scholarly journals The Trichoderma atroviride Strains P1 and IMI 206040 Differ in Their Light-Response and VOC Production

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 208 ◽  
Author(s):  
Verena Speckbacher ◽  
Veronika Ruzsanyi ◽  
Modestus Wigger ◽  
Susanne Zeilinger
Keyword(s):  
Organic Compounds ◽  
Plant Protection ◽  
Light Response ◽  
Systemic Resistance ◽  
Model Organisms ◽  
Trichoderma Atroviride ◽  
Dependent Behavior ◽  
Volatile Organic ◽  
Secondary Metabolite Biosynthesis ◽  
Fungal Phytopathogens

Trichoderma atroviride is a strong necrotrophic mycoparasite antagonizing and feeding on a broad range of fungal phytopathogens. It further beneficially acts on plants by enhancing growth in root and shoot and inducing systemic resistance. Volatile organic compounds (VOCs) are playing a major role in all those processes. Light is an important modulator of secondary metabolite biosynthesis, but its influence has often been neglected in research on fungal volatiles. To date, T. atroviride IMI 206040 and T. atroviride P1 are among the most frequently studied T. atroviride strains and hence are used as model organisms to study mycoparasitism and photoconidiation. However, there are no studies available, which systematically and comparatively analyzed putative differences between these strains regarding their light-dependent behavior and VOC biosynthesis. We therefore explored the influence of light on conidiation and the mycoparasitic interaction as well as the light-dependent production of VOCs in both strains. Our data show that in contrast to T. atroviride IMI 206040 conidiation in strain P1 is independent of light. Furthermore, significant strain- and light-dependent differences in the production of several VOCs between the two strains became evident, indicating that T. atroviride P1 could be a better candidate for plant protection than IMI 206040.

Biocidal effects of volatile organic compounds produced by the myxobacterium Corrallococcus sp. EGB against fungal phytopathogens

2020 ◽  
Vol 91 ◽  
pp. 103502
Author(s):  
Xianfeng Ye ◽  
Yang Chen ◽  
Shiyun Ma ◽  
Tian Yuan ◽  
Yaxuan Wu ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Volatile Organic ◽  
Fungal Phytopathogens

scholarly journals MASS SPECTROMETRIC STUDIES OF THE COMPOSITION OF VOLATILE ORGANIC COMPOUNDS RELEASED BY VARIOUS FUNGAL SPECIES OF GENUS LECANICILLIUM

2021 ◽  
Vol 31 (4) ◽  
pp. 71-78
Author(s):  
A. G. Kuzmin ◽  
Keyword(s):  
Acetic Acid ◽  
Volatile Organic Compounds ◽  
Gas Phase ◽  
Organic Compounds ◽  
Plant Protection ◽  
Fungal Species ◽  
Quadrupole Mass ◽  
Volatile Organic ◽  
First Time ◽  
Chemical Pesticides

For the first time, the quantitative and qualitative composition of volatile organic compounds (VOCs), released by strains of various species of entomopathogenic fungi (EF) of the genus Lecanicillium, was studied using a quadrupole mass spectrometer. Lecanicillium fungi are used as an alternative to chemical pesticides for plant protection. The main detected components of the gas phase over the EF mycelium on the 10th day of growing on the agar Czapek's medium were carbon dioxide (5–20%), oxygen (0.1–15%), acetone (0.2–12 ppm), pentane (up to 0.5 ppm), acetic acid (up to 0.15 ppm). Acetone and pentane were found in the VOCs of all studied strains, acetic acid — in 5 strains belonging to different species, in other strains it appeared after a longer period of time, or was absent completely. Among the VOCs of some strains, substances such as hexyl acetate, sulfur dioxide were found in small quantities. These substances may be responsible for the pathogenic and repellent properties of the studied fungi with respect to phytophages.

scholarly journals Volatile Organic Compounds of Endophytic Burkholderia pyrrocinia Strain JK-SH007 Promote Disease Resistance in Poplar

Plant Disease ◽  
2020 ◽  
Vol 104 (6) ◽  
pp. 1610-1620 ◽  
Author(s):  
Ake Liu ◽  
Pengfei Zhang ◽  
Bianxia Bai ◽  
Fenglin Bai ◽  
Tingting Jin ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Solid Phase ◽  
Systemic Resistance ◽  
Gas Chromatography Mass Spectrometry ◽  
Ethylene Response Factor ◽  
Defense Enzyme ◽  
Volatile Organic ◽  
Canker Pathogens ◽  
Poplar Seedlings

Volatile organic compounds (VOCs) play important roles in the regulation of plant growth and pathogen resistance. However, little is known about the influence of VOCs released from endophytic strains (Burkholderia pyrrocinia strain JK-SH007) on controlling pathogens or inducing systemic resistance in poplar. In this study, we found that VOCs produced by strain JK-SH007 inhibit three poplar canker pathogens (Cytospora chrysosperma, Phomopsis macrospora, and Fusicoccum aesculi) and promote defense enzyme activity and malondialdehyde (MDA) and total phenol (TP) accumulation. Thirteen kinds of VOC components were identified using the solid-phase microextraction combined with gas chromatography-mass spectrometry method. Dimethyl disulfide (DMDS) accounted for the largest proportion of these VOCs. Treatments of poplar seedlings with different volumes of VOC standards (DMDS, benzothiazole, dimethylthiomethane, and phenylacetone) showed that DMDS had the greatest effects on various defense enzyme activities and MDA and TP accumulation. We also found that the inhibitory effect of the VOCs on the three pathogens was gradually enhanced with increasing standard volume. Moreover, the treatment of samples with DMDS significantly reduced the severity and development of the disease caused by three poplar canker pathogens. Comparative transcriptomics analysis of poplar seedlings treated with DMDS showed that there were 1,586 differentially expressed genes in the leaves and stems, and quantitative PCR showed that the gene expression trends were highly consistent with the transcriptome sequencing results. The most significant transcriptomic changes induced by VOCs were related to hormone signal transduction, transcriptional regulation of plant–pathogen interactions, and energy metabolism. Moreover, 137 transcription factors, including members of the ethylene response factor, NAC, WRKY, G2-like, and basic helix-loop-helix protein families, were identified to be involved in the VOC-induced process. This study elucidates the resistance induced by Burkholderia pyrrocinia strain JK-SH007 to poplar canker at the molecular level and can make possible a new method for the comprehensive prevention and control of poplar disease.

Beetle Orientation Responses of Gastrophysa viridula and Gastrophysa polygoni (Coleoptera: Chrysomelidae) to a Blend of Synthetic Volatile Organic Compounds

2020 ◽  
Vol 49 (5) ◽  
pp. 1071-1076
Author(s):  
Dariusz Piesik ◽  
Jan Bocianowski ◽  
Sebastian Sendel ◽  
Krzysztof Krawczyk ◽  
Karol Kotwica
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Plant Protection ◽  
Leaf Beetle ◽  
Aromatic Plants ◽  
Invasive Weed ◽  
Leaf Beetles ◽  
Chemical Treatments ◽  
Plant Volatile ◽  
Volatile Organic

Abstract The invasive weed Rumex confertus Willd. (mossy sorrel) is eaten and severely defoliated by oligophagous Gastrophysa viridula Deg. (dock leaf beetle) and Gastrophysa polygoni L. (knotweed leaf beetle). The most popular methods of plant protection involve the application of chemicals, but such methods often require repeated chemical treatments. Aromatic plants may constitute an eco-friendly alternative strategy owing to their repellent properties. To date, single compounds have been tested rather than blends; however, there is a need to investigate mixtures of compounds, because insects are subjected to blends of odors derived from their surrounding environments. The aim of the current study was to investigate behavioral responses of the dock leaf beetle and knotweed leaf beetle to a blend of synthetic plant volatile organic compounds. Plants were treated with standard repellents (a blend of volatile organic compounds) at two different concentrations (10 ng min−1 and 1,000 ng min−1). For further experiments, four rates (1 ng min−1, 10 ng min−1, 100 ng min−1, and 1,000 ng min−1 in 50 µl) were evaluated using a 4-way olfactometer. Leaf beetles of both sexes were repelled by the highest three concentrations tested. Female dock leaf beetles were also repelled by the lowest concentration tested, where individual components could have occasionally attracted insects. These results indicate a difference in responses to individual compounds and mixtures of compounds.

scholarly journals Systemic Resistance Induced by Volatile Organic Compounds Emitted by Plant Growth-Promoting Fungi in Arabidopsis thaliana

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86882 ◽  
Author(s):  
Hushna Ara Naznin ◽  
Daigo Kiyohara ◽  
Minako Kimura ◽  
Mitsuo Miyazawa ◽  
Masafumi Shimizu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Volatile Organic Compounds ◽  
Plant Growth ◽  
Organic Compounds ◽  
Systemic Resistance ◽  
Volatile Organic ◽  
Plant Growth Promoting ◽  
Growth Promoting
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