scholarly journals Vitamin E Deficiency Disrupts Gene Expression Networks during Zebrafish Development

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 468
Author(s):  
Brian Head ◽  
Stephen A. Ramsey ◽  
Chrissa Kioussi ◽  
Robyn L. Tanguay ◽  
Maret G. Traber
Keyword(s):  
Gene Expression ◽  
Vitamin E ◽  
Energy Metabolism ◽  
Gene Transcription ◽  
Expression Profiling ◽  
Intracellular Signaling ◽  
Metabolic Dysfunction ◽  
Gene Set Enrichment ◽  
Vitamin E Deficiency ◽  
Transcriptional Dysregulation

Vitamin E (VitE) is essential for vertebrate embryogenesis, but the mechanisms involved remain unknown. To study embryonic development, we fed zebrafish adults (>55 days) either VitE sufficient (E+) or deficient (E–) diets for >80 days, then the fish were spawned to generate E+ and E– embryos. To evaluate the transcriptional basis of the metabolic and phenotypic outcomes, E+ and E– embryos at 12, 18 and 24 h post-fertilization (hpf) were subjected to gene expression profiling by RNASeq. Hierarchical clustering, over-representation analyses and gene set enrichment analyses were performed with differentially expressed genes. E– embryos experienced overall disruption to gene expression associated with gene transcription, carbohydrate and energy metabolism, intracellular signaling and the formation of embryonic structures. mTOR was apparently a major controller of these changes. Thus, embryonic VitE deficiency results in genetic and transcriptional dysregulation as early as 12 hpf, leading to metabolic dysfunction and ultimately lethal outcomes.

scholarly journals Chronic vitamin E deficiency impairs cognitive function in adult zebrafish via dysregulation of brain lipids and energy metabolism

2017 ◽  
Vol 112 ◽  
pp. 308-317 ◽  
Author(s):  
Melissa McDougall ◽  
Jaewoo Choi ◽  
Kathy Magnusson ◽  
Lisa Truong ◽  
Robert Tanguay ◽  
...  
Keyword(s):  
Vitamin E ◽  
Cognitive Function ◽  
Energy Metabolism ◽  
Adult Zebrafish ◽  
Vitamin E Deficiency ◽  
Brain Lipids

scholarly journals Gene Expression Profiling of Listeria monocytogenes Strain F2365 during Growth in Ultrahigh-Temperature-Processed Skim Milk

2008 ◽  
Vol 74 (22) ◽  
pp. 6859-6866 ◽  
Author(s):  
Yanhong Liu ◽  
Amy Ream
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
Listeria Monocytogenes ◽  
Energy Metabolism ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Binding Proteins ◽  
Skim Milk ◽  
Genes Encoding ◽  
Ultrahigh Temperature

ABSTRACT To study how Listeria monocytogenes survives and grows in ultrahigh-temperature-processed (UHT) skim milk, microarray technology was used to monitor the gene expression profiles of strain F2365 in UHT skim milk. Total RNA was isolated from strain F2365 in UHT skim milk after 24 h of growth at 4°C, labeled with fluorescent dyes, and hybridized to “custom-made” commercial oligonucleotide (35-mers) microarray chips containing the whole genome of L. monocytogenes strain F2365. Compared to L. monocytogenes grown in brain heart infusion (BHI) broth for 24 h at 4°C, 26 genes were upregulated (more-than-twofold increase) in UHT skim milk, whereas 14 genes were downregulated (less-than-twofold decrease). The upregulated genes included genes encoding transport and binding proteins, transcriptional regulators, proteins in amino acid biosynthesis and energy metabolism, protein synthesis, cell division, and hypothetical proteins. The downregulated genes included genes that encode transport and binding proteins, protein synthesis, cellular processes, cell envelope, energy metabolism, a transcriptional regulator, and an unknown protein. The gene expression changes determined by microarray assays were confirmed by real-time reverse transcriptase PCR analyses. Furthermore, cells grown in UHT skim milk displayed the same sensitivity to hydrogen peroxide as cells grown in BHI, demonstrating that the elevated levels of expression of genes encoding manganese transporter complexes in UHT skim milk did not result in changes in the oxidative stress sensitivity. To our knowledge, this report represents a novel study of global transcriptional gene expression profiling of L. monocytogenes in a liquid food.

Differential gene expression in skeletal muscle of rats with Vitamin E deficiency

IUBMB Life ◽  
2006 ◽  
Vol 58 (9) ◽  
pp. 540-548 ◽  
Author(s):  
Bettina Nier ◽  
Peter Weinberg ◽  
Gerald Rimbach ◽  
Elisabeth Stöcklin ◽  
Luca Barella
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Vitamin E ◽  
Differential Gene Expression ◽  
Vitamin E Deficiency ◽  
Differential Gene

Effect of Selenium and Vitamin E Deficiency on Differential Gene Expression in Rat Liver

2001 ◽  
Vol 285 (2) ◽  
pp. 470-475 ◽  
Author(s):  
Alexandra Fischer ◽  
Josef Pallauf ◽  
Kishorchandra Gohil ◽  
Stefan Udo Weber ◽  
Lester Packer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Vitamin E ◽  
Differential Gene Expression ◽  
Rat Liver ◽  
Vitamin E Deficiency ◽  
Differential Gene

Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio)

2021 ◽  
pp. 108801
Author(s):  
Alexander T. Watt ◽  
Brian Head ◽  
Scott W. Leonard ◽  
Robyn L. Tanguay ◽  
Maret G. Traber
Keyword(s):  
Gene Expression ◽  
Vitamin E ◽  
Danio Rerio ◽  
Vitamin E Deficiency

A temporal study of gene expression in rat lung following fixed-volume hemorrhage

2005 ◽  
Vol 23 (3) ◽  
pp. 275-286 ◽  
Author(s):  
P. D. Bowman ◽  
J. L. Sondeen ◽  
B. Zhao ◽  
V. G. Coppes ◽  
J. J. Nelson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Intracellular Signaling ◽  
Mononuclear Cells ◽  
Lung Damage ◽  
Pcr Analysis ◽  
Oligonucleotide Microarrays ◽  
Expression Of Genes ◽  
Temporal Study

Previous studies have indicated that hemorrhage may predispose the lung to respiratory distress syndrome. Gene expression profiling with oligonucleotide microarrays was used to evaluate the genetic responses of the lung to hemorrhage. Conscious rats, chronically instrumented with a catheter and telemetry device to record blood pressure, heart rate, and temperature, had 40% of their estimated blood volume removed at a rate of 1 ml/min over 7–10 min. Groups of three or more rats were euthanized at 1, 3, 6, 16, 24, 48, or 72 h following hemorrhage. Two additional groups were unmanipulated controls and instrumented animals with sham hemorrhage. Total RNA was isolated from lung, reverse-transcribed to cDNA, fluorescently labeled, and hybridized to oligonucleotide microarrays probing 5,671 rat genes. After hemorrhage, statistically detectable alteration of expression was seen in ∼0.8% of the genes at some time during the 72-h test period (vs. sham hemorrhage) as determined by false discovery rate statistics in the statistical analysis of microarrays program. A subset was confirmed by RT-PCR analysis. Hemorrhage influenced genes that regulate intracellular signaling and structure, growth factors, and hormonal receptors. There also appeared to be increased expression of genes that may mediate sequestration of neutrophils and mononuclear cells from the circulation. This hemorrhage model, although producing severe hemodynamic alterations, avoided mortality and histological evidence of lung damage, a feature intended to help ensure reliable evaluation of gene expression. These results indicate that gene expression profiling with microarrays provides a new tool for exploring the response of a tissue to systemic blood loss.

Vitamin E deficiency disrupts molecular pathways of energy metabolism in zebrafish embryos

2018 ◽  
Vol 128 ◽  
pp. S85
Author(s):  
Brian Head ◽  
Chrissa Kioussi ◽  
Scott W. Leonard ◽  
Robert Tanguay ◽  
Maret Traber
Keyword(s):  
Vitamin E ◽  
Energy Metabolism ◽  
Zebrafish Embryos ◽  
Molecular Pathways ◽  
Vitamin E Deficiency
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