scholarly journals Concentrations of Acute-Phase Proteins in Milk from Cows with Clinical Mastitis Caused by Different Pathogens

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 706
Author(s):  
Felipe M. Dalanezi ◽  
Elizabeth M. S. Schmidt ◽  
Sâmea F. Joaquim ◽  
Felipe F. Guimarães ◽  
Simoni T. Guerra ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Diagnostic Methods ◽  
Spatial Proximity ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A ◽  
Positive Correlations

Among the new diagnostic methods for mastitis detection under development, milk acute-phase proteins (APPs) are receiving special attention. The study aimed to compare the profile of milk APPs from cows with natural clinical mastitis caused by distinct pathogens. The concentrations of haptoglobin (Hp), serum amyloid A (SAA), alpha-1-acid glycoprotein (AGP), and C-reactive protein (CRP) were measured by Spatial Proximity Analyte Reagent Capture Luminescence (SPARCL). Each APP was compared across the pathogens causing mastitis. The APPs differed statistically (p < 0.05) among the pathogens causing udder infection. There were significant and positive correlations among the concentration profile, for each pathogen, in three of four APPs studied. It can be concluded that the pathogen causing mastitis could modify the profile of release of the APPs in milk. The profile of Hp, AGP, and CRP demonstrated significant correlation, indicating that the three APPs are suggested as biomarkers, in milk, for bovine mastitis.

Variations in the levels of acute-phase proteins and lactoferrin in serum and milk during bovine subclinical mastitis

2021 ◽  
pp. 1-5
Author(s):  
Aarsha Raj ◽  
Vinodkumar Kulangara ◽  
Tresamol P. Vareed ◽  
Deepa P. Melepat ◽  
Latha Chattothayil ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Solid Phase ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Reactive Protein ◽  
Amyloid A ◽  
California Mastitis Test ◽  
Bacterial Organism

Abstract Variations in the levels of acute phase proteins and lactoferrin in serum and milk for diagnosis of subclinical mastitis in dairy cows are described in this research paper. Milking animals from two organized dairy farms in Kerala, India, were screened by California Mastitis Test (CMT), Electrical Conductivity test (EC) and Somatic Cell Count (SCC) test to identify animals affected with sub clinical mastitis (SCM). The concentrations of acute phase proteins (APP) Haptoglobin (Hp), C- reactive protein (CRP), Albumin, Lactoferrin (Lf) and α- 1 acid glycoprotein (AGP) in milk and Hp, Albumin, Serum Amyloid A (SAA) and CRP in the serum of 40 normal cows and 40 cows affected with sub clinical mastitis were assessed. Solid phase ELISA was employed for assessment of all parameters except the albumin levels, for which spectrophotometry was used. The values of Hp in milk; and SAA, AGP and Lf in serum, were significantly elevated in the group with sub clinical mastitis. Such variations were found to be independent of the specific bacterial organism causing the disease. These results show that significant variations exist in the levels of acute phase proteins Hp, AGP and Lf in milk, and SAA in serum of animals affected with subclinical bovine mastitis that are not affected by specific bacterial etiology.

The acute phase response and C-reactive protein

2020 ◽  
pp. 2199-2207
Author(s):  
Mark B. Pepys
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Phase Response ◽  
Serum Amyloid ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A ◽  
Serum Amyloid A Protein

The acute phase response—trauma, tissue necrosis, infection, inflammation, and malignant neoplasia induce a complex series of nonspecific systemic, physiological, and metabolic responses including fever, leucocytosis, catabolism of muscle proteins, greatly increased de novo synthesis and secretion of a number of ‘acute phase’ plasma proteins, and decreased synthesis of albumin, transthyretin, and high- and low-density lipoproteins. The altered plasma protein concentration profile is called the acute phase response. Acute phase proteins—these are mostly synthesized by hepatocytes, in which transcription is controlled by cytokines including interleukin 1, interleukin 6, and tumour necrosis factor. The circulating concentrations of complement proteins and clotting factors increase by up to 50 to 100%; some of the proteinase inhibitors and α‎1-acid glycoprotein can increase three- to fivefold; but C-reactive protein (CRP) and serum amyloid A protein (an apolipoprotein of high-density lipoprotein particles) are unique in that their concentrations can change by more than 1000-fold. C-reactive protein—this consists of five identical, nonglycosylated, noncovalently associated polypeptide subunits. It binds to autologous and extrinsic materials which contain phosphocholine, including bacteria and their products. Ligand-bound CRP activates the classical complement pathway and triggers the inflammatory and opsonizing activities of the complement system, thereby contributing to innate host resistance to pneumococci and probably to recognition and safe ‘scavenging’ of cellular debris. Clinical features—(1) determination of CRP in serum or plasma is the most useful marker of the acute phase response in most inflammatory and tissue damaging conditions. (2) Acute phase proteins may be harmful in some circumstances. Sustained increased production of serum amyloid A protein can lead to the deposition of AA-type, reactive systemic amyloid.

Abstract B87: Acute-phase proteins (C-reactive protein and Serum Amyloid A) and post-diagnosis mammographic density in breast cancer survivors

2012 ◽  
Vol 5 (11 Supplement) ◽  
pp. B87-B87
Author(s):  
Anne Dee ◽  
Roberta McKean-Cowdin ◽  
Anne McTiernan ◽  
Richard N. Baumgartner ◽  
Kathy B. Baumgartner ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Survivors ◽  
Mammographic Density ◽  
Acute Phase ◽  
Breast Cancer Survivors ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A

scholarly journals The Acute-Phase Proteins Serum Amyloid A and C Reactive Protein in Transudates and Exudates

2006 ◽  
Vol 2006 ◽  
pp. 1-6 ◽  
Author(s):  
Alessandra M. Okino ◽  
Cristiani Bürger ◽  
Jefferson R. Cardoso ◽  
Edson L. Lavado ◽  
Paulo A. Lotufo ◽  
...  
Keyword(s):  
Acute Phase ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Serum Amyloid ◽  
Strong Positive Correlation ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A ◽  
Protein Serum ◽  
Serum Ratio

The distinction between exudates and transudates is very important in the patient management. Here we evaluate whether the acute-phase protein serum amyloid A (SAA), in comparison with C reactive protein (CRP) and total protein (TP), can be useful in this discrimination. CRP, SAA, and TP were determined in 36 exudate samples (27 pleural and 9 ascitic) and in 12 transudates (9 pleural and 3 ascitic). CRP, SAA, and TP were measured. SAA present in the exudate corresponded to10%of the amount found in serum, that is, the exudate/serum ratio (E/S) was0.10±0.13. For comparison, the exudate/serum ratio for CRP and TP was0.39±0.37and0.68±0.15, respectively. There was a strong positive correlation between serum and exudate SAA concentration (r=0.764;p<0.0001). The concentration of SAA in transudates was low and did not overlap with that found in exudates (0.02-0.21 versus 0.8–360.5 g/mL). SAA in pleural and ascitic exudates results mainly from leakage of the serum protein via the inflamed membrane. A comparison of the E/S ratio of SAA and CRP points SAA as a very good marker in discriminating between exudates and transudates

scholarly journals Changes in Acute-Phase Proteins in Plasma during the Periparturient Period of Dairy Goats

2021 ◽  
Vol 8 (12) ◽  
pp. 311
Author(s):  
Fangyuan Zeng ◽  
Bingyu Shen ◽  
Yang Yuan ◽  
Yezi Kong ◽  
Panpan Tan ◽  
...  
Keyword(s):  
Acute Phase ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
C Reactive Protein ◽  
Dairy Goats ◽  
Upward Trend ◽  
Blood Calcium ◽  
Reactive Protein ◽  
Amyloid A ◽  
Periparturient Period

The present study was conducted regarding four acute-phase proteins (APPs) including C-reactive protein (CRP), ceruloplasmin (CP), serum amyloid A (SAA), and haptoglobin (HP) in dairy goats during the periparturient period. The aim of this study was to detect the changes in APPs in plasma during the periparturient period of healthy dairy goats. Guanzhong dairy goats with no other symptoms (n = 15) were selected on the basis of their blood calcium (Ca) and β-hydroxybutyrate (BHBA) concentration. The plasma was collected once a week for ±3 weeks delivery. The concentrations of the four APPs mentioned above were determined using goat-specific ELISA kits. The results showed the CRP level in plasma decreased from 3 weeks to 1 week antepartum and increased later until 1 week postpartum and then decreased to a similar level with antepartum between 1 and 3 weeks postpartum. The content of CP showed a decline in 3 weeks before parturition and an upward trend between 1 week antepartum and 3 weeks postpartum. The SAA concentration decreased from 3 weeks antepartum to 2 weeks postpartum and rebounded later. The level of HP decreased during 3 weeks before parturition and increased until 1 week postpartum, then reached a stable value. Clear variation range and rules of APPs contribute to perinatal health monitoring of dairy goats.

scholarly journals Acute Phase Proteins as Markers of Infectious Diseases in small Animals / Proteini Akutne Faze Kao Markeri Infektivnih Bolesti Malih Životinja

2015 ◽  
Vol 65 (2) ◽  
pp. 149-161 ◽  
Author(s):  
Elizabeth Moreira dos Santos Schmidt ◽  
Peter David Eckersall
Keyword(s):  
Infectious Diseases ◽  
Acute Phase ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Current Knowledge ◽  
Small Animals ◽  
C Reactive Protein ◽  
Peripheral Tissues ◽  
Reactive Protein ◽  
Amyloid A

Abstract During the acute phase response, there is an increased production and release of certain proteins known as acute phase proteins (APPs) which can be produced by hepatocytes and peripheral tissues such as C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp), alpha-1 acid glycoprotein (AGP). These proteins have been investigated as markers of various infectious diseases in small animals and the purpose of this review is to update the current knowledge about APPs in infectious diseases in dogs and cats.

scholarly journals Major Acute Phase Proteins in Pig Serum from Birth to Slaughter

2012 ◽  
Vol 56 (4) ◽  
pp. 553-557 ◽  
Author(s):  
Małgorzata Pomorska-Mól ◽  
Krzysztof Kwit ◽  
Iwona Markowska-Daniel
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Clinical Importance ◽  
Diagnostic Value ◽  
Individual Variability ◽  
Reactive Protein ◽  
Amyloid A ◽  
Age Related ◽  
Age Dependent ◽  
Positive Correlations

Abstract Age-related changes in serum concentrations of C-reactive protein (CRP), haptoglobin (Hp), serum amyloid A (SAA), and pig major acute phase protein (pig-MAP) were investigated in healthy pigs from birth to slaughter under field conditions. Repeated blood samples were obtained from 60 pigs at ages of 1-19 weeks. Concentrations of acute phase proteins (APP) were measured with the use of commercial ELISA kits. Concentrations of all APP increased with age (P<0.05) and positive correlations were evidenced between their concentrations and the age of pigs. Great variations in CRP, Hp, and SAA concentrations were found, as can been seen from standard deviation values. The minimal individual variability was found in regard to pig-MAP. A significant increase in all APP was observed in pigs’ serum after weaning, constituting an important characteristic of this period. The elevation of APP after weaning may be associated with stress induced by mixing animals after weaning or changes in the pattern of feed administration. The peak in APP may be also caused by the initiation of synthesis of these proteins by piglets. Because a significant association between age and APP concentrations exists, further studies are needed to decide whether the age may influence the diagnostic value of APP as a marker of infection. Additionally, studies are needed to estimate whether the APP response in infection is age-dependent to any clinical importance degree.

scholarly journals Acute phase proteins, C-reactive protein and serum amyloid A protein, as prognostic markers in the elderly inpatient

1997 ◽  
Vol 26 (2) ◽  
pp. 153-158 ◽  
Author(s):  
MAXINE B. HOGARTH ◽  
J. RUTH GALLIMORE ◽  
PHILIP SAVAGE ◽  
ALISON J. PALMER ◽  
JOHN M. STARR ◽  
...  
Keyword(s):  
Acute Phase ◽  
Serum Amyloid A ◽  
Prognostic Markers ◽  
Acute Phase Proteins ◽  
The Elderly ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A ◽  
Serum Amyloid A Protein ◽  
Elderly Inpatient
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