scholarly journals Inheritance of Black Rot Resistance and Development of Molecular Marker Linked to Xcc Races 6 and 7 Resistance in Cabbage

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1940
Author(s):  
Jeong-Eui Hong ◽  
Khandker Shazia Afrin ◽  
Md Abdur Rahim ◽  
Hee-Jeong Jung ◽  
Ill-Sup Nou
Keyword(s):  
Molecular Marker ◽  
Xanthomonas Campestris ◽  
Black Rot ◽  
Recessive Trait ◽  
R Gene ◽  
Nucleotide Polymorphisms ◽  
F2 Population ◽  
Yield And Quality ◽  
Xanthomonas Campestris Pv Campestris

Black rot, caused by Xanthomonas campestris pv. campestris (Xcc), produces V-shaped chlorotic lesions on the leaves of cabbage (Brassica oleracea var. capitata L.), causing darkened veins and drastically reducing yield and quality. Of the 11 Xcc races identified, races 1, 4, and 6 are predominant globally. In the present study, we aimed to develop a molecular marker linked to black rot resistance against Xcc races 6 and 7. Crossed between black rot-resistant (‘SCNU-C-3470’) and -susceptible (‘SCNU-C-3328’) lines obtained 186 F2 plants. Resistance to Xcc race 6 segregated in a 3:1 (susceptible:resistant) ratio in the F2 population, which is consistent with a monogenic recessive trait. Nucleotide-binding site (NBS) leucine rich repeat (LRR)-encoding resistance (R) genes play a crucial role in plant defenses to various pathogens. The candidate R gene (Bol031422) located on chromosome C08, previously reported by our research group, was cloned and sequenced in resistant and susceptible cabbage lines. The R gene Bol031422 consisted of a single exon with a 3 bp insertion/deletions (InDels), a 292 bp polymorphism (an insertion in the exon of the resistant line relative to the susceptible line) and several single nucleotide polymorphisms (SNPs). Here, we developed the InDel marker BR6-InDel to assess linkage between variation at Bol031422 and resistance to Xcc races 6 and 7. This marker will help cabbage breeders develop cabbage cultivars resistant to Xcc races 6 and 7.

Improved control of black rot of broccoli caused by Xanthomonas campestris pv. campestris using a bacteriophage and a nonpathogenic Xanthomonas sp. strain

2017 ◽  
Vol 83 (6) ◽  
pp. 373-381 ◽  
Author(s):  
Hirofumi Nagai ◽  
Noriyuki Miyake ◽  
Shinro Kato ◽  
Daisuke Maekawa ◽  
Yasuhiro Inoue ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Black Rot ◽  
Xanthomonas Campestris Pv Campestris

scholarly journals Antagonism of yeasts to Xanthomonas campestris pv. campestris on cabbage phylloplane in field

1999 ◽  
Vol 30 (3) ◽  
pp. 191-195 ◽  
Author(s):  
Sayonara M.P. Assis ◽  
Rosa L.R. Mariano ◽  
Sami J. Michereff ◽  
Gil Silva ◽  
Elizabeth A.A. Maranhão
Keyword(s):  
Disease Severity ◽  
Xanthomonas Campestris ◽  
Antagonistic Activity ◽  
Black Rot ◽  
Significant Difference ◽  
Xanthomonas Campestris Pv Campestris

Twenty yeast isolates, obtained from cabbage phylloplane, were evaluated for antagonistic activity against Xanthomonas campestris pv. campestris, in field. Plants of cabbage cv. Midori were pulverized simultaneously with suspensions of antagonists and pathogen. After 10 days, plants were evaluated through percentage of foliar area with lesions. Percentage of disease severity reduction (DSR%) was also calculated. Yeast isolates LR32, LR42 and LR19 showed, respectively, 72, 75 and 79% of DSR. These antagonists were tested in seven different application periods in relation to pathogen inoculation (T1=4 d before; T2=simultaneously; T3=4 d after; T4=4 d before + simultaneously; T5=4 d after + simultaneously; T6=4 d before + 4 d after; T7=4 d before + simultaneously + 4 d after). The highest DSRs were showed by LR42 (71%), LR42 (67%), LR35 (69%) and LR19 (68%) in the treatments T7, T4, T5 and T6, which significantly differed from the others. The same yeast antagonists were also tested for black rot control using different cabbage cultivars (Fuyutoyo, Master-325, Matsukaze, Midori, Sekai I and Red Winner). The DSRs varied from 58 to 61%, and there was no significant difference among cultivars.

scholarly journals Identification of Black Rot Resistance in a Wild Brassica Species and Its Potential Transferability to Cauliflower

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1400
Author(s):  
Xiao-Guang Sheng ◽  
Ferdinando Branca ◽  
Zhen-Qing Zhao ◽  
Jian-Sheng Wang ◽  
Hui-Fang Yu ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Resistance Breeding ◽  
Brassica Species ◽  
Gene Location ◽  
F1 Hybrids ◽  
Black Rot ◽  
Resistant Parent ◽  
F2 Population ◽  
Related Wild Species ◽  
Wild Accessions

Black rot is a destructive disease that affects B. oleracea crops, causing significant losses to growers throughout the world. The purpose of this study was to screen out new sources resistant to Xanthomonas campestris pv. campestris race 4 (Xcc4) in 26 cauliflower and six related wild species, and to assess the inheritance of resistance. The results indicate that most of the tested accessions were susceptible or had intermediate resistance, except the Boc4601 (a cauliflower stable inbred line) and PI435896, UNICT5168, and UNICT5169 (wild accessions). Among them, UNICT5169 (Brassica montana) and PI435896 (Brassica balearica) showed the strongest resistance to Xcc4, with significantly lower disease index (DI), area of the infected part (AIP) and proportion of the infected part to the total leaf area (PTL) values. UNICT 5169 was selected as an Xcc4-resistant parent because of its relatively good cross seed-setting rate with cauliflower cultivars. F1 hybrids were successfully produced between this wild resistant accession (UNICT 5169) and one susceptible cauliflower breeding line (Boc3202-4), indicating the potential transferability of this resistance to cauliflower. The results of the symptoms severity evaluation of the F2 population indicate that Xcc4 resistance in UNICT5169 is a quantitative trait, which guides future resistance gene location and black rot resistance breeding.

scholarly journals Characterization of Xanthomonas campestris pv. campestris in Algeria

2021 ◽  
Vol 60 (1) ◽  
pp. 51-62
Author(s):  
Samia LAALA ◽  
Sophie CESBRON ◽  
Mohamed KERKOUD ◽  
Franco VALENTINI ◽  
Zouaoui BOUZNAD ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Housekeeping Genes ◽  
First Record ◽  
Black Rot ◽  
Physiological And Biochemical Characteristics ◽  
Main Production ◽  
Production Areas ◽  
Cruciferous Plants ◽  
Xanthomonas Campestris Pv Campestris

Xanthomonas campestris pv. campestris (Xcc) causes the black rot of cruciferous plants. This seed-borne bacterium is considered as the most destructive disease to cruciferous crops. Although sources of contamination are various, seeds are the main source of transmission. Typical symptoms of black rot were first observed in 2011 on cabbage and cauliflower fields in the main production areas of Algeria. Leaf samples displaying typical symptoms were collected during 2011 to 2014, and 170 strains were isolated from 45 commercial fields. Xcc isolates were very homogeneous in morphological, physiological and biochemical characteristics similar to reference strains, and gave positive pathogenicity and molecular test results (multiplex PCR with specific primers). This is the first record of Xcc in Algeria. Genetic diversity within the isolates was assessed in comparison with strains isolated elsewhere. A multilocus sequence analysis based on two housekeeping genes (gyrB and rpoD) was carried out on 77 strains representative isolates. The isolates grouped into 20 haplotypes defined with 68 polymorphic sites. The phylogenetic tree obtained showed that Xcc is in two groups, and all Algerian strains clustered in group 1 in three subgroups. No relationships were detected between haplotypes and the origins of the seed lots, the varieties of host cabbage, the years of isolation and agroclimatic regions.

Involvement of a novel peroxidase isozyme and lignification in hydathodes in resistance to black rot disease in cabbage

2000 ◽  
Vol 78 (9) ◽  
pp. 1144-1149 ◽  
Author(s):  
P A Gay ◽  
S Tuzun
Keyword(s):  
Xanthomonas Campestris ◽  
Virulent Strain ◽  
Black Rot ◽  
Peroxidase Isozyme ◽  
Anionic Peroxidase ◽  
Peroxidase Isozymes ◽  
Resistant Varieties ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris ◽  
Lignin Deposition

The physiological mechanisms associated with resistance of cabbage to black rot disease seem to be associated with the hydathodes. To investigate the role of hydathodes in disease resistance, total peroxidase activities, anionic peroxidase isozyme expression, and lignin deposition were determined in hydathodes of resistant (Hancock and Green Cup), partially resistant (Cheers), and susceptible (Strukton and Perfect Ball) cabbage varieties (Brassica oleracea L.) during pathogenesis with Xanthomonas campestris pv. campestris. Four-week-old plants were petiole-inoculated with a highly virulent strain of X. campestris pv. campestris (FD91L). Hydathodal fluids were collected daily over a 14-day period from infected plants as well as noninfected, mock-inoculated control plants. Hydathodal fluids of resistant varieties had greater peroxidase activity when compared to susceptible ones, with infected plants having higher peroxidase levels than noninfected plants. Isoelectric focusing revealed the presence of four anionic peroxidase isozymes in hydathodal fluids, with the most anionic one (pI of 3.6) accumulating only upon infection. Lignin deposition in and around the hydathodes was associated with the accumulation of this particular isozyme in hydathodal fluids. The evidence suggests that a rapid, systemic response is associated with resistance to the bacterial pathogen.Key words: peroxidases, hydathodes, isozymes, black rot disease, cabbage, Xanthomonas campestris pv. campestris.

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