scholarly journals Potent Vasodilator and Cellular Antioxidant Activity of Endemic Patagonian Calafate Berries (Berberis microphylla) with Nutraceutical Potential

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2700 ◽  
Author(s):  
Camila Calfío ◽  
Juan Pablo Huidobro-Toro
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Endothelial Cells ◽  
Liquid Chromatography ◽  
No Production ◽  
Liquid Chromatography Mass Spectrometry ◽  
Dpph Assay ◽  
Vascular Responses ◽  
Antioxidant Mechanism ◽  
Potent Vasodilator

Hydroalcoholic extracts of Patagonian Calafate berry (Berberis microphylla) contain mono or disaccharide conjugated anthocyanins and flavonols. The Liquid Chromatography-Mass Spectrometry (LC-MS) chemical extract profile identified glycosylated anthocyanidins such as delphinidin-, petunidin- and malvidin-3-glucoside as the major constituents. The predominant flavonols were 3-O substituents quercetin-rutinoside or -rhamnoside. Anthocyanins doubled flavonols in mass (13.1 vs. 6 mg/g extract). Polyphenols vascular actions were examined in the rat arterial mesenteric bed bioassay; extract perfusion elicited concentration-dependent vasodilatation mimicked by conjugated anthocyanins standards. Vascular responses of main glycosylated anthocyanins were endothelium-dependent (p < 0.001) and mediated by NO production (p < 0.05). The anthocyanins antioxidant activity determined in isolated endothelial cells (CAA) showed a reduced redox potential as compared to the extract or quercetin. While in the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, the anthocyanins showed an equivalent quercetin potency, the extract was 15-fold less active, proposing that the anthocyanin-induced vasodilation is not due to an antioxidant mechanism. The extract shows promising commercial nutraceutical potential.

scholarly journals COMPOSITION OF PURIFIED ANTHOCYANIN ISOLATED FROM TEAK AND IT’S IN VITRO ANTIOXIDANT ACTIVITY

2017 ◽  
Vol 9 (9) ◽  
pp. 258 ◽  
Author(s):  
Greeshma Murukan ◽  
Murugan K.
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
Oxygen Radical ◽  
Sulphonic Acid ◽  
Oxygen Radical Absorbance Capacity ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Objective: The present study evaluates purification, characterization of anthocyanin from in vitro culture of teak and its antioxidant potential.Methods: Anthocyanin was extracted from in vitro culture, purified by using amber lite XAD column and fractionated by Liquid chromatography mass spectrometry (LC-MS/MS). Various antioxidant assays were carried such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), Oxygen radical absorbance capacity (ORAC), Nitric oxide (NO) and Hydrogen peroxide (H2O2).Results: Liquid chromatography mass spectrometry (LC-MS/MS) revealed the major fraction as cyanidin 3-(2-xylosyl-rutinoside) with unknown peaks. The amount of anthocyanin was 15.23 mg/g monomeric anthocyanin. Further, the potential antioxidant capacity of the teak anthocyanin was comparable to common vegetables and fruits. Similarly, high correlations of anthocyanin with antioxidant activity, such as oxygen radical absorbance capacity (ORAC), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) (r = 0.95, 0.93, and 0.80) were found.Conclusion: The high anthocyanins content and potential antioxidant activity suggests that teak anthocyanin may be applied in the food industry as a good source of natural pigments

Rosa rubiginosa and Fraxinus oxycarpa herbal teas: characterization of phytochemical profiles by liquid chromatography-mass spectrometry, and evaluation of the antioxidant activity

2017 ◽  
Vol 41 (15) ◽  
pp. 7681-7688 ◽  
Author(s):  
J. Jiménez-López ◽  
A. Ruiz-Medina ◽  
P. Ortega-Barrales ◽  
E. J. Llorent-Martínez
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Folk Medicine ◽  
Liquid Chromatography Mass Spectrometry ◽  
Rosa Rubiginosa ◽  
Chromatography Mass Spectrometry ◽  
Phytochemical Profiles

The consumption of infusions and herbal teas has always been an important part of folk medicine.

scholarly journals AKTIVITAS ANTIOKSIDAN DAN IDENTIFIKASI SENYAWA EKSTRAK JAMUR LINGZHI (Ganoderma lucidum) DENGAN LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS)

EKOLOGIA ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 65-72
Author(s):  
Farida Nuraeni ◽  
Septi Bernadetha Br Sembiring
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Time Variation ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Dpph Method

Lingzhi mushroom is widely used as an alternative treatment to reduce blood pressure and blood sugar levels, to maximize the potential of the Lingzhi fungus antioxidant tests are carried out. This study aims to determine the potential antioxidant activity of water extract and 70% ethanol extract of Lingzhi mushroom with maceration extraction time variation and identification of compounds with Liquid Chromatography Mass Spectrometry (LC-MS). This research begins with the determination of fresh lingzhi mushrooms then made simplicia and extracted by maceration with time variation with soaking time of 24 hours, 48 hours and 72 hours each with 2 solvents namely water and 70% ethanol. The extract was tested by phytochemistry followed by testing the antioxidant activity of Lingzhi mushroom extract (ganoderma lucidum) by DPPH method. Then the compounds were identified by Liquid Chromatography-Mass Spectrometry (LC-MS). Based on the results of research that maceration for 1 hour (1 day) Lingzhi mushroom with 70% ethanol extraction has the potential as an antioxidant with an IC50 value of 94.83 ppm. The results of identification with LC-MS in 70% ethanol extract as a compound that has potential as an antioxidant are Bisphenol M compounds, and 1- - [[2- (3,4-Dimethoxyphenyl) amino]} -3-methyl-2-octylpyrido [1 , 2-a] benzimidazole-4-carbonitrile.

scholarly journals Liquid Chromatography Mass Spectrometry (LC-MS) Fingerprint Combined with Chemometrics for Identification of Metabolites Content and Biological Activities of Curcuma aeruginosa

2018 ◽  
Vol 18 (1) ◽  
pp. 43 ◽  
Author(s):  
Dewi Anggraini Septaningsih ◽  
Latifah Kosim Darusman ◽  
Farit Mochamad Afendi ◽  
Rudi Heryanto
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Herbal Medicine ◽  
Brine Shrimp ◽  
Biological Activities ◽  
Partial Least Square ◽  
Least Square ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chemometric Method

Curcuma aeruginosa is known as one of the components of herbal medicine with various biological activities. This research aims to identify the metabolites content of C. aeruginosa related to their biological activities using LC-MS fingerprint combined with chemometrics. C. aeruginosa from 3 areas in Java were collected and macerated with ethanol and then analyzed with LC-MS. Along with this analysis, the antioxidant activity of all samples was determined using CUPRAC method, and the toxicity was determined using Brine Shrimp Lethality Test (BSLT), and chemometric method was used Principle Component Analysis (PCA) and Partial Least square (PLS). Metabolites profiles showed 175 predicted compounds, in which the dominant compounds are from the sesquiterpene of Curcuma genus. The PCA metabolites profiles can separate the samples by their location of origin. Interpretation of the correlation between metabolites profiles and their bioactivities was determined using PLS technique. The results showed that the toxicity of samples was exerted by compounds with ion mass of 312.28 and 248.15, which have the highest antioxidant and toxicity potentials. Compounds with ion mass of 248.15 were predicted to be 9-Oxo-neoprocurcumenol, 7α,11α,-Epoxy-5β-hydroxy-9-guaiaen-8-one, Curcumenolactone A, or Curcumenolactone B. While compound with ion mass of 312.28 was predicted to tetrahydro-bisdemethoxycurcumin.

scholarly journals Strategy for Screening of Antioxidant Compounds from Two Ulmaceae Species Based on Liquid Chromatography-Mass Spectrometry

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1830 ◽  
Author(s):  
Joong Won ◽  
Su Son ◽  
Sunmin Lee ◽  
Digar Singh ◽  
Sarah Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Solid Phase ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Untargeted Metabolomics ◽  
Antioxidant Compounds ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics implies that annotated metabolites can serve as potential markers of the associated bioactivities of plant extracts. Firstly, we selected Aphananthe aspera and Zelkova serrata (Family: Ulmaceae) from 16 Korean plant species based on their distinct principal component analysis (PCA) patterns in LC-MS datasets and antioxidant activity assays. Further, we chose 40% solid-phase extraction (SPE) extracts of the two species displaying the highest antioxidant activities coupled with distinct PCA patterns. Examining the metabolite compositions of the 40% SPE extracts, we observed relatively higher abundances of quercetin, kaempferol, and isorhamnetin O-glucosides for A. aspera, whereas quercetin, isorhamnetin O-glucuronides, and procyanidin dimer were relatively higher in Z. serrata. These metabolites were clearly distinguished in pathway map and displayed strong positive correlations with antioxidant activity. Further, we performed preparative high-performance liquid chromatography (prep-HPLC) analysis coupled with the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assay to validate their functional correlations. As a result, quercetin O-sophoroside was determined as the main antioxidant in A. aspera, while isorhamnetin O-glucuronide and procyanidin dimer were the primary antioxidants in Z. serrata. The current study suggests that the LC-MS-based untargeted metabolomics strategy can be used to illuminate subtle metabolic disparities as well as compounds associated with bioactivities.

Sign in / Sign up

Export Citation Format

Share Document