scholarly journals Surface Charge-Dependent Cellular Uptake of Polystyrene Nanoparticles

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 1028 ◽  
Author(s):  
Soyeon Jeon ◽  
Jessica Clavadetscher ◽  
Dong-Keun Lee ◽  
Sunay Chankeshwara ◽  
Mark Bradley ◽  
...  

The evaluation of the role of physicochemical properties in the toxicity of nanoparticles is important for the understanding of toxicity mechanisms and for controlling the behavior of nanoparticles. The surface charge of nanoparticles is suggested as one of the key parameters which decide their biological impact. In this study, we synthesized fluorophore-conjugated polystyrene nanoparticles (F-PLNPs), with seven different types of surface functional groups that were all based on an identical core, to evaluate the role of surface charge in the cellular uptake of nanoparticles. Phagocytic differentiated THP-1 cells or non-phagocytic A549 cells were incubated with F-PLNP for 4 h, and their cellular uptake was quantified by fluorescence intensity and confocal microscopy. The amount of internalized F-PLNPs showed a good positive correlation with the zeta potential of F-PLNPs in both cell lines (Pearson’s r = 0.7021 and 0.7852 for zeta potential vs. cellular uptake in THP-1 cells and nonphagocytic A549 cells, respectively). This result implies that surface charge is the major parameter determining cellular uptake efficiency, although other factors such as aggregation/agglomeration, protein corona formation, and compositional elements can also influence the cellular uptake partly or indirectly.

Biology ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 307 ◽  
Author(s):  
Kornélia Bodó ◽  
Nicoló Baranzini ◽  
Rossana Girardello ◽  
Bohdana Kokhanyuk ◽  
Péter Németh ◽  
...  

Earthworms and leeches are sentinel animals that represent the annelid phylum within terrestrial and freshwater ecosystems, respectively. One early stress signal in these organisms is related to innate immunity, but how nanomaterials affect it is poorly characterized. In this survey, we compare the latest literature on earthworm and leeches with examples of their molecular/cellular responses to inorganic (silver nanoparticles) and organic (carbon nanotubes) nanomaterials. A special focus is placed on the role of annelid immunocytes in the evolutionarily conserved antioxidant and immune mechanisms and protein corona formation and probable endocytosis pathways involved in nanomaterial uptake. Our summary helps to realize why these environmental sentinels are beneficial to study the potential detrimental effects of nanomaterials.


2020 ◽  
Vol 8 (22) ◽  
pp. 4870-4882 ◽  
Author(s):  
Ana Peigneux ◽  
Emanuel A. Glitscher ◽  
Rawan Charbaji ◽  
Christoph Weise ◽  
Stefanie Wedepohl ◽  
...  

Colloidal stability and cellular uptake of MamC-biomimetic magnetite nanoparticles (BMNPs) incubated with human plasma (PC-BMNPs).


Nanoscale ◽  
2019 ◽  
Vol 11 (48) ◽  
pp. 23259-23267 ◽  
Author(s):  
Alberto Piloni ◽  
Chin Ken Wong ◽  
Fan Chen ◽  
Megan Lord ◽  
Andreas Walther ◽  
...  

Patterned nanoparticle surfaces can repel protein absorption and prevent the formation of a protein corona, which alters the biological behavior and therefore the fate of the nanoparticle.


2020 ◽  
Vol 21 (2) ◽  
pp. 519
Author(s):  
Idoia Mikelez-Alonso ◽  
Antonio Aires ◽  
Aitziber L. Cortajarena

Immunotherapy has become a promising cancer therapy, improving the prognosis of patients with many different types of cancer and offering the possibility for long-term cancer remission. Nevertheless, some patients do not respond to these treatments and immunotherapy has shown some limitations, such as immune system resistance or limited bioavailability of the drug. Therefore, new strategies that include the use of nanoparticles (NPs) are emerging to enhance the efficacy of immunotherapies. NPs present very different pharmacokinetic and pharmacodynamic properties compared with free drugs and enable the use of lower doses of immune-stimulating molecules, minimizing their side effects. However, NPs face issues concerning stability in physiological conditions, protein corona (PC) formation, and accumulation in the target tissue. PC formation changes the physicochemical and biological properties of the NPs and in consequence their therapeutic effect. This review summarizes the recent advances in the study of the effects of PC formation in NP-based immunotherapy. PC formation has complex effects on immunotherapy since it can diminish (“immune blinding”) or enhance the immune response in an uncontrolled manner (“immune reactivity”). Here, future perspectives of the field including the latest advances towards the use of personalized protein corona in cancer immunotherapy are also discussed.


2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Reza Fattah ◽  
Hamid Rashedi ◽  
Fatemeh Yazdian ◽  
Seyed Babak Mousavi ◽  
Ahmad Fazeli

Small ◽  
2017 ◽  
Vol 13 (16) ◽  
pp. 1603847 ◽  
Author(s):  
Alyssa B. Chinen ◽  
Chenxia M. Guan ◽  
Caroline H. Ko ◽  
Chad A. Mirkin

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