scholarly journals Onion Extract Application Effects on Flowering Behavior and Yield, and a few Chemical Constituents of Shoots throughout Dormancy Break in "Anna" Apple Trees

2019 ◽  
Vol 7 ◽  
pp. 1-15
Author(s):  
M.A. Seif El-Yazal ◽  
Samir A. Seif El-Yazal ◽  
Mohamed E. Morsi ◽  
Mostafa M. Rady

Metabolic changes in water content, total carbohydrates, total sugars, reducing sugars, anthocyanin, total free amino acids, proline and total indoles in shoots of "Anna" apple (Malus sylvestris, Mill) trees were investigated during dormancy and bud break under the effect of foliar application of four onion extract rates (5, 10, 15 and 20%). Flowering percentage and fruit yield characteristics (i.e., fruit-set, number of fruits tree-1 and fruit yield tree-1) were increased, while number of days recorded to reach full bloom were reduced with the application of both onion extract rates (15 or 20%). In addition, contents of total carbohydrates, total sugars, reducing sugars, anthocyanin, total free amino acids, proline and total indoles in shoots were significantly increased in floral buds, particularly during bud break. In contrast, the content of free phenols was reduced. The best results were obtained from the rate of onion extract at the level of 20% followed by 15% that could be recommended for early and high percentage of flowering and increased yield by regulating the metabolism of amino acids, including proline and indoles in apple floral buds.

Author(s):  
Mohamed A. Seif El-Yazal

The effect of garlic extract at different concentrations for reaching early break dormancy in buds of "Anna" apple (Malus sylvestris, Mill) trees and their effects on metabolic changes in the content of shoots from (water content %, total carbohydrates, total sugars, reducing sugars, anthocyanine, total free amino acids, free proline, total indoles and free phenoles) during their release from dormancy were investigated. The trees were grown in loamy sand soil, and sprayed with five treatments; 0, 5, 10, 15 and 20% garlic extract. Generally, it was found that all studied flowering parameters; date of flower buds break, percentage of bud’s break, fruit set, total number of fruits/tree, yield/tree (kg) and seasonal changes in some chemical constituents of shoots; water content %, total carbohydrates, total sugars, reducing sugars, anthocyanine, total free amino acids, free proline and total indoles were increased with the application of different treatments. The best results were obtained from the treatments of garlic extract at 15 % flowed by 20 %. On the contrary, the same treatments decreased free phenols in shoots as compared to the control. It could be recommended to use garlic extract at the rate of 15 % for improving bud break, growth, yield and chemical constituents of apple shoots


2014 ◽  
Vol 6 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Meenakshi Thakur ◽  
B.S. Sohal

The present investigation was aimed to assess the effect of different concentrations of elicitors viz., salicylic acid (SA) and benzothiadiazole (BTH) on physiomorphological and biochemical parameters of Brassica juncea and B. napus cultivars. The field experiment was conducted during 2012-13 at Punjab Agricultural University, Ludhiana, India. Four different treatments of elicitors and a fungicide treatment were given to 10 week old plants up to four consecutive weeks. Content of total soluble protein, free amino acids, total sugars and reducing sugars ofleaves of both B. juncea and B. napus were evaluated after each spray. The content of total soluble protein increased, whereas the contents of free amino acids, total sugars and reducing sugars decreased in all the treatments after each spray. Among all the treatments, the combinations of elicitors, i.e., BTH (3 ppm) + SA (33 ppm) and BTH (7 ppm) + SA (17 ppm) exhibited maximum contents of total soluble protein, free amino acids, total sugars and reducing sugars than control in both the cultivars. These treatments were more pronounced in increasing plant height, internodal distance and number of pods per plant in both the cultivars. Our results suggest that the combinations of elicitors act synergistically to promote growth and metabolic activities in B. juncea and B. napus cultivars leading to the induction and regulation of disease resistance.


2001 ◽  
Vol 68 (1) ◽  
pp. 117-129 ◽  
Author(s):  
ABDELGHANI OUMER ◽  
PILAR GAYA ◽  
ESTRELLA FERNÁNDEZ-GARCÍA ◽  
RAÚL MARIACA ◽  
SONIA GARDE ◽  
...  

Hispánico cheese, a semi-hard Spanish variety, was manufactured from a mixture of pasteurized cows' and ewes' milks (4[ratio ]1) using a commercial mesophilic LD-type starter comprising Lactococcus lactis subsp. cremoris, Lc. lactis subsp. lactis, Lc. lactis subsp. lactis var diacetylactis and Leuconostoc mesenteroides subsp. cremoris. Varying amounts (0–1·0 g/kg) of an Enterococcus faecalis INIA 4 culture in milk were added as a bacteriocin-producing adjunct. Differences in pH between cheeses manufactured with and without the bacteriocin producer did not exceed 0·11 pH units. Starter lactococci lost viability more rapidly in cheeses made with the bacteriocin producer, which reached counts of up to 6×107 cfu/g during ripening. Aminopeptidase activity in 1-d-old cheese made from milk inoculated with 1·0 g bacteriocin-producing culture/kg was twice that in control cheese. Degrees of overall proteolysis and levels of total free amino acids in 45-d-old cheese made with 1·0 g bacteriocin-producing culture/kg were 1·80-fold and 2·17-fold those in control cheese of the same age. Inoculating milk with 1·0 g/kg bacteriocin-producing culture reduced the level of hydrophobic peptides in the resultant cheese, increased the concentrations of 3-methyl-1-butanal, diacetyl and acetoin, and resulted in the highest scores for flavour quality and flavour intensity throughout ripening.


1969 ◽  
Vol 15 (9) ◽  
pp. 891-901 ◽  
Author(s):  
D W Palmer ◽  
T Peters

Abstract A simple automated method is described for determining the level of total free amino acids in the blood. The method utilizes the AutoAnalyzer, and is based on the formation of colored complexes by uniting free amino groups with 2,4,6-trinitrobenzene sulfonate (TNBS). Proteins do not interfere because the free amino acids are first separated by dialysis. Characteristics of the reaction and potential clinical applications of the procedure are discussed.


Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7465
Author(s):  
Piotr Salachna ◽  
Anna Pietrak ◽  
Łukasz Łopusiewicz

Scientists intensely search for new sources of antioxidants, perceived as important health-promoting agents. Some species of the large genus Centaurea provide raw materials for the pharmaceutical and cosmetic industries, as well as produce edible flowers. This is the first study that determines the content of total polyphenols, flavonoids, reducing sugars, free amino acids and the antioxidant potential in the flower extracts of C. nigra L., C. orientalis L. and C. phrygia L. The total polyphenol and flavonoid content is the highest in the extract of C. orientalis, and the lowest in that of C. phrygia. Similarly, C. orientalis shows the greatest scavenging activity on DPPH (1,1-diphenyl-2-picryl-hydrazyl), ABTS [2,2′-azobis(3-ethylbenzothiazoline-6-sulfonate)] and Fe3+ reducing power assays, whereas the lowest activity is found for C. phrygia. The highest content of reducing sugars is found in C. nigra, while C. orientalis has the highest levels of free amino acids. We find a strong positive correlation between total phenolics and flavonoids and the antioxidant capacity of all three Centaurea species. Moreover, the content of free amino acids strongly and positively correlates with the levels of total phenolics and flavonoids, antioxidant activity assessed by DPPH and ABTS assays and Fe3+ reducing power. Summing up, C. orientalis exhibits the strongest antioxidant potential of the investigated Centaurea species. This species could potentially be a natural source of antioxidant substances for the pharmacy, cosmetics and food industries. The content of free amino acids may be used as a marker of the antioxidant status of Centaurea species.


AGROFOR ◽  
2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Ayako SEKIYAMA ◽  
Kiyohisa SATO ◽  
Sawahiko SHIMADA ◽  
Tomonori FUJIKAWA

The optimal harvest season of Japanese green tea in Japan is judged by individual farmers based on their experiences. To confirm that optimal season, it requires a lot of efforts such as sampling of tea flushes by plucking, chemical component measuring by near-infrared spectroscopic analysis. Thus, an efficient method to determine the optimal season which can cover a wide area at once is considered highly helpful for farmers. This research measured normalized difference vegetation index (NDVI) of tea flushes by a multi-spectrum sensor mounted on an unmanned aerial vehicle. At the same time, chemical component of tea flushes was measured to compare with the result of NDVI. The result showed that the value of NDVI for the optimal harvest season of tea-leaf is 0.6 to 0.7. The result of chemical component measuring showed the increase of total free amino acids and reduction of total free sugars while tea-leaf ripens. Therefore, there was a positive relation between the value of NDVI, and total free amino acids and total free sugars. It indicates a high possibility to predict the optimal harvest season of tea flushes by NDVI.


2020 ◽  
Vol 12 (2) ◽  
pp. 433-446
Author(s):  
El-Anwar OSMAN ◽  
Wedad A. KASIM ◽  
Nabil A. OMAR ◽  
Samar E. SALAMA

In a greenhouse experiment, the inoculated and uninoculated grains with Azospirillum brasilense NO40 or Stenotrophomonas maltophilia were sown in unsterilized sandy soil and watered normally till the 8th day. Thereafter, the drought stress was initiated by watering pots once every 10 days while the unstressed pots were irrigated normally once every 5 days. Samples of spikes and dry grains were collected after 120 days from sowing. The results indicated that the inoculated-drought-stressed plants maintained significantly higher values of all of the measured yield parameters, where the yielded grains had higher amounts of the direct reducing sugars, sucrose, starch; lower contents of total soluble proteins and the total free amino acids, and altered protein patterns compared to those of the uninoculated-drought-stressed plants. SDS-PAGE of the yielded grains showed that drought led to the appearance of some newly synthesized stress protein bands and disappearance of others. Inoculation with PGPB resulted in the re-appearance of some of the disappearing bands and the synthesis of new others. Meanwhile, wheat inoculation under normal conditions resulted in significantly promoted grain yields with higher contents of carbohydrates, total soluble proteins and total free amino acids than that of the uninoculated control. It has been proved that treating wheat plants with the PEG-tolerant Azospirillum brasilense NO40 or Stenotrophomonas maltophilia that were compatible with the systems into which they were introduced and possess multiple plant growth promoting traits, can be an efficient strategy to enhance wheat growth and productivity, not only under normal conditions, but also under drought stress.


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