Intergenerational Transmission of Resistance of Callosobruchus maculatus to Essential Oil Treatment

Due to the rise of numerous legal restrictions as well as the increasing emergence of resistant populations, the number of available pesticides is decreasing significantly. One of the potential alternatives often described in the literature are essential oils (EOs). However, there is a lack of research addressing the potential emergence of resistance to this group of substances. In this paper, we investigated the multi-generational effects of sublethal concentrations of rosemary oil (Rosmarinus officinalis) on physiological and biochemical parameters of the cowpea weevil (Callosobruchus maculatus) such as egg laying, hatchability, oxygen consumption and acetylcholinesterase activity. Imago, which as larvae were exposed to EO at concentrations equivalent to LC25, showed significantly lower mortality. The results obtained indicate the potential development of resistance in insects exposed to EO in concentrations corresponding to LC25. In addition, in the case of the group treated with an EO concentration corresponding to LC3.12, a stimulation effect of the above-mentioned parameters was observed, which may indicate the occurrence of a hormesis effect. The obtained results may be an important reference for the development of future guidelines and EO-based insecticides.

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Adult time-mortality response and changes in reproductive attributes in Callosobruchus maculatus (Fab.) following UV irradiation

Journal of Life and Earth Science ◽

10.3329/jles.v5i0.7343 ◽

1970 ◽

Vol 5 ◽

pp. 17-22 ◽

Cited By ~ 1

Author(s):

M Saiful Islam ◽

Sharmin Mustari

Keyword(s):

Uv Irradiation ◽

Callosobruchus Maculatus ◽

Adult Emergence ◽

Egg Laying ◽

Adult Longevity ◽

Cowpea Weevil ◽

Mortality Response ◽

Key Words Uv Irradiation ◽

F1 Generation ◽

Uv Rays

Using 254nm UV radiations of exposure periods from 2 to 16 min against adults. Time mortality response of the cowpea weevil, Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae), adults and alterations in vital reproductive attributes as fecundity, immature duration, adult emergence and adult longevity of the beetles from parental through F1 generation have been estimated. Time mortality response of the adults of the cowpea weevil Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae), and alternations in vital reproductive attributes is fecundity, immature duration, adult emergence and longevity from parental through F1 generation using 254nm UV radiations of response periods from 2-16min have been estimated. Results show that an exposure time to kill 50% of the adults is around 20 min (LT50 = 19.99min), and irradiation significantly reduced egg-laying (P<0.001), lengthened immature durations (i.e. larval and pupal developmental periods; P<0.05), decreased adult emergences as well as longevity in both sexes (P<0.001) in the parental generation. The effects of the UV-rays on F1 progenies, however, were less pronounced than that expressed in the parental generation, but the immature duration was significantly increased (P<0.01) but the longevity in both sexes was significantly reduced (P<0.001). Relevance of this study in relation to prospective phytosanitary treatments of the stored pulses with UV-rays has been discussed. Key words: UV irradiation; Callosobruchus maculatus; time-mortality response; reproductive attributes; stored pulses DOI: 10.3329/jles.v5i0.7343 J. Life Earth Sci., Vol. 5: 17-22, 2010

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Structure-Reactivity Relationships on Substrates and Inhibitors of the Lysine Deacylase Sirtuin 2 from Schistosoma Mansoni (SmSirt2)

10.26434/chemrxiv.7957544 ◽

2019 ◽

Cited By ~ 1

Author(s):

Daria Monaldi ◽

Dante Rotili ◽

Julien Lancelot ◽

Martin Marek ◽

Nathalie Wössner ◽

...

Keyword(s):

Schistosoma Mansoni ◽

Human Cancer ◽

Egg Laying ◽

Human Cancer Cells ◽

Parasite Survival ◽

Survival And Reproduction ◽

Emergence Of Resistance ◽

First Time ◽

Parasitic Life Cycle

The only drug for treatment of Schistosomiasis is Praziquantel, and the possible emergence of resistance makes research on novel therapeutic agents necessary. Targeting of Schistosoma mansoni epigenetic enzymes, which regulate the parasitic life cycle, emerged as promising approach. Due to the strong effects of human Sirtuin inhibitors on parasite survival and reproduction, Schistosoma sirtuins were postulated as therapeutic targets. In vitro testing of synthetic substrates of S. mansoni Sirtuin 2 (SmSirt2) and kinetic experiments on a myristoylated peptide demonstrated lysine long chain deacylation as an intrinsic SmSirt2 activity for the first time. Focused in vitro screening of the GSK Kinetobox library and structure-activity relationships (SAR) of identified hits, led to the first SmSirt2 inhibitors with activity in the low micromolar range. Several SmSirt2 inhibitors showed potency against both larval schistosomes (viability) and adult worms (pairing, egg laying) in culture without general toxicity to human cancer cells.<br>

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Evaluation of the host preference and life history of Callosobruchus maculatus (F.) (Cowpea weevil) (Coleoptera: Chrysomelidae) on stored pulses

Pure and Applied Biology ◽

10.19045/bspab.2020.90231 ◽

2020 ◽

Vol 9 (4) ◽

Author(s):

Nosheen Jehajo

Keyword(s):

Life History ◽

Host Preference ◽

Callosobruchus Maculatus ◽

Cowpea Weevil ◽

History Of

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Use of Microwave Heating Technology for Disinfestation of Stored Green Gram (Vigna radiata) against Cowpea Bruchid Callosobruchus maculatus (Fabricius)

Legume Research - An International Journal ◽

10.18805/lr-4653 ◽

2021 ◽

Author(s):

R. Kooner ◽

D.K. Sharma ◽

K.S. Suri

Keyword(s):

Callosobruchus Maculatus ◽

Pupal Stage ◽

Exposure Period ◽

Green Gram ◽

Life Stages ◽

Chemical Methods ◽

Cowpea Weevil ◽

Alternative Approach ◽

Cowpea Bruchid ◽

Heating Technology

Background: Pulses are an integral component of food in India which acts as major source of protein required for growth and maintenance of body. But during their storage they suffer heavy losses resulting in depletion of their quality and edibility. The present studies focused on disinfestation of green gram grains using microwaves as an alternative approach to chemical methods for controlling insects in grains and pulses. Methods: In this laboratory studies during 2017-18, the grains of green gram were infested with different life stages of the cowpea weevil and exposed to 200, 400, 600 and 800 W microwave power levels, each at an exposure period of 10, 20, 30 and 40 s. Result: Eggs were the most susceptible, while pupal and adults were the least susceptible life stages to microwave treatments. Complete mortality of eggs was achieved with microwave treatments (400, 600 and 800 W), each at an exposure period of 30 and 40 s. Complete inhibition of larval stage was attained with 400 and 600 W (30 and 40 s) and 800 W (20, 30 and 40 s exposure) while for pupal stage it was observed at the higher wattages of 600 (40 s exposure) and 800 (30 and 40 s exposure). Microwave wattage of 600 W for 40 sand 800 W for 30 and 40 s exposure caused 100% mortality of 2d old adults of cowpea bruchid.

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Determination of impact of Azadirachta indica L. leaves on adults and eggs of Callosobruchus maculatus Fabricius, the largest predator of stored cowpea (Vigna unguiculata Walp) by an applicable method by farmers

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.4(5).p215-221 ◽

2015 ◽

Vol 4 (5) ◽

pp. 215-221

Author(s):

Ablaye Faye ◽

Malick Sarr ◽

Abdoulaye Samb ◽

Cheikh Thiaw ◽

MbackÃ© SembÃ¨ne

Keyword(s):

Mortality Rate ◽

Callosobruchus Maculatus ◽

Maximum Effect ◽

Maximal Effect ◽

Cowpea Weevil ◽

Lethal Effects ◽

Neem Leaves ◽

Leaf Powder ◽

Higher Doses

The effect of neem leaves has been tested in the laboratory on eggs and adults of cowpea weevil (C. maculatus). Different formulations of this plant were applied to these forms of C. maculatus Fab. Grinding fresh contact sheets induced significant lethal effects from 96.12% to 100% on eggs; whereas 100% of mortality was recorded at the end of eight days of applica-tion to three adults with higher doses. Fumigation on turn proved less effec-tive than contact on eggs. It induced a maximal effect of 95.73% mortality with the larger dose (D4: 0.02912g/cm3). On adults, we recorded highest mortality (100%) from the 7th day of the show with the highest dose. The aqueous extract of neem leaf powder was less effective than all other formu-lations on the eggs as well as adults of this insect; with a higher mortality rate (74.99%) observed on the eggs with the application of the concentration C2. On adults we recorded a maximum effect (100% mortality) from the 13th day of the application with the highest concentration (C1). These mortalities would be related to the support of several active molecules contained in neem as established in literature.

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Recrudescence ofPlasmodium malariaeafter Quinine

Case Reports in Medicine ◽

10.1155/2014/590265 ◽

2014 ◽

Vol 2014 ◽

pp. 1-3 ◽

Cited By ~ 4

Author(s):

Irfanali R. Kugasia ◽

Farhana K. Polara ◽

Hussein Assallum

Keyword(s):

Case Reports ◽

Plasmodium Malariae ◽

Sub Saharan Africa ◽

Case Patient ◽

Liver Stage ◽

Literature Research ◽

Development Of Resistance ◽

Sub Saharan ◽

Emergence Of Resistance

Plasmodium malariaecauses uncommon benign malaria found in the malaria endemic regions mostly of Sub-Saharan Africa. AsPlasmodium malariaedoes not have a continued liver stage in humans the only way to have reinfection without reexposure is through recrudescence. However, reports of its recrudescence after antimalarials are rare with only a handful of case reports in the literature. Research in this field to date has not been able to establish definitively an emergence of resistance inPlasmodium malariaeto commonly used antimalarials. In the presented case, patient had a recrudescence ofP. malariaeafter full treatment with quinine and clindamycin. This recrudescence was treated with full course of chloroquine with clearance of parasite from blood immediately after treatment and at two months’ follow up. The recrudescence in this case cannot be explained by mechanisms explained in prior articles. We propose that the indolence of some of thePlasmodium malariaetrophozoites in the blood can shield them from the effect of the toxic effects of antimalarials and enable them to produce recrudescence later. However, when recrudescence happens, this should not be considered a case of development of resistance and a course of chloroquine should be considered.

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