scholarly journals Analysis of the Effects of Food Additives on Porphyromonas gingivalis

Pathogens ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 65
Author(s):  
Mai Shinohara ◽  
Miki Maetani ◽  
Chiharu Kitada ◽  
Yasuko Nishigami ◽  
Ayaka Yazawa ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Human Cell ◽  
Biofilm Formation ◽  
Octanoic Acid ◽  
Periodontal Diseases ◽  
Food Additives ◽  
The Elderly ◽  
Decanoic Acid ◽  
Human Cell Lines ◽  
Periodontopathogenic Bacteria

This study aims to investigate six food additives (octanoic acid, decanoic acid, acesulfame K, aspartame, saccharin, and sucralose) used in foods for the elderly or people with dysphagia because of the effect of these food additives on Porphyromonas gingivalis (P. gingivalis), which is a keystone pathogen of periodontal diseases. The growth of P. gingivalis was inhibited by 5 mM octanoic acid, 1.25 mM decanoic acid, 1.25% acesulfame K, 0.0625% aspartame, 0.03125% saccharin, and 0.625% sucralose. In addition, these food additives showed bactericidal activity for planktonic P. gingivalis (5 mM octanoic acid, 5 mM decanoic acid, 0.25% aspartame, 0.25% saccharin, and 5% sucralose). Moreover, biofilm formation was inhibited by 10 mM octanoic acid, 10 mM decanoic acid, 10% acesulfame K, 0.35% aspartame, 0.5% saccharin, and 7.5% sucralose. Moreover, the same concentration of these food additives without aspartame killed P. gingivalis in the biofilm. Aspartame and sucralose did not show cytotoxicity to human cell lines at concentrations that affected P. gingivalis. These findings may be useful in clarifying the effects of food additives on periodontopathogenic bacteria.

scholarly journals Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation of Porphyromonas gingivalis and Prevotella intermedia

2009 ◽  
Vol 53 (8) ◽  
pp. 3308-3316 ◽  
Author(s):  
Hiroyuki Wakabayashi ◽  
Koji Yamauchi ◽  
Tetsuo Kobayashi ◽  
Tomoko Yaeshima ◽  
Keiji Iwatsuki ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Periodontal Diseases ◽  
Antimicrobial Protein ◽  
Antibiofilm Activity ◽  
Prevotella Intermedia ◽  
Periodontopathic Bacteria ◽  
Oral Pathogens ◽  
In Vitro Effects

ABSTRACT Lactoferrin (LF) is an iron-binding antimicrobial protein present in saliva and gingival crevicular fluids, and it is possibly associated with host defense against oral pathogens, including periodontopathic bacteria. In the present study, we evaluated the in vitro effects of LF-related agents on the growth and biofilm formation of two periodontopathic bacteria, Porphyromonas gingivalis and Prevotella intermedia, which reside as biofilms in the subgingival plaque. The planktonic growth of P. gingivalis and P. intermedia was suppressed for up to 5 h by incubation with ≥130 μg/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and ≥6 μg/ml of bLF-derived antimicrobial peptide lactoferricin B (LFcin B); but those effects were weak after 8 h. The biofilm formation of P. gingivalis and P. intermedia over 24 h was effectively inhibited by lower concentrations (≥8 μg/ml) of various iron-bound forms (the apo, native, and holo forms) of bLF and hLF but not LFcin B. A preformed biofilm of P. gingivalis and P. intermedia was also reduced by incubation with various iron-bound bLFs, hLF, and LFcin B for 5 h. In an examination of the effectiveness of native bLF when it was used in combination with four antibiotics, it was found that treatment with ciprofloxacin, clarithromycin, and minocycline in combination with native bLF for 24 h reduced the amount of a preformed biofilm of P. gingivalis compared with the level of reduction achieved with each agent alone. These results demonstrate the antibiofilm activity of LF with lower iron dependency against P. gingivalis and P. intermedia and the potential usefulness of LF for the prevention and treatment of periodontal diseases and as adjunct therapy for periodontal diseases.

scholarly journals Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)

Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 412 ◽  
Author(s):  
Fátima Brandão ◽  
Natalia Fernández-Bertólez ◽  
Fernanda Rosário ◽  
Maria João Bessa ◽  
Sónia Fraga ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Culture Media ◽  
Cell Model ◽  
Titanium Dioxide Nanoparticles ◽  
Food Additives ◽  
Toxicity Assessment ◽  
Consumer Products ◽  
Human Cell Lines ◽  
Tio2 Nps

Titanium dioxide nanoparticles (TiO2 NPs) have a wide variety of applications in many consumer products, including as food additives, increasing the concern about the possible hazards that TiO2 NPs may pose to human health. Although most previous studies have focused on the respiratory system, ingestion must also be considered as an important exposure route. Furthermore, after inhalation or ingestion, TiO2 NPs can reach several organs, such as the liver, brain or lungs. Taking this into consideration, the present study focuses on the uptake and potential genotoxicity (micronuclei induction) of TiO2 NPs on four human cell lines of diverse origin: lung cells (A549), liver cells (HepG2), glial cells (A172) and neurons (SH-SY5Y), using flow cytometry methods. Results showed a concentration-, time- and cell-type- dependent increase in TiO2 NPs uptake but no significant induction of micronuclei in any of the tested conditions. Data obtained reinforce the importance of cell model and testing protocols choice for toxicity assessment. However, some questions remain to be answered, namely on the role of cell culture media components on the agglomeration state and mitigation of TiO2 NPs toxic effects.

scholarly journals Protein targets in the red complex organisms binding with an herbal compound silymarin

2020 ◽  
Vol 16 (10) ◽  
pp. 759-764
Author(s):  
Nadhirah Faiz ◽  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Periodontal Diseases ◽  
Treponema Denticola ◽  
Tannerella Forsythia ◽  
Protein Targets ◽  
Dental Biofilm ◽  
Optimal Drug ◽  
Herbal Compound

Periodontitis is attributed to the dental biofilm formation caused by various microbial changes that occurs in the biofilm. Red complex organisms are a group of organisms linked with periodontal diseases. Therefore, it is of interest to identify potential targets from the red complex organisms to bind with the herbal compound silymarin. We report a list of potential proteins having optimal drug like binding features with the herbal agent silymarin for further consideration. We used the STITCH v.5 pipeline using VICMPred and VirulentPred tools to identify such targets as potential virulent factors in the red complex organisms. We considered the strains of Porphyromonas gingivalis ATCC 33277, Treponema denticola ATCC 35405 and Tannerella forsythia ATCC 43037 in the red complex pathogens for this analysis. Protein targets in the red complex organisms with optimal binding features with the herbal compound silymarin were thus identified and reported for further consideration.

scholarly journals Identification of protein targets in red complex organisms binding with resveratrol

2020 ◽  
Vol 16 (11) ◽  
pp. 837-842
Author(s):  
Keshaav Krishnaa Pothapur ◽  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Periodontal Diseases ◽  
Treponema Denticola ◽  
Tannerella Forsythia ◽  
Protein Targets ◽  
Dental Biofilm ◽  
Optimal Drug ◽  
Herbal Compound

Periodontitis is attributed to the dental biofilm formation caused by various Red complex organisms are a group of organisms linked with periodontal diseases. Therefore, it is of interest to identify potential targets from the red complex organisms to bind with the herbal compound resveratrol (E - 5 - (4 - hydroxy styryl) benzene 1,3 diol). We report a list of potential proteins having optimal drug like binding features with the herbal agent Resveratrol for further consideration. We used the STITCH v.5 pipeline using VICMPred and VirulentPred tools to identify such targets as potential virulent factors in the red complex organisms. We considered the strains of Porphyromonas gingivalis ATCC 33277, Treponema denticola ATCC 35405 and Tannerella forsythia ATCC 43037 in the red complex pathogens for this analysis. Protein targets in the red complex organisms with optimal binding features with the herbal compound resveratrol were thus identified and reported for further consideration.

Investigations of the influence of microgravity on the state of human cell lines

2004 ◽  
Vol 10 (5-6) ◽  
pp. 226-228
Author(s):  
L.M. Nosach ◽  
◽  
O.Yu. Povnitsa ◽  
V.L. Zhovnovata ◽  
◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
The State ◽  
Human Cell Lines

Complex treatment of periodontitis: patient’s reaction to the therapy

2020 ◽  
Vol 25 (2) ◽  
pp. 97-100
Author(s):  
A. K. Iordanishvili ◽  
V. A. Guk ◽  
A. A. Golovko
Keyword(s):  
Elderly People ◽  
Periodontal Diseases ◽  
Personal Characteristics ◽  
The Elderly ◽  
Comprehensive Treatment ◽  
Complex Treatment ◽  
Middle Aged ◽  
Aged People ◽  
Dental Rehabilitation ◽  
Success Of Treatment

Relevance. The success of treatment of periodontal diseases directly depends on the patient’s response to the therapy, therefore, the characteristics of the person’s personal characteristics can affect both the effectiveness of treatment and the prevention of relapse of the disease.Purpose. To study the features of the internal picture of the disease in the process of complex treatment of adult patients suffering from chronic generalized periodontitis.Materials and methods. The generally accepted comprehensive treatment of chronic generalized periodontitis in 69 middle-aged and elderly men was carried out taking into account the personal response of patients Solovyov «Psychosensory-anatomical-functional maladaptation syndrome».Results. When patients were discharged from the hospital, there was a difference in the phenomena of maladaptation among the elderly and middle-aged: in middle-aged people, sufficient adaptation to the conditions of existence was determined; in elderly people, due to the existing comorbid pathology, a state of maladaptation was diagnosed, which was caused by the presence of complaints of defects in the dentition.Conclusion. In elderly people, as soon as possible after completion of treatment in a hospital, dental rehabilitation cannot be considered completed, which requires the adoption of organizational measures for their timely provision of dentures.

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