Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

The solvent-assisted lipid bilayer (SALB) formation method provides a simple and efficient, microfluidic-based strategy to fabricate supported lipid bilayers (SLBs) with rich compositional diversity on a wide range of solid supports. While various studies have been performed to characterize SLBs formed using the SALB method, relatively limited work has been carried out to understand the underlying mechanisms of SALB formation under various experimental conditions. Through thermodynamic modeling, we studied the experimental parameters that affect the SALB formation process, including substrate surface properties, initial lipid concentration, and temperature. It was found that all the parameters are critically important to successfully form high-quality SLBs. The model also helps to identify the range of parameter space within which conformal, homogeneous SLBs can be fabricated, and provides mechanistic guidance to optimize experimental conditions for lipid membrane-related applications.

Insights into Magma Storage Beneath a Frequently Erupting Arc Volcano (Villarrica, Chile) from Unsupervised Machine Learning Analysis of Mineral Compositions

A key method to investigate magma dynamics is the analysis of the crystal cargoes carried by erupted magmas. These cargoes may comprise crystals that crystallize in different parts of the magmatic system (throughout the crust) and/or different times. While an individual eruption likely provides a partial view of the sub-volcanic plumbing system, compiling data from multiple eruptions builds a picture of the whole magmatic system. In this study we use machine learning techniques to analyze a large (>2000) compilation of mineral compositions from a highly active arc volcano: Villarrica, Chile. Villarrica's post-glacial eruptive activity (14 ka–present) displays large variation in eruptive style (mafic ignimbrites to Hawaiian effusive eruptions) yet its eruptive products have a near constant basalt-basaltic andesite bulk-rock composition. What, therefore, is driving explosive eruptions at Villarrica and can differences in storage dynamics be related to eruptive style? We used hierarchical cluster analysis to detect previously undetected structure in olivine, plagioclase and clinopyroxene compositions, revealing the presence of compositionally distinct clusters. Using rhyolite-MELTS thermodynamic modeling we related these clusters to intensive magmatic variables: temperature, pressure, water content and oxygen fugacity. Our results provide evidence for the existence of multiple discrete (spatial and temporal) magma reservoirs beneath Villarrica where melts differentiate and mix with incoming more primitive magma. The compositional diversity of an erupted crystal cargo strongly correlates with eruptive intensity, and we postulate that mixing between primitive and differentiated magma drives explosive activity at Villarrica.

New Perspectives on the Exoplanet Radius Gap from a Mathematica Tool and Visualized Water Equation of State

Recent astronomical observations obtained with the Kepler and TESS missions and their related ground-based follow-ups revealed an abundance of exoplanets with a size intermediate between Earth and Neptune (1 R ⊕ ≤ R ≤ 4 R ⊕). A low occurrence rate of planets has been identified at around twice the size of Earth (2 × R ⊕), known as the exoplanet radius gap or radius valley. We explore the geometry of this gap in the mass–radius diagram, with the help of a Mathematica plotting tool developed with the capability of manipulating exoplanet data in multidimensional parameter space, and with the help of visualized water equations of state in the temperature–density (T–ρ) graph and the entropy–pressure (s–P) graph. We show that the radius valley can be explained by a compositional difference between smaller, predominantly rocky planets (<2 × R ⊕) and larger planets (>2 × R ⊕) that exhibit greater compositional diversity including cosmic ices (water, ammonia, methane, etc.) and gaseous envelopes. In particular, among the larger planets (>2 × R ⊕), when viewed from the perspective of planet equilibrium temperature (T eq), the hot ones (T eq ≳ 900 K) are consistent with ice-dominated composition without significant gaseous envelopes, while the cold ones (T eq ≲ 900 K) have more diverse compositions, including various amounts of gaseous envelopes.

Geology and Petrology of Ruapehu Volcano and Related Vents

<p>Ruapehu Volcano is an active, multiple-vent, andesite composite volcano at the southern terminus of the Taupo Volcanic Zone, central North Island, New Zealand. The present-day volume of Ruapehu is estimated at 110 km3, and construction of the massif probably occurred during the past 0.5 m.y. Geologic mapping and stratigraphic studies have led to the recognition of four periods of cone construction, each occurring over 104-105 year time intervals. On the basis of lithologic/petrographic differences, and conspicuous unconformities which separate the deposits of each cone-building period, four new formations are defined, comprises the Ruapehu Group. Te Herenga formation (new formation name) comprises the oldest deposits of Ruapehu (upper lavas ca. 0.23 Ma) and is exposed as planeze surfaces and aretes on N and NW Ruapehu. The formation includes lava flows, tuff breccias, and small intrusive bodies surrounded by zones of hydrothermal alteration. There is little petrographic and compositional diversity; most lavas are porphyritic titanomagnetite- augite- hypersthene- plagioclase basic andesites. Wahiance Formation (new formation name) is younger than Te Herenga Fm,. but of unknown age. It is well exposed on SE Ruapehu, and comprises mostly lava flows and tuff breccias. The lavas comprise acid and basic andesites. Mangewhero Formation (new formation name) is well exposed everywhere except SE Ruapehu, and the upper lavas and pyroclastics (ca. 0.02 Ma) form the present high peeks and main cone of Ruapehu. The lavas are petrographically and geochemically diverse, ranging from basalt to decite in bulk composition. Some of the lower lavas are olivine-beering andesites of hybrid orgin. Whakapapa Formation (new formation name; ca 15,000 years to present) comprises conspicuously young lava flows, tuff breccias, airfall pyroclastics and minor pyroclastic flows of acid- and basic andesite. The deposits of these post-glacial summit and flank eruptions are subdivided into the lwikau, Rangataua, Tama and Crater Lake Members. 'Related vents' produced Heuhungatahi Andesite Fm. (> 0.5 Ma?), and Holocene deposits of basalt and basic andesite at isolated, monogenetic centres comprising Ohakune Andesite Fm., Pukeonake Andesite Fm., and Waimarino Basalt Fm. (new formation name). Most Ruapehu lavas are medium-K acid and basic andesites (mean of 144 bulk rock analyses is 57.8 wt % SiO2), but rare basalt and minor decite are present. Nearly all lavas are porphyritic in plagioclase, augite and hypersthene [plus or minus] olivine, with titanomagnetite micro- phenocrysts, and contain abundant metamorphic and igneous rock inclusions. Petrography, mineral chemistry and bulk rock chemistry indicate fractional crystallization series from parental basalts (52-53 % SiO2, Q-normative, low-alumina) to medium-K basic- and acid andesites (58-59 % SiO2). Early fractionating minerals are olivine and clinopyroxene with minor chrome spinel and plagioclase, followed by plagioclase, orthopyroxene, clinopyroxene and minor titanomagnetite in later stages of differentiation. Thus, basalt differentiation to produce andesites involves 'POAM-type' (Gill, 1981) fractional crystallization. Three second-order differentiation processes operate concurrently with frational crystallization: (1) Crystal accumulation involves addition of co-genetic plutonic rock fragments and crystals derived from them. These inclusions are common and few rocks represent liquid compositions. (2) Magma mixing involves mingling of magmas in repeatedly-occupied conduits. End members are as diverse as basalt and decite, yielding petrogaphically and chemically distinctive high-Mg andesites of the upper cone complex and parasitic centres. (3) Selective crustal assimilation is suggested by partially fused metamorphic inclusions, positive correlation of 87Sr/86Sr with SiO2, and failure of simple 'POAM' fractionation to explain decites (63-65 % SiO2). Petrogenesis of Ruapehu andesites takes place under open-system condition, involving production of parental Q-normative basalts in the mantle wedge, concurrent fractional crystallization and crustal contamination, entrainment of co-genetic plutonic rocks, and mixing of magmas in common conduits.</p>

Geology and Petrology of Ruapehu Volcano and Related Vents

<p>Ruapehu Volcano is an active, multiple-vent, andesite composite volcano at the southern terminus of the Taupo Volcanic Zone, central North Island, New Zealand. The present-day volume of Ruapehu is estimated at 110 km3, and construction of the massif probably occurred during the past 0.5 m.y. Geologic mapping and stratigraphic studies have led to the recognition of four periods of cone construction, each occurring over 104-105 year time intervals. On the basis of lithologic/petrographic differences, and conspicuous unconformities which separate the deposits of each cone-building period, four new formations are defined, comprises the Ruapehu Group. Te Herenga formation (new formation name) comprises the oldest deposits of Ruapehu (upper lavas ca. 0.23 Ma) and is exposed as planeze surfaces and aretes on N and NW Ruapehu. The formation includes lava flows, tuff breccias, and small intrusive bodies surrounded by zones of hydrothermal alteration. There is little petrographic and compositional diversity; most lavas are porphyritic titanomagnetite- augite- hypersthene- plagioclase basic andesites. Wahiance Formation (new formation name) is younger than Te Herenga Fm,. but of unknown age. It is well exposed on SE Ruapehu, and comprises mostly lava flows and tuff breccias. The lavas comprise acid and basic andesites. Mangewhero Formation (new formation name) is well exposed everywhere except SE Ruapehu, and the upper lavas and pyroclastics (ca. 0.02 Ma) form the present high peeks and main cone of Ruapehu. The lavas are petrographically and geochemically diverse, ranging from basalt to decite in bulk composition. Some of the lower lavas are olivine-beering andesites of hybrid orgin. Whakapapa Formation (new formation name; ca 15,000 years to present) comprises conspicuously young lava flows, tuff breccias, airfall pyroclastics and minor pyroclastic flows of acid- and basic andesite. The deposits of these post-glacial summit and flank eruptions are subdivided into the lwikau, Rangataua, Tama and Crater Lake Members. 'Related vents' produced Heuhungatahi Andesite Fm. (> 0.5 Ma?), and Holocene deposits of basalt and basic andesite at isolated, monogenetic centres comprising Ohakune Andesite Fm., Pukeonake Andesite Fm., and Waimarino Basalt Fm. (new formation name). Most Ruapehu lavas are medium-K acid and basic andesites (mean of 144 bulk rock analyses is 57.8 wt % SiO2), but rare basalt and minor decite are present. Nearly all lavas are porphyritic in plagioclase, augite and hypersthene [plus or minus] olivine, with titanomagnetite micro- phenocrysts, and contain abundant metamorphic and igneous rock inclusions. Petrography, mineral chemistry and bulk rock chemistry indicate fractional crystallization series from parental basalts (52-53 % SiO2, Q-normative, low-alumina) to medium-K basic- and acid andesites (58-59 % SiO2). Early fractionating minerals are olivine and clinopyroxene with minor chrome spinel and plagioclase, followed by plagioclase, orthopyroxene, clinopyroxene and minor titanomagnetite in later stages of differentiation. Thus, basalt differentiation to produce andesites involves 'POAM-type' (Gill, 1981) fractional crystallization. Three second-order differentiation processes operate concurrently with frational crystallization: (1) Crystal accumulation involves addition of co-genetic plutonic rock fragments and crystals derived from them. These inclusions are common and few rocks represent liquid compositions. (2) Magma mixing involves mingling of magmas in repeatedly-occupied conduits. End members are as diverse as basalt and decite, yielding petrogaphically and chemically distinctive high-Mg andesites of the upper cone complex and parasitic centres. (3) Selective crustal assimilation is suggested by partially fused metamorphic inclusions, positive correlation of 87Sr/86Sr with SiO2, and failure of simple 'POAM' fractionation to explain decites (63-65 % SiO2). Petrogenesis of Ruapehu andesites takes place under open-system condition, involving production of parental Q-normative basalts in the mantle wedge, concurrent fractional crystallization and crustal contamination, entrainment of co-genetic plutonic rocks, and mixing of magmas in common conduits.</p>

Impact of antibiotics to off-target infant gut microbiota and resistance genes in cohort studies

Background: Young children are frequently exposed to antibiotics for otitis media and respiratory infections, with the potential for collateral consequences on the gut microbiome. The impact of antibiotic exposures to off-target microbes (i.e., bacteria not targeted by antibiotic treatment) and antibiotic resistance genes (ARGs) is unknown. Methods: We used metagenomic sequencing data from paired stool samples collected prior to antibiotic exposure and at 1 year from over 200 infants and a difference-in-differences approach to assess the relationship between subsequent exposures and the abundance or compositional diversity of off-target microbes and ARGs while adjusting for covariates. Results: By 1 year, the relative abundance of multiple species and ARGs differed by antibiotic exposure. Compared to infants never exposed to antibiotics, Bacteroides vulgatus relative abundance increased by 1.72% (95%CI:0.19,3.24) while Bacteroides fragilis decreased by 1.56% (95%CI:-4.32,1.21). Bifidobacterium species also exhibited opposing trends suggesting differential antibiotic selection. Overall, antibiotic exposure was associated with a dose-dependent decrease in alpha diversity of off-target microbes. ARGs associated with antibiotic exposure included class A beta-lactamase gene CfxA6. Among infants attending day care, Escherichia coli and ARG abundance were both positively associated with antibiotic use. Conclusion: Further quantifying impacts to off-target microbes and ARGs has implications for antibiotic stewardship

VLT/SPHERE imaging survey of the largest main-belt asteroids: Final results and synthesis

Context. Until recently, the 3D shape, and therefore density (when combining the volume estimate with available mass estimates), and surface topography of the vast majority of the largest (D ≥ 100 km) main-belt asteroids have remained poorly constrained. The improved capabilities of the SPHERE/ZIMPOL instrument have opened new doors into ground-based asteroid exploration. Aims. To constrain the formation and evolution of a representative sample of large asteroids, we conducted a high-angular-resolution imaging survey of 42 large main-belt asteroids with VLT/SPHERE/ZIMPOL. Our asteroid sample comprises 39 bodies with D ≥ 100 km and in particular most D ≥ 200 km main-belt asteroids (20/23). Furthermore, it nicely reflects the compositional diversity present in the main belt as the sampled bodies belong to the following taxonomic classes: A, B, C, Ch/Cgh, E/M/X, K, P/T, S, and V. Methods. The SPHERE/ZIMPOL images were first used to reconstruct the 3D shape of all targets with both the ADAM and MPCD reconstruction methods. We subsequently performed a detailed shape analysis and constrained the density of each target using available mass estimates including our own mass estimates in the case of multiple systems. Results. The analysis of the reconstructed shapes allowed us to identify two families of objects as a function of their diameters, namely “spherical” and “elongated” bodies. A difference in rotation period appears to be the main origin of this bimodality. In addition, all but one object (216 Kleopatra) are located along the Maclaurin sequence with large volatile-rich bodies being the closest to the latter. Our results further reveal that the primaries of most multiple systems possess a rotation period of shorter than 6 h and an elongated shape (c∕a ≤ 0.65). Densities in our sample range from ~1.3 g cm−3 (87 Sylvia) to ~4.3 g cm−3 (22 Kalliope). Furthermore, the density distribution appears to be strongly bimodal with volatile-poor (ρ ≥ 2.7 g cm−3) and volatile-rich (ρ ≤ 2.2 g cm−3) bodies. Finally, our survey along with previous observations provides evidence in support of the possibility that some C-complex bodies could be intrinsically related to IDP-like P- and D-type asteroids, representing different layers of a same body (C: core; P/D: outer shell). We therefore propose that P/ D-types and some C-types may have the same origin in the primordial trans-Neptunian disk.