scholarly journals Microfluidic Production of Polymeric Core-Shell Microspheres for the Delayed Pulsatile Release of Bovine Serum Albumin as a Model Antigen

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1854
Author(s):  
Renée S. van der Kooij ◽  
Rob Steendam ◽  
Johan Zuidema ◽  
Henderik W. Frijlink ◽  
Wouter L. J. Hinrichs

For many vaccines, multiple injections are required to confer protective immunity against targeted pathogens. These injections often consist of a primer administration followed by a booster administration of the vaccine a few weeks or months later. A single-injection vaccine formulation that provides for both administrations could greatly improve the convenience and vaccinee’s compliance. In this study, we developed parenterally injectable core-shell microspheres with a delayed pulsatile release profile that could serve as the booster in such a vaccine formulation. These microspheres contained bovine serum albumin (BSA) as the model antigen and poly(dl-lactide-co-glycolide) (PLGA) with various dl-lactide:glycolide monomer ratios as the shell material. Highly monodisperse particles with different particle characteristics were obtained using a microfluidic setup. All formulations exhibited a pulsatile in vitro release of BSA after an adjustable lag time. This lag time increased with the increasing lactide content of the polymer and ranged from 3 to 7 weeks. Shell thickness and bovine serum albumin loading had no effect on the release behavior, which could be ascribed to the degradation mechanism of the polymer, with bulk degradation being the main pathway. Co-injection of the core-shell microspheres together with a solution of the antigen that serves as the primer would allow for the desired biphasic release profile. Altogether, these findings show that injectable core-shell microspheres combined with a primer are a promising alternative for the current multiple-injection vaccines.

2010 ◽  
Vol 215 (1) ◽  
pp. 118-122 ◽  
Author(s):  
Milohum Mikesokpo Dzagli ◽  
Valentin Canpean ◽  
Monica Iosin ◽  
Messanh Agbeko Mohou ◽  
Simion Astilean

2018 ◽  
Vol 47 (30) ◽  
pp. 10223-10228 ◽  
Author(s):  
Zuozhong Liang ◽  
Zhiyuan Yang ◽  
Haitao Yuan ◽  
Chun Wang ◽  
Jing Qi ◽  
...  

We have synthesized a core@shell nanocomposite using biocompatible bovine serum albumin (BSA) as the core and a pH-sensitive metal–organic framework (MOF) as the shell.


2019 ◽  
Author(s):  
Francesco Carraro ◽  
Miriam Velasquez ◽  
Efwita Astria ◽  
Weibin Liang ◽  
liam Twight ◽  
...  

Here we systematically varied the composition of the relative amounts of ligand (2-methylimidazole), metal precursor (Zn(OAc)2∙2H2O, and protein to prepare a series of protein@ZIF biocomposites. The effect of post synthetic treatments (i.e. washes with water or water/ethanol) was investigated. The XRD data of the examined samples were used to construct ternary diagrams. Five different phases were identified. The encapsulation efficiency (of bovine serum albumin and insulin) were phase dependent.


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