scholarly journals Characterisation of Rapid In Situ Forming Gelipin Hydrogel for Future Use in Irregular Deep Cutaneous Wound Healing

Polymers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 3152
Author(s):  
Dewi Utami Nike ◽  
Haliza Katas ◽  
Nor Fatimah Mohd ◽  
Yosuke Hiraoka ◽  
Yasuhiko Tabata ◽  
...  
Keyword(s):  
Wound Healing ◽  
Room Temperature ◽  
Dermal Fibroblasts ◽  
Grand Challenge ◽  
Cutaneous Wounds ◽  
Normal Wound Healing ◽  
In Situ Forming ◽  
Swelling Capacity ◽  
Biocompatibility Test

The irregular deep chronic wound is a grand challenge to be healed due to multiple factors including slow angiogenesis that causing regenerated tissue failure. The narrow gap of deep wounds could hinder and slow down normal wound healing. Thus, the current study aimed to develop a polymerised genipin-crosslinked gelatin (gelipin) hydrogel (GNP_GH) as a potential biodegradable filler for the abovementioned limitations. Briefly, GNP_GH bioscaffolds have been developed successfully within three-minute polymerisation at room temperature (22–24 °C). The physicochemical and biocompatibility of GNP_GH bioscaffolds were respectively evaluated. Amongst GNP_GH groups, the 0.1%GNP_GH10% displayed the highest injectability (97.3 ± 0.6%). Meanwhile, the 0.5%GNP_GH15% degraded within more than two weeks with optimum swelling capacity (108.83 ± 15.7%) and higher mechanical strength (22.6 ± 3.9 kPa) than non-crosslinked gelatin hydrogel 15% (NC_GH15%). Furthermore, 0.1%GNP_GH15% offered higher porosity (˃80%) and lower wettability (48.7 ± 0.3) than NC_GH15%. Surface and cross-section SEM photographs displayed an interconnected porous structure for all GNP_GH groups. The EDX spectra and maps represented no major changes after GNP modification. Moreover, no toxicity effect of GNP_GH against dermal fibroblasts was shown during the biocompatibility test. In conclusion, the abovementioned findings indicated that gelipin has excellent physicochemical properties and acceptable biocompatibility as an acellular rapid treatment for future use in irregular deep cutaneous wounds.

scholarly journals Forming pressure effect on microstructure and mechanical properties of nanocrystalline aluminum synthesized by inert gas condensation

2019 ◽  
Vol 79 ◽  
pp. 02002
Author(s):  
Shangshu Wu ◽  
Zhou Yu ◽  
Junjie Wang ◽  
Hanxin Zhang ◽  
Chaoqun Pei ◽  
...  
Keyword(s):  
Room Temperature ◽  
High Vacuum ◽  
Inert Gas ◽  
Helium Atmosphere ◽  
Gas Condensation ◽  
Inert Gas Condensation ◽  
In Situ Forming ◽  
Nanocrystalline Aluminum ◽  
Al Powder

The preparation of nanocrystalline aluminum (NC Al) was conducted in two steps. After the NC Al powder was synthesized by an Inert gas condensation (IGC) method in a helium atmosphere of 500 Pa, the NC Al powder was in-situ compacted into a pellet with a 10 mm diameter and 250 μm-300 μm thickness in a high vacuum (10-6 Pa-10-7 Pa) at room temperature. The NC Al samples were not exposed to air during the entire process. After the pressure reached 6 GPa, the relative density could reach 99.83%. The results showed that the grain size decreased with the increased of in-situ forming pressure. The NC Al samples present obvious ductile fracture, and the tensile properties were greatly changed with the increase of forming pressure.

Moist-Retaining, Self-Recoverable, Bioadhesive, and Transparent in Situ Forming Hydrogels To Accelerate Wound Healing

2020 ◽  
Vol 12 (2) ◽  
pp. 2023-2038 ◽  
Author(s):  
Jun Li ◽  
Fan Yu ◽  
Gong Chen ◽  
Jia Liu ◽  
Xiao-Long Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Accelerate Wound Healing ◽  
In Situ Forming ◽  
In Situ Forming Hydrogels

In Situ Forming and Rutin-Releasing Chitosan Hydrogels As Injectable Dressings for Dermal Wound Healing

2011 ◽  
Vol 12 (8) ◽  
pp. 2872-2880 ◽  
Author(s):  
Ngoc Quyen Tran ◽  
Yoon Ki Joung ◽  
Eugene Lih ◽  
Ki Dong Park
Keyword(s):  
Wound Healing ◽  
Dermal Wound Healing ◽  
In Situ Forming ◽  
Chitosan Hydrogels ◽  
Dermal Wound

In situ forming and reactive oxygen species-scavenging gelatin hydrogels for enhancing wound healing efficacy

2020 ◽  
Vol 103 ◽  
pp. 142-152 ◽  
Author(s):  
Phuong Le Thi ◽  
Yunki Lee ◽  
Dieu Linh Tran ◽  
Thai Thanh Hoang Thi ◽  
Jeon Il Kang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wound Healing ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
In Situ Forming

Enzyme-catalyzed in situ forming gelatin hydrogels as bioactive wound dressings: effects of fibroblast delivery on wound healing efficacy

2014 ◽  
Vol 2 (44) ◽  
pp. 7712-7718 ◽  
Author(s):  
Yunki Lee ◽  
Jin Woo Bae ◽  
Jin Woo Lee ◽  
Wonhee Suh ◽  
Ki Dong Park
Keyword(s):  
Wound Healing ◽  
Wound Dressings ◽  
Wound Treatment ◽  
In Situ Forming

Wound treatment using injectable or sprayable fibroblast-encapsulated GH-hydrogels.

scholarly journals Development of an in-situ forming, self-healing scaffold for dermal wound healing: in-vivo and in-vivo studies

2021 ◽  
pp. 112263
Author(s):  
Swati Sharma ◽  
Harishkumar Madhyastha ◽  
K. Laxmi Swetha ◽  
Kavya Sree Maravajjala ◽  
Archana Singh ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vivo Studies ◽  
Self Healing ◽  
Dermal Wound Healing ◽  
In Situ Forming ◽  
Dermal Wound

scholarly journals NGF Accelerates Cutaneous Wound Healing by Promoting the Migration of Dermal Fibroblasts via the PI3K/Akt-Rac1-JNK and ERK Pathways

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Ji-Cai Chen ◽  
Bei-Bei Lin ◽  
Hou-Wen Hu ◽  
Cai Lin ◽  
Wen-Yang Jin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Immunohistochemical Staining ◽  
Randomized Clinical Trials ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Cutaneous Wound ◽  
Cutaneous Wounds ◽  
Fibroblast Migration ◽  
Underlying Mechanisms ◽  
Specific Inhibitors

As a well-known neurotrophic factor, nerve growth factor (NGF) has also been extensively recognized for its acceleration of healing in cutaneous wounds in both animal models and randomized clinical trials. However, the underlying mechanisms accounting for the therapeutic effect of NGF on skin wounds are not fully understood. NGF treatment significantly accelerated the rate of wound healing by promoting wound reepithelialization, the formation of granulation tissue, and collagen production. To explore the possible mechanisms of this process, the expression levels of CD68, VEGF, PCNA, and TGF-β1 in wounds were detected by immunohistochemical staining. The levels of these proteins were all significantly raised in NGF-treated wounds compared to untreated controls. NGF also significantly promoted the migration, but not the proliferation, of dermal fibroblasts. NGF induced a remarkable increase in the activity of PI3K/Akt, JNK, ERK, and Rac1, and blockade with their specific inhibitors significantly impaired the NGF-induced migration. In conclusion, NGF significantly accelerated the healing of skin excisional wounds in rats and the fibroblast migration induced by NGF may contribute to this healing process. The activation of PI3K/Akt, Rac1, JNK, and ERK were all involved in the regulation of NGF-induced fibroblast migration.

scholarly journals In Situ Crosslinking Bionanocomposite Hydrogels with Potential for Wound Healing Applications

2019 ◽  
Vol 10 (4) ◽  
pp. 50 ◽  
Author(s):  
Federica Leone ◽  
Melike Firlak ◽  
Kirsty Challen ◽  
Wayne Bonnefin ◽  
Barbara Onida ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vitro Release ◽  
Patient Specific ◽  
Hacat Cells ◽  
In Situ Gel ◽  
In Situ Forming ◽  
In Situ Forming Hydrogels ◽  
In Situ Crosslinking

In situ forming hydrogels are a class of biomaterials that can fulfil a variety of important biomedically relevant functions and hold promise for the emerging field of patient-specific treatments (e.g., cell therapy, drug delivery). Here we report the results of our investigations on the generation of in situ forming hydrogels with potential for wound healing applications (e.g., complex blast injuries). The combination of polysaccharides that were oxidized to display aldehydes, amine displaying chitosan and nanostructured ZnO yields in situ forming bionanocomposite hydrogels. The physicochemical properties of the components, their cytotoxicity towards HaCat cells and the in vitro release of zinc ions on synthetic skin were studied. The in situ gel formation process was complete within minutes, the components were non-toxic towards HaCat cells at functional levels, Zn2+ was released from the gels, and such materials may facilitate wound healing.

Sign in / Sign up

Export Citation Format

Share Document