scholarly journals Diverse Banana Pseudostems and Rachis Are Distinctive for Edible Carbohydrates and Lignocellulose Saccharification towards High Bioethanol Production under Chemical and Liquid Hot Water Pretreatments

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3870
Author(s):  
Jingyang Li ◽  
Fei Liu ◽  
Hua Yu ◽  
Yuqi Li ◽  
Shiguang Zhou ◽  
...  
Keyword(s):  
Soluble Sugars ◽  
Enzymatic Saccharification ◽  
Hot Water ◽  
Biofuel Production ◽  
Bioenergy Crops ◽  
Bioethanol Production ◽  
Factors Affecting ◽  
Liquid Hot Water ◽  
Biomass Processing ◽  
Banana Crops

Banana is a major fruit crop throughout the world with abundant lignocellulose in the pseudostem and rachis residues for biofuel production. In this study, we collected a total of 11 pseudostems and rachis samples that were originally derived from different genetic types and ecological locations of banana crops and then examined largely varied edible carbohydrates (soluble sugars, starch) and lignocellulose compositions. By performing chemical (H2SO4, NaOH) and liquid hot water (LHW) pretreatments, we also found a remarkable variation in biomass enzymatic saccharification and bioethanol production among all banana samples examined. Consequently, this study identified a desirable banana (Refen1, subgroup Pisang Awak) crop containing large amounts of edible carbohydrates and completely digestible lignocellulose, which could be combined to achieve the highest bioethanol yields of 31–38% (% dry matter), compared with previously reported ones in other bioenergy crops. Chemical analysis further indicated that the cellulose CrI and lignin G-monomer should be two major recalcitrant factors affecting biomass enzymatic saccharification in banana pseudostems and rachis. Therefore, this study not only examined rich edible carbohydrates for food in the banana pseudostems but also detected digestible lignocellulose for bioethanol production in rachis tissue, providing a strategy applicable for genetic breeding and biomass processing in banana crops.

scholarly journals Effect of Dilute Acid and Alkaline Pretreatment of Typha australis (Typha Grass) for Bioethanol Production

2018 ◽  
pp. 1-6
Author(s):  
Bala, Auwalu ◽  
Farouq, Ahmad Ali ◽  
Ibrahim, Aliyu Dabai ◽  
Muhammad, Chika
Keyword(s):  
Enzymatic Saccharification ◽  
Reducing Sugar ◽  
Hot Water ◽  
Dilute Acid Pretreatment ◽  
Sugar Concentration ◽  
Bioethanol Production ◽  
Dilute Acid ◽  
Liquid Hot Water ◽  
Typha Australis ◽  
Reducing Sugar Concentration

Typha australis (Typha grass) obtained from Kware Lake was used in this research to produce bioethanol. Different pretreatment methods including dilute acid (0.2M H2SO4), dilute alkaline (0.2M NaOH) and liquid hot water pretreatments were used to pretreat the Typha grass sample before enzymatic saccharification for 7 days using Aspergillus niger isolated from soil sediment and the hydrolysate was seeded with Saccharomyces cerevisiae isolated from palm wine to produce bioethanol. HPLC was used to analyze bioethanol product. The result showed that pretreatment with 0.2M H2SO4 removed more hemicelluloses (7.0%) when compared with other pretreatment methods used, but pretreatment with 0.2M NaOH and liquid hot water removed more lignin (14.29%) than dilute acid pretreatment. The highest percentage reducing sugar concentration of 0.58% was obtained from lower part of the sample pretreated with liquid hot water while Typha grass pretreated with 0.2M H2SO4 and 0.2M NaOH produced the highest percentage reducing sugar concentration of 0.32% each from the upper part of the sample. Also, the highest Bioethanol concentration of 2.07% was obtained at day 6 of fermentation from the Typha grass pretreated with liquid hot water while Typha grass pretreated with 0.2M H2SO4 and 0.2M NaOH produced highest Bioethanol concentration of 0.43% and 0.54% respectively. The results indicate that Typha grass can be harnessed for bioethanol production thereby reducing their negative impact on Lakes.

Changes in poplar (Populus trichocarpa) wood porous structure after liquid hot water (LHW) pretreatment

2020 ◽  
Vol 112 ◽  
pp. 71-78
Author(s):  
Florentyna Akus-Szyblerg ◽  
Jan Szadkowski ◽  
Andrzej Antczak ◽  
Janusz Zawadzki
Keyword(s):  
Enzymatic Hydrolysis ◽  
Porous Structure ◽  
Populus Trichocarpa ◽  
Hot Water ◽  
Size Exclusion ◽  
Bioethanol Production ◽  
Poplar Wood ◽  
Liquid Hot Water ◽  
Exclusion Chromatography ◽  
Pretreatment Conditions

Changes in poplar (Populus trichocarpa) wood porous structure after liquid hot water (LHW) pretreatment. The aim of this research was to investigate the effect of applying different hydrothermal pretreatment conditions on the porous structure of poplar wood. Porosity is recognised as an important factor considering efficiency of an enzymatic hydrolysis as a step of bioethanol production. Native poplar wood as well as solid fractions after pretreatment performed at different temperatures (160 °C, 175 °C and 190 °C) were analysed. Porous structure was examined with an inverse size-exclusion chromatography (ISEC) method. Results indicated a significant development of the porous structure of the biomass with increasing porosity along with the growing temperature of the LHW process. The temperature of 190 °C was chosen as the most promising condition of poplar wood LHW pretreatment in terms of the efficiency of the subsequent steps of bioethanol production. The obtained results were consistent with the previous experimental data procured during analysis of the LHW pretreated poplar wood and its subsequent enzymatic hydrolysis yield.

Combined liquid hot water with sodium carbonate-oxygen pretreatment to improve enzymatic saccharification of reed

2020 ◽  
Vol 297 ◽  
pp. 122498 ◽  
Author(s):  
Fei Xia ◽  
Jingwei Gong ◽  
Jie Lu ◽  
Yi Cheng ◽  
Shangru Zhai ◽  
...  
Keyword(s):  
Sodium Carbonate ◽  
Enzymatic Saccharification ◽  
Hot Water ◽  
Liquid Hot Water

Inhibitory compounds formation after liquid hot water (LHW) pretreatment of corn stover as an alternative to wood lignocellulosic feedstock for bioethanol production

2020 ◽  
Vol 110 ◽  
pp. 110-117
Author(s):  
Florentyna Akus-Szylberg ◽  
Andrzej Antczak ◽  
Janusz Zawadzki
Keyword(s):  
Corn Stover ◽  
Hot Water ◽  
Bioethanol Production ◽  
Spectrophotometric Methods ◽  
Liquid Hot Water ◽  
Treatment Conditions ◽  
Lignocellulosic Feedstock ◽  
Inhibitory Compounds ◽  
Different Temperatures ◽  
Mixed Biomass

Inhibitory compounds formation after liquid hot water (LHW) pretreatment of corn stover as an alternative to wood lignocellulosic feedstock for bioethanol production. Thus far, corn stover has been perceived as a promising lignocellulosic alternative to wood intended for bioethanol procurement, however it should be recognised also as a potential future component in a mixed biomass system. The aim of this research was to investigate the effect of applying different hydrothermal treatment conditions on the potential inhibitory compounds formation from corn stover. An analysis of selected inhibitory compounds formed after pretreatment performed at different temperatures (160°C, 175°C, 190°C and 205°C) was carried out. Furfural, simple sugars and lignin were some of the inhibitors examined with HPLC and UV-VIS spectrophotometric methods. Furthermore, the chemical composition of organic extracts obtained from native and LHW pretreated biomass was analyzed qualitatively with GC-MS method and inhibitory compounds like vanillin, sitosterol or syringol were detected. As a result of those investigations compared to enzymatic hydrolysis yield the temperature of 175°C was chosen as the most promising condition of corn stover LHW pretreatment in terms of the efficiency of the subsequent phases of bioethanol production.

scholarly journals High consistency enzymatic saccharification of sweet sorghum bagasse pretreated with liquid hot water

2012 ◽  
Vol 108 ◽  
pp. 252-257 ◽  
Author(s):  
Wen Wang ◽  
Xinshu Zhuang ◽  
Zhenhong Yuan ◽  
Qiang Yu ◽  
Wei Qi ◽  
...  
Keyword(s):  
Sweet Sorghum ◽  
Enzymatic Saccharification ◽  
Hot Water ◽  
Sweet Sorghum Bagasse ◽  
Liquid Hot Water ◽  
Sorghum Bagasse ◽  
High Consistency

scholarly journals Enzymatic Saccharification and Ethanol Fermentation of Reed Pretreated with Liquid Hot Water

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Jie Lu ◽  
XueZhi Li ◽  
Jian Zhao ◽  
Yinbo Qu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Saccharification ◽  
Hot Water ◽  
Ethanol Conversion ◽  
Optimum Conditions ◽  
Enzymatic Digestibility ◽  
Material Source ◽  
Theoretical Yield ◽  
Liquid Hot Water ◽  
Filter Paper Unit

Reed is a widespread-growing, inexpensive, and readily available lignocellulosic material source in northeast China. The objective of this study is to evaluate the liquid hot water (LHW) pretreatment efficiency of reed based on the enzymatic digestibility and ethanol fermentability of water-insoluble solids (WISs) from reed after the LHW pretreatment. Several variables in the LHW pretreatment and enzymatic hydrolysis process were optimized. The conversion of glucan to glucose and glucose concentrations are considered as response variables in different conditions. The optimum conditions for the LHW pretreatment of reed area temperature of 180°C for 20min and a solid-to-liquid ratio of 1 : 10. These optimum conditions for the LHW pretreatment of reed resulted in a cellulose conversion rate of 82.59% in the subsequent enzymatic hydrolysis at 50°C for 72 h with a cellulase loading of 30 filter paper unit per gram of oven-dried WIS. Increasing the pretreatment temperature resulted in a higher enzymatic digestibility of the WIS from reed. Separate hydrolysis and fermentation of WIS showed that the conversion of glucan to ethanol reached 99.5% of the theoretical yield. The LHW pretreatment of reed is a suitable method to acquire a high recovery of fermentable sugars and high ethanol conversion yield.

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