scholarly journals In Vitro Propagation of Gastrochilus matsuran (Makino) Schltr., an Endangered Epiphytic Orchid

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 524 ◽  
Author(s):  
Hyeonjeong Kang ◽  
Kyung Won Kang ◽  
Doo Hwan Kim ◽  
Iyyakkannu Sivanesan
Keyword(s):  
Seed Germination ◽  
Mass Production ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Natural Habitat ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Epiphytic Orchid ◽  
Protocorm Formation

Gastrochilus matsuran (Makino) Schltr. (Orchidaceae) populations are declining quickly because of overexploitation, climatic changes, and deforestation; therefore, mass-production protocols are required for this orchid. Natural propagation of this species is often hampered by meager seed germination and slow growth. Thus, our aim was to establish an effective protocol for the in vitro propagation of G. matsuran and reduce the risk of its extinction. We investigated the impacts of culture media, coconut water (CW), and plant hormones (gibberellic acid (GA3), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA), and thidiazuron (TDZ)) on asymbiotic germination, multiplication and conversion of protocorms, and plantlet development. Maximal seed germination (93.3%) was achieved on ½ MS medium without vitamins plus 5% CW, 1 µM NAA, and 1.5 µM GA3. Secondary protocorm formation was best achieved on ½ MS medium without vitamins plus 2 µM TDZ. The conversion of protocorms into seedlings was maximized by supplementation with 2 µM IBA or 1 µM NAA. Acclimatized plantlets that exhibited exuberant growth on sphagnum moss were reintroduced to tree trunks in a natural habitat, with a 67% survival rate. This in vitro propagation procedure would be helpful for the mass production and conservation of this rare epiphytic orchid.

scholarly journals Micropropagation and in vitro conservation of Alcantarea nahoumii (Bromeliaceae), an endemic and endangered species of the Brazilian Atlantic Forest

2020 ◽  
Vol 42 ◽  
pp. e52940
Author(s):  
Simone Sacramento dos Santos Silva ◽  
Everton Hilo de Souza ◽  
Fernanda Vidigal Duarte Souza ◽  
Cristina Ferreira Nepomuceno ◽  
Maria Angélica Pereira de Carvalho Costa
Keyword(s):  
Atlantic Forest ◽  
Culture Media ◽  
In Vitro Conservation ◽  
Naphthaleneacetic Acid ◽  
High Survival ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Mature Seeds ◽  
Stem Segments

Alcantarea nahoumii (Leme) J. R. Grant is a species native to the Atlantic Forest that stands out for ornamental purposes. The objective of this work was to evaluate the in vitro germination of A. nahoumii seeds and establish a micropropagation protocol for production of seedlings so as to minimize the effects of predatory extractivism and develop an in vitro conservation system. Mature seeds were disinfested, established in three culture media (MS, MS½ and MS⅓) and incubated at four temperatures (20, 25, 30 and 35ºC) in a germination chamber. In the micropropagation experiment, stem segments were introduced in MS medium supplemented with 0.5 μM of 1-naphthaleneacetic acid (NAA) and 0.0, 2.2, 4.4 and 6.6 μM of 6-benzylaminopurine (BAP). For the in vitro conservation, plantlets were established in MS or MS½ medium supplemented with 15 g L-1 or 30 g L-1 of sucrose. The plants were acclimated with commercial substrate. The highest seed germination percentages were promoted by temperature conditions of 20 and 25ºC, with MS culture medium. The highest multiplication rate of shoots was obtained from the treatment without addition of the growth regulator or when combined with 2.2 μM of BAP + 0.5 μM of NAA. The acclimation of the plants occurred with high survival rate. The species can be conserved in vitro under slow growth condition for 24 months when incubated in MS medium supplemented with 30 g L-1 of sucrose.

scholarly journals In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

1970 ◽  
Vol 9 (9) ◽  
pp. 50-52 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha ◽  
Shreeti Pradhan
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ideal Condition ◽  
Ms Medium ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

scholarly journals The Response of in Vitro Propagation of Marumi Kumquat (fortunella Japonica Thunb.) to Different Culture Media, Plant Growth Regulators And Different Fructose Concentration

2020 ◽  
Vol 23 (1) ◽  
pp. 178-190
Author(s):  
Jeillan Hussein ◽  
Diaa ibraheam
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Nutritional Value ◽  
Culture Media ◽  
Shoot Length ◽  
Ms Medium ◽  
Surface Sterilization

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

scholarly journals In Vitro Propagation of Croton Scabiosus Bedd. (Euphorbiaceae), an Endemic and Vulnerable Tree Species

2013 ◽  
Vol 3 (3) ◽  
pp. 229-241
Author(s):  
B. Ravi Prasad Rao ◽  
S. Salamma
Keyword(s):  
Tree Species ◽  
In Vitro Propagation ◽  
Andhra Pradesh ◽  
Natural Habitat ◽  
Eastern Ghats ◽  
Ms Medium ◽  
Species Population ◽  
Half Strength ◽  
Site Conservation

Croton scabiosus Bedd. (Euphorbiaceae), an endemic tree species of Southern Eastern Ghats of Andhra Pradesh, India, categorised as ‘Vulnerable’ is attempted for in vitro propagation. Seed pathological problems, poor germination, recurrent fires and tree fellings in the native habitat are the major threats of the species. With an objective to standardise a suitable multiplication protocol to augment the species population in the natural habitat, the present study focus on multiplication of the species through micropropagation. Of the various growth regulators employed in the experimentation, maximum mean number of shoots i.e. 5.37±0.12 was found on MS medium fortified with 0.5mg/l BAP and 2.5mg/l IAA. Maximum mean length of shoots 5.27±0.14 was found on 0.5mg/l BAP+1mg/l IAA. In vitro rooting was found on half strength MS medium fortified with 2.5mg/l IBA (8.92±0.19 mean number of roots with 5.04±0.05 mean length of roots with 90% response). Only 10% of survival rate was observed and the species warrants effective on site conservation methods.

scholarly journals Micropropagation of Liriope muscari via leaf explant

1969 ◽  
Vol 83 (3-4) ◽  
pp. 169-173
Author(s):  
Keithley L. Amory ◽  
John M. Gill
Keyword(s):  
Acetic Acid ◽  
Mass Production ◽  
In Vitro Propagation ◽  
Solid Medium ◽  
Leaf Explants ◽  
Ms Medium ◽  
Isopentenyl Adenine ◽  
Young Leaves ◽  
Dichlorophenoxy Acetic Acid

Young leaves of Liriope muscari provide an ample source of explants for in vitro propagation in tropical countries where flowering is scarce. Leaves were induced to form calli on a solid medium containing Murashige and Skoog (MS) salts and vitamins, 3% sucrose, 0.7% agar, 1 mg/L 2,4-dichlorophenoxy- acetic acid (2, 4-D) and 1 mg/L 6-furfurylaminopurine (kinetin). Only the proximal segments of the leaves produced calli. These calli were induced to produce multiple plantlets on MS medium, 3% sucrose, 0.7% agar, and 10 mg/L N6 (2-isopentenyl) adenine (2 ip). It is possible to use leaf explants for in vitro mass production of Liriope. However, in variegated varieties, only green or white plants were produced, because of a chimera in the original tissue.

scholarly journals In vitro seed germination in Cymbidium elegans Lindl. and Dendrobium densiflorum Lindl. ex Wall. (Orchidaceae)

2010 ◽  
Vol 6 ◽  
pp. 100-102 ◽  
Author(s):  
Shreeti Pradha ◽  
Bijaya Pant
Keyword(s):  
Seed Germination ◽  
Basal Medium ◽  
Naphthalene Acetic Acid ◽  
Genetic Constitution ◽  
Ms Medium ◽  
Orchid Species ◽  
C Elegans ◽  
Protocorm Formation ◽  
Dendrobium Densiflorum

A comparative study of in vitro seed germination of two endangered orchid species, viz. Cymbidium elegans Lindl. and Dendrobium densiflorum Lindl. ex Wall., was carried out on Murashige and Skoog's (MS) medium, supplemented with different concentrations and combination of 6-benzylaminopurine (BAP) and á-Naphthalene acetic acid (NAA). The hormone-free MS medium and MS medium supplemented with various growth hormones were found effective for in vitro seed germination of both species. However, the seeds of these two species showed variation in their germination behavior. Hormone-free MS basal medium was found most effective for seed germination of D. densiflorum; whereas, basal medium supplemented with BAP (1mg/l) was effective for C. elegans. The seeds of D. densiflorum showed quick response in earlier germination, protocorm formation and further development into seedlings in comparison to C. elegans. In C. elegans, germination of immature seeds started after nine weeks of inoculation; whereas in D. densiflorum, the initiation of germination started after five weeks of culture. The variations in seed germination, protocorm formation and seedling differentiation in the two orchid species might be due to the differences in their genetic constitution and the presence of different endogenous growth stimulating substances present in their seeds. The present study has provided useful information for in vitro clonal mass multiplication of these commercially important orchid species. Key-words: growth hormone; in vitro study; orchid.DOI: 10.3126/botor.v6i0.2917 Botanica Orientalis - Journal of Plant Science (2009) 6: 100-102

scholarly journals Challenges and Opportunity of in vitro Propagation of Paulownia tomentosa Steud for Commercial Production in Nepal

2016 ◽  
Vol 4 (2) ◽  
pp. 155-160
Author(s):  
Lila Bahadur Magar ◽  
Nisha Shrestha ◽  
Saraswoti Khadka ◽  
Jay Raj Joshi ◽  
Jibaraj Acharya ◽  
...  
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Royalty Rate ◽  
Paulownia Tomentosa ◽  
Shoot Bud ◽  
Plastic Bags

Paulownia tomentosa Steud is a fast growing multipurpose tree. In vitro propagation using nodal explants of Paulownia tomentosa was performed by manipulating amount of cytokinin and auxin in culture media. Shoot bud proliferation from explants were assessed in Murashige and Skoog (MS) medium supplemented with various amounts of hormones such as a) 0.5-2.5 mg/l benzylaminopurine (BAP) and 0.1 mg/l naphthalene acetic acid (NAA), b) 0.5-2.5 mg/l BAP and 0.5-2.5 mg/l kinetin (KN) and c) 0.5- 2.5 mg/l BAP and 0.1 mg/l indole-3-acetic acid (IAA). In the present study, we found that hormone combination of BAP and NAA gave optimum growth results. MS medium enriched with 2.0 mg/l BAP and 0.1 mg/l IAA resulted a similar outcome but took 3-4 weeks with respect to the same medium enriched with 1.0 mg/ml BAP and 0.1 mg/ml NAA, which showed response within 2-3 weeks. Shoot length of 2.5-3.5 cm with 3-4 nodes and 8-12 leaves were obtained on MS medium supplemented with 1.0 mg/l BAP and 0.1 mg/l NAA. The acclimatization of explants was done in a polyhouse at 20±5oC for 2-6 weeks. Rooting has been induced in nonsterile sand. Rooted plants were transferred to plastic bags containing mixture of soil, sand and compost in the ratio of 1:1:1.Besides aforementioned issues, there are several other challenges associated with in vitro propagation of P. tomentosa. The plants were established (90%) on MS medium enriched with BAP and NAA and adapted ex vitro with surviving up to 80%. People received an opportunity with this plant because it grows fast and can generate income in 10 years in comparison with others, but at the same time people also have been facing the challenges for plantation of P. tomentosa as government of Nepal does not formulate necessary national policies, legislations and regulatory frameworks in its favor. Thus, system should be developed to set royalty rate of P. tomentosa recognizing its lifetime value.Int J Appl Sci Biotechnol, Vol 4(2): 155-160

scholarly journals Clonal Micropropagation of representatives of the genus Dactylorhiza Neck. ex Nevski

2021 ◽  
Vol 4 (46) ◽  
pp. 17-17
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Survival Rate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Culture Media ◽  
Ms Medium ◽  
Organic Additives ◽  
Clonal Micropropagation ◽  
Half Strength

Abstract The aim of this study is to develop and improve methods of in vitro propagation of representatives of Dactylorhiza: D.baltica , D. fuchsii. For the study, we used protocorms obtained by the asymbiotic germination of seed during 90 days. It has been established that half-strength of Murashige and Skoog (1962) medium (½ MS) supplemented with 1-2 mg/l 6-Benzylaminopurine(6-BAP), potato puree (20g/l), and charcoal (1g/l) effectively influenced the development of protocorms, and seedlings formation in the studied species. The result of the study showed that the survival rate of protocorms was high in all experimental culture media, but in D. fuchsii it was better at a concentration 2mg/l of 6-BAP (95.4%), while in D. baltica it was high at 1mg/l (87.0%). The highest percentage of multiple protocorms (68%) and the formation of new secondary protocorms in D. fuchsii (5,5±0,3 units) were observed on a culture medium containing 2 mg/l 6-BAP. The highest percent of rooting of D. fuchsii protosoms (78%) and length of roots (0.9cm) observed in ½ MS medium without growth regulators. During the development of D. baltica protosoms, the culture medium of ½ MS containing 1 mg/l 6-BAP had the best effect on the number of roots (1.8±0.1root/protosom), while the medium supplemented with 2mg/l of 6-BAP contributed to the formation of a larger number of new secondary protocorms (3,2±0,1protocorm/unit). During the subsequent cultivation of protosoms of D. baltica on a culture medium containing 1 mg/l it was observed an increase in the height of shoots (4,8±0,3 см), and the length of roots (2,2±0,1 см), wherein the number of newly formed protocorms was higher by 30% on the medium supplemented with 2 mg/l 6-BAP. Keywords: DACTYLORHIZA BALTICA, DACTYLORHIZA FUCHSII, IN VITRO, PROTOCORMS, ORGANIC ADDITIVES

scholarly journals Plant Regeneration from Leaf Explants of the Medicinal Herb Wedelia chinensis

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 407
Author(s):  
Yung-Ting Tsai ◽  
Kin-Ying To
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Liver Toxicity ◽  
Leaf Explants ◽  
Basal Medium ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Average Percentage

Wedelia chinensis, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reported, including through nodal segments, axillary buds, and shoot tips, whereas leaf segments failed to proliferate. Here, we report on the in vitro propagation of W. chinensis by culturing young leaf explants in MS medium supplemented with 0.5 mg/L α-naphthaleneacetic acid (NAA), 0.75 mg/L thidiazuron (TDZ), 1 mg/L gibberellic acid (GA3), 3.75 mg/L adenine, 3% sucrose, and 0.8% agar at pH 5.8. Calli were observed in all explants derived from the youngest top two leaves, and the average percentage of shoot regeneration was 23% from three independent experiments. Then, several shoots were excised, transferred onto MS basal medium supplemented with 3% sucrose and 0.8% agar at pH 5.8, and cultured in a growth chamber for 1 to 2 months. Roots were easily induced. Finally, plantlets carrying shoots and roots were transferred into soil, and all of them grew healthily in a greenhouse. No morphological variation was observed between the regenerated plantlets and the donor wild-type plants. In addition, we also established root cultures of W. chinensis in culture medium (MS medium, 3 mg/L NAA, 3% sucrose, pH 5.8) with or without 0.8% agar. To the best of our knowledge, this is the first paper reporting plant regeneration from leaf explants in the herbal plant W. chinensis.

scholarly journals EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS

2020 ◽  
Vol 19 (4) ◽  
pp. 41-51
Author(s):  
Tour Jan ◽  
Ikram Ullah ◽  
Bilal Muhammad ◽  
_ Tariq ◽  
Ali Mansoor ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Ammonium Nitrate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Dark Green

Hyperhydricity is a frequently problem in plants during in vitro culture and affected micropropagation ofplants. To develop an efficient in vitro regenerated system without hyperdydricity, we demonstrated the effectof different disinfected agents (mercuric chlorite and hypochlorite), growth regulators, their concentrationsand combinations, Agar, pH, ammonium nitrate (NH4NO3) and number of subcultures. Mercuric chlorite at0.07% and exposing time (9–10 min) was appropriate for hygienic culture. The shoots induced by Benzyladnine(BA) alone or in combination with α-Naphthaleneacetic acid (NAA) exhibited maximum multiplicationwith symptoms of hyperhydricity than those induced by Kinetin alone or in combination with NAA. Hyperhydricitywas also reduced by increasing the concentration of agar, pH and elimination of NH4NO3 from themacroelements of Murashig and Skoog (MS) medium. Repeated subcultures affected both multiplication andhyperhydricity. The multiplication of shoots increased from parental culture up to 5th subculture and thereafterdeclined in 6th subculture. Although shoot hyperhydricity were observed from 1st subculture (19%) andthen increased up to 85% in 6th subculture. This increased in hyperhydricity could be due to the remaininginfluence of hormones. In shoots of 5th subculture the content of chlorophyll (dark green) were higher thanshoots of 6th subculture.

Sign in / Sign up

Export Citation Format

Share Document