scholarly journals Separation and Determination of Chemopreventive Phytochemicals of Flavonoids from Brassicaceae Plants

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4734
Author(s):  
Sylwia Bajkacz ◽  
Magdalena Ligor ◽  
Irena Baranowska ◽  
Bogusław Buszewski
Keyword(s):  
Thin Layer ◽  
Chinese Cabbage ◽  
Plant Extracts ◽  
Free Form ◽  
Dpph Assay ◽  
Brussels Sprouts ◽  
Pak Choi ◽  
Total Antioxidant ◽  
Layer Chromatography

The main aim of this study was to develop a method for the isolation and determination of polyphenols—in particular, flavonoids present in various morphological parts of plants belonging to the cabbage family (Brassicaceae). Therefore, a procedure consisting of maceration, acid hydrolysis and measurement of the total antioxidant capacity of plant extracts (using DPPH assay) was conducted. Qualitative analysis was performed employing thin-layer chromatography (TLC), which was presented to be a suitable methodology for the separation and determination of chemopreventive phytochemicals from plants belonging to the cabbage family. The study involved the analysis of 25 vegetal samples, including radish, broccoli, Brussels sprouts, kale, canola, kohlrabi, cabbage, Chinese cabbage, red cabbage, pak choi and cauliflower. In addition, selected flavonoids content in free form and bonded to glycosides was determined by using an RP-UHPLC-ESI-MS/MS method.

Thin-Layer Chromatography for Chlorinated Pesticide Residue Analysis without Cleanup

1964 ◽  
Vol 47 (2) ◽  
pp. 306-310
Author(s):  
H V Morley ◽  
M Chiba
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extracts ◽  
Residue Analysis ◽  
Rapid Screening ◽  
Pesticide Residue Analysis ◽  
Cleanup Procedure ◽  
Layer Chromatography

Abstract Thin-layer chromatography on silica gel may be used for the determination of chlorinated pesticide residues in some plant extracts without prior cleanup, thus eliminating the possible loss of pesticides during the cleanup procedure. Rapid screening of a large number of samples is thus possible with consequent saving of time and labor. When necessary, thin-layer chromatography may be used as a prior cleanup for gas chromatography.

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