scholarly journals Characterization of a Dengue Virus Serotype 1 Isolated from a Patient in Ciudad Juarez, Mexico

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 872
Author(s):  
Pedro M. Palermo ◽  
Antonio de la Mora-Covarrubias ◽  
Jeanette Orbegozo ◽  
Jessica A. Plante ◽  
Kenneth S. Plante ◽  
...  
Keyword(s):  
Urban Communities ◽  
Ciudad Juarez ◽  
Nucleotide Sequencing ◽  
Surveillance Program ◽  
Envelope Gene ◽  
Virus Serotype ◽  
Border Communities ◽  
Serotype 1 ◽  
Southeast Texas

Dengue (DEN) is the most important human arboviral disease worldwide. Sporadic outbreaks of DEN have been reported since 1980 in urban communities located along the border in southeast Texas and northern Mexico. Other than the Rio Grande Valley region of TX, autochthonous transmission of DENV has not been reported from any other US border communities. As part of a surveillance program for arthropod-borne viruses in Ciudad Juarez, Mexico, during November 2015, a blood sample was obtained from a female patient who experienced an undifferentiated fever and arthralgia. The plasma of the sample was tested for virus in Vero-76 and C6/36 cells. DENV serotype 1 (DENV-1) was isolated in the C6/36 cells, and nucleotide sequencing of the envelope gene and full genome grouped the DENV-1 isolate in the Central America clade. The patient had not traveled outside of Ciudad Juarez, Mexico, thus suggesting DENV-1 infection was acquired in this community.

scholarly journals Complete Genome Sequence Analysis of an Imported Dengue Virus Serotype 1 Strain from Myanmar

2018 ◽  
Vol 6 (27) ◽  
Author(s):  
Zhaoping Zeng ◽  
Jiandong Shi ◽  
Xiaofang Guo ◽  
Ling Mo ◽  
Ningzhu Hu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genome Sequence Analysis ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Complete Genome Sequence Analysis ◽  
Dengue Virus Serotype 1

It has been determined that recent dengue virus epidemics in Yunnan, China, originated from Southeast Asian strains. Here, we report the first complete genome sequence and molecular characterization of the imported dengue virus serotype 1 strain YNPE1.

scholarly journals Molecular characterization of the viral structural protein genes of 2018 dengue virus serotype 1 epidemic in Yunnan, Southwest China

2020 ◽  
Author(s):  
Qingping Lan ◽  
Yun Shu ◽  
Linhao Li ◽  
Xiyun Shan ◽  
Dehong Ma ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Homologous Recombination ◽  
Molecular Characterization ◽  
Structure Prediction ◽  
Southwest China ◽  
Secondary Structure Prediction ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotype 1

Abstract Background There was a dengue virus serotype 1 (DENV-1) epidemic from October to December in 2018 in Xishuangbanna, Yunnan, southwest China, neighboring Myanmar, Laos and Vietnam. And the molecular characterization, evolution and potential source of DENV from Xishuangbanna were investigate. Methods C (capsid)/prM(premembrane)/E (envelope) genes of DNEV isolated from 87 serum samples obtained from local patients were amplified and sequenced, then evaluated using mutation, phylogenetic and homologous recombination analyses and secondary structure prediction. Results Phylogenetic analysis show that all the DENV strains from Xishuangbanna can grouped in two branch of DENV-1. Out of 52, 50 of isolates were grouped in branch one, and were most similar to Fujian (DQ193572) and Singapore 2016(MF314188) strains. The other two isolates have the closest relationship to Myanmar 2017 (MG679801) . When compared with DENV-1SS (standard strain), we found the amino acid residue of E155 was mutation from T to S,which may related to the virulence of virus and no obvious homologous recombination signals. Secondary structure prediction showed that some changes were found in the helical of MN123849 and MN123854 strains and few changes observed in disordered region. Conclusions This study revealed the molecular characterization of structural genes of xishuangbanna, 2018 epidemic strain, providing a reference for molecular epidemiology, infection, pathogenicity and vaccine development .

scholarly journals Zika Virus Surveillance at the Human–Animal Interface in West-Central Brazil, 2017–2018

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1164 ◽  
Author(s):  
Alex Pauvolid-Corrêa ◽  
Helver Gonçalves Dias ◽  
Laura Marina Siqueira Maia ◽  
Grasiela Porfírio ◽  
Thais Oliveira Morgado ◽  
...  
Keyword(s):  
Real Time ◽  
Zika Virus ◽  
Whole Blood ◽  
Nucleotide Sequencing ◽  
Human Populations ◽  
Envelope Gene ◽  
Rt Pcr ◽  
Central Brazil ◽  
West Central ◽  
Virus Serotype

Zika virus (ZIKV) was first discovered in 1947 in Uganda but was not considered a public health threat until 2007 when it found to be the source of epidemic activity in Asia. Epidemic activity spread to Brazil in 2014 and continued to spread throughout the tropical and subtropical regions of the Americas. Despite ZIKV being zoonotic in origin, information about transmission, or even exposure of non-human vertebrates and mosquitoes to ZIKV in the Americas, is lacking. Accordingly, from February 2017 to March 2018, we sought evidence of sylvatic ZIKV transmission by sampling whole blood from approximately 2000 domestic and wild vertebrates of over 100 species in West-Central Brazil within the active human ZIKV transmission area. In addition, we collected over 24,300 mosquitoes of at least 17 genera and 62 species. We screened whole blood samples and mosquito pools for ZIKV RNA using pan-flavivirus primers in a real-time reverse-transcription polymerase chain reaction (RT-PCR) in a SYBR Green platform. Positives were confirmed using ZIKV-specific envelope gene real-time RT-PCR and nucleotide sequencing. Of the 2068 vertebrates tested, none were ZIKV positive. Of the 23,315 non-engorged mosquitoes consolidated into 1503 pools tested, 22 (1.5%) with full data available showed some degree of homology to insect-specific flaviviruses. To identify previous exposure to ZIKV, 1498 plasma samples representing 62 species of domestic and sylvatic vertebrates were tested for ZIKV-neutralizing antibodies by plaque reduction neutralization test (PRNT90). From these, 23 (1.5%) of seven species were seropositive for ZIKV and negative for dengue virus serotype 2, yellow fever virus, and West Nile virus, suggesting potential monotypic reaction for ZIKV. Results presented here suggest no active transmission of ZIKV in non-human vertebrate populations or in alternative vector candidates, but suggest that vertebrates around human populations have indeed been exposed to ZIKV in West-Central Brazil.

scholarly journals Characterization of dengue virus serotype 1 in epidemics in Porto Velho, Rondônia, in 2001-2003

2007 ◽  
Vol 40 (3) ◽  
pp. 268-271 ◽  
Author(s):  
Deusilene Souza Vieira ◽  
Eduardo Rezende Honda ◽  
Soraya Santos Pereira ◽  
Glauciane da Silva Bifano ◽  
Mauro Shugiro Tada ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Enzymatic Digestion ◽  
Universal Primers ◽  
Laboratory Confirmation ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Ns1 Gene ◽  
Virus Isolates ◽  
Porto Velho

The first dengue fever epidemic in the State of Rondônia (western region of Brazil) was recorded in 1997, without laboratory confirmation. Following this, there was an epidemic in Manaus, in the neighboring State of Amazon, in 1998, in which DENV-1 and DENV-2 viruses were isolated from patients. In the present paper, the serotype characterization of the dengue virus isolated from patients with clinically suspected dengue in Porto Velho, Rondônia, between 2001 and 2003 is described. One hundred and fifty blood samples were collected between the first and fifth days of symptoms. Seventy samples of virus isolates were subjected to dengue identification by means of RT-PCR using universal primers for the NS1 gene of DENV, which amplifies a 419 bp fragment. The amplicons obtained were subjected to enzymatic digestion to characterize the viral serotypes. All the samples analyzed were DENV-1. A nucleotide sequence randomly selected from one amplicon, which was also DENV-1, presented 98% similarity to sequences from Southeast Asia that were obtained from GenBank.

scholarly journals Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Ana Cristina Pérez de Diego ◽  
Pedro José Sánchez-Cordón ◽  
Ana Isabel de las Heras ◽  
José Manuel Sánchez-Vizcaíno
Keyword(s):  
Immune Response ◽  
Antibody Response ◽  
B Lymphocytes ◽  
Bluetongue Virus ◽  
Clinical Signs ◽  
Regulatory Cells ◽  
Boost Vaccination ◽  
Virus Serotype ◽  
Serotype 1

The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4+, CD8+, and WC1+), CD25+regulatory cells, or CD14+monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14+monocytes, CD25+regulatory cells, and CD8+cytotoxic T lymphocytes.

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