Antimicrobial Metabolites against Methicillin-Resistant Staphylococcus aureus from the Endophytic Fungus Neofusicoccum australe

Antimicrobial bioassay-guided fractionation of the endophytic fungi Neofusicoccum australe led to the isolation of a new unsymmetrical naphthoquinone dimer, neofusnaphthoquinone B (1), along with four known natural products (2–5). Structure elucidation was conducted by nuclear magnetic resonance (NMR) spectroscopic methods, and the antimicrobial activity of all the natural products was investigated, revealing 1 to be moderately active towards methicillin-resistant Staphylococcus aureus (MRSA) with a minimum inhibitory concentration (MIC) of 16 µg/mL.

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Synthesis of 1ʹ-acetoxychavicol acetate (ACA) analogues and their inhibitory activities against methicillin-resistant Staphylococcus aureus

Journal of Physical Science ◽

10.21315/jps2020.31.3.8 ◽

2020 ◽

Vol 31 (3) ◽

pp. 101-111

Author(s):

Nursyazwani Anuar ◽

◽

Mohamad Nurul Azmi Mohamad Taib ◽

Khayriyyah Mohd Hanafiah ◽

Aemen Ali Kudayr Al Shammary ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Moderate Activity ◽

Methicillin Resistant ◽

Inhibitory Activities ◽

Inhibitory Agents ◽

Mic Value

A series of 1ʹ-acetoxychavicol acetate analogues were synthesised and evaluated for their antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) using broth microdilution technique. The minimum inhibitory concentration (MIC) was used to determine whether the compounds had potential as inhibitory agents against the MRSA ATCC 43300, and the compounds with antimicrobial potential (<2000 μg ml–1) were tested for minimum bactericidal concentration (MBC). Based on this assay, compound 1 exhibited potent antimicrobial activity with MIC value of 250 μg ml–1. Meanwhile, compounds 2 and 13 showed the moderate activity with MIC values of 500 μg ml–1, respectively.

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In Vitro Test: Antimicrobial Activity Potential From Ciplukan Fruit (Physalis minima L.) Extract in Methicillin-resistant Staphylococcus aureus (MRSA)

Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya ◽

10.30651/jqm.v4i2.3645 ◽

2020 ◽

Vol 4 (2) ◽

pp. 209

Author(s):

Muhammad Hanun Mahyuddin ◽

Arifa Mustika ◽

Gwenny Ichsan Prabowo ◽

Deby Kusumaningrum

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Minimum Inhibitory Concentration ◽

Methicillin Resistant Staphylococcus Aureus ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Test Tube ◽

In Vitro Test ◽

Fruit Extract ◽

Methicillin Resistant

Abstract In Indonesia, in 2006, the prevalence of infections due to MRSA was 23.5%. Physalis minima L. plants are known to have antimicrobial activity because they contain compounds withaferin A, which can induce programmed cell death. This research was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Ciplukan (Physalis minima L.) extract in Methicillin-resistant Staphylococcus aureus (MRSA) bacteria. Dilution test with Mueller-Hinton broth medium used for measuring the minimum inhibitory concentration (MIC). Ciplukan fruit extract was dissolved in distilled water, and poured into a test tube with a certain concentration (0.9 g/mL (90%); 0.3 g/mL (45%); 0.15 g/mL (22.5%); 0.075 g/mL (11.25%) and 0.0375 g/mL (5.625%). After being incubated for 24 hours, the bacteria in the test tube were plated on nutrient agar plates to determine the MBC. The MIC cannot be determined, because the medium in the dilution test tube is disturbed by the color of the extract so that turbidity cannot be observed. From the observations of the minimum bactericidal concentration, MBC of the Ciplukan (Physalis minima L.) fruit extract against MRSA was in the P1 tube or equivalent to 0.9 g / ml (90%).Keywords : Physalis minima L., Antimicrobial, Ciplukan, Methicillin-resistant Staphylococcus aureus, Withaferin ACorrespondence : [email protected]

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