Synthesis of Fluorescent Carbon Dots as Selective and Sensitive Probes for Cupric Ions and Cell Imaging

A novel sensing system has been designed for the detection of cupric ions. It is based on the quenched fluorescence signal of carbon dots (CDs), which were carbonized from poly(vinylpyrrolidone) (PVP) and L-Cysteine (CYS). Cupric ions interact with the nitrogen and sulfur atoms on surface of the CDs to form an absorbed complex; this results in strong quenching of the fluorescence of the CDs via a fast metal-to-ligand binding affinity. The synthesized water-soluble CDs also exhibited a quantum yield of 7.6%, with favorable photoluminescent properties and good photostability. The fluorescence intensity of the CDs was very stable in high ionic strength (up to 1.0 M NaCl) and over a wide range of pH levels (2.0–12.0). This facile method can therefore develop a sensor that offers reliable, fast, and selective detection of cupric ions with a detection limit down to 0.15 μM and a linear range from 0.5 to 7.0 μM (R2 = 0.980). The CDs were used for cell imaging, observed that they were low toxicity to Tramp C1 cells and exhibited blue and green and red fluorescence under a fluorescence microscope. In summary, the CDs exhibited excellent fluorescence properties, and could be applied to the selective and sensitive detection of cupric ion and multicolor cell imaging.

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Green synthesis of fluorescent carbon dots from Hongcaitai for selective detection of hypochlorite and mercuric ions and cell imaging

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2018.02.141 ◽

2018 ◽

Vol 263 ◽

pp. 426-435 ◽

Cited By ~ 47

Author(s):

Lu-Shuang Li ◽

Xin-Yue Jiao ◽

Yu Zhang ◽

Cheng Cheng ◽

Kun Huang ◽

...

Keyword(s):

Green Synthesis ◽

Carbon Dots ◽

Cell Imaging ◽

Selective Detection ◽

Mercuric Ions ◽

Fluorescent Carbon Dots ◽

Fluorescent Carbon

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Facile synthesis of water-soluble and biocompatible fluorescent nitrogen-doped carbon dots for cell imaging

The Analyst ◽

10.1039/c3an02098c ◽

2014 ◽

Vol 139 (7) ◽

pp. 1692-1696 ◽

Cited By ~ 91

Author(s):

Weiping Wang ◽

Ya-Chun Lu ◽

Hong Huang ◽

Jiu-Ju Feng ◽

Jian-Rong Chen ◽

...

Keyword(s):

Carbon Dots ◽

Cell Imaging ◽

Water Soluble ◽

Soluble Nitrogen ◽

Facile Synthesis ◽

Nitrogen Doped ◽

Low Toxicity ◽

Good Biocompatibility ◽

Doped Carbon Dots ◽

Nitrogen Doped Carbon

A simple and green hydrothermal method was developed for preparation of water-soluble nitrogen-doped carbon dots (N-CDs) from streptomycin. The prepared N-CDs reveal low toxicity, high stability and good biocompatibility, which can be used as fluorescent probes for cell imaging.

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Fabrication of polyethyleneimine surface-functionalized fluorescent carbon dots and its applications towards highly sensitive and selective detection of glutathione in aqueous medium and in vitro cell imaging of HeLa cells

Journal of Materials Science Materials in Electronics ◽

10.1007/s10854-021-06808-3 ◽

2021 ◽

Vol 32 (18) ◽

pp. 23215-23231

Author(s):

Zamir G. Khan ◽

Pravin O. Patil

Keyword(s):

Aqueous Medium ◽

Hela Cells ◽

Carbon Dots ◽

Cell Imaging ◽

Selective Detection ◽

Highly Sensitive ◽

Fluorescent Carbon Dots ◽

Fluorescent Carbon

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Water-soluble, nitrogen-doped fluorescent carbon dots for highly sensitive and selective detection of Hg2+ in aqueous solution

RSC Advances ◽

10.1039/c5ra04653j ◽

2015 ◽

Vol 5 (50) ◽

pp. 40393-40401 ◽

Cited By ~ 79

Author(s):

Y. Zhang ◽

Y. H. He ◽

P. P. Cui ◽

X. T. Feng ◽

L. Chen ◽

...

Keyword(s):

Carbon Dots ◽

Water Soluble ◽

Soluble Nitrogen ◽

Selective Detection ◽

One Pot ◽

Nitrogen Doped ◽

Highly Sensitive ◽

Doped Carbon Dots ◽

Nitrogen Doped Carbon ◽

Fluorescent Carbon Dots

Highly fluorescent water-soluble and nitrogen-doped carbon dots (NCDs) were synthesized by a simple one-pot hydrothermal method using citric acid as carbon source and urea as nitrogen source.

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Carbon Dots for Bacterial Detection and Antibacterial Applications-A Minireview

Current Pharmaceutical Design ◽

10.2174/1381612825666191216150948 ◽

2020 ◽

Vol 25 (46) ◽

pp. 4848-4860 ◽

Cited By ~ 2

Author(s):

Anisha Anand ◽

Gopinathan Manavalan ◽

Ranju Prasad Mandal ◽

Huan-Tsung Chang ◽

Yi-Ru Chiou ◽

...

Keyword(s):

Optical Properties ◽

Carbon Dots ◽

Antibacterial Effect ◽

Bacterial Detection ◽

High Brightness ◽

Photo Catalysis ◽

Wide Range ◽

Prevention And Treatment ◽

Low Toxicity ◽

Conventional Antibiotics

: The prevention and treatment of various infections caused by microbes through antibiotics are becoming less effective due to antimicrobial resistance. Researches are focused on antimicrobial nanomaterials to inhibit bacterial growth and destroy the cells, to replace conventional antibiotics. Recently, carbon dots (C-Dots) become attractive candidates for a wide range of applications, including the detection and treatment of pathogens. In addition to low toxicity, ease of synthesis and functionalization, and high biocompatibility, C-Dots show excellent optical properties such as multi-emission, high brightness, and photostability. C-Dots have shown great potential in various fields, such as biosensing, nanomedicine, photo-catalysis, and bioimaging. This review focuses on the origin and synthesis of various C-Dots with special emphasis on bacterial detection, the antibacterial effect of CDots, and their mechanism.

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Nitrogen and Sulfur Co-doped Fluorescent Carbon Dots for the Detection of Morin and Cell Imaging

Current Analytical Chemistry ◽

10.2174/1573411014666180904104629 ◽

2018 ◽

Vol 15 (1) ◽

pp. 47-55

Author(s):

Xuebing Li ◽

Haifen Yang ◽

Ning Wang ◽

Tijian Sun ◽

Wei Bian ◽

...

Keyword(s):

Microwave Heating ◽

Fluorescent Probe ◽

Fluorescence Intensity ◽

Carbon Dots ◽

Cell Imaging ◽

Water Soluble ◽

Heating Process ◽

X Ray ◽

Doped Carbon Dots ◽

Co Doped

Background: Morin has many pharmacological functions including antioxidant, anticancer, anti-inflammatory, and antibacterial effects. It is commonly used in the treatment of antiviral infection, gastropathy, coronary heart disease and hepatitis B in clinic. However, researches have shown that morin is likely to show prooxidative effects on the cells when the amount of treatment is at high dose, leading to the decrease of intracellular ATP levels and the increase of necrosis process. Therefore, it is necessary to determine the concentration of morin in biologic samples. Method: Novel water-soluble and green nitrogen and sulfur co-doped carbon dots (NSCDs) were prepared by a microwave heating process with citric acid and L-cysteine. The fluorescence spectra were collected at an excitation wavelength of 350 nm when solutions of NSCDs were mixed with various concentrations of morin. Results: The as-prepared NSCDs were characterized by transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The fluorescence intensity of NSCDs decreased significantly with the increase of morin concentration. The fluorescence intensity of NSCDs displayed a linear response to morin in the concentration 0.10-30 μM with a low detection limit of 56 nM. The proposed fluorescent probe was applied to analysis of morin in human body fluids with recoveries of 98.0-102%. Conclusion: NSCDs were prepared by a microwave heating process. The present analytical method is sensitive to morin. The quenching process between NSCDs and morin is attributed to the static quenching. In addition, the cellular toxicity on HeLa cells indicated that the as-prepared NSCDs fluorescent probe does not show obvious cytotoxicity in cell imaging. Our proposed method possibly opens up a rapid and nontoxic way for preparing heteroatom doped carbon dots with a broad application prospect.

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Yttrium‐mediated red fluorescent carbon dots for sensitive and selective detection of calcium ions

Luminescence ◽

10.1002/bio.4132 ◽

2021 ◽

Author(s):

Yan Zhang ◽

Li Li ◽

Juan Yue ◽

Lei Cao ◽

Pai Liu ◽

...

Keyword(s):

Calcium Ions ◽

Carbon Dots ◽

Selective Detection ◽

Fluorescent Carbon Dots ◽

Fluorescent Carbon

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Synthesis of Fluorescent Carbon Dots and Their Application in Ascorbic Acid Detection

Molecules ◽

10.3390/molecules26051246 ◽

2021 ◽

Vol 26 (5) ◽

pp. 1246

Author(s):

Tengfei Wang ◽

Hui Luo ◽

Xu Jing ◽

Jiali Yang ◽

Meijun Huo ◽

...

Keyword(s):

Ascorbic Acid ◽

Fluorescence Intensity ◽

Carbon Dots ◽

Ph Dependence ◽

Scale Up ◽

Water Soluble ◽

Jujube Fruit ◽

Fluorescent Carbon Dots ◽

Fluorescent Carbon

Water-soluble fluorescent carbon dots (CDs) were synthesized by a hydrothermal method using citric acid as the carbon source and ethylenediamine as the nitrogen source. The repeated and scale-up synthetic experiments were carried out to explore the feasibility of macroscopic preparation of CDs. The CDs/Fe3+ composite was prepared by the interaction of the CDs solution and Fe3+ solution. The optical properties, pH dependence and stability behavior of CDs or the CDs/Fe3+ composite were studied by ultraviolet spectroscopy and fluorescence spectroscopy. Following the principles of fluorescence quenching after the addition of Fe3+ and then the fluorescence recovery after the addition of asorbic acid, the fluorescence intensity of the carbon dots was measured at λex = 360 nm, λem = 460 nm. The content of ascorbic acid was calculated by quantitative analysis of the changing fluorescence intensity. The CDs/Fe3+ composite was applied to the determination of different active molecules, and it was found that the composite had specific recognition of ascorbic acid and showed an excellent linear relationship in 5.0–350.0 μmol·L−1. Moreover, the detection limit was 3.11 μmol·L−1. Satisfactory results were achieved when the method was applied to the ascorbic acid determination in jujube fruit. The fluorescent carbon dots composites prepared in this study may have broad application prospects in a rapid, sensitive and trace determination of ascorbic acid content during food processing.

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