Morphological Characterization and DNA Barcoding of Duckweed Species in Saudi Arabia

Duckweeds, or Lemnaceae, are widespread aquatic plants. Morphology-based identification of duckweed species is difficult because of their structural complexity. Hence, molecular tools provide significant advantages for characterizing and selecting species or clones for sustainable commercial use. In this study, we collected and characterized ten duckweed isolates from nine different regions in Saudi Arabia (SA). Based on the morphological characterization and phylogenetic analysis of intergenic spacer sequences of chloroplast DNA using six barcoding markers, the clones were classified into three genera, represented by seven species: Lemna gibba L., Lemna minor L., Lemna japonica Landolt, Lemna aequinoctialis Welw., Lemna perpusilla Torr., Spirodela polyryiza (L.) Schleid., and Landoltia punctate G. Mey. Lemna gibba was revealed to be a distinct dominant duckweed species in many regions of SA. Five barcoding markers showed that L. gibba, L. minor, and L. punctata were the most widely distributed species in the country. However, L. punctata, L. perpusilla, and S. polyryiza were the dominant species in the Al-Qassim, Madinah-1, and Madinah-2 regions, respectively. Moreover, the morphological traits revealed variations for these clones, relative to other studied duckweed clones. According to the results obtained in this study, three out of six plastid markers (trnH-psbA, matK, and atpF-atpH) helped to identify the dominant duckweed species in Saudi Arabia. Further evaluation based on adaptability, molecular genetic studies, and functional genomics is needed for these species to be used at the commercial level in Saudi Arabia.

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Molecular genetic studies of canine inherited diseases

10.32469/10355/14514 ◽

2011 ◽

Author(s):

Fabiana Helena Geraldo Farias

Keyword(s):

Molecular Genetic ◽

Genetic Studies ◽

Inherited Diseases

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From the clinic to the research laboratory. The role of the clinician in molecular genetic studies

Archives of Dermatology ◽

10.1001/archderm.129.11.1424 ◽

1993 ◽

Vol 129 (11) ◽

pp. 1424-1429

Author(s):

S. E. Palmer

Keyword(s):

Research Laboratory ◽

Molecular Genetic ◽

Genetic Studies

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Glucokinase gene structure. Functional implications of molecular genetic studies

Diabetes ◽

10.2337/diabetes.39.5.523 ◽

1990 ◽

Vol 39 (5) ◽

pp. 523-527 ◽

Cited By ~ 21

Author(s):

M. A. Magnuson

Keyword(s):

Gene Structure ◽

Molecular Genetic ◽

Glucokinase Gene ◽

Genetic Studies ◽

Functional Implications

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Molecular Genetic Studies of Bone Mechanical Strain and of Pedigrees with Very High Bone Density

10.21236/ada469247 ◽

2006 ◽

Author(s):

Subburaman Mohan

Keyword(s):

Bone Density ◽

Molecular Genetic ◽

Mechanical Strain ◽

Genetic Studies ◽

High Bone ◽

High Bone Density ◽

Very High

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Molecular Genetic Studies of Bone Mechanical Strain and of Pedigrees With Very High Bone Density

10.21236/ada538239 ◽

2008 ◽

Author(s):

Subburaman Mohan

Keyword(s):

Bone Density ◽

Molecular Genetic ◽

Mechanical Strain ◽

Genetic Studies ◽

High Bone ◽

High Bone Density ◽

Very High

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Molecular Genetic Studies of Bone Mechanical Strain and of Pedigrees with Very High Bone Density

10.21236/ada535566 ◽

2010 ◽

Author(s):

Subburaman Mohan

Keyword(s):

Bone Density ◽

Molecular Genetic ◽

Mechanical Strain ◽

Genetic Studies ◽

High Bone ◽

High Bone Density ◽

Very High

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Prospects for the Personalized Multimodal Therapy Approach to Pain Management via Action on NO and NOS

Molecules ◽

10.3390/molecules26092431 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2431

Author(s):

Natalia A. Shnayder ◽

Marina M. Petrova ◽

Tatiana E. Popova ◽

Tatiana K. Davidova ◽

Olga P. Bobrova ◽

...

Keyword(s):

Chronic Pain ◽

Molecular Genetic ◽

Pain Syndrome ◽

Medical Problem ◽

Single Nucleotide Variants ◽

Genetic Studies ◽

Pain Syndromes ◽

Nos Inhibitors ◽

Peripheral Pain

Chronic pain syndromes are an important medical problem generated by various molecular, genetic, and pathophysiologic mechanisms. Back pain, neuropathic pain, and posttraumatic pain are the most important pathological processes associated with chronic pain in adults. Standard approaches to the treatment of them do not solve the problem of pain chronicity. This is the reason for the search for new personalized strategies for the prevention and treatment of chronic pain. The nitric oxide (NO) system can play one of the key roles in the development of peripheral pain and its chronicity. The purpose of the study is to review publications devoted to changes in the NO system in patients with peripheral chronical pain syndromes. We have carried out a search for the articles published in e-Library, PubMed, Oxford Press, Clinical Case, Springer, Elsevier, and Google Scholar databases. The search was carried out using keywords and their combinations. The role of NO and NO synthases (NOS) isoforms in peripheral pain development and chronicity was demonstrated primarily from animal models to humans. The most studied is the neuronal NOS (nNOS). The role of inducible NOS (iNOS) and endothelial NOS (eNOS) is still under investigation. Associative genetic studies have shown that single nucleotide variants (SNVs) of NOS1, NOS2, and NOS3 genes encoding nNOS, iNOS, and eNOS may be associated with acute and chronic peripheral pain. Prospects for the use of NOS inhibitors to modulate the effect of drugs used to treat peripheral pain syndrome are discussed. Associative genetic studies of SNVs NOS1, NOS2, and NOS3 genes are important for understanding genetic predictors of peripheral pain chronicity and development of new personalized pharmacotherapy strategies.

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101 New variant prion protein in a Japanese family with Gerstmann-Sträussler syndrome : Clinicopathological , molecular genetic studies

Neurobiology of Aging ◽

10.1016/s0197-4580(96)80103-5 ◽

1996 ◽

Vol 17 (4) ◽

pp. S25-S26

Author(s):

H. Furukawa ◽

H. Tashiro ◽

Y. Tanaka ◽

C. Yutani ◽

T. Yamaguchi ◽

...

Keyword(s):

Prion Protein ◽

Molecular Genetic ◽

Japanese Family ◽

Genetic Studies ◽

New Variant

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Flavonoids in seeds and grains: physiological function, agronomic importance and the genetics of biosynthesis

Seed Science Research ◽

10.1017/s0960258500004372 ◽

1998 ◽

Vol 8 (4) ◽

pp. 415-422 ◽

Cited By ~ 97

Author(s):

Brenda Winkel Shirley

Keyword(s):

Secondary Metabolites ◽

Condensed Tannins ◽

Physiological Function ◽

Animal Feed ◽

Molecular Genetic ◽

Flavonoid Biosynthesis ◽

Human Consumption ◽

Genetic Studies ◽

Plant Seeds ◽

Reproductive Structures

AbstractFlavonoids are secondary metabolites that are present at high levels in most plant seeds and grains. These compounds appear to play vital roles in defence against pathogens and predators and contribute to physiological functions such as seed maturation and dormancy. At the same time, particular subclasses of flavonoids, such as the proanthocyanidins (condensed tannins), negatively impact the use of seeds and grains in animal feed and can add undesirable qualities to food products for human consumption. This article presents an overview of investigations into the physiological and agronomic aspects of seed and grain flavonoids as well as a review of molecular genetic studies, particularly in maize,Arabidopsisand soybean, where mutants deficient in flavonoid biosynthesis provide useful tools for stydying the metabolic machinery underlying the accumulation of these compounds in reproductive structures.

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