scholarly journals High-Throughput 1H-Nuclear Magnetic Resonance-Based Screening for the Identification and Quantification of Heartwood Diterpenic Acids in Four Black Pine (Pinus nigra Arn.) Marginal Provenances in Greece

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3603 ◽  
Author(s):  
Kostas Ioannidis ◽  
Eleni Melliou ◽  
Prokopios Magiatis
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Throughput ◽  
Resin Acid ◽  
Seed Orchard ◽  
Pinus Nigra ◽  
Resin Acids ◽  
Black Pine ◽  
Bioactive Substances ◽  
Nuclear Magnetic

A high-throughput quantitative Nuclear Magnetic Resonance 1H-NMR method was developed and applied to screen the quantity of the diterpenic resin acids in the heartwood of black pine, due to the renewed scientific interest in their medicinal properties and use in various diseases treatment. The 260 samples were taken from Pinus nigra clones, selected from four provenances of the Peloponnese (Greece), participating in a 35-year-old clonal seed orchard. Total resin acids per dry heartwood weight (dhw) varied greatly, ranging from 30.05 to 424.70 mg/gdhw (average 219.98 mg/gdhw). Abietic was the predominant acid (76.77 mg/gdhw), followed by palustric acid (47.94 mg/gdhw), neoabietic acid (39.34 mg/gdhw), and pimaric acid (22.54 mg/gdhw). Dehydroabietic acid was at moderate levels (11.69 mg/gdhw), while levopimaric, isopimaric, and sandaracopimaric acids were in lower concentrations. The resin acid fraction accounted for 72.33% of the total acetone extractives. Stilbenes were presented in significant quantities (19.70%). The resin acid content was composed mainly of the abietane type resin acids (83.56%). Peloponnesian Pinus nigra heartwood was found to be the richest source of resin acids identified to date and is considered the best natural source for the production of such bioactive extracts. The results indicate a high potential for effective selection and advanced breeding of pharmaceutical and high economic value bioactive substances from Pinus nigra clones.

scholarly journals Obtaining salts of resin acids from Cuban pine by metathesis reactions

2020 ◽  
Vol 10 (3) ◽  
pp. 5412-5417
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Resin Acid ◽  
Resin Acids ◽  
Added Value ◽  
Nuclear Magnetic Resonance Analysis ◽  
Metathesis Reactions ◽  
Biological Tests ◽  
Electron Dispersion ◽  
Nuclear Magnetic

The resin acids obtained from the Cuban pine rosin are the starting material for the development and application of metathesis reactions. These reactions allow the obtaining of salts with high added value which could be used in the development of biomaterials for dental use. The objective of this work was to obtain calcium, magnesium and zinc resinates from the resin acid obtained from the Cuban pine resin by metathesis reaction for possible use in the development of biomaterials. The products obtained were evaluated by elemental analysis, X-ray powder diffraction, infrared spectroscopy, scanning electron microscopy associated with electron dispersion spectroscopy, nuclear magnetic resonance and biological tests (antibacterial activity). The results showed the formation of the different resinates, observing the presence of cations in the salts obtained. The disappearance of the signal corresponding to the hydrogen of the carboxyl group was verified by nuclear magnetic resonance analysis due to the reaction between the resinic acid and the different metals studied. The biological analyzes showed that the best results are achieved with zinc resinate.

scholarly journals The extended lipid panel assay: a clinically-deployed high-throughput nuclear magnetic resonance method for the simultaneous measurement of lipids and Apolipoprotein B

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Erwin Garcia ◽  
Dennis W. Bennett ◽  
Margery A. Connelly ◽  
Elias J. Jeyarajah ◽  
Franklin C. Warf ◽  
...  
Keyword(s):  
Decision Making ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Throughput ◽  
Apolipoprotein B ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Lipid Panel ◽  
Compositional Diversity ◽  
Nuclear Magnetic

Abstract Background Standard lipid panel assays employing chemical/enzymatic methods measure total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C), from which are calculated estimates of low-density lipoprotein cholesterol (LDL-C). These lipid measures are used universally to guide management of atherosclerotic cardiovascular disease risk. Apolipoprotein B (apoB) is generally acknowledged to be superior to LDL-C for lipid-lowering therapeutic decision-making, but apoB immunoassays are performed relatively infrequently due to the added analytic cost. The aim of this study was to develop and validate the performance of a rapid, high-throughput, reagent-less assay producing an “Extended Lipid Panel” (ELP) that includes apoB, using the Vantera® nuclear magnetic resonance (NMR) analyzer platform already deployed clinically for lipoprotein particle and other testing. Methods Partial least squares regression models, using as input a defined region of proton NMR spectra of plasma or serum, were created to simultaneously quantify TC, TG, HDL-C, and apoB. Large training sets (n > ~ 1000) of patient sera analyzed independently for lipids and apoB by chemical methods were employed to ensure prediction models reflect the wide lipid compositional diversity of the population. The analytical performance of the NMR ELP assay was comprehensively evaluated. Results Excellent agreement was demonstrated between chemically-measured and ELP assay values of TC, TG, HDL-C and apoB with correlation coefficients ranging from 0.980 to 0.997. Within-run precision studies measured using low, medium, and high level serum pools gave coefficients of variation for the 4 analytes ranging from 1.0 to 3.8% for the low, 1.0 to 1.7% for the medium, and 0.9 to 1.3% for the high pools. Corresponding values for within-lab precision over 20 days were 1.4 to 3.6%, 1.2 to 2.3%, and 1.0 to 1.9%, respectively. Independent testing at three sites over 5 days produced highly consistent assay results. No major interference was observed from 38 endogenous or exogenous substances tested. Conclusions Extensive assay performance evaluations validate that the NMR ELP assay is efficient, robust, and substantially equivalent to standard chemistry assays for the clinical measurement of lipids and apoB. Routine reporting of apoB alongside standard lipid measures could facilitate more widespread utilization of apoB for clinical decision-making.

High-throughput determination of oil content in corn kernels using nuclear magnetic resonance imaging

2005 ◽  
Vol 82 (12) ◽  
pp. 855-862 ◽  
Author(s):  
John J. Kotyk ◽  
Marty D. Pagel ◽  
Kevin L. Deppermann ◽  
Ronald F. Colletti ◽  
Norman G. Hoffman ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Throughput ◽  
Oil Content ◽  
Nuclear Magnetic Resonance Imaging ◽  
Resonance Imaging ◽  
Corn Kernels ◽  
Nuclear Magnetic

scholarly journals The Extended Lipid Panel Assay: A Clinically-Deployed High-Throughput Nuclear Magnetic Resonance Method for the Simultaneous Measurement of Lipids and Apolipoprotein B

2020 ◽  
Author(s):  
Erwin Garcia ◽  
Dennis W. Bennett ◽  
Margery A. Connelly ◽  
Elias J. Jeyarajah ◽  
Franklin C. Warf ◽  
...  
Keyword(s):  
Decision Making ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Throughput ◽  
Apolipoprotein B ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Lipid Panel ◽  
Compositional Diversity ◽  
Nuclear Magnetic

Abstract BackgroundStandard lipid panel assays employing chemical/enzymatic methods measure total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C), from which are calculated estimates of low-density lipoprotein cholesterol (LDL-C). These lipid measures are used universally to guide management of atherosclerotic cardiovascular disease risk. Apolipoprotein B (apoB) is generally acknowledged to be superior to LDL-C for lipid-lowering therapeutic decision-making, but apoB immunoassays are performed relatively infrequently due to the added analytic cost. The aim of this study was to develop and validate the performance of a rapid, high-throughput, reagent-less assay producing an “Extended Lipid Panel” (ELP) that includes apoB, using the Vantera® nuclear magnetic resonance (NMR) analyzer platform already deployed clinically for lipoprotein particle and other testing.MethodsPartial least squares regression models, using as input a defined region of proton NMR spectra of plasma or serum, were created to simultaneously quantify TC, TG, HDL-C, and apoB. Large training sets (n >~1,000) of patient sera analyzed independently for lipids and apoB by chemical methods were employed to ensure prediction models reflect the wide lipid compositional diversity of the population. The analytical performance of the NMR ELP assay was comprehensively evaluated.ResultsExcellent agreement was demonstrated between chemically-measured and ELP assay values of TC, TG, HDL-C and apoB with correlation coefficients ranging from 0.980 to 0.997. Within-run precision studies measured using low, medium, and high level serum pools gave coefficients of variation for the 4 analytes ranging from 1.0 to 3.8% for the low, 1.0 to 1.7% for the medium, and 0.9 to 1.3% for the high pools. Corresponding values for within-lab precision over 20 days were 1.4 to 3.6%, 1.2 to 2.3%, and 1.0 to 1.9%, respectively. Independent testing at three sites over 5 days produced highly consistent assay results. No major interference was observed from 38 endogenous or exogenous substances tested.ConclusionsExtensive assay performance evaluations validate that the NMR ELP assay is efficient, robust, and substantially equivalent to standard chemistry assays for the clinical measurement of lipids and apoB. Routine reporting of apoB alongside standard lipid measures could facilitate more widespread utilization of apoB for clinical decision-making.

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