scholarly journals Effect of Lignin Content on Cellulolytic Saccharification of Liquid Hot Water Pretreated Sugarcane Bagasse

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 623 ◽  
Author(s):  
Rafaela I. S. Ladeira Ázar ◽  
Sidnei Emilio Bordignon-Junior ◽  
Craig Laufer ◽  
Jordan Specht ◽  
Drew Ferrier ◽  
...  

Lignin contributes to the rigid structure of the plant cell wall and is partially responsible for the recalcitrance of lignocellulosic materials to enzymatic digestion. Overcoming this recalcitrance is one the most critical issues in a sugar-flat form process. This study addresses the effect of low lignin sugarcane bagasse on enzymatic hydrolysis after liquid hot water pretreatment at 190 °C and 20 min (severity factor: 3.95). The hydrolysis of bagasse from a sugarcane line selected for a relatively low lignin content, gave an 89.7% yield of cellulose conversion to glucose at 40 FPU/g glucan versus a 68.3% yield from a comparably treated bagasse from the high lignin bred line. A lower enzyme loading of 5 FPU/g glucan (equivalent to 3.2 FPU/g total solids) resulted in 31.4% and 21.9% conversion yields, respectively, for low and high lignin samples, suggesting the significance of lignin content in the saccharification process. Further increases in the enzymatic conversion of cellulose to glucose were achieved when the bagasse sample was pre-incubated with a lignin blocking agent, e.g., bovine serum albumin (50 mg BSA/g glucan) at 50 °C for 1 h prior to an actual saccharification. In this work, we have demonstrated that even relatively small differences in lignin content can result in considerably increased sugar production, which supports the dissimilarity of bagasse lignin content and its effects on cellulose digestibility. The increased glucose yields with the addition of BSA helped to decrease the inhibition of non-productive absorption of cellulose enzymes onto lignin and solid residual lignin fractions.

2014 ◽  
Vol 472 ◽  
pp. 774-779 ◽  
Author(s):  
Hong Dan Zhang ◽  
Shu Bin Wu

Liquid hot water pretreatment, as an initial step in an alternative use of lignocellulosic biomass to produce fermentable sugar, was performed in this study. The effect of pretreatment temperature range from 160 to 200 °C on the hemicellulose degradation (the yields of glucose and xylose, as well as inhibitors) and cellulose enzymatic digestibility were evaluated. The results indicated that the maximum xylose yields (combined 2.23 g xylose and 13.20 g xylo-oligosaccharides per 100g raw material) in prehydrolysate liquid were obtained at 180 °C. The untreated and pretreated solid residues were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FT-IR). The results showed that liquid hot water pretreatment removed a large number of hemicellulose and resulted in enriched cellulose and lignin content in the pretreated residues. Due to the effective removal of hemicellulose, the maximum glucose yield in enzyme hydrolyzate reached 37.27 g per 100 g raw material (after the pretreatment temperature of 200°C), representing 90.13% of glucose in the sugarcane bagasse.


BioResources ◽  
2014 ◽  
Vol 10 (1) ◽  
Author(s):  
Qiang Yu ◽  
Chunxun Xu ◽  
Xinshu Zhuang ◽  
Zhenhong Yuan ◽  
Minchao He ◽  
...  

2009 ◽  
Vol 25 (2) ◽  
pp. 340-348 ◽  
Author(s):  
Youngmi Kim ◽  
Nathan S. Mosier ◽  
Michael R. Ladisch

2011 ◽  
Vol 27 (2) ◽  
pp. 395-401 ◽  
Author(s):  
Fernanda M. Mendes ◽  
Germano Siqueira ◽  
Walter Carvalho ◽  
André Ferraz ◽  
Adriane M. F. Milagres

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 463
Author(s):  
Soo-Kyeong Jang ◽  
Jong-Hwa Kim ◽  
June-Ho Choi ◽  
Seong-Min Cho ◽  
Jong-Chan Kim ◽  
...  

Eucalyptus pellita is known as attractive biomass, and it has been utilized for eucalyptus oil, furniture, and pulp and paper production that causes a significant amount of byproducts. Liquid hot water treatment depending on combined severity factor (CSF) was subjected to isolate hemicellulose fraction from E. pellita and to produce xylooligosaccharides (XOS). The xylan extraction ratio based on the initial xylan content of the feedstock was maximized up to 77.6% at 170 °C for 50 min condition (CSF: 1.0), which had accounted for XOS purity of 76.5% based on the total sugar content of the liquid hydrolysate. In this condition, the sum of xylobiose, xylotriose, and xylotetraose which has a low degree of polymerization (DP) of 2 to 4 was determined as 80.6% of the total XOS. The highest XOS production score established using parameters including the xylan extraction ratio, XOS purity, and low DP XOS ratio was 5.7 at CSF 1.0 condition. XOS production score evaluated using the CSF is expected to be used as a productivity indicator of XOS in the industry (R-squared value: 0.92).


BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 4052-4063
Author(s):  
Haiyan Yang ◽  
Yuanchen Zhu ◽  
Yan Jin ◽  
Fuhou Lei ◽  
Zhengjun Shi ◽  
...  

Pseudo-lignin derived from the condensation of carbohydrate degradation products can retard the bioconversion of lignocellulose. In this work, liquid hot water (150 to 190 °C) and 1% H2SO4 pretreatments (130 to 190 °C) were used on sugarcane bagasse holocellulose for 3 h to generate pseudo-lignin. The effects of pseudo-lignin generation on structural characteristics and bioconversion of substrates were evaluated. The results showed that the formation of pseudo-lignin increased the hydrophobicity of the substrates. After LHW pretreatments and acid pretreatments at low temperatures (<150 °C), most of the xylans were removed, yielding 2.1 to 5.4% pseudo-lignin. Increasing acid pretreatment temperature to 170 and 190 °C yielded 34.3% and 93.6% pseudo-lignin, respectively. After pretreatment, the accessibilities and bioconversions of substrates were enhanced by degradation of xylans, increasing glucose conversions and bioethanol productions of substrates from 53.2 to 85.3%, and 9.9 to 13.1 g/L, respectively. However, large amounts of pseudo-lignin were generated during acid pretreatments at 170 °C, reducing glucose conversion and bioethanol yield to 45.6% and 6.3 g/L, respectively.


2014 ◽  
Vol 57 ◽  
pp. 141-149 ◽  
Author(s):  
Leandro Vinícius Alves Gurgel ◽  
Maria Teresa Borges Pimenta ◽  
Antonio Aprigio da Silva Curvelo

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