scholarly journals Sustained-Release and pH-Adjusted Alginate Microspheres-Encapsulated Doxorubicin Inhibit the Viabilities in Hepatocellular Carcinoma-Derived Cells

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1417
Author(s):  
Cheng-Tang Pan ◽  
Ruei-Siang Yu ◽  
Chih-Jung Yang ◽  
Lih-Ren Chen ◽  
Zhi-Hong Wen ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Sustained Release ◽  
Flow Rate ◽  
Calcium Alginate ◽  
Ultrasonic Atomization ◽  
Median Diameter ◽  
Cytotoxicity Effects ◽  
Micrometer Scale ◽  
Dose Dependent

The objective of this study aimed to develop biodegradable calcium alginate microspheres carrying doxorubicin (Dox) at the micrometer-scale for sustained release and the capacity of pH regulatory for transarterial chemoembolization. Ultrasonic atomization and CaCl2 cross-linking technologies were used to prepare the microspheres. A 4-by-5 experiment was first designed to identify imperative parameters. The concentration of CaCl2 and the flow rate of the pump were found to be critical to generate microspheres with a constant volume median diameter (~39 μm) across five groups with different alginate: NaHCO3 ratios using each corresponding flow rate. In each group, the encapsulation efficiency was positively correlated to the Dox-loading %. Fourier-transform infrared spectroscopy showed that NaHCO3 and Dox were step-by-step incorporated into the calcium alginate microspheres successfully. Microspheres containing alginate: NaHCO3 = 1 exhibited rough and porous surfaces, high Young’s modulus, and hardness. In each group with the same alginate: NaHCO3 ratio, the swelling rates of microspheres were higher in PBS containing 10% FBS compared to those in PBS alone. Microspheres with relatively high NaHCO3 concentrations in PBS containing 10% FBS maintained better physiological pH and higher accumulated Dox release ratios. In two distinct hepatocellular carcinoma-derived cell lines, treatments with microspheres carrying Dox demonstrated that the cell viabilities decreased in groups with relatively high NaHCO3 ratios in time- and dose-dependent manners. Our results suggested that biodegradable alginate microspheres containing relatively high NaHCO3 concentrations improved the cytotoxicity effects in vitro.

scholarly journals Sustained-Release and pH-Adjusted Alginate Microspheres-Encapsulated Doxorubicin Inhibit the Viabilities in Hepatocellular Carcinoma-Derived Cells

2021 ◽  
Author(s):  
Cheng-Tang Pan ◽  
Ruei-Siang Yu ◽  
Chih-Jung Yang ◽  
Lih-Ren Chen ◽  
Zhi-Hong Wen ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Sustained Release ◽  
Flow Rate ◽  
Calcium Alginate ◽  
Ultrasonic Atomization ◽  
Median Diameter ◽  
Cytotoxicity Effects ◽  
Micrometer Scale ◽  
Dose Dependent

The objective of this study aimed to develop biodegradable calcium alginate microspheres carrying doxorubicin (Dox) at the micrometer-scale for sustained-release and the capacity of pH regulatory for transarterial chemoembolization. Ultrasonic atomization and CaCl2 cross-linking technologies were used to prepare the microspheres. A 4 by 5 experiment was first designed to identify imperative parameters. The concentration of CaCl2 and the flow rate of the pump were found to be critical to generate microspheres with a constant volume median diameter (~ 39 m) across 5 groups with different alginate:NaHCO3 ratios using each corresponding flow rate. In each group, the encapsulation efficiency was positively correlated to the Dox-loaded efficiency. Fourier-transform infrared spectroscopy showed that NaHCO3 and Dox were step-by-step incorporated into the calcium alginate microspheres successfully. Microspheres containing alginate:NaHCO3 = 1 exhibited rough and porous surfaces, high Young’s modulus and hardness. In each group with the same alginate:NaHCO3 ratio, the swelling rates of microspheres were higher in PBS containing 10% FBS compared to those in PBS alone. Microspheres with relative high NaHCO3 concentrations in PBS containing 10% FBS maintained better physiological pH and higher accumulated Dox release ratios. In two distinct hepatocellular carcinoma-derived cell lines, treatments with microspheres carrying Dox demonstrated that the cell viabilities decreased in groups with relative high NaHCO3 ratios in time- and dose-dependent manners. Our results suggested that biodegradable alginate microspheres containing relative high NaHCO3 concentrations improved the cytotoxicity effects in vitro.

Chemoprevention Against Hepatocellular Carcinoma of Cornus officinalis in vitro

2004 ◽  
Vol 32 (05) ◽  
pp. 717-725 ◽  
Author(s):  
Jung-San Chang ◽  
Lien-Chai Chiang ◽  
Fen-Fang Hsu ◽  
Chun-Ching Lin
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Lines ◽  
Leukemic Cell ◽  
Leukemic Cells ◽  
Xtt Assay ◽  
Leukemic Cell Lines ◽  
Cornus Officinalis ◽  
Anti Oxidant ◽  
Dose Dependent

The water extracts of Cornus officinalis Sieb. et Zuce against hepatocellular carcinoma (HCC) was studied for its chemopreventive potential. Three HCC cell lines (HepG2, SK-Hep1 and PLC/PRF/5) and three leukemic cell lines (U937, K562 and Raji) were tested with XTT assay. Extracts of C. officinalis inhibited all these HCC cells and leukemic cells at a concentration of 100 μg/ml (P<0.05) and was dose-dependent (P<0.0001). P53 (P<0.0001) and Ras (P=0.001) significantly affected its activity against HCC. Extracts of C. officinalis also possessed the anti-oxidant activity through free radicals scavenging activity at a concentration of 50 μg/ml (P<0.05). In summary, our experiment implied that C. officinalis might be a candidate for chemopreventive agent against HCC through the antioxidant and anti-neoplastic effects.

scholarly journals Metformin decreases hepatocellular carcinoma risk in a dose-dependent manner: population-based and in vitro studies

Gut ◽  
2012 ◽  
Vol 62 (4) ◽  
pp. 606-615 ◽  
Author(s):  
Hsiao-Ping Chen ◽  
Jeng-Jer Shieh ◽  
Chia-Che Chang ◽  
Tzu-Ting Chen ◽  
Jaw-Town Lin ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Population Based ◽  
In Vitro Studies ◽  
Dependent Manner ◽  
Dose Dependent ◽  
Carcinoma Risk

scholarly journals Evaluation of Tetrandrine Sustained Release Calcium Alginate Gel Beads In Vitro and In Vivo

2009 ◽  
Vol 129 (7) ◽  
pp. 851-854 ◽  
Author(s):  
Yan MA ◽  
Wei-Zhong LI ◽  
Shi-Xia GUAN ◽  
Xiao-Ping LAI ◽  
Da-Wei CHEN
Keyword(s):  
Sustained Release ◽  
Calcium Alginate ◽  
Alginate Gel ◽  
Gel Beads ◽  
Calcium Alginate Gel

scholarly journals Chitosan-Alginate Nanoparticle System Efficiently Delivers Doxorubicin to MCF-7 Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Nuwanthi P. Katuwavila ◽  
A. D. L. Chandani Perera ◽  
Sameera R. Samarakoon ◽  
Preethi Soysa ◽  
Veranja Karunaratne ◽  
...  
Keyword(s):  
Sustained Release ◽  
Slow Release ◽  
Sodium Tripolyphosphate ◽  
Chitosan Nanoparticles ◽  
Time Dependency ◽  
Initial Release ◽  
Nanoparticle System ◽  
Dose Dependent ◽  
Mcf 7

A chitosan-alginate nanoparticle system encapsulating doxorubicin (DOX) was prepared by a novel ionic gelation method using alginate as the crosslinker. These nanoparticles were around 100 nm in size and more stable with higher positive zeta potential and had higher % encapsulation efficiency (95%) than DOX loaded chitosan nanoparticles (DOX Csn NP) crosslinked with sodium tripolyphosphate (STPP). FTIR spectroscopy and thermogravimetric analysis revealed successful loading of DOX.In vitrodrug release showed an initial release phase followed by slow release phase with higher cumulative release obtained with DOX loaded chitosan-alginate nanoparticles (DOX Csn-Alg NP). Thein vitrocytotoxicity of DOX released from the two nanoparticle systems showed a notable difference on comparison with that of free DOX on the MCF-7 cell line. The SRB assay, AO/EB staining, and fluorescence uptake study indicated that free DOX only showed dose dependent cytotoxicity, whereas both dose and time dependency were exhibited by the two sets of NPs. While both systems show sustained release of DOX, from the cell viability plots, DOX Csn-Alg NPs showed their superiority over DOX Csn NPs. The results obtained are useful for developing DOX Csn-Alg NPs as a sustained release carrier system for DOX.

scholarly journals 596 Metformin Decreases Hepatocellular Carcinoma Risk in a Dose-Dependent Manner: Population-Based and In Vitro Studies

2012 ◽  
Vol 142 (5) ◽  
pp. S-920 ◽  
Author(s):  
Chun-Ying Wu ◽  
Hsiao-Ping Chen ◽  
Tzu-Ting Chen ◽  
Jaw-Town Lin ◽  
Ming-Shiang Wu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Population Based ◽  
In Vitro Studies ◽  
Dependent Manner ◽  
Dose Dependent ◽  
Carcinoma Risk

scholarly journals In Vitro Cytotoxicity Test Reveals Non-toxic of Waste-based Scaffold on Human Hepatocyte Cells

2021 ◽  
Vol 10 (1) ◽  
pp. 109
Author(s):  
Oktantia Frenny Anggani ◽  
Aniek Setiya Budiatin ◽  
Laksmi Sulmartiwi ◽  
Muhammad Rahmad Royan
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Viability ◽  
Chondroitin Sulfate ◽  
In Vitro Cytotoxicity ◽  
Human Hepatocyte ◽  
Cytotoxicity Test ◽  
C 50 ◽  
Hepatocyte Cells ◽  
Dose Dependent

Scaffold, as one of the components for bone tissue engineering, requires formulated biomaterials that are both structurally and compositively similar to bone composition. Among others, chitosan, gelatin and chondroitin sulfate are known as potential candidates for scaffold composites that can be easily obtained from waste-based resources. This study aims to investigate the cytotoxicity of different scaffold composition and concentration regimes derived from waste-based chitosan, gelatine and chondroitin sulfate, in vitro. The composition regimes used were (Chitosan : Gelatin : Chondroitin Sulfate) 50 : 50 : 0 (A); 50 : 40 :10 (B); 50 : 35 : 15 (C); 50 : 30 : 20 (D); 50 : 25 : 25 (E). Meanwhile, the final concentrations of scaffold used were 2000, 1000, 500, 250, 100, 10 and 0,1 mg/ml. The different compositions and concentrations of scaffold was tested against Hepatocellular Carcinoma (Huh7it / Human Hepatocyte It). After 48-hour incubation in the scaffold solution, the percentage of cell viability was evaluated using 3-(4,5-dimethylthiazol-2yl)-5(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium (MTT) assay. The result shows that there is no difference observed among different scaffold compositions on the cell viability (p > 0.05). However, different concentrations of scaffold show significant differences in cell viability in composition C and E (p < 0.01), suggesting possible dose- dependent effect of scaffold on cell viability. Overall, all the waste-based scaffold compositions show no toxicity against the Hepatocellular Carcinoma cells as exhibited by the cell viability that is above 70%, at least with the concentration up to 2000 mg/ml.

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