scholarly journals Mapping of Genetic Locus for Leaf Trichome Formation in Chinese Cabbage Based on Bulked Segregant Analysis

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 771
Author(s):  
Rujia Zhang ◽  
Yiming Ren ◽  
Huiyuan Wu ◽  
Yu Yang ◽  
Mengguo Yuan ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Chinese Cabbage ◽  
Bulked Segregant Analysis ◽  
Genetic Locus ◽  
Serine Threonine Kinase ◽  
Trichome Formation ◽  
Family Protein ◽  
Leaf Trichome ◽  
Atp Binding Protein

Chinese cabbage is a leafy vegetable, and its leaves are the main edible organs. The formation of trichomes on the leaves can significantly affect its taste, so studying this phenomenon is of great significance for improving the quality of Chinese cabbage. In this study, two varieties of Chinese cabbage, W30 with trichome leaves and 082 with glabrous leaves, were crossed to generate F1 and F1 plants, which were self-fertilized to develop segregating populations with trichome or glabrous morphotypes. The two bulks of the different segregating populations were used to conduct bulked segregant analysis (BSA). A total of 293.4 M clean reads were generated from the samples, and plants from the trichome leaves (AL) bulk and glabrous leaves (GL) bulk were identified. Between the two DNA pools generated from the trichome and glabrous plants, 55,048 SNPs and 272 indels were generated. In this study, three regions (on chromosomes 6, 10 and scaffold000100) were identified, and the annotation revealed three candidate genes that may participate in the formation of leaf trichomes. These findings suggest that the three genes—Bra025087 encoding a cyclin family protein, Bra035000 encoding an ATP-binding protein/kinase/protein kinase/protein serine/threonine kinase and Bra033370 encoding a WD-40 repeat family protein–influence the formation of trichomes by participating in trichome morphogenesis (GO: 0010090). These results demonstrate that BSA can be used to map genes associated with traits and provide new insights into the molecular mechanism of leafy trichome formation in Chinese cabbage.

scholarly journals cot1: A Regulator of Arabidopsis Trichome Initiation

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 565-577
Author(s):  
Daniel B Szymanski ◽  
Daniel A Klis ◽  
John C Larkin ◽  
M David Marks
Keyword(s):  
Cell Fate ◽  
Gene Dosage ◽  
Signal Transduction Pathway ◽  
Spatial Arrangement ◽  
Complex Signal ◽  
Chromosome 2 ◽  
Wild Type ◽  
Trichome Formation ◽  
In The Wild ◽  
Leaf Trichome

Abstract In Arabidopsis, the timing and spatial arrangement of trichome initiation is tightly regulated and requires the activity of the GLABROUS1 (GL1) gene. The COTYLEDON TRICHOME 1 (COT1) gene affects trichome initiation during late stages of leaf development and is described in this article. In the wild-type background, cot1 has no observable effect on trichome initiation. GL1 overexpression in wild-type plants leads to a modest number of ectopic trichomes and to a decrease in trichome number on the adaxial leaf surface. The cot1 mutation enhances GL1-overexpression-dependent ectopic trichome formation and also induces increased leaf trichome initiation. The expressivity of the cot1 phenotype is sensitive to cot1 and 35S::GL1 gene dosage, and the most severe phenotypes are observed when cot1 and 35S::GL1 are homozygous. The COT1 locus is located on chromosome 2 15.3 cM north of er. Analysis of the interaction between cot1, try, and 35S::GL1 suggests that COT1 is part of a complex signal transduction pathway that regulates GL1-dependent adoption of the trichome cell fate.

Pre-slaughter transport, AMP-activated protein kinase, glycolysis, and quality of pork loin

Meat Science ◽  
2006 ◽  
Vol 74 (2) ◽  
pp. 388-395 ◽  
Author(s):  
Q.W. Shen ◽  
W.J. Means ◽  
S.A. Thompson ◽  
K.R. Underwood ◽  
M.J. Zhu ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Pork Loin ◽  
Amp Activated Protein Kinase

scholarly journals Role for Akt3/Protein Kinase Bγ in Attainment of Normal Brain Size

2005 ◽  
Vol 25 (5) ◽  
pp. 1869-1878 ◽  
Author(s):  
Rachael M. Easton ◽  
Han Cho ◽  
Kristin Roovers ◽  
Diana W. Shineman ◽  
Moshe Mizrahi ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Brain Size ◽  
Mammalian Target Of Rapamycin ◽  
Normal Growth ◽  
Cell Number ◽  
Normal Brain ◽  
Phosphatidylinositol 3 Kinase ◽  
Serine Threonine Kinase ◽  
Specific Regulation ◽  
Proportional Decrease

ABSTRACT Studies of Drosophila and mammals have revealed the importance of insulin signaling through phosphatidylinositol 3-kinase and the serine/threonine kinase Akt/protein kinase B for the regulation of cell, organ, and organismal growth. In mammals, three highly conserved proteins, Akt1, Akt2, and Akt3, comprise the Akt family, of which the first two are required for normal growth and metabolism, respectively. Here we address the function of Akt3. Like Akt1, Akt3 is not required for the maintenance of normal carbohydrate metabolism but is essential for the attainment of normal organ size. However, in contrast to Akt1 − / − mice, which display a proportional decrease in the sizes of all organs, Akt3 −/− mice present a selective 20% decrease in brain size. Moreover, although Akt1- and Akt3-deficient brains are reduced in size to approximately the same degree, the absence of Akt1 leads to a reduction in cell number, whereas the lack of Akt3 results in smaller and fewer cells. Finally, mammalian target of rapamycin signaling is attenuated in the brains of Akt3 −/− but not Akt1 −/− mice, suggesting that differential regulation of this pathway contributes to an isoform-specific regulation of cell growth.

scholarly journals Bulked Segregant Analysis Coupled With Whole-Genome Sequencing (BSA-Seq) and Identification of a Novel Locus, qGL3.5, That Regulates Grain Length

2021 ◽  
Author(s):  
Haiyan Zhao ◽  
Yuebin Zheng ◽  
Feng Bai ◽  
Yang Liu ◽  
Sicheng Deng ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Bulked Segregant Analysis ◽  
Functional Characterization ◽  
Quality Data ◽  
Rice Grain ◽  
Grain Length ◽  
Starting Point ◽  
New Gene ◽  
Membrane Bound

Abstract Rice grain length (GL) directly affects the yield and quality of this species. Very few GL-related genes cloned are applied in production because their yield-increasing effect was not obvious. In this study, the two bulk-DNA pools (L-pool and S-pool) and their parents’ (KJ01 and Huaye 4) DNAs were subjected to high-throughput sequencing. After assessing the quality of the data, we obtained a total of 100.22 Gb of high-quality data; the average coverage depth was 55x, and the genome coverage was 96.51%. After combining the association results of the ED and SNP index methods, we mapped the GL genes to a 0.34 Mb “hotspot” region on chromosome 3, which contains 37 genes related to various traits. The 37 predicted genes were further analyzed by the use of the Gene GO, COG database and so on. Thirty-three genes were annotated by GO functions. According to the GO annotations, three genes whose molecular function involved in the plasma membrane and intracellular membrane-bound organelles were detected via CRISPR/ Cas9 editing technology. ORF33 was verified to regulate GL and was the target gene qGL3.5. These results provides a new gene resource for rice grain shape breeding and a starting point for functional characterization of the wild rice GL gene.

Promotion of the Growth and Quality of Chinese Cabbage by Application of Biogas Slurry of Water Hyacinth

2012 ◽  
Vol 195-196 ◽  
pp. 1200-1206 ◽  
Author(s):  
Yan Feng Xue ◽  
Zhi Qi Shi ◽  
Jian Chen ◽  
Shao Hua Yan ◽  
Jian Chu Zheng
Keyword(s):  
Chinese Cabbage ◽  
Water Hyacinth ◽  
Quality Index ◽  
Soluble Sugar ◽  
Dependent Manner ◽  
Biogas Slurry ◽  
Fresh Weight ◽  
Shoot Height ◽  
Nitrite Content

Biomass and quality index of Chinese cabbage were investigated under the following treatment, control (CK), 25% biogas slurry + 75% fertilizer (Z-25%), 50% biogas slurry + 50% fertilizer (Z-50%), 75% biogas slurry + 25% fertilizer (Z-75%), and 100% biogas slurry (Z-100%). The results indicated that Z-25% significantly increased shoot height and fresh weight. But shoot height and fresh weight significantly decreased in a time-dependent manner when the concentration of the biogas slurry was greater than 50%. Vitamin C, soluble sugar, protein, and nitrite were measured to evaluate the effect of biogas slurry on the quality of Chinese cabbage. Z-25% treatment could remarkably increased the content of dehydroascorbate (DHA) and total Vc, whereas did not significantly affect reduced ascorbate (AsA) content compared to the CK for 60 d. The content soluble sugar and protein were significantly enhanced under Z-25% treatment. In addition, Z-25% effectively reduced nitrite content in Chinese cabbage. Biogas slurry with concentration more than 50% could not promote quality index of Chinese cabbage. To sum up, biogas slurry of water hyacinth could improve the growth and quality of Chinese cabbage under the optimal applied concentration.

scholarly journals Protein kinase CK2: structure, regulation and role in cellular decisions of life and death

2003 ◽  
Vol 369 (1) ◽  
pp. 1-15 ◽  
Author(s):  
David W. LITCHFIELD
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Current Knowledge ◽  
Cellular Functions ◽  
Serine Threonine Kinase ◽  
Life And Death ◽  
Dual Specificity ◽  
Mechanistic Basis ◽  
Kinase Ck2

Protein kinase CK2 ('casein kinase II') has traditionally been classified as a messenger-independent protein serine/threonine kinase that is typically found in tetrameric complexes consisting of two catalytic (α and/or α′) subunits and two regulatory β subunits. Accumulated biochemical and genetic evidence indicates that CK2 has a vast array of candidate physiological targets and participates in a complex series of cellular functions, including the maintenance of cell viability. This review summarizes current knowledge of the structural and enzymic features of CK2, and discusses advances that challenge traditional views of this enzyme. For example, the recent demonstrations that individual CK2 subunits exist outside tetrameric complexes and that CK2 displays dual-specificity kinase activity raises new prospects for the precise elucidation of its regulation and cellular functions. This review also discusses a number of the mechanisms that contribute to the regulation of CK2 in cells, and will highlight emerging insights into the role of CK2 in cellular decisions of life and death. In this latter respect, recent evidence suggests that CK2 can exert an anti-apoptotic role by protecting regulatory proteins from caspase-mediated degradation. The mechanistic basis of the observation that CK2 is essential for viability may reside in part in this ability to protect cellular proteins from caspase action. Furthermore, this anti-apoptotic function of CK2 may contribute to its ability to participate in transformation and tumorigenesis.

A visible light-controllable Rho kinase inhibitor based on a photochromic phenylazothiazole

2021 ◽  
Author(s):  
Kazuya Matsuo ◽  
Sampreeth Thayyil ◽  
Mitsuyasu Kawaguchi ◽  
Hidehiko Nakagawa ◽  
Nobuyuki Tamaoki
Keyword(s):  
Protein Kinase ◽  
Visible Light ◽  
Kinase Inhibitor ◽  
Coiled Coil ◽  
Rho Kinase ◽  
Rock Inhibitor ◽  
Serine Threonine Kinase ◽  
Threonine Kinase ◽  
Rho Kinase Inhibitor

Rho-associated coiled-coil-containing protein kinase (ROCK) is a serine-threonine kinase, whose inhibitors are useful for the regulation of actomyosin system. Here, we developed a photoswitchable ROCK inhibitor based on a phenylazothiazole...

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