scholarly journals Selenium Modulates the Allergic Response to Whey Protein in a Mouse Model for Cow’s Milk Allergy

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2479
Author(s):  
Xiaoli Zhao ◽  
Suzan Thijssen ◽  
Hongbing Chen ◽  
Johan Garssen ◽  
Leon M. J. Knippels ◽  
...  
Keyword(s):  
Mast Cell ◽  
Food Allergy ◽  
Whey Protein ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Control Group ◽  
Milk Allergy ◽  
Cow's Milk ◽  
Cow's Milk Allergy ◽  
Increased Risk

Cow’s milk allergy is a common food allergy in infants, and is associated with an increased risk of developing other allergic diseases. Dietary selenium (Se), one of the essential micronutrients for humans and animals, is an important bioelement which can influence both innate and adaptive immune responses. However, the effects of Se on food allergy are still largely unknown. In the current study it was investigated whether dietary Se supplementation can inhibit whey-induced food allergy in an animal research model. Three-week-old female C3H/HeOuJ mice were intragastrically sensitized with whey protein and cholera toxin and randomly assigned to receive a control, low, medium or high Se diet. Acute allergic symptoms, allergen specific immunoglobulin (Ig) E levels and mast cell degranulation were determined upon whey challenge. Body temperature was significantly higher in mice that received the medium Se diet 60 min after the oral challenge with whey compared to the positive control group, which is indicative of impaired anaphylaxis. This was accompanied by reductions in antigen-specific immunoglobulins and reduced levels of mouse mast cell protease-1 (mMCP-1). This study demonstrates that oral Se supplementation may modulate allergic responses to whey by decreasing specific antibody responses and mMCP-1 release.

Alteration of Allergen Fold of Bos d 5 into a Hypoallergenic Vaccine for Immunotherapy of Cow’s Milk Allergy

2021 ◽  
pp. 1-12
Author(s):  
Yaqiong Cui ◽  
Yang Yu ◽  
Shuxiang Lin ◽  
Li Xu ◽  
Liuxu Li ◽  
...  
Keyword(s):  
Food Allergy ◽  
T Cell ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Milk Allergy ◽  
T Cell Epitopes ◽  
Dot Blot ◽  
Allergen Specific Immunotherapy ◽  
Cow's Milk ◽  
Cow's Milk Allergy

<b><i>Background:</i></b> Cow’s milk allergy (CMA) is the most common IgE-mediated food allergy and Bos d 5 is the major allergen in cow’s milk proteins. More than 60% of the patients with CMA are sensitized to this protein. <b><i>Methods and Results:</i></b> A recombinant protein, encoded by a synthetic gene and consisting of reassembled Bos d 5 fragments, was expressed in <i>E. coli</i> strain BL21 (DE3) cells and purified to homogeneity. The B5M lacked relevant IgE-reactivity and allergenic activity compared with Bos d 5 in dot-blot and basophil activation assays. T-cell proliferation experiments demonstrated that B5M preserved the main T cell epitopes of Bos d 5. Immunization of rabbits with B5M induced protective IgG antibodies that blocked the binding of patients’ IgE antibodies to the wild-type allergen and inhibited the degranulation of basophils induced by Bos d 5. <b><i>Conclusion:</i></b> Thus, we developed a new strategy, which was based on rational molecular reassembly for allergen-specific immunotherapy (AIT) of CMA and food allergy.

scholarly journals Oral immunotherapy for IgE-mediated cow’s milk allergy in children: a systematic review and meta-analysis

2021 ◽  
Author(s):  
Lujing Tang ◽  
Yu Yu ◽  
Xiangyuan Pu ◽  
Jie Chen
Keyword(s):  
Meta Analysis ◽  
Oral Immunotherapy ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Control Group ◽  
Milk Allergy ◽  
Cow's Milk ◽  
Cow's Milk Allergy ◽  
Statistical Heterogeneity ◽  
Ige Mediated

Background: Cow’s milk allergy(CMA) is the most common allergy in infants that decreases the quality of life of patients and their families. Standard treatment for CMA is the strict avoidance of milk, new treatment strategies such as oral immunotherapy (OIT) have been sought for patients with CMA . We aimed to assess the clinical efficacy and safety of OIT in the treatment of children with IgE-mediated cow’s milk allergy (IMCMA). Methods: We searched all randomized controlled trials (RCTs) in which OIT is used to treat children with IMCMA from 5 international electronic databases. We estimated a pooled relative ratio (RR) for each outcome using a Mantel-Haenzel fixed-effect model if statistical heterogeneity was low. Results: Eleven studies were chosen for meta-analysis, including a total of 469 children (242 OIT, 227 control). 176 patients (72.7%) in the OIT were desensitized compared to 49 patients in the control group (RR 7.35, 95%CI 2.82-19.13, p<0.0001). The desensitization effect of OIT was particularly significant in children over 3 years old (RR 18.05, 95%CI 6.48-50.26, p<0.00001). Although adverse effects were common, they usually involved mild reactions, but epinephrine use was more common in the OIT group (RR 7.69, 95%CI 2.16-27.33, p<0.002). Conclusion: OIT can lead to desensitization in the majority of individuals with IMCMA, especially in patients over 3-years old. A major problem of OIT is the frequency of adverse events, although most are mild. OIT may be an alternative treatment in the future.

scholarly journals Principles of personalized diet therapy of cow’s milk allergy in children

2015 ◽  
Vol 12 (2) ◽  
pp. 47-51
Author(s):  
V A Reviykina ◽  
I A Larkova ◽  
E D Kuvshinova ◽  
M I Shavkina ◽  
M V Boitcov ◽  
...  
Keyword(s):  
Food Allergy ◽  
Diet Therapy ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Milk Allergy ◽  
Diverse Range ◽  
Cow's Milk ◽  
Cow's Milk Allergy ◽  
Medical Nutrition ◽  
Infants And Young Children

Cow’s milk allergy protein (CMPA) is the leading cause of food allergy in infants and young children. The personalized diet prescription, providing individual medical nutrition can be reliable guaranty of effective treatment for diverse range of CMPA symptoms. In spite of the fact that there are a lot of special infant formula in Russia, pediatricians and allergologists have difficulties in choosing appropriate diet. The suggested algorithm of individual option of extensively hydrolyzed proteinbased formula for CMPA patients will help doctors to optimize diet.

scholarly journals Food allergy and anaphylaxis – 2040. Nutritional status and dietary intake in children with cow’s milk allergy

2013 ◽  
Vol 6 ◽  
pp. P125
Author(s):  
Maria Golebiowska Wawrzyniak ◽  
Grazyna Rowicka ◽  
Malgorzata Strucinska ◽  
Katarzyna Markiewicz
Keyword(s):  
Food Allergy ◽  
Nutritional Status ◽  
Dietary Intake ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Milk Allergy ◽  
Cow's Milk ◽  
Cow's Milk Allergy

scholarly journals Assessment of Inflammatory Markers in Children with Cow’s Milk Allergy Treated with a Milk-Free Diet

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1057
Author(s):  
Jadwiga Ambroszkiewicz ◽  
Joanna Gajewska ◽  
Magdalena Chełchowska ◽  
Grażyna Rowicka
Keyword(s):  
Clinical Symptoms ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Control Group ◽  
Low Grade ◽  
Healthy Children ◽  
Milk Allergy ◽  
Cow's Milk ◽  
Cow's Milk Allergy ◽  
Tnf Α

Background: The aim of the study was to establish whether the use of a strict milk-free diet in children with cow’s milk allergy, resulting in the resolution of clinical symptoms of the disease, also extinguishes the inflammatory reaction induced by the allergy. Methods: We examined 64 children (aged 3–6 years) with a diagnosed cow’s milk allergy who had been treated with an elimination diet for at least six months and showed remission of the disease’s clinical symptoms as a result of the treatment. The control group consisted of 30 healthy children of the same age following an unrestricted age-appropriate diet. Concentrations of cytokines, calprotectin, and adipokines (leptin, resistin, chemerin, neutrophilic lipocalin associated with gelatinase—NGAL) were determined in the serum samples obtained from the studied children by immunoenzymatic assays. Results: Patients with CMA had significantly higher median values of serum IL-6, TNF-α, resistin, chemerin and NGAL in comparison to the healthy children (p < 0.05, p < 0.001, p < 0.05, p < 0.01, p < 0.001, respectively). Serum concentrations of IL-10, leptin, calprotectin and CRP as well as in WBC count were in the same range in both studied groups. We observed direct statistically significant correlations between levels of IL-10 and CRP (p = 0.005), IL-10 and WBC (p = 0.045), TNF-α and WBC (p = 0.038), calprotectin and WBC (p < 0.001), chemerin and CRP (p < 0.001) as well as between NGAL and WBC (p = 0.002) in children with CMA. Conclusion: The use of a strict milk-free diet by children with CMA, resulting in the resolution of clinical symptoms of the disease, does not seem to extinguish the inflammation induced by the allergy. The findings of this study—elevated IL-6, TNF-α, resistin, chemerin and NGAL levels in patients with CMA—suggest that these parameters seem to be involved in the generation of a low-grade proinflammatory environment observed in cow‘s milk allergy and could be used to monitor the effectiveness of treatment.

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