scholarly journals Differences in the Abundance of Auxin Homeostasis Proteins Suggest Their Central Roles for In Vitro Tissue Differentiation in Coffea arabica

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2607
Author(s):  
Ana O. Quintana-Escobar ◽  
Hugo A. Méndez-Hernández ◽  
Rosa M. Galaz-Ávalos ◽  
José M. Elizalde-Contreras ◽  
Francisco A. Reyes-Soria ◽  
...  
Keyword(s):  
Coffea Arabica ◽  
Suspension Cultures ◽  
Indole Butyric Acid ◽  
Auxin Homeostasis ◽  
Tandem Mass Tag ◽  
Conservation Genetic ◽  
Auxin Binding ◽  
Auxin Binding Protein ◽  
Indole 3 Acetic Acid

Coffea arabica is one of the most important crops worldwide. In vitro culture is an alternative for achieving Coffea regeneration, propagation, conservation, genetic improvement, and genome editing. The aim of this work was to identify proteins involved in auxin homeostasis by isobaric tandem mass tag (TMT) and the synchronous precursor selection (SPS)-based MS3 technology on the Orbitrap Fusion™ Tribrid mass spectrometer™ in three types of biological materials corresponding to C. arabica: plantlet leaves, calli, and suspension cultures. Proteins included in the β-oxidation of indole butyric acid and in the signaling, transport, and conjugation of indole-3-acetic acid were identified, such as the indole butyric response (IBR), the auxin binding protein (ABP), the ATP-binding cassette transporters (ABC), the Gretchen-Hagen 3 proteins (GH3), and the indole-3-acetic-leucine-resistant proteins (ILR). A more significant accumulation of proteins involved in auxin homeostasis was found in the suspension cultures vs. the plantlet, followed by callus vs. plantlet and suspension culture vs. callus, suggesting important roles of these proteins in the cell differentiation process.

Interaction of auxin-binding protein 1 with maize coleoptile plasma membranes in vitro

Planta ◽  
1997 ◽  
Vol 201 (4) ◽  
pp. 470-476 ◽  
Author(s):  
Christine Schiebl ◽  
Antje Walther ◽  
Ursula Rescher ◽  
Dieter Klämbt
Keyword(s):  
Plasma Membranes ◽  
Binding Protein ◽  
Maize Coleoptile ◽  
Auxin Binding ◽  
Auxin Binding Protein

An Auxin Binding Protein is Localized in the Symbiosome Membrane of Soybean Nodules

1993 ◽  
Vol 48 (1-2) ◽  
pp. 35-40 ◽  
Author(s):  
Andreas Jacobi ◽  
Rolf Zettl ◽  
Klaus Palme ◽  
Dietrich Werner
Keyword(s):  
Acetic Acid ◽  
Auxin Transport ◽  
Binding Protein ◽  
Naphthaleneacetic Acid ◽  
Dependent Manner ◽  
Symbiosome Membrane ◽  
Auxin Binding ◽  
Auxin Binding Protein ◽  
Triton X ◽  
Indole 3 Acetic Acid

Binding of tritiated indole-3-acetic acid ([3H]IAA) to symbiosome membranes of soybean nodules occurred in a protein-dependent manner and was competitively inhibited by unlabeled indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (1-NAA) and dithiothreitol (DTT), but not by tryptophan and benzoic acid. The symbiosome membranes bound IAA with a KD of 1 × 10-6 m. Photoaffinity labeling identified an auxin-binding protein (ABP) in the symbiosome membrane with an apparent molecular mass of 23 kDa. This 23 kDa protein was labeled either with 5-azido-[7-3H]indole-3-acetic acid ([3H]N3IAA) or with 5′-azido-[3,6-3H2]-1-naphthylphthalamic acid ([3H2]N3NPA). Labeling of the 23 kDa protein with [3H]N3IAA was competitively inhibited by unlabeled IAA and 1-NAA. NPA and quercetin, inhibitors of polar auxin transport, as well as rutin, a glycosylated derivative of quercetin, competed with IAA for binding. Conversely, [3H2]N3NPA labeling was inhibited by unlabeled IAA and NPA. The 23 kDa symbiosome membrane protein was partially solubilized with Triton X-100 and nearly completely using Triton X-114. The observation that auxin transport inhibitors compete with IAA for binding suggests that the symbiosome membrane ABP could be part of an auxin efflux carrier system required to control the auxin concentration in infected soybean nodule cells.

scholarly journals Long-Term Subculture Affects Rooting Competence via Changes in the Hormones and Protein Profiles in Cedrela Fissilis Vell. (Meliaceae) Shoots

2021 ◽  
Author(s):  
Tadeu dos Reis Oliveira ◽  
Damián Balfagón ◽  
Kariane Rodrigues Sousa ◽  
Victor Paulo Mesquita Aragão ◽  
Leandro Francisco de Oliveira ◽  
...  
Keyword(s):  
Large Scale ◽  
Molecular Mechanisms ◽  
Monodehydroascorbate Reductase ◽  
Protein Profiles ◽  
Ex Vitro ◽  
Plantlet Production ◽  
Auxin Binding Protein ◽  
Indole 3 Acetic Acid

Abstract Long-term subculture plays an essential role in the large-scale multiplication and production of somatic plantlets. We investigated the effects of long-term subculture on in vitro shoot development and ex vitro rooting associated with changes in the hormones and protein profiles in C. fissilis. The number of subcultures of shoots induced a decrease in the ex vitro rooting response. The reduction in adventitious root (AR) formation was associated with decreases in the contents of indole-3-acetic acid (IAA), abscisic acid (ABA), 12-oxo phytodienoic acid (OPDA), putrescine (Put), and spermine and increases in jasmonic acid (JA), jasmonoyl-isoleucine, trans-cinnamic acid, and salicylic acid contents in shoots at the fourth subculture compared to the first. The ornithine decarboxylase enzyme preferentially functions in the Put biosynthesis pathway and was related to the highest AR formation in shoots at the first subculture. Down-accumulation of the auxin-binding protein ABP19a in shoots from the fourth subculture compared to the first subculture was related to a decrease in both IAA contents and AR formation. In addition, down-accumulation of glucose-6-phosphate isomerase, glutamine synthetase leaf isozyme chloroplastic, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, L-ascorbate peroxidase, cytosolic, monodehydroascorbate reductase, and 2-Cys peroxiredoxin BAS1-like, chloroplastic and up-accumulation of caffeoyl-CoA O-methyltransferase 1 and isoforms of peroxidase 4 proteins in shoots from the fourth relative to the first subculture were associated with a reduction in AR formation. These results showed that the understanding of hormonal and molecular mechanisms related to the potential of AR formation in shoots under successive subcultures is relevant to improving large-scale plantlet production in C. fissilis.

A soluble auxin-binding protein from cultured tobacco tissues stimulates RNA synthesis in vitro

Planta ◽  
1984 ◽  
Vol 160 (2) ◽  
pp. 102-108 ◽  
Author(s):  
P. C. G. van der Linde ◽  
H. Bouman ◽  
A. M. Mennes ◽  
K. R. Libbenga
Keyword(s):  
Rna Synthesis ◽  
Binding Protein ◽  
Auxin Binding ◽  
Auxin Binding Protein
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