Protein corona formation and its influence on biomimetic magnetite nanoparticles

Patterned nanoparticle surfaces can repel protein absorption and prevent the formation of a protein corona, which alters the biological behavior and therefore the fate of the nanoparticle.

Download Full-text

Protein Corona Formation on Magnetite Nanoparticles: Effects of Culture Medium Composition, and Its Consequences on Superparamagnetic Nanoparticle Cytotoxicity

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2015.2000 ◽

2015 ◽

Vol 11 (5) ◽

pp. 828-840 ◽

Cited By ~ 9

Author(s):

D. A. Mbeh ◽

T. Javanbakht ◽

L. Tabet ◽

Y. Merhi ◽

K. Maghni ◽

...

Keyword(s):

Magnetite Nanoparticles ◽

Culture Medium ◽

Protein Corona ◽

Medium Composition ◽

Corona Formation

Download Full-text

Surface Charge-Dependent Cellular Uptake of Polystyrene Nanoparticles

Nanomaterials ◽

10.3390/nano8121028 ◽

2018 ◽

Vol 8 (12) ◽

pp. 1028 ◽

Cited By ~ 18

Author(s):

Soyeon Jeon ◽

Jessica Clavadetscher ◽

Dong-Keun Lee ◽

Sunay Chankeshwara ◽

Mark Bradley ◽

...

Keyword(s):

Zeta Potential ◽

Surface Charge ◽

Cellular Uptake ◽

A549 Cells ◽

Protein Corona ◽

Polystyrene Nanoparticles ◽

Biological Impact ◽

Corona Formation ◽

Different Types

The evaluation of the role of physicochemical properties in the toxicity of nanoparticles is important for the understanding of toxicity mechanisms and for controlling the behavior of nanoparticles. The surface charge of nanoparticles is suggested as one of the key parameters which decide their biological impact. In this study, we synthesized fluorophore-conjugated polystyrene nanoparticles (F-PLNPs), with seven different types of surface functional groups that were all based on an identical core, to evaluate the role of surface charge in the cellular uptake of nanoparticles. Phagocytic differentiated THP-1 cells or non-phagocytic A549 cells were incubated with F-PLNP for 4 h, and their cellular uptake was quantified by fluorescence intensity and confocal microscopy. The amount of internalized F-PLNPs showed a good positive correlation with the zeta potential of F-PLNPs in both cell lines (Pearson’s r = 0.7021 and 0.7852 for zeta potential vs. cellular uptake in THP-1 cells and nonphagocytic A549 cells, respectively). This result implies that surface charge is the major parameter determining cellular uptake efficiency, although other factors such as aggregation/agglomeration, protein corona formation, and compositional elements can also influence the cellular uptake partly or indirectly.

Download Full-text

The Impact of Protein Corona Formation on the Macrophage Cellular Uptake and Biodistribution of Spherical Nucleic Acids

Small ◽

10.1002/smll.201603847 ◽

2017 ◽

Vol 13 (16) ◽

pp. 1603847 ◽

Cited By ~ 37

Author(s):

Alyssa B. Chinen ◽

Chenxia M. Guan ◽

Caroline H. Ko ◽

Chad A. Mirkin

Keyword(s):

Nucleic Acids ◽

Cellular Uptake ◽

Protein Corona ◽

Corona Formation ◽

Spherical Nucleic Acids ◽

The Impact

Download Full-text

Protein source and choice of anticoagulant decisively affect nanoparticle protein corona and cellular uptake

Nanoscale ◽

10.1039/c5nr08196c ◽

2016 ◽

Vol 8 (10) ◽

pp. 5526-5536 ◽

Cited By ~ 80

Author(s):

S. Schöttler ◽

Katja Klein ◽

K. Landfester ◽

V. Mailänder

Keyword(s):

Human Plasma ◽

Cellular Uptake ◽

Protein Corona ◽

Protein Source ◽

Protein Sources ◽

P Protein

Protein corona investigations use different protein sources. Outcome of investigations is crucial. Human plasma seems to be a better choice than serum from animals or also human.

Download Full-text

Surface properties modulate protein corona formation and determine cellular uptake and cytotoxicity of silver nanoparticles

Nanoscale ◽

10.1039/d0nr08259g ◽

2021 ◽

Author(s):

Marianna Barbalinardo ◽

Jessika Bertacchini ◽

Linda Bergamini ◽

Maria Sara Magarò ◽

Luca Ortolani ◽

...

Keyword(s):

Silver Nanoparticles ◽

Surface Properties ◽

Cellular Uptake ◽

Biomedical Applications ◽

Protein Corona ◽

Diagnosis And Treatment ◽

Corona Formation ◽

Basic Understanding

Nanoparticles (NPs) have been studied for biomedical applications, ranging from prevention, diagnosis and treatment of diseases. However, the lack of the basic understanding of how NPs interact with the biological...

Download Full-text

Protein corona – from molecular adsorption to physiological complexity

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.6.88 ◽

2015 ◽

Vol 6 ◽

pp. 857-873 ◽

Cited By ~ 72

Author(s):

Lennart Treuel ◽

Dominic Docter ◽

Michael Maskos ◽

Roland H Stauber

Keyword(s):

Protein Interactions ◽

Colloidal Stability ◽

Protein Corona ◽

Recent Advances ◽

Corona Formation ◽

Protein Properties ◽

Coulomb Type ◽

First Time ◽

Physiological Complexity

In biological environments, nanoparticles are enshrouded by a layer of biomolecules, predominantly proteins, mediating its subsequent interactions with cells. Detecting this protein corona, understanding its formation with regards to nanoparticle (NP) and protein properties, and elucidating its biological implications were central aims of bio-related nano-research throughout the past years. Here, we discuss the mechanistic parameters that are involved in the protein corona formation and the consequences of this corona formation for both, the particle, and the protein. We review consequences of corona formation for colloidal stability and discuss the role of functional groups and NP surface functionalities in shaping NP–protein interactions. We also elaborate the recent advances demonstrating the strong involvement of Coulomb-type interactions between NPs and charged patches on the protein surface. Moreover, we discuss novel aspects related to the complexity of the protein corona forming under physiological conditions in full serum. Specifically, we address the relation between particle size and corona composition and the latest findings that help to shed light on temporal evolution of the full serum corona for the first time. Finally, we discuss the most recent advances regarding the molecular-scale mechanistic role of the protein corona in cellular uptake of NPs.

Download Full-text