scholarly journals Antioxidant Potential of Flower Extracts from Centaurea spp. Depends on Their Content of Phenolics, Flavonoids and Free Amino Acids

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7465
Author(s):  
Piotr Salachna ◽  
Anna Pietrak ◽  
Łukasz Łopusiewicz
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Reducing Sugars ◽  
Total Phenolics ◽  
Raw Materials ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Strong Positive Correlation ◽  
Important Health

Scientists intensely search for new sources of antioxidants, perceived as important health-promoting agents. Some species of the large genus Centaurea provide raw materials for the pharmaceutical and cosmetic industries, as well as produce edible flowers. This is the first study that determines the content of total polyphenols, flavonoids, reducing sugars, free amino acids and the antioxidant potential in the flower extracts of C. nigra L., C. orientalis L. and C. phrygia L. The total polyphenol and flavonoid content is the highest in the extract of C. orientalis, and the lowest in that of C. phrygia. Similarly, C. orientalis shows the greatest scavenging activity on DPPH (1,1-diphenyl-2-picryl-hydrazyl), ABTS [2,2′-azobis(3-ethylbenzothiazoline-6-sulfonate)] and Fe3+ reducing power assays, whereas the lowest activity is found for C. phrygia. The highest content of reducing sugars is found in C. nigra, while C. orientalis has the highest levels of free amino acids. We find a strong positive correlation between total phenolics and flavonoids and the antioxidant capacity of all three Centaurea species. Moreover, the content of free amino acids strongly and positively correlates with the levels of total phenolics and flavonoids, antioxidant activity assessed by DPPH and ABTS assays and Fe3+ reducing power. Summing up, C. orientalis exhibits the strongest antioxidant potential of the investigated Centaurea species. This species could potentially be a natural source of antioxidant substances for the pharmacy, cosmetics and food industries. The content of free amino acids may be used as a marker of the antioxidant status of Centaurea species.

scholarly journals Study of Epilobium angustifolium L. amino acids content by HPLC method

2021 ◽  
pp. 85-90
Author(s):  
Halyna Feshchenko ◽  
Oleksandra Oleshchuk ◽  
Liudmyla Slobodianiuk ◽  
Ivanna Milian
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Raw Materials ◽  
Hplc Method ◽  
Raw Material ◽  
Epilobium Angustifolium ◽  
Amino Acids Composition

The use of plant raw materials is one of the areas of modern pharmaceutical science in the production of herbal drugs. The genus Epilobium counts more than 200 species, many species of which are used in traditional medicine. Among the Epilobium species, Epilobium angustifolium is one of the well-known medicinal plants which have been used worldwide in habitual medicine. There is insufficient information in the literature on the biologically active substances of Epilobium angustifolium L. The presence of three major polyphenol groups: phenolic acids, flavonoids, and ellagitannins were identified in E. angustifolium extracts. Traditionally, the infusion of leaves of this plant could be useful for headaches, cold and gastrointestinal disorder. The Epilobium angustifolium L. as an insufficiently studied plant is a promising object of study, including amino acids composition. To assess the relationship between the production of primary metabolites and their possible therapeutic properties, we analyzed the amino acid profile of the plant Epilobium angustifolium used in traditional medicine. The study of compounds generated by plants as a result of defense mechanisms permits an understanding of the molecular mechanism involved in their medicinal properties. The aim. Thus, the aim of the study was to conduct an HPLC analysis of the amino acids of E. angustifolium to establish the prospects for the use of the raw materials in medical and pharmaceutical practice. The results of the current study will be used in further breeding programs aimed to obtain an industrial form of E. angustifolium suitable for pharmaceutical and food applications. Materials and methods. The determination of amino acids composition of Epilobium angustifolium was conducted using Agilent 1200 (Agilent Technologies, USA). Results. The HPLC method identified sixteen free amino acids and seventeen bound amino acids in the Epilobium angustifolium herb. The studies have shown that Epilobium angustifolium L. herb is mainly composed of free amino acids such as L-phenylalanine (1.65 µg/mg), L-glutamic acid (1.51 µg/mg), L-arginine (1.24 µg/mg), L-alanine (0.98 µg/mg) and L-aspartic acid (0.57 µg/mg), which were presents in the greatest amount. The dominant bound amino acids in the studied raw material were L-glutamic acid, L-aspartic acid, L-leucine, and L-alanine, the content of which was 32.37 µg/mg, 10.59 µg/mg, 8.70 µg/mg, and 6.22 µg/mg respectively. Conclusions. Using the HPLC method determined the amino acids in the herb of Epilobium angustifolium L. The concentrations of L-aspartic acid, L-glutamic acid, L-arginine, L-alanine and L-phenylalanine are predominate among free and bound amino acids in the Epilobium angustifolium L. herb. The result shows that Epilobium angustifolium L. is the source of amino acids, so the use of this plant raw material for new remedies is possible in the future

scholarly journals Onion Extract Application Effects on Flowering Behavior and Yield, and a few Chemical Constituents of Shoots throughout Dormancy Break in "Anna" Apple Trees

2019 ◽  
Vol 7 ◽  
pp. 1-15
Author(s):  
M.A. Seif El-Yazal ◽  
Samir A. Seif El-Yazal ◽  
Mohamed E. Morsi ◽  
Mostafa M. Rady
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Reducing Sugars ◽  
Fruit Yield ◽  
Bud Break ◽  
Total Sugars ◽  
Onion Extract ◽  
Total Carbohydrates ◽  
Total Free Amino Acids

Metabolic changes in water content, total carbohydrates, total sugars, reducing sugars, anthocyanin, total free amino acids, proline and total indoles in shoots of "Anna" apple (Malus sylvestris, Mill) trees were investigated during dormancy and bud break under the effect of foliar application of four onion extract rates (5, 10, 15 and 20%). Flowering percentage and fruit yield characteristics (i.e., fruit-set, number of fruits tree-1 and fruit yield tree-1) were increased, while number of days recorded to reach full bloom were reduced with the application of both onion extract rates (15 or 20%). In addition, contents of total carbohydrates, total sugars, reducing sugars, anthocyanin, total free amino acids, proline and total indoles in shoots were significantly increased in floral buds, particularly during bud break. In contrast, the content of free phenols was reduced. The best results were obtained from the rate of onion extract at the level of 20% followed by 15% that could be recommended for early and high percentage of flowering and increased yield by regulating the metabolism of amino acids, including proline and indoles in apple floral buds.

scholarly journals Biogenic amines in rennet ripening cheeses as a health risk to consumers

2017 ◽  
Vol 73 (4) ◽  
pp. 214-219 ◽  
Author(s):  
Anna Madejska ◽  
Mirosław Michalski ◽  
Jacek Osek
Keyword(s):  
Amino Acids ◽  
Biogenic Amines ◽  
Free Amino Acids ◽  
Free Amino ◽  
Raw Materials ◽  
Food Poisoning ◽  
Storage Conditions ◽  
Detection Methods ◽  
Temperature Time

Biogenic amines are nitrogen compounds which are products of the decarboxylation of free amino acids. They are produced with the participation of bacterial microflora producing enzymes, and they may be introduced together with food into the human body. The highest amounts of biogenic amines are found in meat, fish and cheeses. Consumption of products containing biogenic amines can cause food poisoning and allergies in consumers. The most common amines in cheeses are tyramine, histamine, putrescine, cadaverine and 2-phenylethylamine. The formation of amines depends on the technology of food production, storage conditions (temperature, time, pH, moisture), the quality of the raw materials (the content of free amino acids, proteins, salts, sugars) and the presence of microorganisms producing decarboxylases. This article describes different types of biogenic amines, their formation, detection methods and health risks to consumers. Information on the harmfulness of biogenic amines and on factors conducive to their production may help prevent poisoning with these compounds.

scholarly journals HPLC ANALYSIS OF AMINO ACIDS CONTENT IN CRAMBE CORDIFOLIA AND CRAMBE KOKTEBELICA LEAVES

2021 ◽  
pp. 111-116
Author(s):  
LIUDMYLA SLOBODIANIUK ◽  
LILIIA BUDNIAK ◽  
SVITLANA MARCHYSHYN ◽  
OLHA SKRYNCHUK ◽  
VICTORIIA KUDRIA
Keyword(s):  
Experimental Data ◽  
Amino Acids ◽  
Glutamic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Hplc Analysis ◽  
Raw Materials ◽  
Hplc Method ◽  
Primary Metabolites ◽  
Digestible Amino Acids

Objective: The aim of our study was to establish the content of some primary metabolites, such as amino acids in Crambe cordifolia and Crambe koktebelica. The lack of experimental data induced us to determine these compounds. Methods: Crambe cordifolia and Crambe koktebelica leaves were selected as the objects of the study. The amino acids in the raw materials were determined by the HPLC method. Results: The results of the research revealed that the leaves of Crambe cordifolia and Crambe koktebelica contain fifteen and sixteen free amino acids respectively. Among the free amino acids L-histidine was presented in Crambe cordifolia leaves in the greatest amount, its content was 12.19 µg/mg. The content of free L-arginine, L-valine, L-phenylalanine, L-isoleucine was the greatest in Crambe koktebelica leaves, it was 2.23 µg/mg, 2.04 µg/mg, 1.74 µg/mg, 1.50 µg/mg respectively. The content of bound L-glutamic acid, Glycine, L-arginine, L-leucine was the highest in Crambe cordifolia and Crambe koktebelica leaves. Conclusion: The results of the study showed that Crambe cordifolia and Crambe koktebelica can be considered as a source of highly digestible amino acids that can be used to treat some diseases.

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