scholarly journals Assessment of the best operating conditions in the enzymatic hydrolysis of pretreated bagasse for bagasse ethanol.

2018 ◽  
Author(s):  
Carmen Salvador Pinos ◽  
Adalis Mesa Noval ◽  
Ángel Batallas Merino ◽  
Jonathan Villavicencio ◽  
Layanis Mesa Garriga ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Operating Conditions ◽  
Pretreated Bagasse ◽  
Hydrolysis Of

Sweet Sorghum as Feedstock for Ethanol Production: Enzymatic Hydrolysis of Steam-Pretreated Bagasse

2008 ◽  
Vol 153 (1-3) ◽  
pp. 151-162 ◽  
Author(s):  
Bálint Sipos ◽  
Jutka Réczey ◽  
Zsolt Somorai ◽  
Zsófia Kádár ◽  
Dóra Dienes ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Sweet Sorghum ◽  
Pretreated Bagasse ◽  
Hydrolysis Of

scholarly journals Enzymatic Hydrolysis of Wheat Straw in a Stirred-Tank Reactor

2021 ◽  
Author(s):  
Ejaz Khan
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Reaction Medium ◽  
Operating Conditions ◽  
Stirred Tank ◽  
Atmospheric Conditions ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
Hydrolysis Of ◽  
Initial Conversion

In an attempt to elucidate the effect of some operating conditions on the rate and extent of enzymatic hydrolysis of lignocellulosic materials in a stirred tank reactor, wheat straw was hydrolyzed by mixing with two pitched-blade impellers mounted on a shaft under various atmospheric conditions: static air in headspace, N₂ gas flowing over the surface of the medium, and minimal static air by a lid touching the surface of the medium. The presence of N₂ gas over the reaction medium produced by the highest 6.9 % (w/w) conversion in 36 hours. The initial conversion when N₂ gas flowed in the headspace (2.9 % w/w), and when a lid was used (2.9 % w/w) as compared to conversion in air presence (2.3 % w/w), seems to indicate that the enzyme activity was affected due to oxidation in the presence of air. The observed low conversion yield was probably the result of the non sterile conditions imposed by industrial requirements for the production of biofuel ethanol from agricultural lignocellulosics.

scholarly journals Assessing the Enzymatic Hydrolysis of Salmon Frame Proteins through Different By-Product/Water Ratios and pH Regimes

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3045
Author(s):  
Pedro Valencia ◽  
Silvana Valdivia ◽  
Suleivys Nuñez ◽  
Reza Ovissipour ◽  
Marlene Pinto ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Control Systems ◽  
Ph Control ◽  
Operating Conditions ◽  
Molecular Characteristics ◽  
Degree Of Hydrolysis ◽  
Added Water ◽  
Hydrolysis Of ◽  
By Products ◽  
Water Ratio

The enzymatic hydrolysis of fish by-product proteins is traditionally carried out by mixing ground by-products with water. In addition, pH control is used to avoid pH drops. Higher costs are involved due to the use of pH control systems and the consequent energy cost in the drying stage. This work aimed to evaluate the effect of these conditions on the hydrolysis of salmon frame (SF) proteins, including the SF hydrolysis without added water. SF hydrolysis by subtilisin at 50, 75, and 100% SF under different pH regimes were evaluated by released α-amino (α-NH) groups, total nitrogen, degree of hydrolysis, and estimated peptide chain length (PCL) at 55 °C. The concentration of released α-NH groups was higher in the conditions with less added water. However, the nitrogen recovery decreased from 50 to 24% at 50 and 100% SF, respectively. Changing the SF/water ratio had a more significant effect than changing the pH regime. Estimated PCL changed from 5–7 to 7–9 at 50 and 100% SF, respectively. The operating conditions affected the hydrolysis performance and the molecular characteristics of the hydrolysate.

scholarly journals Enzymatic Hydrolysis of Wheat Straw in a Stirred-Tank Reactor

2021 ◽  
Author(s):  
Ejaz Khan
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Reaction Medium ◽  
Operating Conditions ◽  
Stirred Tank ◽  
Atmospheric Conditions ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
Hydrolysis Of ◽  
Initial Conversion

In an attempt to elucidate the effect of some operating conditions on the rate and extent of enzymatic hydrolysis of lignocellulosic materials in a stirred tank reactor, wheat straw was hydrolyzed by mixing with two pitched-blade impellers mounted on a shaft under various atmospheric conditions: static air in headspace, N₂ gas flowing over the surface of the medium, and minimal static air by a lid touching the surface of the medium. The presence of N₂ gas over the reaction medium produced by the highest 6.9 % (w/w) conversion in 36 hours. The initial conversion when N₂ gas flowed in the headspace (2.9 % w/w), and when a lid was used (2.9 % w/w) as compared to conversion in air presence (2.3 % w/w), seems to indicate that the enzyme activity was affected due to oxidation in the presence of air. The observed low conversion yield was probably the result of the non sterile conditions imposed by industrial requirements for the production of biofuel ethanol from agricultural lignocellulosics.

scholarly journals Effects of Lignin Removal on Substrate Enzymatic Hydrolysis of SPORL-high-pH Pretreated Bagasse

BioResources ◽  
2013 ◽  
Vol 8 (3) ◽  
Author(s):  
Jing Liu ◽  
Xiaolin Luo ◽  
Shilin Cao ◽  
Lihui Chen ◽  
Liulian Huang
Keyword(s):  
Enzymatic Hydrolysis ◽  
High Ph ◽  
Lignin Removal ◽  
Pretreated Bagasse ◽  
Hydrolysis Of

Microwave-assisted enzymatic hydrolysis of starch

2009 ◽  
Author(s):  
Marcin Lukasiewicz ◽  
Anna Osowiec ◽  
Magdalena Marciniak
Keyword(s):  
Enzymatic Hydrolysis ◽  
Microwave Assisted ◽  
Hydrolysis Of

Analyses of enzymatic hydrolysis of bagasse cane of sugar for obtention of ethanol employing a cocktail of natives commercials enzymes.  

2018 ◽  
Author(s):  
Ángel Batallas ◽  
Erenio González ◽  
Carmen Salvador ◽  
Jonathan Villavicencio ◽  
Humberto González Gavilánez ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Hydrolysis Of

Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

2019 ◽  
Vol 15 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Swapnil Gaikwad ◽  
Avinash P. Ingle ◽  
Silvio Silverio da Silva ◽  
Mahendra Rai
Keyword(s):  
Catalytic Activity ◽  
Enzymatic Hydrolysis ◽  
Immobilized Enzyme ◽  
Free Enzyme ◽  
Magnetic Nanomaterials ◽  
Sugar Production ◽  
Cellulase Enzyme ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

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