroidetes, being related to members of the genus Pedobacter. It showed 95.2 % sequence similarity with respect to the type strains of Pedobacter heparinus and Pedobacter piscium, and 94.1 % similarity with respect to the type strain of Pedobacter himalayensis. The predominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c), which supports the affiliation of strain AR107T to the genus Pedobacter. The DNA G+C content of this strain was 38 mol%. On the basis of the phenotypic, phylogenetic and genotypic results, strain AR107T represents a novel species, for which the name Pedobacter aquatilis sp. nov. is proposed. The type strain is AR107T (=CCM 7347T=CECT 7114T=JCM 13454T).

scholarly journals Roseomonas aquatica sp. nov., isolated from drinking water

2006 ◽  
Vol 56 (10) ◽  
pp. 2291-2295 ◽  
Author(s):  
Virginia Gallego ◽  
Cristina Sánchez-Porro ◽  
Maria Teresa García ◽  
Antonio Ventosa
Keyword(s):  
Drinking Water ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Distribution System ◽  
Water Distribution ◽  
Sequence Data ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Drinking Water Distribution

Strain TR53T, a Gram-negative, non-motile, non-spore-forming and strictly aerobic coccobacillus, isolated from the drinking water distribution system of Seville, Spain, was subjected to polyphasic taxonomic analysis using a combination of phenotypic, genotypic and phylogenetic features. Phylogenetic analysis of 16S rRNA gene sequences showed that strain TR53T had highest similarity to members of the genus Roseomonas, with sequence similarity values between 95.7 % (to Roseomonas genomospecies 5 strain ATCC 49960) and 94.0 % (to Roseomonas gilardii subsp. rosea ATCC 49956T). On the basis of its phenotypic characteristics, 16S rRNA gene sequence data and DNA G+C content (68.6 mol%), strain TR53T represents a novel species of the genus Roseomonas, for which the name Roseomonas aquatica sp. nov. is proposed. The type strain of Roseomonas aquatica is TR53T (=CECT 7131T=JCM 13556T).

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