Antibacterial Activity and Mode of Action of Dihydromyricetin from Ampelopsis grossedentata Leaves against Food-Borne Bacteria

Dihydromyricetin (DMY) has recently attracted increased interest due to its considerable health-promoting activities but there are few reports on its antibacterial activity and mechanism. In this paper, the activity and mechanisms of DMY from Ampelopsis grossedentata leaves against food-borne bacteria are investigated. Moreover, the effects of pH, thermal-processing, and metal ions on the antibacterial activity of DMY are also evaluated. The results show that DMY exhibits ideal antibacterial activity on five types of food-borne bacteria (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Salmonella paratyphi, and Pseudomonas aeruginosa). The activities of DMY against bacteria are extremely sensitive to pH, thermal-processing, and metal ions. The morphology of the tested bacteria is changed and damaged more seriously with the exposure time of DMY. Furthermore, the results of the oxidative respiratory metabolism assay and the integrity of the cell membrane and wall tests revealed that the death of bacteria caused by DMY might be due to lysis of the cell wall, leakage of intracellular ingredients, and inhibition of the tricarboxylic acid cycle (TCA) pathway.

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Effect of Penicillium frequentans and Stachybotrys chartarum on respiratory metabolism of developing eggs of Ascaris suum (Nematoda)

Acta Mycologica ◽

10.5586/am.2001.011 ◽

2014 ◽

Vol 36 (1) ◽

pp. 159-168

Author(s):

Lidia Kołodziejczyk ◽

Wanda Kuźna-Grygiel ◽

Krystyna Janowicz ◽

Kinga Mazurkiewicz-Zapałowicz

Keyword(s):

Lactate Dehydrogenase ◽

Soil Fungi ◽

Tricarboxylic Acid Cycle ◽

Ascaris Suum ◽

Tricarboxylic Acid ◽

Stachybotrys Chartarum ◽

Respiratory Metabolism ◽

Acid Cycle ◽

Penicillium Frequentans ◽

Cellular Oxidation

Effect of saprotrophic soil fungi Penicillium frequentans and Stachybotrys chartarum on respiratory metabolism of Ascaris suum, during its embryogenesis was determined using histoenzymatic methods. Based on histochemical assessment of the enzyme actvity (glycolysis-lactate dehydrogenase-LDH; tricarboxylic acid cycle-succinate dehydrogenase-SDH) changes in the energy metabolism of developing eggs of A. suvm were detected. Of the fungi species tested - P. frequentans caused the most extensive disorders in the processes of cellular oxidation, which were manifested in a decline of SDH activity during gastrulation. Incubation of eggs of Ascaris with mycelia of both fungi: S. chartarum and P. frequentans - in lesser extend affected respiratory metabolism in embryogenesis of this nematode.

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Analysis of tricarboxylic acid cycle intermediates in dried blood spots by ultra-performance liquid chromatography-tandem mass spectrometry

Journal of Biochemical and Clinical Genetics ◽

10.24911/jbcgenetics/183-1563341940 ◽

2019 ◽

pp. 1

Author(s):

Lamia Dirbashi

Keyword(s):

Mass Spectrometry ◽

Tricarboxylic Acid Cycle ◽

Dried Blood Spots ◽

Ultra Performance Liquid Chromatography ◽

Tricarboxylic Acid ◽

Tandem Mass ◽

Acid Cycle ◽

Chromatography Tandem Mass Spectrometry ◽

Liquid Chromatography Tandem Mass ◽

Tricarboxylic Acid Cycle Intermediates

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Faculty Opinions recommendation of Crosstalk between the tricarboxylic acid cycle and peptidoglycan synthesis in Caulobacter crescentus through the homeostatic control of α-ketoglutarate.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.729075299.793537174 ◽

2017 ◽

Author(s):

Kevin D Young ◽

Matthew Jorgenson

Keyword(s):

Caulobacter Crescentus ◽

Tricarboxylic Acid Cycle ◽

Tricarboxylic Acid ◽

Homeostatic Control ◽

Acid Cycle ◽

Peptidoglycan Synthesis

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Efficient Preconcentration of Cu2+, Zn2+ and Fe3+ with an Agarose-Schiff Base Sorbent

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc20070908 ◽

2007 ◽

Vol 72 (7) ◽

pp. 908-916 ◽

Cited By ~ 3

Author(s):

Payman Hashemi ◽

Hatam Hassanvand ◽

Hossain Naeimi

Keyword(s):

Schiff Base ◽

Metal Ions ◽

Metal Ion ◽

Good Accuracy ◽

Kinetic Studies ◽

Sample Volume ◽

Effects Of Ph ◽

Glass Column ◽

High Concentrations ◽

Ph Range

Sorption and preconcentration of Cu2+, Zn2+ and Fe3+ on a salen-type Schiff base, 2,2'- [ethane-1,2-diylbis(nitrilomethylidyne)]bis(2-methylphenol), chemically immobilized on a highly crosslinked agarose support, were studied. Kinetic studies showed higher sorption rates of Cu2+ and Fe3+ in comparison with Zn2+. Half-times (t1/2) of 31, 106 and 58 s were obtained for sorption of Cu2+, Zn2+ and Fe3+ by the sorbent, respectively. Effects of pH, eluent concentration and volume, ionic strength, buffer concentration, sample volume and interferences on the recovery of the metal ions were investigated. A 5-ml portion of 0.4 M HCl solution was sufficient for quantitative elution of the metal ions from 0.5 ml of the sorbent packed in a 6.5 mm i.d. glass column. Quantitative recoveries were obtained in a pH range 5.5-6.5 for all the analytes. The volumes to be concentrated exceeding 500 ml, ionic strengths as high as 0.5 mol l-1, and acetate buffer concentrations up to 0.3 mol l-1 for Zn2+ and 0.4 mol l-1 for Cu2+ and Fe3+ did not have any significant effect on the recoveries. The system tolerated relatively high concentrations of diverse ions. Preconcentration factors up to 100 and detection limits of 0.31, 0.16 and 1.73 μg l-1 were obtained for Cu2+, Zn2+ and Fe3+, respectively, for their determination by a flame AAS instrument. The method was successfully applied to the metal ion determinations in several river water samples with good accuracy.

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Modulation of tricarboxylic acid cycle dehydrogenases during hepatocarcinogenesis induced by hexachlorocyclohexane in mice

Experimental and Toxicologic Pathology ◽

10.1016/j.etp.2008.09.004 ◽

2009 ◽

Vol 61 (4) ◽

pp. 325-332 ◽

Cited By ~ 9

Author(s):

Devendra Kumar Bhatt ◽

Mehajbeen Bano

Keyword(s):

Tricarboxylic Acid Cycle ◽

Tricarboxylic Acid ◽

Acid Cycle

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Effects of sevoflurane anesthesia and abdominal surgery on the systemic metabolome: a prospective observational study

BMC Anesthesiology ◽

10.1186/s12871-021-01301-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Yiyong Wei ◽

Donghang Zhang ◽

Jin Liu ◽

Mengchan Ou ◽

Peng Liang ◽

...

Keyword(s):

Abdominal Surgery ◽

General Anesthesia ◽

Tricarboxylic Acid Cycle ◽

Tricarboxylic Acid ◽

Metabolic Changes ◽

Sevoflurane Anesthesia ◽

Glutamate Metabolism ◽

Acid Cycle ◽

Link Type ◽

The Mean

Abstract Background Metabolic status can be impacted by general anesthesia and surgery. However, the exact effects of general anesthesia and surgery on systemic metabolome remain unclear, which might contribute to postoperative outcomes. Methods Five hundred patients who underwent abdominal surgery were included. General anesthesia was mainly maintained with sevoflurane. The end-tidal sevoflurane concentration (ETsevo) was adjusted to maintain BIS (Bispectral index) value between 40 and 60. The mean ETsevo from 20 min after endotracheal intubation to 2 h after the beginning of surgery was calculated for each patient. The patients were further divided into low ETsevo group (mean − SD) and high ETsevo group (mean + SD) to investigate the possible metabolic changes relevant to the amount of sevoflurane exposure. Results The mean ETsevo of the 500 patients was 1.60% ± 0.34%. Patients with low ETsevo (n = 55) and high ETsevo (n = 59) were selected for metabolomic analysis (1.06% ± 0.13% vs. 2.17% ± 0.16%, P < 0.001). Sevoflurane and abdominal surgery disturbed the tricarboxylic acid cycle as identified by increased citrate and cis-aconitate levels and impacted glycometabolism as identified by increased sucrose and D-glucose levels in these 114 patients. Glutamate metabolism was also impacted by sevoflurane and abdominal surgery in all the patients. In the patients with high ETsevo, levels of L-glutamine, pyroglutamic acid, sphinganine and L-selenocysteine after sevoflurane anesthesia and abdominal surgery were significantly higher than those of the patients with low ETsevo, suggesting that these metabolic changes might be relevant to the amount of sevoflurane exposure. Conclusions Sevoflurane anesthesia and abdominal surgery can impact principal metabolic pathways in clinical patients including tricarboxylic acid cycle, glycometabolism and glutamate metabolism. This study may provide a resource data for future studies about metabolism relevant to general anaesthesia and surgeries. Trial registration www.chictr.org.cn. identifier: ChiCTR1800014327.

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